Lakemeier, Stefan

[["dc.bibliographiccitation.firstpage","1752"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","Biomolecules"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Westhoff, Christina C."],["dc.contributor.author","Peterlein, Christian-Dominik"],["dc.contributor.author","Daniel, Hanna"],["dc.contributor.author","Paletta, Juergen R."],["dc.contributor.author","Moll, Roland"],["dc.contributor.author","Ramaswamy, Annette"],["dc.contributor.author","Lakemeier, Stefan"],["dc.date.accessioned","2022-02-01T10:31:43Z"],["dc.date.available","2022-02-01T10:31:43Z"],["dc.date.issued","2021"],["dc.description.abstract","The most common spinal disorder in elderly is lumbar spinal stenosis (LSS), resulting partly from ligamentum flavum (LF) hypertrophy. Its pathophysiology is not completely understood. The present study wants to elucidate the role of estrogen receptor α (ER α) in fibroblasts of hypertrophied LF. LF samples of 38 patients with LSS were obtained during spinal decompression. Twelve LF samples from patients with disk herniation served as controls. Hematoxylin & Eosin (H&E) and Elastica stains and immunohistochemistry for ER α were performed. The proportions of fibrosis, loss and/or degeneration of elastic fibers and proliferation of collagen fibers were assessed according to the scores of Sairyo and Okuda. Group differences in the ER α and Sairyo and Okuda scores between patients and controls, male and female sex and absence and presence of additional orthopedic diagnoses were assessed with the Mann–Whitney U test. There was a tendency towards higher expression of ER α in LF fibroblasts in the hypertrophy group (p = 0.065). The Sairyo and Okuda scores were more severe for the hypertrophy group but, in general, not statistically relevant. There was no statistically relevant correlation between the expression of ER α and sex (p = 0.326). ER α expression was higher in patients with osteochondrosis but not statistically significant (p = 0.113). In patients with scoliosis, ER α expression was significantly lower (p = 0.044). LF hypertrophy may be accompanied by a higher expression of ER α in fibroblasts. No difference in ER α expression was observed regarding sex. Further studies are needed to clarify the biological and clinical significance of these findings."],["dc.description.abstract","The most common spinal disorder in elderly is lumbar spinal stenosis (LSS), resulting partly from ligamentum flavum (LF) hypertrophy. Its pathophysiology is not completely understood. The present study wants to elucidate the role of estrogen receptor α (ER α) in fibroblasts of hypertrophied LF. LF samples of 38 patients with LSS were obtained during spinal decompression. Twelve LF samples from patients with disk herniation served as controls. Hematoxylin & Eosin (H&E) and Elastica stains and immunohistochemistry for ER α were performed. The proportions of fibrosis, loss and/or degeneration of elastic fibers and proliferation of collagen fibers were assessed according to the scores of Sairyo and Okuda. Group differences in the ER α and Sairyo and Okuda scores between patients and controls, male and female sex and absence and presence of additional orthopedic diagnoses were assessed with the Mann–Whitney U test. There was a tendency towards higher expression of ER α in LF fibroblasts in the hypertrophy group (p = 0.065). The Sairyo and Okuda scores were more severe for the hypertrophy group but, in general, not statistically relevant. There was no statistically relevant correlation between the expression of ER α and sex (p = 0.326). ER α expression was higher in patients with osteochondrosis but not statistically significant (p = 0.113). In patients with scoliosis, ER α expression was significantly lower (p = 0.044). LF hypertrophy may be accompanied by a higher expression of ER α in fibroblasts. No difference in ER α expression was observed regarding sex. Further studies are needed to clarify the biological and clinical significance of these findings."],["dc.identifier.doi","10.3390/biom11121752"],["dc.identifier.pii","biom11121752"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/98931"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-517"],["dc.relation.eissn","2218-273X"],["dc.title","Expression of Estrogen Receptor Alpha and Evaluation of Histological Degeneration Scores in Fibroblasts of Hypertrophied Ligamentum Flavum: A Qualitative Study"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1663"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","International Orthopaedics"],["dc.bibliographiccitation.lastpage","1668"],["dc.bibliographiccitation.volume","40"],["dc.contributor.author","Braatz, Frank"],["dc.contributor.author","Staude, Daniel"],["dc.contributor.author","Klotz, Matthias C."],["dc.contributor.author","Wolf, Sebastian I."],["dc.contributor.author","Dreher, Thomas"],["dc.contributor.author","Lakemeier, Stefan"],["dc.date.accessioned","2018-11-07T10:11:07Z"],["dc.date.available","2018-11-07T10:11:07Z"],["dc.date.issued","2016"],["dc.description.abstract","Introduction Neurogenic hip dislocation is quite common in children with cerebral palsy (CP). The purpose of this study was to evaluate the long-term outcome of single-event multilevel surgery (SEMLS) in combination with hip reconstruction by using a periacetabular osteotomy as described by Dega concerning post-operative remodeling and plasticity of the femoral head post-operatively. Methods A total of 72 patients with CP as the primary disease and in whom a complex surgical hip reconstruction was performed during SEMLS between 1998 and 2004 were included in the study. There were 45 men and 27 women, with a median age of 7.6 (4.7-16.3) years at the time SEMLS was performed. The mean follow-up time was 7.7 years (4.9-11.8). X-rays were taken before and after surgery, and Rippstein 1 and 2 were used for follow-up. As the most reliable value for decentration, migration percentage (MP) as described by Reimers was used. To measure hip-joint cover at follow-up, the centre-edge angle was used. The hip was divided into four different categories according to sphericity and congruity. Using this approach, we could evaluate joint remodeling. Results Pre-operatively, the mean MP measured by X-ray was 68 %. Directly after surgery, this value decreased on average by 12 % and at the long-term follow-up was 16.0 % on average. A high rate of incongruence was observed on X-rays taken directly after surgery: 66 hip joints were classified as incongruent. The number of aspherical and incongruent joints decreased to 54 at the follow-up examination. Conclusion Data of our study with high plasticity of the hip joint suggest that even if the femoral head is deformed and a persistent incongruency after surgery is expected, hip reconstruction can be recommended."],["dc.identifier.doi","10.1007/s00264-015-3013-2"],["dc.identifier.isi","000380690500015"],["dc.identifier.pmid","26454662"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/39985"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","1432-5195"],["dc.relation.issn","0341-2695"],["dc.title","Hip-joint congruity after Dega osteotomy in patients with cerebral palsy: long-term results"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","111"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Archives of Orthopaedic and Trauma Surgery"],["dc.bibliographiccitation.lastpage","118"],["dc.bibliographiccitation.volume","135"],["dc.contributor.author","Baums, M. H."],["dc.contributor.author","Schminke, B."],["dc.contributor.author","Posmyk, A."],["dc.contributor.author","Miosge, N."],["dc.contributor.author","Klinger, H.-M."],["dc.contributor.author","Lakemeier, S."],["dc.date.accessioned","2018-08-20T12:33:19Z"],["dc.date.available","2018-08-20T12:33:19Z"],["dc.date.issued","2015"],["dc.description.abstract","The clinical superiority of the double-row technique is still a subject of controversial debate in rotator cuff repair. We hypothesised that the expression of different collagen types will differ between double-row and single-row rotator cuff repair indicating a faster healing response by the double-row technique."],["dc.identifier.doi","10.1007/s00402-014-2118-1"],["dc.identifier.pmid","25416099"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/15438"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation.eissn","1434-3916"],["dc.relation.eissn","0936-8051"],["dc.title","Effect of single- and double-row rotator cuff repair at the tendon-to-bone interface"],["dc.title.subtitle","Preliminary results using an in vivo sheep model"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","335"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","PM&R"],["dc.bibliographiccitation.lastpage","341"],["dc.bibliographiccitation.volume","4"],["dc.contributor.author","Foelsch, Christian"],["dc.contributor.author","Schloegel, Stefanie"],["dc.contributor.author","Lakemeier, Stefan"],["dc.contributor.author","Wolf, Udo"],["dc.contributor.author","Timmesfeld, Nina"],["dc.contributor.author","Skwara, Adrian"],["dc.date.accessioned","2018-11-07T09:10:33Z"],["dc.date.available","2018-11-07T09:10:33Z"],["dc.date.issued","2012"],["dc.description.abstract","Objective: To explore the reliability of the Zebris CMS 20 ultrasound analysis system with pointer application for measuring end-range flexion, end-range extension, and neutral kyphosis angle of the thoracic spine. Setting: The study was performed within the School of Physiotherapy in cooperation with the Orthopedic Department at a University Hospital. Participants: The thoracic spines of 28 healthy subjects were measured. Methods: Measurements for neutral kyphosis angle, end-range flexion, and end-range extension were taken once at each time point. The bone landmarks were palpated by one examiner and marked with a pointer containing 2 transmitters using a frequency of 40 kHz. A third transmitter was fixed to the pelvis, and 3 microphones were used as receiver. The real angle was calculated by the software. Bland-Altman plots with 95% limits of agreement, intraclass correlations (ICC), standard deviations of mean measurements, and standard error of measurements were used for statistical analyses. The test-retest reliability in this study was measured within a 24-hour interval. Main Outcome Measurements: Statistical parameters were used to judge reliability. Results: The mean kyphosis angle was 44.8 degrees with a standard deviation of 17.3 degrees at the first measurement and a mean of 45.8 degrees with a standard deviation of 16.2 degrees the following day. The ICC was high at 0.95 for the neutral kyphosis angle, and the Bland-Altman 95% limits of agreement were within clinical acceptable margins. The ICC was 0.71 for end-range flexion and 0.34 for end-range extension, whereas the Bland-Altman 95% limits of agreement were wider than with the static measurement of kyphosis. Compared with static measurements, the analysis of motion with 3-dimensional ultrasound showed an increased standard deviation for test-retest measurements. Conclusions: The test-retest reliability of ultrasound measuring of the neutral kyphosis angle of the thoracic spine was demonstrated within 24 hours. Bland-Altman 95% limits of agreement and the standard deviation of differences did not appear to be clinically acceptable for measuring flexion and extension. PM R 2012;4:335-341"],["dc.identifier.doi","10.1016/j.pmrj.2012.01.009"],["dc.identifier.isi","000305872800003"],["dc.identifier.pmid","22464951"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/26518"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Inc"],["dc.relation.issn","1934-1482"],["dc.title","Test-Retest Reliability of 3D Ultrasound Measurements of the Thoracic Spine"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","585"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Archives of Orthopaedic and Trauma Surgery"],["dc.bibliographiccitation.lastpage","592"],["dc.bibliographiccitation.volume","136"],["dc.contributor.author","Foelsch, Christian"],["dc.contributor.author","Federmann, Maike"],["dc.contributor.author","Lakemeier, Stefan"],["dc.contributor.author","Kuehn, Klaus D."],["dc.contributor.author","Kittinger, Clemens"],["dc.contributor.author","Kerwat, Martina"],["dc.contributor.author","Fuchs-Winkelmann, Susanne"],["dc.contributor.author","Paletta, Juergen R. J."],["dc.contributor.author","Roessler, Philip P."],["dc.date.accessioned","2018-11-07T10:16:24Z"],["dc.date.available","2018-11-07T10:16:24Z"],["dc.date.issued","2016"],["dc.description.abstract","Treatment of implant-associated osteomyelitis regularly involves the use of systemic antibiotics in addition to surgical intervention. However, it remains unclear if perioperative systemic application of bactericide substances can improve overall outcome in models of severe intramedullary infection. The present study investigated the use of systemic gentamicin in addition to a controlled local release from a highly lipophilic gentamicinpalmitate compound while the previous study showed efficacy of sole antibiotic implant-coating. Forty male Sprague-Dawley rats were divided into two groups receiving an intramedullary femoral injection of 10(2) CFU of a common methicillin susceptible Staphylococcus aureus strain (MSSA Rosenbach). Group I received an uncoated implant whereas group II received a coated implant. All animals received a single shot intraperitoneal application of gentamicinsulfate directly after wound closure while the historical control group III (n = 20) had no antibiotic treatment at all. Animals were observed for 28 and 42 days. Serum haptoglobin and relative weight gain were assessed as well as roll over cultures of explanted femur nails and histological scores of periprosthetic infection in dissected femora. Systemic application of gentamicin combined with antibiotic-coated implant did not further reduce bacterial growth significantly compared with systemic or local antibiotic application alone. Combined local and systemic therapy reduced serum haptoglobin significantly after day 7, 28 and 42 whereas systemic application alone did not compare to controls. Systemic perioperative and implant-associated application of antibiotics were both comparably effective to treat implant-associated infections whereas the combined antibiotic therapy further reduced systemic signs of infection time dependent."],["dc.description.sponsorship","Synthes GmbH, Umkirch, Germany"],["dc.identifier.doi","10.1007/s00402-016-2419-7"],["dc.identifier.isi","000373179000020"],["dc.identifier.pmid","26873243"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/41032"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","1434-3916"],["dc.relation.issn","0936-8051"],["dc.title","Systemic antibiotic therapy does not significantly improve outcome in a rat model of implant-associated osteomyelitis induced by Methicillin susceptible Staphylococcus aureus"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","228"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Anesthesia & Analgesia"],["dc.bibliographiccitation.lastpage","235"],["dc.bibliographiccitation.volume","117"],["dc.contributor.author","Lakemeier, Stefan"],["dc.contributor.author","Lind, Marcel"],["dc.contributor.author","Schultz, Wolfgang"],["dc.contributor.author","Fuchs-Winkelmann, Susanne"],["dc.contributor.author","Timmesfeld, Nina"],["dc.contributor.author","Foelsch, Christian"],["dc.contributor.author","Peterlein, Christian D."],["dc.date.accessioned","2018-11-07T09:22:37Z"],["dc.date.available","2018-11-07T09:22:37Z"],["dc.date.issued","2013"],["dc.description.abstract","BACKGROUND: Lumbar facet joint degeneration is a source of chronic low back pain, with an incidence of 15% to 45% among patients with low back pain. Various therapeutic techniques in the treatment of facet-related pain have been described in the literature, including intraarticular lumbar facet joint steroid injections and radiofrequency denervation. In this study, we compared the effectiveness of intraarticular facet joint steroid injections and radiofrequency denervation. METHODS: Our randomized, double-blind, controlled study included patients who received intraarticular steroid infiltrations in the lumbar facet joints (L3/L4-L5/S1) and patients who underwent radiofrequency denervation of L3/L4-L5/S1 segments. The inclusion criteria were based first on magnetic resonance imaging findings showing hypertrophy of the facet joints L3/L4-L5/S1 and a positive response to an intraarticular test infiltration of the facet joints L3/L4-L5/S1 with local anesthetics. The primary end point was the Roland-Morris Questionnaire. Secondary end points were the visual analog scale and the Oswestry Disability Index. All outcome assessments were performed at baseline and at 6 months. RESULTS: Fifty-six patients were randomized; 24 of 29 patients in the steroid injection group and 26 of 27 patients in the denervation group completed the 6-month follow-up. Pain relief and functional improvement were observed in both groups. There were no significant differences between the 2 groups for the primary end point (95% confidence interval [CI], -3 to 4) and for both secondary end points (95% CI for visual analog scale, -2 to 1; 95% CI for Oswestry Disability Index, -18 to 0). CONCLUSIONS: Intraarticular steroid infiltration or radiofrequency denervation appear to be a managing option for chronic function-limiting low back pain of facet origin with favorable short- and midterm results in terms of pain relief and function improvement, but improvements were similar in both groups."],["dc.identifier.doi","10.1213/ANE.0b013e3182910c4d"],["dc.identifier.isi","000326512300034"],["dc.identifier.pmid","23632051"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/29390"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Lippincott Williams & Wilkins"],["dc.relation.issn","1526-7598"],["dc.relation.issn","0003-2999"],["dc.title","A Comparison of Intraarticular Lumbar Facet Joint Steroid Injections and Lumbar Facet Joint Radiofrequency Denervation in the Treatment of Low Back Pain: A Randomized, Controlled, Double-Blind Trial"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","400"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Journal of Spinal Disorders & Techniques"],["dc.bibliographiccitation.lastpage","406"],["dc.bibliographiccitation.volume","26"],["dc.contributor.author","Lakemeier, Stefan"],["dc.contributor.author","Schofer, Markus D."],["dc.contributor.author","Foltz, Lisa"],["dc.contributor.author","Schmid, Raphael"],["dc.contributor.author","Efe, Turgay"],["dc.contributor.author","Rohlfs, Jochen"],["dc.contributor.author","Fuchs-Winkelmann, Susanne"],["dc.contributor.author","El-Zayat, Bilal F."],["dc.contributor.author","Paletta, Juergen R. J."],["dc.contributor.author","Foelsch, Christian"],["dc.date.accessioned","2018-11-07T09:19:12Z"],["dc.date.available","2018-11-07T09:19:12Z"],["dc.date.issued","2013"],["dc.description.abstract","Study Design:Immunohistological study.Objective:To elucidate the role of matrix metalloproteinases (MMPs), hypoxia-inducible factor-1 (HIF), and vascular endothelial growth factor (VEGF) in the hypertrophied ligamentum flavum (LF) obtained from patients with lumbar spinal stenosis (LSS).Summary of Background Data:The most common spinal disorder in the elderly is LSS, which results in part from LF hypertrophy. Although prior histologic and immunochemical studies have been performed in this area, the pathophysiology of loss of elasticity and hypertrophy is not completely understood.Methods:LF samples of 38 patients with LSS were harvested during spinal decompression. Twelve LF samples obtained from patients with disk herniation and no visible degeneration on preoperative magnetic resonance imaging were obtained as controls. Samples were dehydrated and paraffin embedded. For immunohistochemical determination of VEGF, HIF, and MMPs 1, 3, and 9 expression, slices were stained with VEGF, HIF, and MMP antibody dilution. Neovessel density and number of elastic fibers were counted after Masson-Goldner staining. LF hypertrophy and cross-sectional area (CSA) were measured on T1-weighted magnetic resonance imaging.Results:MMPs 1, 3, 9 and VEGF expression were significantly increased in the hypertrophy group (P<0.05). HIF expression was negative in both groups. Vessel density was increased in the hypertrophy group, although this was not statistically significant. The number of elastic fibres was significantly higher in the control group. In the hypertrophy group, LF thickness was significantly increased, whereas CSA was significantly decreased. There was a statistical correlation between LF thickness, CSA, MMP, and VEGF expression in the hypertrophy group (P<0.05).Conclusions:LF hypertrophy is accompanied by increased MMPs 1, 3, 9 and VEGF expression. Neovessel density is increased in hypertrophied LF. HIF is not expressed in hypertrophied LF."],["dc.identifier.doi","10.1097/BSD.0b013e3182495b88"],["dc.identifier.isi","000326398500015"],["dc.identifier.pmid","22323068"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/28585"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Lippincott Williams & Wilkins"],["dc.relation.issn","1539-2465"],["dc.relation.issn","1536-0652"],["dc.title","Expression of Hypoxia-inducible Factor-1 alpha, Vascular Endothelial Growth Factor, and Matrix Metalloproteinases 1, 3, and 9 in Hypertrophied Ligamentum Flavum"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]