Lakemeier, Stefan

[["dc.bibliographiccitation.firstpage","585"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Archives of Orthopaedic and Trauma Surgery"],["dc.bibliographiccitation.lastpage","592"],["dc.bibliographiccitation.volume","136"],["dc.contributor.author","Foelsch, Christian"],["dc.contributor.author","Federmann, Maike"],["dc.contributor.author","Lakemeier, Stefan"],["dc.contributor.author","Kuehn, Klaus D."],["dc.contributor.author","Kittinger, Clemens"],["dc.contributor.author","Kerwat, Martina"],["dc.contributor.author","Fuchs-Winkelmann, Susanne"],["dc.contributor.author","Paletta, Juergen R. J."],["dc.contributor.author","Roessler, Philip P."],["dc.date.accessioned","2018-11-07T10:16:24Z"],["dc.date.available","2018-11-07T10:16:24Z"],["dc.date.issued","2016"],["dc.description.abstract","Treatment of implant-associated osteomyelitis regularly involves the use of systemic antibiotics in addition to surgical intervention. However, it remains unclear if perioperative systemic application of bactericide substances can improve overall outcome in models of severe intramedullary infection. The present study investigated the use of systemic gentamicin in addition to a controlled local release from a highly lipophilic gentamicinpalmitate compound while the previous study showed efficacy of sole antibiotic implant-coating. Forty male Sprague-Dawley rats were divided into two groups receiving an intramedullary femoral injection of 10(2) CFU of a common methicillin susceptible Staphylococcus aureus strain (MSSA Rosenbach). Group I received an uncoated implant whereas group II received a coated implant. All animals received a single shot intraperitoneal application of gentamicinsulfate directly after wound closure while the historical control group III (n = 20) had no antibiotic treatment at all. Animals were observed for 28 and 42 days. Serum haptoglobin and relative weight gain were assessed as well as roll over cultures of explanted femur nails and histological scores of periprosthetic infection in dissected femora. Systemic application of gentamicin combined with antibiotic-coated implant did not further reduce bacterial growth significantly compared with systemic or local antibiotic application alone. Combined local and systemic therapy reduced serum haptoglobin significantly after day 7, 28 and 42 whereas systemic application alone did not compare to controls. Systemic perioperative and implant-associated application of antibiotics were both comparably effective to treat implant-associated infections whereas the combined antibiotic therapy further reduced systemic signs of infection time dependent."],["dc.description.sponsorship","Synthes GmbH, Umkirch, Germany"],["dc.identifier.doi","10.1007/s00402-016-2419-7"],["dc.identifier.isi","000373179000020"],["dc.identifier.pmid","26873243"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/41032"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","1434-3916"],["dc.relation.issn","0936-8051"],["dc.title","Systemic antibiotic therapy does not significantly improve outcome in a rat model of implant-associated osteomyelitis induced by Methicillin susceptible Staphylococcus aureus"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","400"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Journal of Spinal Disorders & Techniques"],["dc.bibliographiccitation.lastpage","406"],["dc.bibliographiccitation.volume","26"],["dc.contributor.author","Lakemeier, Stefan"],["dc.contributor.author","Schofer, Markus D."],["dc.contributor.author","Foltz, Lisa"],["dc.contributor.author","Schmid, Raphael"],["dc.contributor.author","Efe, Turgay"],["dc.contributor.author","Rohlfs, Jochen"],["dc.contributor.author","Fuchs-Winkelmann, Susanne"],["dc.contributor.author","El-Zayat, Bilal F."],["dc.contributor.author","Paletta, Juergen R. J."],["dc.contributor.author","Foelsch, Christian"],["dc.date.accessioned","2018-11-07T09:19:12Z"],["dc.date.available","2018-11-07T09:19:12Z"],["dc.date.issued","2013"],["dc.description.abstract","Study Design:Immunohistological study.Objective:To elucidate the role of matrix metalloproteinases (MMPs), hypoxia-inducible factor-1 (HIF), and vascular endothelial growth factor (VEGF) in the hypertrophied ligamentum flavum (LF) obtained from patients with lumbar spinal stenosis (LSS).Summary of Background Data:The most common spinal disorder in the elderly is LSS, which results in part from LF hypertrophy. Although prior histologic and immunochemical studies have been performed in this area, the pathophysiology of loss of elasticity and hypertrophy is not completely understood.Methods:LF samples of 38 patients with LSS were harvested during spinal decompression. Twelve LF samples obtained from patients with disk herniation and no visible degeneration on preoperative magnetic resonance imaging were obtained as controls. Samples were dehydrated and paraffin embedded. For immunohistochemical determination of VEGF, HIF, and MMPs 1, 3, and 9 expression, slices were stained with VEGF, HIF, and MMP antibody dilution. Neovessel density and number of elastic fibers were counted after Masson-Goldner staining. LF hypertrophy and cross-sectional area (CSA) were measured on T1-weighted magnetic resonance imaging.Results:MMPs 1, 3, 9 and VEGF expression were significantly increased in the hypertrophy group (P<0.05). HIF expression was negative in both groups. Vessel density was increased in the hypertrophy group, although this was not statistically significant. The number of elastic fibres was significantly higher in the control group. In the hypertrophy group, LF thickness was significantly increased, whereas CSA was significantly decreased. There was a statistical correlation between LF thickness, CSA, MMP, and VEGF expression in the hypertrophy group (P<0.05).Conclusions:LF hypertrophy is accompanied by increased MMPs 1, 3, 9 and VEGF expression. Neovessel density is increased in hypertrophied LF. HIF is not expressed in hypertrophied LF."],["dc.identifier.doi","10.1097/BSD.0b013e3182495b88"],["dc.identifier.isi","000326398500015"],["dc.identifier.pmid","22323068"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/28585"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Lippincott Williams & Wilkins"],["dc.relation.issn","1539-2465"],["dc.relation.issn","1536-0652"],["dc.title","Expression of Hypoxia-inducible Factor-1 alpha, Vascular Endothelial Growth Factor, and Matrix Metalloproteinases 1, 3, and 9 in Hypertrophied Ligamentum Flavum"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]