Witte, Mirko

[["dc.bibliographiccitation.firstpage","347"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Nature Medicine"],["dc.bibliographiccitation.lastpage","354"],["dc.bibliographiccitation.volume","23"],["dc.contributor.author","Koukouli, Fani"],["dc.contributor.author","Rooy, Marie"],["dc.contributor.author","Tziotis, Dimitrios"],["dc.contributor.author","Sailor, Kurt A."],["dc.contributor.author","O'Neill, Heidi C."],["dc.contributor.author","Levenga, Josien"],["dc.contributor.author","Witte, Mirko"],["dc.contributor.author","Nilges, Michael"],["dc.contributor.author","Changeux, Jean-Pierre"],["dc.contributor.author","Hoeffer, Charles A."],["dc.contributor.author","Stitzel, Jerry A."],["dc.contributor.author","Gutkin, Boris S."],["dc.contributor.author","DiGregorio, David A."],["dc.contributor.author","Maskos, Uwe"],["dc.date.accessioned","2018-11-07T10:27:06Z"],["dc.date.available","2018-11-07T10:27:06Z"],["dc.date.issued","2017"],["dc.description.abstract","The prefrontal cortex (PFC) underlies higher cognitive processes(1) that are modulated by nicotinic acetylcholine receptor (nAChR) activation by cholinergic inputs(2). PFC spontaneous default activity(3) is altered in neuropsychiatric disorders(4), including schizophrenia(5) a disorder that can be accompanied by heavy smoking(6). Recently, genome-wide association studies (GWAS) identified single-nucleotide polymorphisms (SNPs) in the human CHRNA5 gene, encoding the alpha 5 nAChR subunit, that increase the risks for both smoking and schizophrenia(7'8). Mice with altered nAChR gene function exhibit PFC-dependent behavioral deficits(9-11), but it is unknown how the corresponding human polymorphisms alter the cellular and circuit mechanisms underlying behavior. Here we show that mice expressing a human 5 SNP exhibit neurocognitive behavioral deficits in social interaction and sensorimotor gating tasks. Two-photon calcium imaging in awake mouse models showed that nicotine can differentially influence PFC pyramidal cell activity by nAChR modulation of layer II/III hierarchical inhibitory circuits. In 5-SNP-expressing and 5-knockout mice, lower activity of vasoactive intestinal polypeptide (VIP) interneurons resulted in an increased somatostatin (SOM) interneuron inhibitory drive over layer II/III pyramidal neurons. The decreased activity observed in 5-SNP-expressing mice resembles the hypofrontality observed in patients with psychiatric disorders, including schizophrenia and addiction(5,12).Chronic nicotine administration reversed this hypofrontality, suggesting that administration of nicotine may represent a therapeutic strategy for the treatment of schizophrenia, and a physiological basis for the tendency of patients with schizophrenia to self-medicate by smoking(13)."],["dc.identifier.doi","10.1038/nm.4274"],["dc.identifier.isi","000395848400015"],["dc.identifier.pmid","28112735"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/43182"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Nature Publishing Group"],["dc.relation.issn","1546-170X"],["dc.relation.issn","1078-8956"],["dc.title","Nicotine reverses hypofrontality in animal models of addiction and schizophrenia"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","1000107"],["dc.bibliographiccitation.journal","Frontiers in Neuroanatomy"],["dc.bibliographiccitation.volume","16"],["dc.contributor.affiliation","Staiger, Jochen F.; Institute for Neuroanatomy, Universitätsmedizin Göttingen, Georg-August-Universität Göttingen, Göttingen, Germany"],["dc.contributor.affiliation","Sachkova, Alexandra; Institute for Neuroanatomy, Universitätsmedizin Göttingen, Georg-August-Universität Göttingen, Göttingen, Germany"],["dc.contributor.affiliation","Möck, Martin; Institute for Neuroanatomy, Universitätsmedizin Göttingen, Georg-August-Universität Göttingen, Göttingen, Germany"],["dc.contributor.affiliation","Guy, Julien; Institute for Neuroanatomy, Universitätsmedizin Göttingen, Georg-August-Universität Göttingen, Göttingen, Germany"],["dc.contributor.affiliation","Witte, Mirko; Institute for Neuroanatomy, Universitätsmedizin Göttingen, Georg-August-Universität Göttingen, Göttingen, Germany"],["dc.contributor.author","Staiger, Jochen F."],["dc.contributor.author","Sachkova, Alexandra"],["dc.contributor.author","Möck, Martin"],["dc.contributor.author","Guy, Julien"],["dc.contributor.author","Witte, Mirko"],["dc.date.accessioned","2022-12-01T08:31:33Z"],["dc.date.available","2022-12-01T08:31:33Z"],["dc.date.issued","2022"],["dc.date.updated","2022-11-11T13:11:49Z"],["dc.description.abstract","Reelin is a large extracellular glycoprotein that is secreted by Cajal-Retzius cells during embryonic development to regulate neuronal migration and cell proliferation but it also seems to regulate ion channel distribution and synaptic vesicle release properties of excitatory neurons well into adulthood. Mouse mutants with a compromised reelin signaling cascade show a highly disorganized neocortex but the basic connectional features of the displaced excitatory principal cells seem to be relatively intact. Very little is known, however, about the intrinsic electrophysiological and morphological properties of individual cells in the reeler cortex. Repetitive burst-spiking (RB) is a unique property of large, thick-tufted pyramidal cells of wild-type layer Vb exclusively, which project to several subcortical targets. In addition, they are known to possess sparse but far-reaching intracortical recurrent collaterals. Here, we compared the electrophysiological properties and morphological features of neurons in the reeler primary somatosensory cortex with those of wild-type controls. Whereas in wild-type mice, RB pyramidal cells were only detected in layer Vb, and the vast majority of reeler RB pyramidal cells were found in the superficial third of the cortical depth. There were no obvious differences in the intrinsic electrophysiological properties and basic morphological features (such as soma size or the number of dendrites) were also well preserved. However, the spatial orientation of the entire dendritic tree was highly variable in the reeler neocortex, whereas it was completely stereotyped in wild-type mice. It seems that basic quantitative features of layer Vb-fated RB pyramidal cells are well conserved in the highly disorganized mutant neocortex, whereas qualitative morphological features vary, possibly to properly orient toward the appropriate input pathways, which are known to show an atypical oblique path through the reeler cortex. The oblique dendritic orientation thus presumably reflects a re-orientation of dendritic input domains toward spatially highly disorganized afferent projections."],["dc.identifier.doi","10.3389/fnana.2022.1000107"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/118199"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-621"],["dc.publisher","Frontiers Media S.A."],["dc.relation.eissn","1662-5129"],["dc.relation.isreplacedby","hdl:2/118199"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0/"],["dc.title","Repetitively burst-spiking neurons in reeler mice show conserved but also highly variable morphological features of layer Vb-fated “thick-tufted” pyramidal cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","no"],["dc.bibliographiccitation.issue","23"],["dc.bibliographiccitation.journal","ChemInform"],["dc.bibliographiccitation.lastpage","no"],["dc.bibliographiccitation.volume","31"],["dc.contributor.author","Witt, M."],["dc.contributor.author","Roesky, H. W."],["dc.date.accessioned","2021-12-08T12:28:38Z"],["dc.date.available","2021-12-08T12:28:38Z"],["dc.date.issued","2010"],["dc.identifier.doi","10.1002/chin.200023284"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/95774"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-476"],["dc.relation.eissn","1522-2667"],["dc.relation.issn","0931-7597"],["dc.rights.uri","http://doi.wiley.com/10.1002/tdm_license_1.1"],["dc.title","ChemInform Abstract: Organoaluminum Chemistry at the Forefront of Research and Development"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","4854"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","Cerebral Cortex"],["dc.bibliographiccitation.lastpage","4868"],["dc.bibliographiccitation.volume","25"],["dc.contributor.author","Proenneke, Alvar"],["dc.contributor.author","Scheuer, Bianca"],["dc.contributor.author","Wagener, Robin Jan"],["dc.contributor.author","Moeck, Martin"],["dc.contributor.author","Witte, Mirko"],["dc.contributor.author","Staiger, Jochen F."],["dc.date.accessioned","2018-11-07T09:48:24Z"],["dc.date.available","2018-11-07T09:48:24Z"],["dc.date.issued","2015"],["dc.description.abstract","Neocortical GABAergic interneurons have a profound impact on cortical circuitry and its information processing capacity. Distinct subgroups of inhibitory interneurons can be distinguished by molecular markers, such as parvalbumin, somatostatin, and vasoactive intestinal polypeptide (VIP). Among these, VIP-expressing interneurons sparked a substantial interest since these neurons seem to operate disinhibitory circuit motifs found in all major neocortical areas. Several of these recent studies used transgenic Vip-ires-cre mice to specifically target the population of VIP-expressing interneurons. This makes it necessary to elucidate in detail the sensitivity and specificity of Cre expression for VIP neurons in these animals. Thus, we quantitatively compared endogenous tdTomato with Vip fluorescence in situ hybridization and alpha VIP immunohistochemistry in the barrel cortex of VIPcre/tdTomato mice in a layer-specific manner. We show that VIPcre/tdTomato mice are highly sensitive and specific for the entire population of VIP-expressing neurons. In the barrel cortex, approximately 13% of all GABAergic neurons are VIP expressing. Most VIP neurons are found in layer II/III (similar to 60%), whereas approximately 40% are found in the other layers of the barrel cortex. Layer II/III VIP neurons are significantly different from VIP neurons in layers IV-VI in several morphological and membrane properties, which suggest layer-dependent differences in functionality."],["dc.identifier.doi","10.1093/cercor/bhv202"],["dc.identifier.isi","000366463800019"],["dc.identifier.pmid","26420784"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12750"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/35296"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press Inc"],["dc.relation.issn","1460-2199"],["dc.relation.issn","1047-3211"],["dc.rights","CC BY-NC 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc/4.0"],["dc.title","Characterizing VIP Neurons in the Barrel Cortex of VIPcre/tdTomato Mice Reveals Layer-Specific Differences"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Frontiers in Neuroanatomy"],["dc.bibliographiccitation.volume","13"],["dc.contributor.author","Almási, Zsuzsanna"],["dc.contributor.author","Dávid, Csaba"],["dc.contributor.author","Witte, Mirko"],["dc.contributor.author","Staiger, Jochen F."],["dc.date.accessioned","2020-12-10T18:44:30Z"],["dc.date.available","2020-12-10T18:44:30Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.3389/fnana.2019.00045"],["dc.identifier.eissn","1662-5129"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/78478"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Distribution Patterns of Three Molecularly Defined Classes of GABAergic Neurons Across Columnar Compartments in Mouse Barrel Cortex"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Cerebral Cortex"],["dc.contributor.author","Prönneke, Alvar"],["dc.contributor.author","Witte, Mirko"],["dc.contributor.author","Möck, Martin"],["dc.contributor.author","Staiger, Jochen F"],["dc.date.accessioned","2020-12-10T18:18:41Z"],["dc.date.available","2020-12-10T18:18:41Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1093/cercor/bhz102"],["dc.identifier.eissn","1460-2199"],["dc.identifier.issn","1047-3211"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/75092"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Neuromodulation Leads to a Burst-Tonic Switch in a Subset of VIP Neurons in Mouse Primary Somatosensory (Barrel) Cortex"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1427"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Cerebral Cortex"],["dc.bibliographiccitation.lastpage","1443"],["dc.bibliographiccitation.volume","31"],["dc.contributor.author","Hafner, Georg"],["dc.contributor.author","Guy, Julien"],["dc.contributor.author","Witte, Mirko"],["dc.contributor.author","Truschow, Pavel"],["dc.contributor.author","Rüppel, Alina"],["dc.contributor.author","Sirmpilatze, Nikoloz"],["dc.contributor.author","Dadarwal, Rakshit"],["dc.contributor.author","Boretius, Susann"],["dc.contributor.author","Staiger, Jochen F"],["dc.date.accessioned","2021-06-01T09:41:52Z"],["dc.date.available","2021-06-01T09:41:52Z"],["dc.date.issued","2020"],["dc.description.abstract","Abstract The neocortex is composed of layers. Whether layers constitute an essential framework for the formation of functional circuits is not well understood. We investigated the brain-wide input connectivity of vasoactive intestinal polypeptide (VIP) expressing neurons in the reeler mouse. This mutant is characterized by a migration deficit of cortical neurons so that no layers are formed. Still, neurons retain their properties and reeler mice show little cognitive impairment. We focused on VIP neurons because they are known to receive strong long-range inputs and have a typical laminar bias toward upper layers. In reeler, these neurons are more dispersed across the cortex. We mapped the brain-wide inputs of VIP neurons in barrel cortex of wild-type and reeler mice with rabies virus tracing. Innervation by subcortical inputs was not altered in reeler, in contrast to the cortical circuitry. Numbers of long-range ipsilateral cortical inputs were reduced in reeler, while contralateral inputs were strongly increased. Reeler mice had more callosal projection neurons. Hence, the corpus callosum was larger in reeler as shown by structural imaging. We argue that, in the absence of cortical layers, circuits with subcortical structures are maintained but cortical neurons establish a different network that largely preserves cognitive functions."],["dc.identifier.doi","10.1093/cercor/bhaa280"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/85068"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.eissn","1460-2199"],["dc.relation.issn","1047-3211"],["dc.title","Increased Callosal Connectivity in Reeler Mice Revealed by Brain-Wide Input Mapping of VIP Neurons in Barrel Cortex"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Cerebral Cortex"],["dc.contributor.author","Guy, Julien"],["dc.contributor.author","Sachkova, Alexandra"],["dc.contributor.author","Möck, Martin"],["dc.contributor.author","Witte, Mirko"],["dc.contributor.author","Wagener, Robin J."],["dc.contributor.author","Staiger, Jochen F."],["dc.date.accessioned","2020-12-10T18:18:32Z"],["dc.date.available","2020-12-10T18:18:32Z"],["dc.date.issued","2016"],["dc.identifier.doi","10.1093/cercor/bhw281"],["dc.identifier.eissn","1460-2199"],["dc.identifier.issn","1047-3211"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/75088"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Intracortical Network Effects Preserve Thalamocortical Input Efficacy in a Cortex Without Layers"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","820"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Cerebral Cortex"],["dc.bibliographiccitation.lastpage","837"],["dc.bibliographiccitation.volume","26"],["dc.contributor.author","Wagener, Robin Jan"],["dc.contributor.author","Witte, Mirko"],["dc.contributor.author","Guy, Julien"],["dc.contributor.author","Mingo-Moreno, Nieves"],["dc.contributor.author","Kuegler, Sebastian"],["dc.contributor.author","Staiger, Jochen F."],["dc.date.accessioned","2018-11-07T10:18:31Z"],["dc.date.available","2018-11-07T10:18:31Z"],["dc.date.issued","2016"],["dc.description.abstract","Neuronal wiring is key to proper neural information processing. Tactile information from the rodent's whiskers reaches the cortex via distinct anatomical pathways. The lemniscal pathway relays whisking and touch information from the ventral posteromedial thalamic nucleus to layer IV of the primary somatosensory \"barrel\" cortex. The disorganized neocortex of the reeler mouse is a model system that should severely compromise the ingrowth of thalamocortical axons (TCAs) into the cortex. Moreover, it could disrupt intracortical wiring. We found that neuronal intermingling within the reeler barrel cortex substantially exceeded previous descriptions, leading to the loss of layers. However, viral tracing revealed that TCAs still specifically targeted transgenically labeled spiny layer IV neurons. Slice electrophysiology and optogenetics proved that these connections represent functional synapses. In addition, we assessed intracortical activation via immediate-early-gene expression resulting from a behavioral exploration task. The cellular composition of activated neuronal ensembles suggests extensive similarities in intracolumnar information processing in the wild-type and reeler brains. We conclude that extensive ectopic positioning of neuronal partners can be compensated for by cell-autonomous mechanisms that allow for the establishment of proper connectivity. Thus, genetic neuronal fate seems to be of greater importance for correct cortical wiring than radial neuronal position."],["dc.identifier.doi","10.1093/cercor/bhv257"],["dc.identifier.isi","000371522500030"],["dc.identifier.pmid","26564256"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14147"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/41460"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press Inc"],["dc.relation.issn","1460-2199"],["dc.relation.issn","1047-3211"],["dc.rights","CC BY-NC 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc/4.0"],["dc.title","Thalamocortical Connections Drive Intracortical Activation of Functional Columns in the Mislaminated Reeler Somatosensory Cortex"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3450"],["dc.bibliographiccitation.issue","13"],["dc.bibliographiccitation.journal","Cell Reports"],["dc.bibliographiccitation.lastpage","3461.e8"],["dc.bibliographiccitation.volume","28"],["dc.contributor.author","Hafner, Georg"],["dc.contributor.author","Witte, Mirko"],["dc.contributor.author","Guy, Julien"],["dc.contributor.author","Subhashini, Nidhi"],["dc.contributor.author","Fenno, Lief E."],["dc.contributor.author","Ramakrishnan, Charu"],["dc.contributor.author","Kim, Yoon Seok"],["dc.contributor.author","Deisseroth, Karl"],["dc.contributor.author","Callaway, Edward M."],["dc.contributor.author","Oberhuber, Martina"],["dc.contributor.author","Conzelmann, Karl-Klaus"],["dc.contributor.author","Staiger, Jochen F."],["dc.date.accessioned","2020-12-10T14:23:02Z"],["dc.date.available","2020-12-10T14:23:02Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1016/j.celrep.2019.08.064"],["dc.identifier.issn","2211-1247"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16830"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/71810"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.intern","Merged from goescholar"],["dc.rights","CC BY-NC-ND 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc-nd/4.0"],["dc.title","Mapping Brain-Wide Afferent Inputs of Parvalbumin-Expressing GABAergic Neurons in Barrel Cortex Reveals Local and Long-Range Circuit Motifs"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]