Flux, Anna Lena

[["dc.bibliographiccitation.artnumber","43"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Diversity"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Flux, Anna Lena"],["dc.contributor.author","Mazanec, Janine"],["dc.contributor.author","Strommenger, Birgit"],["dc.contributor.author","Hummel, Susanne"],["dc.date.accessioned","2019-07-09T11:44:36Z"],["dc.date.available","2019-07-09T11:44:36Z"],["dc.date.issued","2017"],["dc.description.abstract","Staphylococcus aureus is a major pathogen causing osteomyelitis, amongst other diseases, and its methicillin-resistant form (MRSA) in particular poses a huge threat to public health. To increase our knowledge of the origin and evolution of S. aureus, genetic studies of historical microorganisms may be beneficial. Thus, the aim of this study was to investigate whether osteomyelitic skeletal material (autopsy specimens collected from the mid 19th century until the 1920s) is suitable for detecting historical S. aureus DNA sequences. We established a PCR-based analysis system targeting two specific genes of S. aureus (nuc and fib). We successfully amplified the historical S. aureus nuc and fib sequences for six and seven pre-antibiotic, osteomyelitic bone specimens, respectively. These results encourage further investigations of historical S. aureus genomes that may increase our understanding of pathogen evolution in relation to anthropogenically introduced antibiotics."],["dc.identifier.doi","10.3390/d9040043"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14836"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59047"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1424-2818"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","570"],["dc.title","Staphylococcus aureus Sequences from Osteomyelitic Specimens of a Pathological Bone Collection from Pre-Antibiotic Times"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","113"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","BioTechniques"],["dc.bibliographiccitation.lastpage","122"],["dc.bibliographiccitation.volume","73"],["dc.contributor.author","Flux, Anna Lena"],["dc.contributor.author","Schultz, Michael"],["dc.contributor.author","Hummel, Susanne"],["dc.date.accessioned","2022-10-04T10:22:04Z"],["dc.date.available","2022-10-04T10:22:04Z"],["dc.date.issued","2022"],["dc.description.abstract","For microscopic investigation, archaeological bone samples are often embedded in Biodur\n ®\n epoxy resin. This study wants to test whether it is possible to extract DNA suitable for PCR amplification from this sample type. For eight individuals a set of samples – each consisting of a Biodur-embedded femur sample, a native femur sample and a control sample of different anatomical origin – were submitted to organic DNA extraction. The extraction success was tested by autosomal short tandem repeat amplification. Seven out of eight Biodur-embedded femur samples revealed successful amplification results. If Biodur-embedded bone material exists from earlier microscopic investigations, our results encourage the use of this sample type as a source for genetic research."],["dc.description.abstract","Method summary\n \n An organic DNA extraction was performed on archaeological bone samples (femur) embedded in epoxy resin (Biodur\n ®\n ). The DNA extracts were suitable for STR typing of chromosomal DNA."],["dc.identifier.doi","10.2144/btn-2022-0056"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/114582"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-600"],["dc.relation.eissn","1940-9818"],["dc.relation.issn","0736-6205"],["dc.title","Ancient DNA analysis from epoxy resin Biodur\n ®\n -embedded bones"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","S2589004221003874"],["dc.bibliographiccitation.firstpage","102419"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","iScience"],["dc.bibliographiccitation.volume","24"],["dc.contributor.author","Haller, Magdalena"],["dc.contributor.author","Callan, Kimberly"],["dc.contributor.author","Susat, Julian"],["dc.contributor.author","Flux, Anna Lena"],["dc.contributor.author","Immel, Alexander"],["dc.contributor.author","Franke, Andre"],["dc.contributor.author","Herbig, Alexander"],["dc.contributor.author","Krause, Johannes"],["dc.contributor.author","Kupczok, Anne"],["dc.contributor.author","Krause-Kyora, Ben"],["dc.date.accessioned","2021-07-05T15:01:03Z"],["dc.date.available","2021-07-05T15:01:03Z"],["dc.date.issued","2021"],["dc.identifier.doi","10.1016/j.isci.2021.102419"],["dc.identifier.pii","S2589004221003874"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/87977"],["dc.language.iso","en"],["dc.notes.intern","DOI Import DOI-Import GROB-441"],["dc.relation.issn","2589-0042"],["dc.title","Mass burial genomics reveals outbreak of enteric paratyphoid fever in the Late Medieval trade city Lübeck"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Calcified Tissue International"],["dc.contributor.author","Taheri, Shahed"],["dc.contributor.author","Yoshida, Takashi"],["dc.contributor.author","Böker, Kai O."],["dc.contributor.author","Foerster, Robert H."],["dc.contributor.author","Jochim, Lina"],["dc.contributor.author","Flux, Anna Lena"],["dc.contributor.author","Grosskopf, Birgit"],["dc.contributor.author","Lehmann, Wolfgang"],["dc.contributor.author","Schilling, Arndt Friedrich"],["dc.date.accessioned","2021-06-01T09:42:49Z"],["dc.date.available","2021-06-01T09:42:49Z"],["dc.date.issued","2021"],["dc.description.abstract","Abstract The interplay between articular cartilage (AC) and subchondral bone (SB) plays a pivotal role in cartilage homeostasis and functionality. As direct connective pathways between the two are poorly understood, we examined the location-dependent characteristics of the 3D microchannel network within the SB that connects the basal cartilage layer to the bone marrow (i.e. cartilage-bone marrow microchannel connectors; CMMC). 43 measuring points were defined on five human cadaveric femoral heads with no signs of osteoarthritis (OA) (age ≤ 60), and cartilage-bone cylinders with diameters of 2.00 mm were extracted for high-resolution scanning ( n  = 215). The micro-CT data were categorized into three groups (load-bearing region: LBR, n  = 60; non-load-bearing region: NLBR, n  = 60; and the peripheral rim: PR, n  = 95) based on a gait analysis estimation of the joint reaction force (young, healthy cohort with no signs of OA). At the AC-SB interface, the number of CMMC in the LBR was 1.8 times and 2.2 times higher compared to the NLBR, and the PR, respectively. On the other hand, the median Feret size of the CMMC were smallest in the LBR (55.2 µm) and increased in the NLBR (73.5 µm; p  = 0.043) and the PR (89.1 µm; p  = 0.043). AC thickness was positively associated with SB thickness (Pearson's r  = 0.48; p  < 1e-13), CMMC number. ( r  = 0.46; p  < 1e-11), and circularity index ( r  = 0.61; p  < 1e-38). In conclusion, our data suggest that regional differences in the microchannel architecture of SB might reflect regional differences in loading."],["dc.identifier.doi","10.1007/s00223-021-00864-x"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/85362"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.eissn","1432-0827"],["dc.relation.issn","0171-967X"],["dc.title","Investigating the Microchannel Architectures Inside the Subchondral Bone in Relation to Estimated Hip Reaction Forces on the Human Femoral Head"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]