Czesnik, Dirk

[["dc.bibliographiccitation.firstpage","11193"],["dc.bibliographiccitation.issue","21"],["dc.bibliographiccitation.journal","International Journal of Environmental Research and Public Health"],["dc.bibliographiccitation.volume","18"],["dc.contributor.author","Veiz, Elisabeth"],["dc.contributor.author","Kieslich, Susann-Kristin"],["dc.contributor.author","Staab, Julia"],["dc.contributor.author","Czesnik, Dirk"],["dc.contributor.author","Herrmann-Lingen, Christoph"],["dc.contributor.author","Meyer, Thomas"],["dc.date.accessioned","2021-12-01T09:22:50Z"],["dc.date.available","2021-12-01T09:22:50Z"],["dc.date.issued","2021"],["dc.description.abstract","This paper presents data from a transcutaneous vagus nerve stimulation experiment that point towards a blunted cardiac baroreceptor sensitivity (cBRS) in young males compared to females during electrical stimulation of the forearm and a rhythmic breathing task. Continuous electrocardiography, impedance cardiography and continuous blood-pressure recordings were assessed in a sex-matched cohort of twenty young healthy subjects. Electrical stimulation of the median nerve was conducted by using a threshold-tracking method combined with two rhythmic breathing tasks (0.1 and 0.2 Hz) before, during and after active or sham transcutaneous vagus nerve stimulation. Autonomic and hemodynamic parameters were calculated, and differences were analyzed by using linear mixed models and post hoc F-tests. None of the autonomic and hemodynamic parameters differed between the sham and active conditions. However, compared to females, male participants had an overall lower total cBRS independent of stimulation condition during nerve stimulation (females: 14.96 ± 5.67 ms/mmHg, males: 11.89 ± 3.24 ms/mmHg, p = 0.031) and rhythmic breathing at 0.2 Hz (females: 21.49 ± 8.47 ms/mmHg, males: 15.12 ± 5.70 ms/mmHg, p = 0.004). Whereas vagus nerve stimulation at the left inner tragus did not affect the efferent vagal control of the heart, we found similar patterns of baroreceptor sensitivity activation over the stimulation period in both sexes, which, however, significantly differed in their magnitude, with females showing an overall higher cBRS."],["dc.description.abstract","This paper presents data from a transcutaneous vagus nerve stimulation experiment that point towards a blunted cardiac baroreceptor sensitivity (cBRS) in young males compared to females during electrical stimulation of the forearm and a rhythmic breathing task. Continuous electrocardiography, impedance cardiography and continuous blood-pressure recordings were assessed in a sex-matched cohort of twenty young healthy subjects. Electrical stimulation of the median nerve was conducted by using a threshold-tracking method combined with two rhythmic breathing tasks (0.1 and 0.2 Hz) before, during and after active or sham transcutaneous vagus nerve stimulation. Autonomic and hemodynamic parameters were calculated, and differences were analyzed by using linear mixed models and post hoc F-tests. None of the autonomic and hemodynamic parameters differed between the sham and active conditions. However, compared to females, male participants had an overall lower total cBRS independent of stimulation condition during nerve stimulation (females: 14.96 ± 5.67 ms/mmHg, males: 11.89 ± 3.24 ms/mmHg, p = 0.031) and rhythmic breathing at 0.2 Hz (females: 21.49 ± 8.47 ms/mmHg, males: 15.12 ± 5.70 ms/mmHg, p = 0.004). Whereas vagus nerve stimulation at the left inner tragus did not affect the efferent vagal control of the heart, we found similar patterns of baroreceptor sensitivity activation over the stimulation period in both sexes, which, however, significantly differed in their magnitude, with females showing an overall higher cBRS."],["dc.identifier.doi","10.3390/ijerph182111193"],["dc.identifier.pii","ijerph182111193"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/94492"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-478"],["dc.publisher","MDPI"],["dc.relation.eissn","1660-4601"],["dc.rights","Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/)."],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0/"],["dc.title","Men Show Reduced Cardiac Baroreceptor Sensitivity during Modestly Painful Electrical Stimulation of the Forearm: Exploratory Results from a Sham-Controlled Crossover Vagus Nerve Stimulation Study"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","925"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","European Journal of Neuroscience"],["dc.bibliographiccitation.lastpage","934"],["dc.bibliographiccitation.volume","26"],["dc.contributor.author","Manzini, Ivan"],["dc.contributor.author","Heermann, Stephan"],["dc.contributor.author","Czesnik, Dirk"],["dc.contributor.author","Brase, Christoph"],["dc.contributor.author","Schild, Detlev"],["dc.contributor.author","Roessler, Wolfgang"],["dc.date.accessioned","2018-11-07T10:59:55Z"],["dc.date.available","2018-11-07T10:59:55Z"],["dc.date.issued","2007"],["dc.description.abstract","The sensory input layer in the olfactory bulb (OB) is typically organized into spheroidal aggregates of dense neuropil called glomeruli. This characteristic compartmentalization of the synaptic neuropil is a typical feature of primary olfactory centres in vertebrates and most advanced invertebrates. In the present work we mapped the location of presynaptic sites in glomeruli across the OB using antibodies to presynaptic vesicle proteins and presynaptic membrane proteins in combination with confocal microscopy. In addition the responses of glomeruli upon mucosal application of amino acid-odorants and forskolin were monitored using functional calcium imaging. We first describe the spatial distribution of glomeruli across the main olfactory bulb (MOB) in premetamorphic Xenopus laevis. Second, we show that the heterogeneous organization of glomeruli along the dorsoventral and mediolateral axes of the MOB is associated with a differential distribution of synaptic vesicle proteins. While antibodies to synaptophysin, syntaxin and SNAP-25 uniformly labelled glomeruli in the whole MOB, intense synaptotagmin staining was present only in glomeruli in the lateral, and to a lesser extent in the intermediate, part of the OB. Interestingly, amino acid-responsive glomeruli were always located in the lateral part of the OB, and glomeruli activated by mucosal forskolin application were exclusively located in the medial part of the OB. This correlation between odour mapping and presynaptic protein distribution is an additional hint on the existence of different subsystems within the main olfactory system in larval Xenopus laevis."],["dc.identifier.doi","10.1111/j.1460-9568.2007.05731.x"],["dc.identifier.isi","000248963400013"],["dc.identifier.pmid","17666078"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7761"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/50808"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell Publishing, Inc"],["dc.relation.issn","0953-816X"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Presynaptic protein distribution and odour mapping in glomeruli of the olfactory bulb of Xenopus laevis tadpoles"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","129"],["dc.bibliographiccitation.issue","1-2"],["dc.bibliographiccitation.journal","Journal of Neuroscience Methods"],["dc.bibliographiccitation.lastpage","135"],["dc.bibliographiccitation.volume","99"],["dc.contributor.author","Peters, F."],["dc.contributor.author","Gennerich, A."],["dc.contributor.author","Czesnik, D."],["dc.contributor.author","Schild, Detlev"],["dc.date.accessioned","2018-11-07T10:43:42Z"],["dc.date.available","2018-11-07T10:43:42Z"],["dc.date.issued","2000"],["dc.description.abstract","We implemented a simple feedback system that modifies the conventional current clamp mode of a patch clamp amplifier so that transient potentials, such as action potentials and synaptic potentials, can be measured as in the usual current clamp, while the average membrane potential is kept constant at a value chosen by the user. The circuit thus works like the current clamp for high frequency signals and like a voltage clamp for low frequency signals. We delineate its transfer properties and give application examples. (C) 2000 Elsevier Science B.V. All rights reserved."],["dc.identifier.doi","10.1016/S0165-0270(00)00223-5"],["dc.identifier.isi","000088444700016"],["dc.identifier.pmid","10936652"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9904"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/47114"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Bv"],["dc.relation.issn","0165-0270"],["dc.title","Low frequency voltage clamp: recording of voltage transients at constant average command voltage"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","8965"],["dc.bibliographiccitation.issue","26"],["dc.bibliographiccitation.journal","Journal of Neuroscience"],["dc.bibliographiccitation.lastpage","8973"],["dc.bibliographiccitation.volume","30"],["dc.contributor.author","Breunig, Esther"],["dc.contributor.author","Manzini, Ivan"],["dc.contributor.author","Piscitelli, Fabiana"],["dc.contributor.author","Gutermann, Benjamin"],["dc.contributor.author","Di Marzo, Vincenzo"],["dc.contributor.author","Schild, Detlev"],["dc.contributor.author","Czesnik, Dirk"],["dc.date.accessioned","2018-11-07T08:42:06Z"],["dc.date.available","2018-11-07T08:42:06Z"],["dc.date.issued","2010"],["dc.description.abstract","Cannabinoids modulate the activity of many neuronal cells, among them sensory neurons in the olfactory epithelium. Here we show that the endocannabinoid 2-arachidonoyl-glycerol (2-AG) is synthesized in both olfactory receptor neurons and glia-like sustentacular cells in larval Xenopus laevis. Its production in the latter depends on the hunger state of the animal. The essential effect of 2-AG in olfactory receptor neurons is the control of odorant detection thresholds via cannabinoid CB(1) receptor activation. Hunger renders olfactory neurons more sensitive. Endocannabinoid modulation in the nose may therefore substantially influence food-seeking behavior."],["dc.identifier.doi","10.1523/JNEUROSCI.4030-09.2010"],["dc.identifier.isi","000279581500028"],["dc.identifier.pmid","20592217"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/6313"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/19629"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Soc Neuroscience"],["dc.relation.issn","0270-6474"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","The Endocannabinoid 2-Arachidonoyl-Glycerol Controls Odor Sensitivity in Larvae of Xenopus laevis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1093"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","The European journal of neuroscience"],["dc.bibliographiccitation.lastpage","100"],["dc.bibliographiccitation.volume","13"],["dc.contributor.author","Czesnik, D."],["dc.contributor.author","Nezlin, L."],["dc.contributor.author","Rabba, J."],["dc.contributor.author","Müller, B."],["dc.contributor.author","Schild, D."],["dc.date.accessioned","2019-07-10T08:14:11Z"],["dc.date.available","2019-07-10T08:14:11Z"],["dc.date.issued","2001-03-01"],["dc.description.abstract","Norepinephrine (NE) has various modulatory roles in both the peripheral and the central nervous systems. Here we investigate the function of the locus coeruleus efferent fibres in the olfactory bulb of Xenopus laevis tadpoles. In order to distinguish unambiguously between mitral cells and granule cells of the main olfactory bulb and the accessory olfactory bulb, we used a slice preparation. The two neuron types were distinguished on the basis of their location in the slice, their typical branching pattern and by electrophysiological criteria. At NE concentrations lower than 5 microM there was only one effect of NE upon voltage-gated conductances; NE blocked a high-voltage-activated Ca(2+)-current in mitral cells of both the main and the accessory olfactory bulbs. No such effect was observed in granule cells. The effect of NE upon mitral cell Ca(2+)-currents was mimicked by the alpha(2)-receptor agonists clonidine and alpha-methyl-NE. As a second effect, NE or clonidine blocked spontaneous synaptic activity in granule cells of both the main and the accessory olfactory bulbs. NE or clonidine also blocked the spontaneous synaptic activity in mitral cells of either olfactory bulb. The amplitude of glutamate-induced currents in granule cells was modulated neither by clonidine nor by alpha-methyl-NE. Taken together, the main effect of the noradrenergic, presynaptic, alpha(2)-receptor-mediated block of Ca(2)+-currents in mitral cells appeared to be a wide-spread disinhibition of mitral cells in the accessory olfactory bulb as well as in the main olfactory bulb."],["dc.identifier.fs","2568"],["dc.identifier.pmid","11285006"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9909"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/61458"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","0953-816X"],["dc.relation.orgunit","Universitätsmedizin Göttingen"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.mesh","Animals"],["dc.subject.mesh","Calcium Channel Blockers"],["dc.subject.mesh","Calcium Channels"],["dc.subject.mesh","Clonidine"],["dc.subject.mesh","Electric Conductivity"],["dc.subject.mesh","Larva"],["dc.subject.mesh","Neurons"],["dc.subject.mesh","Norepinephrine"],["dc.subject.mesh","Olfactory Bulb"],["dc.subject.mesh","Synapses"],["dc.subject.mesh","Synaptic Transmission"],["dc.subject.mesh","Xenopus laevis"],["dc.title","Noradrenergic modulation of calcium currents and synaptic transmission in the olfactory bulb of Xenopus laevis tadpoles."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2394"],["dc.bibliographiccitation.journal","Brain"],["dc.bibliographiccitation.lastpage","2403"],["dc.bibliographiccitation.volume","129"],["dc.contributor.author","Djukic, Marija"],["dc.contributor.author","Mildner, Alexander"],["dc.contributor.author","Schmidt, Hauke"],["dc.contributor.author","Czesnik, Dirk"],["dc.contributor.author","Brueck, Wolfgang"],["dc.contributor.author","Priller, Josef"],["dc.contributor.author","Nau, Roland"],["dc.contributor.author","Prinz, Marco R."],["dc.date.accessioned","2018-11-07T09:17:35Z"],["dc.date.available","2018-11-07T09:17:35Z"],["dc.date.issued","2006"],["dc.description.abstract","Previous studies have demonstrated a potential role of brain endogenous microglia and meningeal macrophages in inflammation and brain injury during bacterial meningitis. However, the contribution of previously engrafted monocytes and microglia to this process is still unknown. We therefore used genetically labelled bone marrow-derived cells from transgenic mice expressing the green fluorescent protein (GFP) under the chicken beta-actin promoter to deliver fluorescently labelled monocytes to the diseased brain. Approximately 24 hours after Streptococcus pneumoniae infection, GFP-expressing parenchymal microglia changed their morphology to an activated phenotype and upregulated major histocompatibility complex class II molecules. Bacterial meningitis increased the engraftment of GFP(+) monocytes and their differentiation to microglia during the post-inflammatory period, but not during acute meningitis. Importantly, these newly recruited monocytes became an integral part of the pool of parenchymal microglia and contributed to the clearance of damaged tissue by increased lysosomal activity and close location to apoptotic cells. Thus, circulating cells entering the brain such as monocytes/macrophages might provide a potential cellular target for the treatment of the tissue damage following meningitis via peripheral cell therapy."],["dc.identifier.doi","10.1093/brain/awl206"],["dc.identifier.isi","000240679700015"],["dc.identifier.pmid","16891321"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7750"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/28202"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press"],["dc.relation.issn","0006-8950"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Circulating monocytes engraft in the brain, differentiate into microglia and contribute to the pathology following meningitis in mice"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","888"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Brain Sciences"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Antal, Andrea"],["dc.contributor.author","Bischoff, Rebecca"],["dc.contributor.author","Stephani, Caspar"],["dc.contributor.author","Czesnik, Dirk"],["dc.contributor.author","Klinker, Florian"],["dc.contributor.author","Timäus, Charles"],["dc.contributor.author","Chaieb, Leila"],["dc.contributor.author","Paulus, Walter"],["dc.date.accessioned","2021-04-14T08:31:09Z"],["dc.date.available","2021-04-14T08:31:09Z"],["dc.date.issued","2020"],["dc.description.sponsorship","Migraine Research Foundation"],["dc.identifier.doi","10.3390/brainsci10110888"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17667"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/83498"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.notes.intern","Merged from goescholar"],["dc.publisher","MDPI"],["dc.relation.eissn","2076-3425"],["dc.rights","https://creativecommons.org/licenses/by/4.0/"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Low Intensity, Transcranial, Alternating Current Stimulation Reduces Migraine Attack Burden in a Home Application Set-Up: A Double-Blinded, Randomized Feasibility Study"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","28041"],["dc.bibliographiccitation.issue","32"],["dc.bibliographiccitation.journal","Journal of Biological Chemistry"],["dc.bibliographiccitation.lastpage","28048"],["dc.bibliographiccitation.volume","286"],["dc.contributor.author","Breunig, Esther"],["dc.contributor.author","Kludt, Eugen"],["dc.contributor.author","Czesnik, Dirk"],["dc.contributor.author","Schild, Detlev"],["dc.date.accessioned","2018-11-07T08:53:16Z"],["dc.date.available","2018-11-07T08:53:16Z"],["dc.date.issued","2011"],["dc.description.abstract","Many olfactory receptor neurons use a cAMP-dependent transduction mechanism to transduce odorants into depolarizations. This signaling cascade is characterized by a sequence of two currents: a cation current through cyclic nucleotide-gated channels followed by a chloride current through calcium-activated chloride channels. To date, it is not possible to interfere with these generator channels under physiological conditions with potent and specific blockers. In this study we identified the styryl dye FM1-43 as a potent blocker of native olfactory cyclic nucleotide-gated channels. Furthermore, we characterized this substance to stain olfactory receptor neurons that are endowed with cAMP-dependent transduction. This allows optical differentiation and pharmacological interference with olfactory receptor neurons at the level of the signal transduction."],["dc.identifier.doi","10.1074/jbc.M111.233890"],["dc.identifier.isi","000293557800022"],["dc.identifier.pmid","21646359"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7624"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/22365"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Soc Biochemistry Molecular Biology Inc"],["dc.relation.issn","0021-9258"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","The Styryl Dye FM1-43 Suppresses Odorant Responses in a Subset of Olfactory Neurons by Blocking Cyclic Nucleotide-gated (CNG) Channels"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","9"],["dc.bibliographiccitation.journal","Cell Communication and Signaling"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Engels, Niklas"],["dc.contributor.author","Yigit, Goekhan"],["dc.contributor.author","Emmerich, Christoph H."],["dc.contributor.author","Czesnik, Dirk"],["dc.contributor.author","Schild, Detlev"],["dc.contributor.author","Wienands, Juergen"],["dc.date.accessioned","2018-11-07T09:11:16Z"],["dc.date.available","2018-11-07T09:11:16Z"],["dc.date.issued","2012"],["dc.description.abstract","Background: The latent membrane protein (LMP) 2A of Epstein-Barr virus (EBV) is expressed during different latency stages of EBV-infected B cells in which it triggers activation of cytoplasmic protein tyrosine kinases. Early studies revealed that an immunoreceptor tyrosine-based activation motif (ITAM) in the cytoplasmic N-terminus of LMP2A can trigger a transient increase of the cytosolic Ca2+ concentration similar to that observed in antigen-activated B cells when expressed as a chimeric transmembrane receptor. Even so, LMP2A was subsequently ascribed an inhibitory rather than an activating function because its expression seemed to partially inhibit B cell antigen receptor (BCR) signaling in EBV-transformed B cell lines. However, the analysis of LMP2A signaling has been hampered by the lack of cellular model systems in which LMP2A can be studied without the influence of other EBV-encoded factors. Results: We have reanalyzed LMP2A signaling using B cells in which LMP2A is expressed in an inducible manner in the absence of any other EBV signaling protein. This allowed us for the first time to monitor LMP2A signaling in statu nascendi as it occurs during the EBV life cycle in vivo. We show that mere expression of LMP2A not only stimulated protein tyrosine kinases but also induced phospholipase C-gamma 2-mediated Ca2+ oscillations followed by activation of the extracellular signal-regulated kinase (Erk) mitogen-activated protein kinase pathway and induction of the lytic EBV gene bzlf1. Furthermore, expression of the constitutively phosphorylated LMP2A ITAM modulated rather than inhibited BCR-induced Ca2+ mobilization. Conclusion: Our data establish that LMP2A expression has a function beyond the putative inhibition of the BCR by generating a ligand-independent cellular activation signal that may provide a molecular switch for different EBV life cycle stages and most probably contributes to EBV-associated lymphoproliferative disorders."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2012"],["dc.identifier.doi","10.1186/1478-811X-10-9"],["dc.identifier.isi","000304033500001"],["dc.identifier.pmid","22472181"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7606"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/26681"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1478-811X"],["dc.rights","CC BY 2.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.0"],["dc.title","Epstein-Barr virus LMP2A signaling in statu nascendi mimics a B cell antigen receptor-like activation signal"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","119"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","European Journal of Neuroscience"],["dc.bibliographiccitation.lastpage","128"],["dc.bibliographiccitation.volume","23"],["dc.contributor.author","Czesnik, D."],["dc.contributor.author","Kuduz, Josko"],["dc.contributor.author","Schild, Detlev"],["dc.contributor.author","Manzini, Ivan"],["dc.date.accessioned","2018-11-07T10:33:50Z"],["dc.date.available","2018-11-07T10:33:50Z"],["dc.date.issued","2006"],["dc.description.abstract","Nucleotides and amino acids are acknowledged categories of water-borne olfactory stimuli. In previous studies it has been shown that larvae of Xenopus laevis are able to sense amino acids. Here we report on the effect of ATP in the olfactory epithelium (OE) of Xenopus laevis tadpoles. First, ATP activates a subpopulation of cells in the OE. The ATP-sensitive subset of cells is almost perfectly disjoint from the subset of amino acid-activated cells. Both responses are not mediated by the well-described cAMP transduction pathway as the two subpopulations of cells do not overlap with a third, forskolin-activated subpopulation. We further show that, in contrast to amino acids, which act exclusively as olfactory stimuli, ATP appears to feature a second role. Surprisingly it activated a large number of sustentacular supporting cells (SCs) and, to a much lower extent, olfactory receptor neurons. The cells of the amino acid- and ATP-responding subsets featured differences in shape, size and position in the OE. The latencies to activation upon stimulus application differed markedly in these subsets. To obtain these results two technical points were important. We used a novel dextran-tetramethylrhodamine-backfilled slice preparation of the OE and we found out that an antibody to calnexin, a known molecular chaperone, also labels SCs. Our findings thus show a strong effect of ATP in the OE and we discuss some of the possible physiological functions of nucleotides in the OE."],["dc.identifier.doi","10.1111/j.1460-9568.2005.04533.x"],["dc.identifier.isi","000235052400013"],["dc.identifier.pmid","16420422"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7748"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/44713"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Blackwell Publishing"],["dc.relation.issn","0953-816X"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","ATP activates both receptor and sustentacular supporting cells in the olfactory epithelium of Xenopus laevis tadpoles"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]