Köster, Sarah

[["dc.bibliographiccitation.artnumber","088102"],["dc.bibliographiccitation.firstpage","3"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Physical Review Letters"],["dc.bibliographiccitation.lastpage","7"],["dc.bibliographiccitation.volume","112"],["dc.contributor.author","Weinhausen, Britta"],["dc.contributor.author","Saldanha, Oliva"],["dc.contributor.author","Wilke, Robin N."],["dc.contributor.author","Dammann, Christian"],["dc.contributor.author","Priebe, Marius"],["dc.contributor.author","Burghammer, Manfred"],["dc.contributor.author","Sprung, Michael"],["dc.contributor.author","Köster, Sarah"],["dc.date.accessioned","2017-09-07T11:46:29Z"],["dc.date.available","2017-09-07T11:46:29Z"],["dc.date.issued","2014"],["dc.description.abstract","High-resolution x-ray imaging techniques offer a variety of possibilities for studying the nanoscale structure of biological cells. A challenging task remains the study of cells by x rays in their natural, aqueous environment. Here, we overcome this limitation by presenting scanning x-ray diffraction measurements with beam sizes in the range of a few hundred nm on living and fixed-hydrated eukaryotic cells in microfluidic devices which mimic a native environment. The direct comparison between fixed-hydrated and living cells shows distinct differences in the scattering signal, pointing to structural changes on the order of 30 to 50 nm."],["dc.identifier.doi","10.1103/PhysRevLett.112.088102"],["dc.identifier.gro","3142182"],["dc.identifier.isi","000331957600012"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/5443"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1079-7114"],["dc.relation.issn","0031-9007"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Köster (Cellular Biophysics)"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","x-ray scattering"],["dc.subject.gro","cellular biophysics"],["dc.subject.gro","microfluidics"],["dc.title","Scanning X-Ray Nanodiffraction on Living Eukaryotic Cells in Microfluidic Environments"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3553"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","ACS Nano"],["dc.bibliographiccitation.lastpage","3561"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Hemonnot, Clement Y. J."],["dc.contributor.author","Reinhardt, Juliane"],["dc.contributor.author","Saldanha, Oliva"],["dc.contributor.author","Patommel, Jens"],["dc.contributor.author","Graceffa, Rita"],["dc.contributor.author","Weinhausen, Britta"],["dc.contributor.author","Burghammer, Manfred"],["dc.contributor.author","Schroer, Christian G."],["dc.contributor.author","Köster, Sarah"],["dc.date.accessioned","2017-09-07T11:54:36Z"],["dc.date.available","2017-09-07T11:54:36Z"],["dc.date.issued","2016"],["dc.description.abstract","In recent years, X-ray imaging of biological cells has emerged as a complementary alternative to fluorescence and electron microscopy. Different techniques were established and successfully applied to macromolecular assemblies and structures in cells. However, while the resolution is reaching the nanometer scale, the dose is increasing. It is essential to develop strategies to overcome or reduce radiation damage. Here we approach this intrinsic problem by combing two different X-ray techniques, namely ptychography and nanodiffraction, in one experiment and on the same sample. We acquire low dose ptychography overview images of whole cells at a resolution of 65 nm. We subsequently record high-resolution nanodiffraction data from regions of interest. By comparing images from the two modalities, we can exclude strong effects of radiation damage on the specimen. From the diffraction data we retrieve quantitative structural information from intracellular bundles of keratin intermediate filaments such as a filament radius of 5 nm, hexagonal geometric arrangement with an interfilament distance of 14 nm and bundle diameters on the order of 70 nm. Thus, we present an appealing combined approach to answer a broad range of questions in soft matter physics, biophysics and biology."],["dc.identifier.doi","10.1021/acsnano.5b07871"],["dc.identifier.gro","3141720"],["dc.identifier.isi","000372855400058"],["dc.identifier.pmid","26905642"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/324"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1936-086X"],["dc.relation.issn","1936-0851"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Köster (Cellular Biophysics)"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","x-ray scattering"],["dc.subject.gro","cytoskeleton"],["dc.subject.gro","cellular biophysics"],["dc.title","X-rays Reveal the Internal Structure of Keratin Bundles in Whole Cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","10661"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","ACS Nano"],["dc.bibliographiccitation.lastpage","10670"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Hémonnot, Clément Y. J."],["dc.contributor.author","Ranke, Christiane"],["dc.contributor.author","Saldanha, Oliva"],["dc.contributor.author","Graceffa, Rita"],["dc.contributor.author","Hagemann, Johannes"],["dc.contributor.author","Köster, Sarah"],["dc.date.accessioned","2017-11-28T10:03:27Z"],["dc.date.available","2017-11-28T10:03:27Z"],["dc.date.issued","2016"],["dc.description.abstract","X-ray imaging of intact biological cells is emerging as a complementary method to visible light or electron microscopy. Owing to the high penetration depth and small wavelength of X-rays, it is possible to resolve subcellular structures at a resolution of a few nanometers. Here, we apply scanning X-ray nanodiffraction in combination with time-lapse bright-field microscopy to nuclei of 3T3 fibroblasts and thus relate the observed structures to specific phases in the cell division cycle. We scan the sample at a step size of 250 nm and analyze the individual diffraction patterns according to a generalized Porod’s law. Thus, we obtain information on the aggregation state of the nuclear DNA at a real space resolution on the order of the step size and in parallel structural information on the order of few nanometers. We are able to distinguish nucleoli, heterochromatin, and euchromatin in the nuclei and follow the compaction and decompaction during the cell division cycle."],["dc.identifier.doi","10.1021/acsnano.6b05034"],["dc.identifier.fs","623722"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/10593"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation.eissn","1936-086X"],["dc.relation.issn","1936-0851"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Köster (Cellular Biophysics)"],["dc.subject","biological cells; cell division cycle; DNA compaction; nanostructure; X-ray nanodiffraction"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","x-ray scattering"],["dc.subject.gro","cellular biophysics"],["dc.title","Following DNA Compaction During the Cell Cycle by X-ray Nanodiffraction"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","unknown"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]