Kliesch, Torben-Tobias

[["dc.bibliographiccitation.firstpage","388"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Strahlentherapie und Onkologie"],["dc.bibliographiccitation.lastpage","405"],["dc.bibliographiccitation.volume","176"],["dc.contributor.author","Souchon, R."],["dc.contributor.author","Krege, S."],["dc.contributor.author","Schmoll, Hans-Joachim"],["dc.contributor.author","Albers, P."],["dc.contributor.author","Beyer, J."],["dc.contributor.author","Bokemeyer, Carsten"],["dc.contributor.author","Classen, Joseph"],["dc.contributor.author","Dieckmann, K. P."],["dc.contributor.author","Hartmann, M."],["dc.contributor.author","Heidenreich, A."],["dc.contributor.author","Holtl, W."],["dc.contributor.author","Kliesch, S."],["dc.contributor.author","Kohrmann, K. U."],["dc.contributor.author","Kuczyk, M."],["dc.contributor.author","Schmidberger, Heinz"],["dc.contributor.author","Weinknecht, S."],["dc.contributor.author","Winter, E."],["dc.contributor.author","Wittekind, Christian"],["dc.contributor.author","Bamberg, M."],["dc.date.accessioned","2018-11-07T10:32:47Z"],["dc.date.available","2018-11-07T10:32:47Z"],["dc.date.issued","2000"],["dc.description.abstract","Background: An \"Interdisciplinary Consensus Statement on the Diagnosis and Therapy of Testicular Tumors\" was prepared in 1996 by the \"Interdisciplinary Testicular Tumor Working Group\" (IAH) with input from representatives from diagnostic and therapeutic disciplines of various working groups of the German Cancer Society (Strahlenther Onkol 1997;173:397-406), In 1998 the IAH met again together with the \"Testicular Tumor Working Party\" of the Urooncology Working Group (AUO) and formed the \"German Testicular Cancer Study Group\" (GTCSG). Defined and accepted interdisciplinary standards from the initial meeting were revised based on current scientific developments and clinical results. This cooperating effort increased the quality of the initial recommendations and helped to put the recommendations for diagnosing and treating testicular tumor on a broader scientific basis. Methods: According to the principles of \"evidence-based medicine\" (EBM), the Consensus from 1996 was modified, based on the current level of evidence from the published literature. The methodological process and evaluation criteria used were that of the \"Cochrane Collaboration\". Results: An \"Interdisciplinary Update Consensus Statement\" summarizes and defines the diagnostic and therapeutic standards according to the current scientific practices in testicular cancer. For 21 separate areas scientifically based decision criteria are suggested. For treatment areas where more than one option exist without a consensus being reached for a preferred strategy, such as in seminoma in clinical Stage I or in non-seminoma Stages CS I or CS IIA/B, all acceptable alternative strategies with their respective advantages and disadvantages are presented. This \"Interdisciplinary Update Consensus\" was presented at the 24th National Congress of the German Cancer Society on March 21st and subsequently evaluated and approved by the various German scientific medical societies."],["dc.identifier.doi","10.1007/PL00002347"],["dc.identifier.isi","000089215900002"],["dc.identifier.pmid","11050912"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/44437"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Urban & Vogel"],["dc.relation.issn","0179-7158"],["dc.title","Interdisciplinary consensus on diagnosis and treatment of testicular germ cell tumors. Results of an update conference based on evidence-based medicine (EBM)"],["dc.type","review"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","206"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Cells Tissues Organs"],["dc.bibliographiccitation.lastpage","220"],["dc.bibliographiccitation.volume","196"],["dc.contributor.author","Eildermann, K."],["dc.contributor.author","Aeckerle, Nelia"],["dc.contributor.author","Debowski, Katharina"],["dc.contributor.author","Godmann, M."],["dc.contributor.author","Christiansen, H."],["dc.contributor.author","Heistermann, Michael"],["dc.contributor.author","Schweyer, Stefan"],["dc.contributor.author","Bergmann, M."],["dc.contributor.author","Kliesch, S."],["dc.contributor.author","Gromoll, Joerg"],["dc.contributor.author","Ehmcke, J."],["dc.contributor.author","Schlatt, S."],["dc.contributor.author","Behr, R."],["dc.date.accessioned","2018-11-07T09:14:59Z"],["dc.date.available","2018-11-07T09:14:59Z"],["dc.date.issued","2012"],["dc.description.abstract","SALL4 (sal-like protein 4) is a pluripotency transcription factor, which is highly expressed in embryonic stem (ES) cells and which is essential for mouse preimplantation development. In adult mouse organs, Sall4 mRNA is highly expressed in the testis and ovary, while there is only little or no expression in other organs. There is also a high expression of SALL4 in human testicular germ cell tumors. However, there is as yet no detailed analysis of SALL4 expression during mammalian testicular development. We analyzed SALL4 expression in ES cells, preimplantation embryos, and the developing and adult testis of a nonhuman primate (NHP) species, the common marmoset monkey (Callithrix jacchus). Immunofluorescence revealed SALL4 in the nuclei of marmoset ES cells and preimplantation embryos. Marmoset SALL4 isoform analysis in ES cells and newborn and adult testis by RTPCR and Western blotting showed two different isoforms, SALL4-A and SALL4-B. Immunohistochemistry localized this transcription factor to the nuclei of primordial germ cells and most gonocytes in the prenatal and early postnatal marmoset testis. In the pubertal and adult testis SALL4 was present in undifferentiated spermatogonia. In the developing and adult human and mouse testis SALL4 expression mimicked the pattern in the marmoset. Adult testes from additional NHP species, the treeshrew, the cat and the dog also exhibited SALL4 in undifferentiated spermatogonia, indicating a conserved expression in the mammalian testis. Taking into account the importance of SALL4 for mouse development, we conclude that SALL4 may play an important role during mammalian germ cell development and is involved in the regulation of spermatogonial proliferation in the adult testis. Copyright (C) 2012 S. Karger AG, Basel"],["dc.identifier.doi","10.1159/000335031"],["dc.identifier.isi","000308266400002"],["dc.identifier.pmid","22572102"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9081"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/27561"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Karger"],["dc.relation.issn","1422-6405"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Developmental Expression of the Pluripotency Factor Sal-Like Protein 4 in the Monkey, Human and Mouse Testis: Restriction to Premeiotic Germ Cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","10463"],["dc.bibliographiccitation.issue","33"],["dc.bibliographiccitation.journal","Langmuir"],["dc.bibliographiccitation.lastpage","10474"],["dc.bibliographiccitation.volume","29"],["dc.contributor.author","Schaefer, Edith"],["dc.contributor.author","Kliesch, Torben-Tobias"],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2018-11-07T09:21:12Z"],["dc.date.available","2018-11-07T09:21:12Z"],["dc.date.issued","2013"],["dc.description.abstract","The mechanical response of giant liposomes to compression between two parallel plates is investigated in the context of an artificial actin cortex adjacent to the inner leaflet the bilayer. We found that nonlinear membrane theory neglecting the impact of bending sufficiently describes the mechanical response of liposomes consisting of fluid lipids to compression -whereasthe formation of an actin cortex or the use of gel-phase, lipids generally leads to substantial stiffening of the shell. Giant vesicles are gently adsorbed on glassy surfaces and are compressed with tipless cantilevers using an atomic force microscope. Force-compression curves display a nonlinear response that allows us to determine the membrane tension sigma(0) and the area compressibility modulus K-A by computing the contour of the vesicle as a function of the compression depth. The values for K-A of fluid membranes correspond well to what is known from micropipet-suction experiments and computed from monitoring membrane undulations. The presence of a thick actin shell adjacent to the inner leaflet of the liposome membrane stiffens the system considerably, as mirrored in a significantly higher apparent area compressibility modulus."],["dc.description.sponsorship","DFG [SFB 803]"],["dc.identifier.doi","10.1021/la401969t"],["dc.identifier.isi","000323472000020"],["dc.identifier.pmid","23869855"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/29057"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Chemical Soc"],["dc.relation.issn","0743-7463"],["dc.title","Mechanical Properties of Giant Liposomes Compressed between Two Parallel Plates: Impact of Artificial Actin Shells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","4487"],["dc.bibliographiccitation.issue","22"],["dc.bibliographiccitation.journal","Soft Matter"],["dc.bibliographiccitation.lastpage","4495"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Schaefer, Edith"],["dc.contributor.author","Vache, Marian"],["dc.contributor.author","Kliesch, Torben-Tobias"],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2018-11-07T10:03:13Z"],["dc.date.available","2018-11-07T10:03:13Z"],["dc.date.issued","2015"],["dc.description.abstract","Indentation of giant liposomes with a conical indenter is described by means of a tension-based membrane model. We found that nonlinear membrane theory neglecting the impact of bending sufficiently describes the mechanical response of liposomes to indentation as measured by atomic force microscopy. Giant vesicles are gently adsorbed on glassy surfaces via avidin-biotin linkages and indented centrally using an atomic force microscope equipped with conventional sharp tips mounted on top of an inverted microscope. Force indentation curves display a nonlinear response that allows to extract pre-stress of the bilayer T-0 and the area compressibility modulus K-A by computing the contour of the vesicle at a given force. The values for K-A of fluid membranes correspond well to what is known from micropipet suction experiments and inferred from membrane undulation monitoring. Assembly of actin shells inside the liposome considerably stiffens the vesicles resulting in significantly larger area compressibility modules. The analysis can be easily extended to different indenter geometries with rotational symmetry."],["dc.description.sponsorship","[SFB 803 (B08)]"],["dc.identifier.doi","10.1039/c5sm00191a"],["dc.identifier.isi","000355555100018"],["dc.identifier.pmid","25946988"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12612"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/38409"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Royal Soc Chemistry"],["dc.relation.issn","1744-6848"],["dc.relation.issn","1744-683X"],["dc.rights.access","openAccess"],["dc.title","Mechanical response of adherent giant liposomes to indentation with a conical AFM-tip"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3540"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Nano Letters"],["dc.bibliographiccitation.lastpage","3544"],["dc.bibliographiccitation.volume","16"],["dc.contributor.author","Lambertz, Christina"],["dc.contributor.author","Martos, Ariadna"],["dc.contributor.author","Henkel, Andreas"],["dc.contributor.author","Neiser, Andreas"],["dc.contributor.author","Kliesch, Torben-Tobias"],["dc.contributor.author","Janshoff, Andreas"],["dc.contributor.author","Schwille, Petra"],["dc.contributor.author","Soennichsen, Carsten"],["dc.date.accessioned","2018-11-07T10:13:31Z"],["dc.date.available","2018-11-07T10:13:31Z"],["dc.date.issued","2016"],["dc.description.abstract","We use individual gold nanorods as pointlike detectors for the intrinsic dynamics of an oscillating biological system. We chose the pattern forming MinDE protein system from Escherichia coli (E. coli), a prominent example for self organized chemical oscillations of membrane-associated proteins that are involved in the bacterial cell division process. Similar to surface plasmon resonance (SPR), the gold nanorods report changes in their protein surface coverage without the need for fluorescence labeling, a technique we refer to as NanoSPR Comparing the dynamics for fluorescence labeled and unlabeled proteins, we find a reduction of the oscillation period by about 20%. The absence of photobleaching allows us to investigate Min proteins attaching and detaching from lipid coated gold nanorods with an unprecedented bandwidth of 100 ms time resolution and 1 h observation time. The long observation reveals small changes of the oscillation period over time. Averaging many cycles yields the precise wave profile that exhibits the four phases suggested in previous reports. Unexpected from previous fluorescence-based studies, we found an immobile static protein layer not dissociating during the oscillation cycle. Hence, NanoSPR is an attractive label-free real-time technique for the local investigation of molecular dynamics with high observation bandwidth. It gives access to systems, which cannot be fluorescently labeled, and resolves local dynamics that would average out over the sensor area used in conventional SPR"],["dc.identifier.doi","10.1021/acs.nanolett.6b00507"],["dc.identifier.isi","000377642700019"],["dc.identifier.pmid","27172130"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/40451"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Chemical Soc"],["dc.relation.issn","1530-6992"],["dc.relation.issn","1530-6984"],["dc.title","Single Particle Plasmon Sensors as Label-Free Technique To Monitor MinDE Protein Wave Propagation on Membranes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1068"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","ACS Applied Materials & Interfaces"],["dc.bibliographiccitation.lastpage","1076"],["dc.bibliographiccitation.volume","3"],["dc.contributor.author","Lazzara, Thomas D."],["dc.contributor.author","Kliesch, Torben-Tobias"],["dc.contributor.author","Janshoff, Andreas"],["dc.contributor.author","Steinem, Claudia"],["dc.date.accessioned","2017-09-07T11:44:17Z"],["dc.date.available","2017-09-07T11:44:17Z"],["dc.date.issued","2011"],["dc.description.abstract","Anodic aluminum oxide (AAO) membranes with aligned, cylindrical, nonintersecting pores were selectively fiinctionalized in order to create dual-functionality substrates with different pore-rim and pore-interior surface functionalities, using silane chemistry. We used a two-step process involving an evaporated thin gold film to protect the underlying surface functionality of the pore rims. Subsequent treatment with oxygen plasma of the modified AAO membrane removed the unprotected organic functional groups, i.e., the pore-interior surface. After gold removal, the substrate became optically transparent, and displayed two distinct surface functionalities, one at the pore-rim surface and another at the pore-interior surface. We achieved a selective hydrophobic functionalization with dodecyl-trichlorosilane of either the pore rims or the pore interiors. The deposition of planar lipid membranes on the functionalized areas by addition of small unilamellar vesicles occurred in a predetermined fashion. Small unilamellar vesicles only ruptured upon contact with the hydrophobic substrate regions forming solid supported hybrid bilayers. In addition, pore-rim functionalization with dodecyl-trichlorosilane allowed the formation of pore-spanning hybrid lipid membranes as a result of giant unilamellar vesicle rupture. Confocal laser scanning microscopy was employed to identify the selective spatial localization of the adsorbed fluorescently labeled lipids. The corresponding increase in the AAO refractive index due to lipid adsorption on the hydrophobic regions was monitored by optical waveguide spectroscopy. This simple orthogonal functionalization route is a promising method to control the three-dimensional surface functionality of nanoporous films."],["dc.identifier.doi","10.1021/am101212h"],["dc.identifier.gro","3142745"],["dc.identifier.isi","000289762400021"],["dc.identifier.pmid","21370818"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9425"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/183"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1944-8244"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Orthogonal Functionalization of Nanoporous Substrates: Control of 3D Surface Functionality"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2216"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","2228"],["dc.bibliographiccitation.volume","110"],["dc.contributor.author","Savic, Filip"],["dc.contributor.author","Kliesch, Torben-Tobias"],["dc.contributor.author","Verbeek, Sarah"],["dc.contributor.author","Bao, Chunxiao"],["dc.contributor.author","Thiart, Jan"],["dc.contributor.author","Kros, Alexander"],["dc.contributor.author","Geil, Burkhard"],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2018-11-07T10:14:08Z"],["dc.date.available","2018-11-07T10:14:08Z"],["dc.date.issued","2016"],["dc.description.abstract","The fusion of lipid membranes is a key process in biology. It enables cells and organelles to exchange molecules with their surroundings, which otherwise could not cross the membrane barrier. To study such complex processes we use simplified artificial model systems, i.e., an optical fusion assay based on membrane-coated glass spheres. We present a technique to analyze membrane-membrane interactions in a large ensemble of particles. Detailed information on the geometry of the fusion stalk of fully fused membranes is obtained by studying the diffusional lipid dynamics with fluorescence recovery after photo-bleaching experiments. A small contact zone is a strong obstruction for the particle exchange across the fusion spot. With the aid of computer simulations, fluorescence-recovery-after-photobleaching recovery times of both fused and single-membrane-coated beads allow us to estimate the size of the contact zones between two membrane-coated beads. Minimizing delamination and bending energy leads to minimal angles close to those geometrically allowed."],["dc.description.sponsorship","SFB [803 (B08)]"],["dc.identifier.doi","10.1016/j.bpj.2016.04.026"],["dc.identifier.isi","000376436700012"],["dc.identifier.pmid","27224487"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/40569"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cell Press"],["dc.relation.issn","1542-0086"],["dc.relation.issn","0006-3495"],["dc.title","Geometry of the Contact Zone between Fused Membrane-Coated Beads Mimicking Cell-Cell Fusion"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","77a"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.volume","112"],["dc.contributor.author","Kubsch, Bastian"],["dc.contributor.author","Robinson, Tom"],["dc.contributor.author","Kliesch, Torben-Tobias"],["dc.contributor.author","Janshoff, Andreas"],["dc.contributor.author","Lipowsky, Reinhard"],["dc.contributor.author","Dimova, Rumiana"],["dc.date.accessioned","2020-12-10T14:22:43Z"],["dc.date.available","2020-12-10T14:22:43Z"],["dc.date.issued","2017"],["dc.format.extent","77A"],["dc.identifier.doi","10.1016/j.bpj.2016.11.462"],["dc.identifier.isi","000402328000396"],["dc.identifier.issn","0006-3495"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/71707"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Cell Press"],["dc.publisher.place","Cambridge"],["dc.relation.eventlocation","New Orleans, LA"],["dc.relation.issn","1542-0086"],["dc.relation.issn","0006-3495"],["dc.title","Phase Specific Membrane Fusion with SNARE Mimetics"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","608a"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.volume","114"],["dc.contributor.author","Robinson, Tom"],["dc.contributor.author","Kubsch, Bastian"],["dc.contributor.author","Kliesch, Torben"],["dc.contributor.author","Janshoff, Andreas"],["dc.contributor.author","Lipowsky, Reinhard"],["dc.contributor.author","Dimova, Rumiana"],["dc.date.accessioned","2020-12-10T14:22:44Z"],["dc.date.available","2020-12-10T14:22:44Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1016/j.bpj.2017.11.3323"],["dc.identifier.issn","0006-3495"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/71715"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Spatially Confined Membrane Fusion with SNARE Mimetics"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","19953"],["dc.bibliographiccitation.issue","38"],["dc.bibliographiccitation.journal","The Journal of Biological Chemistry"],["dc.bibliographiccitation.lastpage","19961"],["dc.bibliographiccitation.volume","291"],["dc.contributor.author","Gleisner, Martin"],["dc.contributor.author","Kroppen, Benjamin"],["dc.contributor.author","Fricke, Christian"],["dc.contributor.author","Teske, Nelli"],["dc.contributor.author","Kliesch, Torben-Tobias"],["dc.contributor.author","Janshoff, Andreas"],["dc.contributor.author","Meinecke, Michael"],["dc.contributor.author","Steinem, Claudia"],["dc.date.accessioned","2020-12-10T18:12:56Z"],["dc.date.available","2020-12-10T18:12:56Z"],["dc.date.issued","2016"],["dc.description.abstract","The epsin N-terminal homology domain (ENTH) is a major player in clathrin-mediated endocytosis. To investigate the influence of initial membrane tension on ENTH binding and activity, we established a bilayer system based on adhered giant unilamellar vesicles (GUVs) to be able to control and adjust the membrane tension sigma covering a broad regime. The shape of each individual adhered GUV as well as its adhesion area was monitored by spinning disc confocal laser microscopy. Control of sigma in a range of 0.08-1.02 mN/m was achieved by altering the Mg2+ concentration in solution, which changes the surface adhesion energy per unit area of the GUVs. Specific binding of ENTH to phosphatidylinositol 4,5-bisphosphate leads to a substantial increase in adhesion area of the sessile GUV. At low tension (<0.1 mN/m) binding of ENTH can induce tubular structures, whereas at higher membrane tension the ENTH interaction deflates the sessile GUV and thereby increases the adhesion area. The increase in adhesion area is mainly attributed to a decrease in the area compressibility modulus K-A. We propose that the insertion of the ENTH helix-0 into the membrane is largely responsible for the observed decrease in K-A, which is supported by the observation that the mutant ENTH L6E shows a reduced increase in adhesion area. These results demonstrate that even in the absence of tubule formation, the area compressibility modulus and, as such, the bending rigidity of the membrane is considerably reduced upon ENTH binding. This renders membrane bending and tubule formation energetically less costly."],["dc.identifier.doi","10.1074/jbc.M116.731612"],["dc.identifier.eissn","1083-351X"],["dc.identifier.gro","3141621"],["dc.identifier.isi","000383243100019"],["dc.identifier.issn","0021-9258"],["dc.identifier.pmid","27466364"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/74538"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1083-351X"],["dc.relation.issn","0021-9258"],["dc.relation.orgunit","Institut für Zellbiochemie"],["dc.title","Epsin N-terminal Homology Domain (ENTH) Activity as a Function of Membrane Tension"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]