Kizina, Kathrin M.

[["dc.bibliographiccitation.journal","Acta Physiologica"],["dc.bibliographiccitation.volume","216"],["dc.contributor.author","Terwitte, L."],["dc.contributor.author","Kizina, K."],["dc.contributor.author","Mueller, M."],["dc.date.accessioned","2018-11-07T10:17:30Z"],["dc.date.available","2018-11-07T10:17:30Z"],["dc.date.issued","2016"],["dc.identifier.isi","000372285400300"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/41238"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.publisher.place","Hoboken"],["dc.relation.issn","1748-1716"],["dc.relation.issn","1748-1708"],["dc.title","Quantitative redox imaging in complex preparations by dual-laser based excitation ratiometric 2-photon microscopy"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","202"],["dc.bibliographiccitation.journal","Acta Physiologica"],["dc.bibliographiccitation.lastpage","204"],["dc.bibliographiccitation.volume","210"],["dc.contributor.author","Mueller, M."],["dc.contributor.author","Kizina, K."],["dc.contributor.author","Bao, Guobin"],["dc.date.accessioned","2018-11-07T09:42:49Z"],["dc.date.available","2018-11-07T09:42:49Z"],["dc.date.issued","2014"],["dc.identifier.isi","000332259900526"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/34042"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.publisher.place","Hoboken"],["dc.relation.issn","1748-1716"],["dc.relation.issn","1748-1708"],["dc.title","Advanced ROS/redox imaging based on genetically-encoded probes, ratiometric 2-photon microscopy and fluorescence-lifetime imaging"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","227"],["dc.bibliographiccitation.journal","Free Radical Biology & Medicine"],["dc.bibliographiccitation.lastpage","241"],["dc.bibliographiccitation.volume","76"],["dc.contributor.author","Weller, Jonathan"],["dc.contributor.author","Kizina, Kathrin M."],["dc.contributor.author","Can, Karolina"],["dc.contributor.author","Bao, Guobin"],["dc.contributor.author","Müller, Michael"],["dc.date.accessioned","2018-09-28T09:56:42Z"],["dc.date.available","2018-09-28T09:56:42Z"],["dc.date.issued","2014"],["dc.description.abstract","Reactive oxygen species mediate cellular signaling and neuropathologies. Hence, there is tremendous interest in monitoring (sub)cellular redox conditions. We evaluated the genetically engineered redox sensor HyPer in mouse hippocampal cell cultures. Two days after lipofection, neurons and glia showed sufficient expression levels, and H2O2 reversibly and dose-dependently increased the fluorescence ratio of cytosolic HyPer. Yet, repeated H2O2 treatment caused progressively declining responses, and with millimolar doses an apparent recovery started while H2O2 was still present. Although HyPer should be H2O2 specific, it seemingly responded also to other oxidants and altered cell-endogenous superoxide production. Control experiments with the SypHer pH sensor confirmed that the HyPer ratio responds to pH changes, decreasing with acidosis and increasing during alkalosis. Anoxia/reoxygenation evoked biphasic HyPer responses reporting apparent reduction/oxidation; replacing Cl(-) exerted only negligible effects. Mitochondria-targeted HyPer readily responded to H2O2-albeit less intensely than cytosolic HyPer. With ratiometric two-photon excitation, H2O2 increased the cytosolic HyPer ratio. Time-correlated fluorescence-lifetime imaging microscopy (FLIM) revealed a monoexponential decay of HyPer fluorescence, and H2O2 decreased fluorescence lifetimes. Dithiothreitol failed to further reduce HyPer or to induce reasonable FLIM and two-photon responses. By enabling dynamic recordings, HyPer is superior to synthetic redox-sensitive dyes. Its feasibility for two-photon excitation also enables studies in more complex preparations. Based on FLIM, quantitative analyses might be possible independent of switching excitation wavelengths. Yet, because of its pronounced pH sensitivity, adaptation to repeated oxidation, and insensitivity to reducing stimuli, HyPer responses have to be interpreted carefully. For reliable data, side-by-side pH monitoring with SypHer is essential."],["dc.identifier.doi","10.1016/j.freeradbiomed.2014.07.045"],["dc.identifier.pmid","25179473"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/15849"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation.eissn","1873-4596"],["dc.title","Response properties of the genetically encoded optical H2O2 sensor HyPer"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","41"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Antioxidants & Redox Signaling"],["dc.bibliographiccitation.lastpage","58"],["dc.bibliographiccitation.volume","25"],["dc.contributor.author","Wagener, Kerstin C."],["dc.contributor.author","Kolbrink, Benedikt"],["dc.contributor.author","Dietrich, Katharina"],["dc.contributor.author","Kizina, Kathrin M."],["dc.contributor.author","Terwitte, Lukas S."],["dc.contributor.author","Kempkes, Belinda"],["dc.contributor.author","Bao, Guobin"],["dc.contributor.author","Müller, Michael"],["dc.date.accessioned","2018-09-28T09:52:54Z"],["dc.date.available","2018-09-28T09:52:54Z"],["dc.date.issued","2016"],["dc.description.abstract","Reactive oxygen species (ROS) and downstream redox alterations not only mediate physiological signaling but also neuropathology. For long, ROS/redox imaging was hampered by a lack of reliable probes. Genetically encoded redox sensors overcame this gap and revolutionized (sub)cellular redox imaging. Yet, the successful delivery of sensor-coding DNA, which demands transfection/transduction of cultured preparations or stereotaxic microinjections of each subject, remains challenging. By generating transgenic mice, we aimed to overcome limiting cultured preparations, circumvent surgical interventions, and to extend effectively redox imaging to complex and adult preparations."],["dc.identifier.doi","10.1089/ars.2015.6587"],["dc.identifier.pmid","27059697"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/15848"],["dc.language.iso","en"],["dc.notes.status","zu prüfen"],["dc.relation.eissn","1557-7716"],["dc.title","Redox Indicator Mice Stably Expressing Genetically Encoded Neuronal roGFP: Versatile Tools to Decipher Subcellular Redox Dynamics in Neuropathophysiology"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]