Wurm, Christian Andreas

[["dc.bibliographiccitation.firstpage","337"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","European Journal of Organic Chemistry"],["dc.bibliographiccitation.lastpage","349"],["dc.bibliographiccitation.volume","2015"],["dc.contributor.author","Mitronova, Gyuzel Yu"],["dc.contributor.author","Polyakova, Svetlana"],["dc.contributor.author","Wurm, Christian Andreas"],["dc.contributor.author","Kolmakov, Kirill"],["dc.contributor.author","Wolfram, Thomas"],["dc.contributor.author","Meineke, Dirk N. H."],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","John, Michael"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:44:46Z"],["dc.date.available","2017-09-07T11:44:46Z"],["dc.date.issued","2015"],["dc.description.abstract","Aromatic nucleophilic substitution (SNAr) of fluorine in 9-(3'-carboxy-4',5',6',7'-tetrafluorophenyl) groups of xanthene dyes constitutes a powerful tool in dye design. Thiols and amines regioselectively replace F-6'. This approach enables additional hydrophilic residues or functional groups required for bioconjugation to be introduced. By using this methodology, a \"bright\" and photostable dye for two-color superresolution microscopy was synthesized (with absorption and emission maxima at 604 and 627 nm, respectively). In the case of red-emitting rhodamine dyes with 3'-carboxy-4',5',7'-trifluorophenyl residues, two-dimensional NMR techniques and a chemical transformation were used to prove the precise position of the additional substituent - a carboxylic acid group linked through the S-atom at C-6'. Furthermore, simple H-1 NMR spectra reliably permit the position of the additional carboxy substituent in the 3'-carboxyphenyl ring (at C-5' or C-6') to be established. Information on the exact position of this substituent is significant for the design of molecular probes and for the prediction of the properties of their bioconjugates."],["dc.identifier.doi","10.1002/ejoc.201403269"],["dc.identifier.gro","3141988"],["dc.identifier.isi","000347722900011"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/3301"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1099-0690"],["dc.relation.issn","1434-193X"],["dc.title","Functionalization of the meso-Phenyl Ring of Rhodamine Dyes Through SNAr with Sulfur Nucleophiles: Synthesis, Biophysical Characterizations, and Comprehensive NMR Analysis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","11288"],["dc.bibliographiccitation.issue","36"],["dc.bibliographiccitation.journal","Proceedings of the National Academy of Sciences"],["dc.bibliographiccitation.lastpage","11293"],["dc.bibliographiccitation.volume","112"],["dc.contributor.author","Kukat, Christian"],["dc.contributor.author","Davies, Karen M."],["dc.contributor.author","Wurm, Christian Andreas"],["dc.contributor.author","Spåhr, Henrik"],["dc.contributor.author","Bonekamp, Nina A."],["dc.contributor.author","Kühl, Inge"],["dc.contributor.author","Joos, Friederike"],["dc.contributor.author","Loguercio Polosa, Paola Anna Maria"],["dc.contributor.author","Park, Chan Bae"],["dc.contributor.author","Posse, Viktor"],["dc.contributor.author","Falkenberg, Maria"],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Kühlbrandt, Werner"],["dc.contributor.author","Larsson, Nils-Göran"],["dc.date.accessioned","2017-09-07T11:43:32Z"],["dc.date.available","2017-09-07T11:43:32Z"],["dc.date.issued","2015"],["dc.description.abstract","Mammalian mitochondrial DNA (mtDNA) is packaged by mitochondrial transcription factor A (TFAM) into mitochondrial nucleoids that are of key importance in controlling the transmission and expression of mtDNA. Nucleoid ultrastructure is poorly defined, and therefore we used a combination of biochemistry, super-resolution microscopy, and electron microscopy to show that mitochondrial nucleoids have an irregular ellipsoidal shape and typically contain a single copy of mtDNA. Rotary shadowing electron microscopy revealed that nucleoid formation in vitro is a multistep process initiated by TFAM aggregation and cross-strand binding. Superresolution microscopy of cultivated cells showed that increased mtDNA copy number increases nucleoid numbers without altering their sizes. Electron cryo-tomography visualized nucleoids at high resolution in isolated mammalian mitochondria and confirmed the sizes observed by superresolution microscopy of cell lines. We conclude that the fundamental organizational unit of the mitochondrial nucleoid is a single copy of mtDNA compacted by TFAM, and we suggest a packaging mechanism."],["dc.identifier.doi","10.1073/pnas.1512131112"],["dc.identifier.gro","3141829"],["dc.identifier.isi","000360994900049"],["dc.identifier.pmid","26305956"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1534"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0027-8424"],["dc.title","Cross-strand binding of TFAM to a single mtDNA molecule forms the mitochondrial nucleoid"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","522"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Photochemical & Photobiological Sciences"],["dc.bibliographiccitation.lastpage","532"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Kolmakov, Kirill"],["dc.contributor.author","Wurm, Christian Andreas"],["dc.contributor.author","Sednev, Maksim V."],["dc.contributor.author","Bossi, Mariano L."],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:43:09Z"],["dc.date.available","2017-09-07T11:43:09Z"],["dc.date.issued","2012"],["dc.description.abstract","Caged near-IR emitting fluorescent dyes are in high demand in optical microscopy but up to now were unavailable. We discovered that the combination of a carbopyronine dye core and a photosensitive 2-diazo-1-indanone residue leads to masked near-IR emitting fluorescent dyes. Illumination of these caged dyes with either UV or visible light (lambda < 420 nm) efficiently generates fluorescent compounds with absorption and emission at 635 nm and 660 nm, respectively. A high-yielding synthetic route with attractive possibilities for further dye design is described in detail. Good photostability, high contrast, and a large fluorescence quantum yield after uncaging are the most important features of the new compounds for non-invasive imaging in high-resolution optical microscopy. For use in immunolabelling the caged dyes were decorated with a (hydrophilic) linker and an (activated) carboxyl group."],["dc.identifier.doi","10.1039/c1pp05321c"],["dc.identifier.gro","3142604"],["dc.identifier.isi","000300991600012"],["dc.identifier.pmid","22218703"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/27"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: Bundesministerium fur Bildung und Forschung (BMBF) [513, FKZ 13N11066]"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1474-9092"],["dc.relation.issn","1474-905X"],["dc.title","Masked red-emitting carbopyronine dyes with photosensitive 2-diazo-1-indanone caging group"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2292"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","Molecular Biology of the Cell"],["dc.bibliographiccitation.lastpage","2301"],["dc.bibliographiccitation.volume","23"],["dc.contributor.author","Stoldt, Stefan"],["dc.contributor.author","Wenzel, Dirk"],["dc.contributor.author","Hildenbeutel, Markus"],["dc.contributor.author","Wurm, Christian Andreas"],["dc.contributor.author","Herrmann, Johannes M."],["dc.contributor.author","Jakobs, Stefan"],["dc.date.accessioned","2017-09-07T11:48:51Z"],["dc.date.available","2017-09-07T11:48:51Z"],["dc.date.issued","2012"],["dc.description.abstract","The Oxa1 protein is a well-conserved integral protein of the inner membrane of mitochondria. It mediates the insertion of both mitochondrial-and nuclear-encoded proteins from the matrix into the inner membrane. We investigated the distribution of budding yeast Oxa1 between the two subdomains of the contiguous inner membrane-the cristae membrane (CM) and the inner boundary membrane (IBM)-under different physiological conditions. We found that under fermentable growth conditions, Oxa1 is enriched in the IBM, whereas under nonfermentable (respiratory) growth conditions, it is predominantly localized in the CM. The enrichment of Oxa1 in the CM requires mitochondrial translation; similarly, deletion of the ribosome-binding domain of Oxa1 prevents an enrichment of Oxa1 in the CM. The predominant localization in the IBM under fermentable growth conditions is prevented by inhibiting mitochondrial protein import. Furthermore, overexpression of the nuclear-encoded Oxa1 substrate Mdl1 shifts the distribution of Oxa1 toward the IBM. Apparently, the availability of nuclear- and mitochondrial-encoded substrates influences the inner-membrane distribution of Oxa1. Our findings show that the distribution of Oxa1 within the inner membrane is dynamic and adapts to different physiological needs."],["dc.identifier.doi","10.1091/mbc.E11-06-0538"],["dc.identifier.gro","3142518"],["dc.identifier.isi","000306286700006"],["dc.identifier.pmid","22513091"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9497"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/8878"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1059-1524"],["dc.rights","CC BY-NC-SA 3.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc-sa/3.0"],["dc.title","The inner-mitochondrial distribution of Oxa1 depends on the growth conditions and on the availability of substrates"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","572"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Biology"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Bollmann, Franziska"],["dc.contributor.author","Dohrke, Jan-Niklas"],["dc.contributor.author","Wurm, Christian A."],["dc.contributor.author","Jans, Daniel C."],["dc.contributor.author","Jakobs, Stefan"],["dc.date.accessioned","2021-09-01T06:43:08Z"],["dc.date.available","2021-09-01T06:43:08Z"],["dc.date.issued","2021"],["dc.description.abstract","Mitochondria are highly dynamic organelles that interchange their contents mediated by fission and fusion. However, it has previously been shown that the mitochondria of cultured human epithelial cells exhibit a gradient in the relative abundance of several proteins, with the perinuclear mitochondria generally exhibiting a higher protein abundance than the peripheral mitochondria. The molecular mechanisms that are required for the establishment and the maintenance of such inner-cellular mitochondrial protein abundance gradients are unknown. We verified the existence of inner-cellular gradients in the abundance of clusters of the mitochondrial outer membrane protein Tom20 in the mitochondria of kidney epithelial cells from an African green monkey (Vero cells) using STED nanoscopy and confocal microscopy. We found that the Tom20 gradients are established immediately after cell division and require the presence of microtubules. Furthermore, the gradients are abrogated in hyperfused mitochondrial networks. Our results suggest that inner-cellular protein abundance gradients from the perinuclear to the peripheral mitochondria are established by the trafficking of individual mitochondria to their respective cellular destination."],["dc.description.abstract","Mitochondria are highly dynamic organelles that interchange their contents mediated by fission and fusion. However, it has previously been shown that the mitochondria of cultured human epithelial cells exhibit a gradient in the relative abundance of several proteins, with the perinuclear mitochondria generally exhibiting a higher protein abundance than the peripheral mitochondria. The molecular mechanisms that are required for the establishment and the maintenance of such inner-cellular mitochondrial protein abundance gradients are unknown. We verified the existence of inner-cellular gradients in the abundance of clusters of the mitochondrial outer membrane protein Tom20 in the mitochondria of kidney epithelial cells from an African green monkey (Vero cells) using STED nanoscopy and confocal microscopy. We found that the Tom20 gradients are established immediately after cell division and require the presence of microtubules. Furthermore, the gradients are abrogated in hyperfused mitochondrial networks. Our results suggest that inner-cellular protein abundance gradients from the perinuclear to the peripheral mitochondria are established by the trafficking of individual mitochondria to their respective cellular destination."],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft"],["dc.description.sponsorship","European Research Council"],["dc.identifier.doi","10.3390/biology10070572"],["dc.identifier.pii","biology10070572"],["dc.identifier.pmid","34201436"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/89225"],["dc.identifier.url","https://sfb1190.med.uni-goettingen.de/production/literature/publications/146"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-455"],["dc.relation","SFB 1190: Transportmaschinen und Kontaktstellen zellulärer Kompartimente"],["dc.relation","SFB 1190 | P01: Untersuchung der Unterschiede in der Zusammensetzung, Funktion und Position von individuellen MICOS Komplexen in einzelnen Säugerzellen"],["dc.relation.eissn","2079-7737"],["dc.relation.workinggroup","RG Jakobs (Structure and Dynamics of Mitochondria)"],["dc.rights","https://creativecommons.org/licenses/by/4.0/"],["dc.title","Mitochondrial Protein Abundance Gradients Require the Distribution of Separated Mitochondria"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","185"],["dc.bibliographiccitation.lastpage","199"],["dc.bibliographiccitation.seriesnr","591"],["dc.contributor.author","Wurm, Christian Andreas"],["dc.contributor.author","Neumann, Daniel"],["dc.contributor.author","Schmidt, Christoph"],["dc.contributor.author","Egner, Alexander"],["dc.contributor.author","Jakobs, Stefan"],["dc.date.accessioned","2017-09-07T11:53:08Z"],["dc.date.available","2017-09-07T11:53:08Z"],["dc.date.issued","2009"],["dc.identifier.doi","10.1007/978-1-60761-404-3_11"],["dc.identifier.gro","3145045"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2738"],["dc.language.iso","en"],["dc.notes.intern","Crossref Import"],["dc.notes.status","final"],["dc.publisher","Springer Nature"],["dc.relation.crisseries","Methods in Molecular Biology"],["dc.relation.isbn","978-1-60761-403-6"],["dc.relation.ispartof","Live Cell Imaging: Methods and Protocols"],["dc.relation.ispartofseries","Methods in Molecular Biology; 591"],["dc.relation.issn","1064-3745"],["dc.title","Sample Preparation for STED Microscopy"],["dc.type","book_chapter"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","73"],["dc.bibliographiccitation.lastpage","88"],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Andresen, Martin"],["dc.contributor.author","Wurm, Christian A."],["dc.contributor.editor","Miller, Lawrence W."],["dc.date.accessioned","2021-12-08T12:28:57Z"],["dc.date.available","2021-12-08T12:28:57Z"],["dc.date.issued","2008"],["dc.identifier.doi","10.1002/9783527623099.ch4"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/95899"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-476"],["dc.notes.status","final"],["dc.publisher","Wiley-VCH Verlag GmbH & Co. KGaA"],["dc.publisher.place","Weinheim, Germany"],["dc.relation.eisbn","9783527623099"],["dc.relation.isbn","9783527315666"],["dc.relation.ispartof","Probes and tags to study biomolecular function: for proteins, RNA, and membranes"],["dc.title","“FlAsH” Protein Labeling"],["dc.type","book_chapter"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","L67"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","L69"],["dc.bibliographiccitation.volume","92"],["dc.contributor.author","Donnert, Gerald"],["dc.contributor.author","Keller, Jan"],["dc.contributor.author","Wurm, Christian Andreas"],["dc.contributor.author","Rizzoli, Silvio"],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Schoenle, Andreas"],["dc.contributor.author","Jahn, Reinhard"],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:49:49Z"],["dc.date.available","2017-09-07T11:49:49Z"],["dc.date.issued","2007"],["dc.description.abstract","We demonstrate two-color fluorescence microscopy with nanoscale spatial resolution by applying stimulated emission depletion on fluorophores differing in their absorption and emission spectra. Green- and red-emitting fluorophores are selectively excited and quenched using dedicated beam pairs. The stimulated emission depletion beams deliver a lateral resolution of < 30 nm and 65 nm for the green and the red color channel, respectively. The similar to 5 nm alignment accuracy of the two images establishes the precision with which differently labeled proteins are correlated in space. Colocalized nanoscopy is demonstrated with endosomal protein patterns and by resolving nanoclusters of a mitochondrial outer membrane protein, Tom20, in relation with the F(1)F(0)ATP synthase. The joint improvement of resolution and colocalization demonstrates the emerging potential of far-field fluorescence nanoscopy to study the spatial organization of macromolecules in cells."],["dc.identifier.doi","10.1529/biophysj.107.104497"],["dc.identifier.gro","3143514"],["dc.identifier.isi","000245164000003"],["dc.identifier.pmid","17307826"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1037"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0006-3495"],["dc.title","Two-color far-field fluorescence nanoscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3598"],["dc.bibliographiccitation.issue","20"],["dc.bibliographiccitation.journal","Angewandte Chemie"],["dc.bibliographiccitation.lastpage","3602"],["dc.bibliographiccitation.volume","122"],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Wurm, Christian Andreas"],["dc.contributor.author","Boyarskiy, Vadim P."],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:53:02Z"],["dc.date.available","2017-09-07T11:53:02Z"],["dc.date.issued","2010"],["dc.identifier.doi","10.1002/ange.201000150"],["dc.identifier.gro","3145021"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2711"],["dc.language.iso","de"],["dc.notes.intern","Crossref Import"],["dc.notes.status","final"],["dc.relation.issn","0044-8249"],["dc.title","Rhodamine NN: eine neue Klasse maskierter Fluoreszenzfarbstoffe"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","943"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Nature Methods"],["dc.bibliographiccitation.lastpage","945"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Fölling, Jonas"],["dc.contributor.author","Bossi, Mariano"],["dc.contributor.author","Bock, Hannes"],["dc.contributor.author","Medda, Rebecca"],["dc.contributor.author","Wurm, Christian A."],["dc.contributor.author","Hein, Birka"],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2017-09-07T11:48:10Z"],["dc.date.available","2017-09-07T11:48:10Z"],["dc.date.issued","2008"],["dc.description.abstract","We introduce far-field fluorescence nanoscopy with ordinary fluorophores based on switching the majority of them to a metastable dark state, such as the triplet, and calculating the position of those left or those that spontaneously returned to the ground state. Continuous widefield illumination by a single laser and a continuously operating camera yielded dual-color images of rhodamine-and fluorescent protein-labeled (living) samples, proving a simple yet powerful super-resolution approach."],["dc.identifier.doi","10.1038/nmeth.1257"],["dc.identifier.gro","3143218"],["dc.identifier.isi","000260532500012"],["dc.identifier.pmid","18794861"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/708"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1548-7091"],["dc.title","Fluorescence nanoscopy by ground-state depletion and single-molecule return"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]