Witte, Mirko

[["dc.bibliographiccitation.artnumber","1000107"],["dc.bibliographiccitation.journal","Frontiers in Neuroanatomy"],["dc.bibliographiccitation.volume","16"],["dc.contributor.affiliation","Staiger, Jochen F.; Institute for Neuroanatomy, Universitätsmedizin Göttingen, Georg-August-Universität Göttingen, Göttingen, Germany"],["dc.contributor.affiliation","Sachkova, Alexandra; Institute for Neuroanatomy, Universitätsmedizin Göttingen, Georg-August-Universität Göttingen, Göttingen, Germany"],["dc.contributor.affiliation","Möck, Martin; Institute for Neuroanatomy, Universitätsmedizin Göttingen, Georg-August-Universität Göttingen, Göttingen, Germany"],["dc.contributor.affiliation","Guy, Julien; Institute for Neuroanatomy, Universitätsmedizin Göttingen, Georg-August-Universität Göttingen, Göttingen, Germany"],["dc.contributor.affiliation","Witte, Mirko; Institute for Neuroanatomy, Universitätsmedizin Göttingen, Georg-August-Universität Göttingen, Göttingen, Germany"],["dc.contributor.author","Staiger, Jochen F."],["dc.contributor.author","Sachkova, Alexandra"],["dc.contributor.author","Möck, Martin"],["dc.contributor.author","Guy, Julien"],["dc.contributor.author","Witte, Mirko"],["dc.date.accessioned","2022-12-01T08:31:33Z"],["dc.date.available","2022-12-01T08:31:33Z"],["dc.date.issued","2022"],["dc.date.updated","2022-11-11T13:11:49Z"],["dc.description.abstract","Reelin is a large extracellular glycoprotein that is secreted by Cajal-Retzius cells during embryonic development to regulate neuronal migration and cell proliferation but it also seems to regulate ion channel distribution and synaptic vesicle release properties of excitatory neurons well into adulthood. Mouse mutants with a compromised reelin signaling cascade show a highly disorganized neocortex but the basic connectional features of the displaced excitatory principal cells seem to be relatively intact. Very little is known, however, about the intrinsic electrophysiological and morphological properties of individual cells in the reeler cortex. Repetitive burst-spiking (RB) is a unique property of large, thick-tufted pyramidal cells of wild-type layer Vb exclusively, which project to several subcortical targets. In addition, they are known to possess sparse but far-reaching intracortical recurrent collaterals. Here, we compared the electrophysiological properties and morphological features of neurons in the reeler primary somatosensory cortex with those of wild-type controls. Whereas in wild-type mice, RB pyramidal cells were only detected in layer Vb, and the vast majority of reeler RB pyramidal cells were found in the superficial third of the cortical depth. There were no obvious differences in the intrinsic electrophysiological properties and basic morphological features (such as soma size or the number of dendrites) were also well preserved. However, the spatial orientation of the entire dendritic tree was highly variable in the reeler neocortex, whereas it was completely stereotyped in wild-type mice. It seems that basic quantitative features of layer Vb-fated RB pyramidal cells are well conserved in the highly disorganized mutant neocortex, whereas qualitative morphological features vary, possibly to properly orient toward the appropriate input pathways, which are known to show an atypical oblique path through the reeler cortex. The oblique dendritic orientation thus presumably reflects a re-orientation of dendritic input domains toward spatially highly disorganized afferent projections."],["dc.identifier.doi","10.3389/fnana.2022.1000107"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/118199"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-621"],["dc.publisher","Frontiers Media S.A."],["dc.relation.eissn","1662-5129"],["dc.relation.isreplacedby","hdl:2/118199"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0/"],["dc.title","Repetitively burst-spiking neurons in reeler mice show conserved but also highly variable morphological features of layer Vb-fated “thick-tufted” pyramidal cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","4854"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","Cerebral Cortex"],["dc.bibliographiccitation.lastpage","4868"],["dc.bibliographiccitation.volume","25"],["dc.contributor.author","Proenneke, Alvar"],["dc.contributor.author","Scheuer, Bianca"],["dc.contributor.author","Wagener, Robin Jan"],["dc.contributor.author","Moeck, Martin"],["dc.contributor.author","Witte, Mirko"],["dc.contributor.author","Staiger, Jochen F."],["dc.date.accessioned","2018-11-07T09:48:24Z"],["dc.date.available","2018-11-07T09:48:24Z"],["dc.date.issued","2015"],["dc.description.abstract","Neocortical GABAergic interneurons have a profound impact on cortical circuitry and its information processing capacity. Distinct subgroups of inhibitory interneurons can be distinguished by molecular markers, such as parvalbumin, somatostatin, and vasoactive intestinal polypeptide (VIP). Among these, VIP-expressing interneurons sparked a substantial interest since these neurons seem to operate disinhibitory circuit motifs found in all major neocortical areas. Several of these recent studies used transgenic Vip-ires-cre mice to specifically target the population of VIP-expressing interneurons. This makes it necessary to elucidate in detail the sensitivity and specificity of Cre expression for VIP neurons in these animals. Thus, we quantitatively compared endogenous tdTomato with Vip fluorescence in situ hybridization and alpha VIP immunohistochemistry in the barrel cortex of VIPcre/tdTomato mice in a layer-specific manner. We show that VIPcre/tdTomato mice are highly sensitive and specific for the entire population of VIP-expressing neurons. In the barrel cortex, approximately 13% of all GABAergic neurons are VIP expressing. Most VIP neurons are found in layer II/III (similar to 60%), whereas approximately 40% are found in the other layers of the barrel cortex. Layer II/III VIP neurons are significantly different from VIP neurons in layers IV-VI in several morphological and membrane properties, which suggest layer-dependent differences in functionality."],["dc.identifier.doi","10.1093/cercor/bhv202"],["dc.identifier.isi","000366463800019"],["dc.identifier.pmid","26420784"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12750"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/35296"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press Inc"],["dc.relation.issn","1460-2199"],["dc.relation.issn","1047-3211"],["dc.rights","CC BY-NC 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc/4.0"],["dc.title","Characterizing VIP Neurons in the Barrel Cortex of VIPcre/tdTomato Mice Reveals Layer-Specific Differences"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","820"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Cerebral Cortex"],["dc.bibliographiccitation.lastpage","837"],["dc.bibliographiccitation.volume","26"],["dc.contributor.author","Wagener, Robin Jan"],["dc.contributor.author","Witte, Mirko"],["dc.contributor.author","Guy, Julien"],["dc.contributor.author","Mingo-Moreno, Nieves"],["dc.contributor.author","Kuegler, Sebastian"],["dc.contributor.author","Staiger, Jochen F."],["dc.date.accessioned","2018-11-07T10:18:31Z"],["dc.date.available","2018-11-07T10:18:31Z"],["dc.date.issued","2016"],["dc.description.abstract","Neuronal wiring is key to proper neural information processing. Tactile information from the rodent's whiskers reaches the cortex via distinct anatomical pathways. The lemniscal pathway relays whisking and touch information from the ventral posteromedial thalamic nucleus to layer IV of the primary somatosensory \"barrel\" cortex. The disorganized neocortex of the reeler mouse is a model system that should severely compromise the ingrowth of thalamocortical axons (TCAs) into the cortex. Moreover, it could disrupt intracortical wiring. We found that neuronal intermingling within the reeler barrel cortex substantially exceeded previous descriptions, leading to the loss of layers. However, viral tracing revealed that TCAs still specifically targeted transgenically labeled spiny layer IV neurons. Slice electrophysiology and optogenetics proved that these connections represent functional synapses. In addition, we assessed intracortical activation via immediate-early-gene expression resulting from a behavioral exploration task. The cellular composition of activated neuronal ensembles suggests extensive similarities in intracolumnar information processing in the wild-type and reeler brains. We conclude that extensive ectopic positioning of neuronal partners can be compensated for by cell-autonomous mechanisms that allow for the establishment of proper connectivity. Thus, genetic neuronal fate seems to be of greater importance for correct cortical wiring than radial neuronal position."],["dc.identifier.doi","10.1093/cercor/bhv257"],["dc.identifier.isi","000371522500030"],["dc.identifier.pmid","26564256"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14147"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/41460"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press Inc"],["dc.relation.issn","1460-2199"],["dc.relation.issn","1047-3211"],["dc.rights","CC BY-NC 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc/4.0"],["dc.title","Thalamocortical Connections Drive Intracortical Activation of Functional Columns in the Mislaminated Reeler Somatosensory Cortex"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3450"],["dc.bibliographiccitation.issue","13"],["dc.bibliographiccitation.journal","Cell Reports"],["dc.bibliographiccitation.lastpage","3461.e8"],["dc.bibliographiccitation.volume","28"],["dc.contributor.author","Hafner, Georg"],["dc.contributor.author","Witte, Mirko"],["dc.contributor.author","Guy, Julien"],["dc.contributor.author","Subhashini, Nidhi"],["dc.contributor.author","Fenno, Lief E."],["dc.contributor.author","Ramakrishnan, Charu"],["dc.contributor.author","Kim, Yoon Seok"],["dc.contributor.author","Deisseroth, Karl"],["dc.contributor.author","Callaway, Edward M."],["dc.contributor.author","Oberhuber, Martina"],["dc.contributor.author","Conzelmann, Karl-Klaus"],["dc.contributor.author","Staiger, Jochen F."],["dc.date.accessioned","2020-12-10T14:23:02Z"],["dc.date.available","2020-12-10T14:23:02Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1016/j.celrep.2019.08.064"],["dc.identifier.issn","2211-1247"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16830"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/71810"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.intern","Merged from goescholar"],["dc.rights","CC BY-NC-ND 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc-nd/4.0"],["dc.title","Mapping Brain-Wide Afferent Inputs of Parvalbumin-Expressing GABAergic Neurons in Barrel Cortex Reveals Local and Long-Range Circuit Motifs"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","244"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Journal of Comparative Neurology"],["dc.bibliographiccitation.lastpage","260"],["dc.bibliographiccitation.volume","528"],["dc.contributor.author","Zhou, Xiaojuan"],["dc.contributor.author","Mansori, Ima"],["dc.contributor.author","Fischer, Tatjana"],["dc.contributor.author","Witte, Mirko"],["dc.contributor.author","Staiger, Jochen F."],["dc.date.accessioned","2019-11-27T13:23:59Z"],["dc.date.accessioned","2021-10-27T13:21:38Z"],["dc.date.available","2019-11-27T13:23:59Z"],["dc.date.available","2021-10-27T13:21:38Z"],["dc.date.issued","2019"],["dc.description.abstract","Somatostatin-expressing (SST+) cells form the second largest subpopulation of neocortical GABAergic neurons that contain diverse subtypes, which participate in layer-specific cortical circuits. Martinotti cells, as the most abundant subtype of SST+ interneurons, are mainly located in layers II/III and V/VI, and are characterized by dense axonal arborizations in layer I. GFP-expressing inhibitory neurons (GIN), representing a fraction of mainly upper layer SST+ interneurons in various cortical areas, were recently claimed to include both Martinotti cells and non-Martinotti cells. This makes it necessary to examine in detail the morphology and synaptic innervation pattern of the GIN cells, in order to better predict their functional implications. In our study, we characterized the neurochemical specificity, somatodendritic morphology, synaptic ultrastructure as well as synaptic innervation pattern of GIN cells in the barrel cortex in a layer-specific manner. We showed that GIN cells account for 44% of the SST+ interneurons in layer II/III and around 35% in layers IV and Va. There are 29% of GIN cells coexpressing calretinin with 54% in layer II/III, 8% in layer IV, and 13% in layer V. They have diverse somatodendritic configurations and form relatively small synapses across all examined layers. They almost exclusively innervate dendrites of excitatory cells, preferentially targeting distal apical dendrites and apical dendritic tufts of pyramidal neurons in layer I, and rarely target other inhibitory neurons. In summary, our study reveals unique features in terms of the morphology and output of GIN cells, which can help to better understand their diversity and structure-function relationships."],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft http://dx.doi.org/10.13039/501100001659"],["dc.identifier.doi","10.1002/cne.24756"],["dc.identifier.eissn","1096-9861"],["dc.identifier.issn","0021-9967"],["dc.identifier.pmid","31407339"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16755"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/92036"],["dc.language.iso","en"],["dc.notes.intern","Migrated from goescholar"],["dc.publisher","John Wiley \\u0026 Sons, Inc."],["dc.relation.eissn","1096-9861"],["dc.relation.issn","1096-9861"],["dc.relation.issn","0021-9967"],["dc.relation.orgunit","Universitätsmedizin Göttingen"],["dc.rights","CC BY-NC 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc/4.0"],["dc.subject.ddc","610"],["dc.title","Characterizing the morphology of somatostatin‐expressing interneurons and their synaptic innervation pattern in the barrel cortex of the GFP‐expressing inhibitory neurons mouse"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]