Liesegang, Heiko

[["dc.bibliographiccitation.firstpage","760"],["dc.bibliographiccitation.journal","Microbiology"],["dc.bibliographiccitation.lastpage","773"],["dc.bibliographiccitation.volume","157"],["dc.contributor.author","Rechnitzer, Hagai"],["dc.contributor.author","Brzuszkiewicz, Elzbieta B."],["dc.contributor.author","Strittmatter, Axel W."],["dc.contributor.author","Liesegang, Heiko"],["dc.contributor.author","Lysnyansky, Inna"],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Gottschalk, Gerhard"],["dc.contributor.author","Rottem, Shlomo"],["dc.date.accessioned","2018-11-07T08:58:29Z"],["dc.date.available","2018-11-07T08:58:29Z"],["dc.date.issued","2011"],["dc.description.abstract","We present the complete genomic sequence of Mycoplasma fermentans, an organism suggested to be associated with the pathogenesis of rheumatoid arthritis in humans. The genome is composed of 977 524 bp and has a mean G + C content of 26.95 mol%. There are 835 predicted protein-coding sequences and a mean coding density of 87.6%. Functions have been assigned to 58.8% of the predicted protein-coding sequences, while 18.4% of the proteins are conserved hypothetical proteins and 22.8% are hypothetical proteins. In addition, there are two complete rRNA operons and 36 tRNA coding sequences. The largest gene families are the ABC transporter family (42 members), and the functionally heterogeneous group of lipoproteins (28 members), which encode the characteristic prokaryotic cysteine 'lipobox'. Protein secretion occurs through a pathway consisting of SecA, SecD, SecE, SecG, SecY and YidC. Some highly conserved eubacterial proteins, such as GroEL and GroES, are notably absent. The genes encoding DnaK-DnaJ-GrpE and Tig, forming the putative complex of chaperones, are intact, providing the only known control over protein folding. Eighteen nucleases and 17 proteases and peptidases were detected as well as three genes for the thioredoxin-thioreductase system. Overall, this study presents insights into the physiology of M. fermentans, and provides several examples of the genetic basis of systems that might function as virulence factors in this organism."],["dc.description.sponsorship","FEMS; Niedersachsisches Ministerium fur Wissenschaft und Kultur"],["dc.identifier.doi","10.1099/mic.0.043208-0"],["dc.identifier.isi","000288833000015"],["dc.identifier.pmid","21109561"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/23653"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Soc General Microbiology"],["dc.relation.issn","1350-0872"],["dc.title","Genomic features and insights into the biology of Mycoplasma fermentans"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","91"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Journal of Antimicrobial Chemotherapy"],["dc.bibliographiccitation.lastpage","100"],["dc.bibliographiccitation.volume","67"],["dc.contributor.author","Michael, Geovana Brenner"],["dc.contributor.author","Kadlec, Kristina"],["dc.contributor.author","Sweeney, Michael T."],["dc.contributor.author","Brzuszkiewicz, Elzbieta B."],["dc.contributor.author","Liesegang, Heiko"],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Murray, Robert W."],["dc.contributor.author","Watts, Jeffrey L."],["dc.contributor.author","Schwarz, Stephan K. W."],["dc.date.accessioned","2018-11-07T09:15:37Z"],["dc.date.available","2018-11-07T09:15:37Z"],["dc.date.issued","2012"],["dc.description.abstract","Background: Integrative and conjugative elements (ICEs) have not been detected in Pasteurella multocida. In this study the multiresistance ICEPmu1 from bovine P. multocida was analysed for its core genes and its ability to conjugatively transfer into strains of the same and different genera. Methods: ICEPmu1 was identified during whole genome sequencing. Coding sequences were predicted by bioinformatic tools and manually curated using the annotation software ERGO. Conjugation into P. multocida, Mannheimia haemolytica and Escherichia coli recipients was performed by mating assays. The presence of ICEPmu1 and its circular intermediate in the recipient strains was confirmed by PCR and sequence analysis. Integration sites were sequenced. Susceptibility testing of the ICEPmu1-carrying recipients was conducted by broth microdilution. Results: The 82214 bp ICEPmu1 harbours 88 genes. The core genes of ICEPmu1, which are involved in excision/ integration and conjugative transfer, resemble those found in a 66641 bp ICE from Histophilus somni. ICEPmu1 integrates into a tRNA(Leu) and is flanked by 13 bp direct repeats. It is able to conjugatively transfer to P. multocida, M. haemolytica and E. coli, where it also uses a tRNA(Leu) for integration and produces closely related 13 bp direct repeats. PCR assays and susceptibility testing confirmed the presence and the functional activity of the ICEPmu1-associated resistance genes in the recipient strains. Conclusions: The observation that the multiresistance ICEPmu1 is present in a bovine P. multocida and can easily spread across strain and genus boundaries underlines the risk of a rapid dissemination of multiple resistance genes, which will distinctly decrease the therapeutic options."],["dc.identifier.doi","10.1093/jac/dkr411"],["dc.identifier.isi","000300833700014"],["dc.identifier.pmid","22001176"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/27735"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press"],["dc.relation.issn","0305-7453"],["dc.title","ICEPmu1, an integrative conjugative element (ICE) of Pasteurella multocida: structure and transfer"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2475"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Antimicrobial Agents and Chemotherapy"],["dc.bibliographiccitation.lastpage","2477"],["dc.bibliographiccitation.volume","55"],["dc.contributor.author","Kadlec, Kristina"],["dc.contributor.author","Michael, Geovana Brenner"],["dc.contributor.author","Sweeney, Michael T."],["dc.contributor.author","Brzuszkiewicz, Elzbieta B."],["dc.contributor.author","Liesegang, Heiko"],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Watts, Jeffrey L."],["dc.contributor.author","Schwarz, Stephan K. W."],["dc.date.accessioned","2018-11-07T08:56:29Z"],["dc.date.available","2018-11-07T08:56:29Z"],["dc.date.issued","2011"],["dc.description.abstract","The mechanism of macrolide-triamilide resistance in Pasteurella multocida has been unknown. During whole-genome sequencing of a multiresistant bovine P. multocida isolate, three new resistance genes, the rRNA methylase gene erm(42), the macrolide transporter gene msr(E), and the macrolide phosphotransferase gene mph(E), were detected. The three genes were PCR amplified, cloned into suitable plasmid vectors, and shown to confer either macrolide-lincosamide resistance [erm(42)] or macrolide-triamilide resistance [msr(E)=mph(E)] in macrolide-susceptible Escherichia coli and P. multocida hosts."],["dc.identifier.doi","10.1128/AAC.00092-11"],["dc.identifier.isi","000290019200094"],["dc.identifier.pmid","21402855"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/23167"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Soc Microbiology"],["dc.relation.issn","0066-4804"],["dc.title","Molecular Basis of Macrolide, Triamilide, and Lincosamide Resistance in Pasteurella multocida from Bovine Respiratory Disease"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","12879"],["dc.bibliographiccitation.issue","34"],["dc.bibliographiccitation.journal","PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"],["dc.bibliographiccitation.lastpage","12884"],["dc.bibliographiccitation.volume","103"],["dc.contributor.author","Brzuszkiewicz, Elzbieta B."],["dc.contributor.author","Brüggemann, Holger"],["dc.contributor.author","Liesegang, Heiko"],["dc.contributor.author","Emmerth, Melanie"],["dc.contributor.author","Ölschläger, Tobias"],["dc.contributor.author","Nagy, Gábor"],["dc.contributor.author","Albermann, Kaj"],["dc.contributor.author","Wagner, Christian"],["dc.contributor.author","Buchrieser, Carmen"],["dc.contributor.author","Emődy, Levente"],["dc.contributor.author","Gottschalk, Gerhard"],["dc.contributor.author","Hacker, Jörg"],["dc.contributor.author","Dobrindt, Ulrich"],["dc.date.accessioned","2018-11-07T09:25:07Z"],["dc.date.available","2018-11-07T09:25:07Z"],["dc.date.issued","2006"],["dc.description.abstract","Uropathogenic Escherichia coli (UPEC) strain 536 (O6:K15:H31) is one of the model organisms of extraintestinal pathogenic E. coli (ExPEC). To analyze this strain's genetic basis of urovirulence, we sequenced the entire genome and compared the data with the genome sequence of UPEC strain CFT073 (O6:K2:H1) and to the available genomes of nonpathogenic E. coli strain MG1655 (K-12) and enterohemorrhagic E. coli. The genome of strain 536 is approximate to 292 kb smaller than that of strain CFT073. Genomic differences between both UPEC are mainly restricted to large pathogenicity islands, parts of which are unique to strain 536 or CFT073. Genome comparison underlines that repeated insertions and deletions in certain parts of the genome contribute to genome evolution. Furthermore, 427 and 432 genes are only present in strain 536 or in both UPEC, respectively. The majority of the latter genes is encoded within smaller horizontally acquired DNA regions scattered all over the genome. Several of these genes are involved in increasing the pathogens' fitness and adaptability. Analysis of virulence-associated traits expressed in the two UPEC 06 strains, together with genome comparison, demonstrate the marked genetic and phenotypic variability among UPEC. The ability to accumulate and express a variety of virulence-associated genes distinguishes ExPEC from many commensals and forms the basis for the individual virulence potential of ExPEC. Accordingly, instead of a common virulence mechanism, different ways exist among ExPEC to cause disease."],["dc.identifier.doi","10.1073/pnas.0603038103"],["dc.identifier.isi","000240035900043"],["dc.identifier.pmid","16912116"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/29991"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.notes.submitter","Najko"],["dc.relation.issn","0027-8424"],["dc.title","How to become a uropathogen: Comparative genomic analysis of extraintestinal pathogenic Escherichia coli strains"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","84"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Journal of Antimicrobial Chemotherapy"],["dc.bibliographiccitation.lastpage","90"],["dc.bibliographiccitation.volume","67"],["dc.contributor.author","Michael, Geovana Brenner"],["dc.contributor.author","Kadlec, Kristina"],["dc.contributor.author","Sweeney, Michael T."],["dc.contributor.author","Brzuszkiewicz, Elzbieta B."],["dc.contributor.author","Liesegang, Heiko"],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Murray, Robert W."],["dc.contributor.author","Watts, Jeffrey L."],["dc.contributor.author","Schwarz, Stephan K. W."],["dc.date.accessioned","2018-11-07T09:15:36Z"],["dc.date.available","2018-11-07T09:15:36Z"],["dc.date.issued","2012"],["dc.description.abstract","Background: In recent years, multiresistant Pasteurella multocida isolates from bovine respiratory tract infections have been identified. These isolates have exhibited resistance to most classes of antimicrobial agents commonly used in veterinary medicine, the genetic basis of which, however, is largely unknown. Methods: Genomic DNA of a representative P. multocida isolate was subjected to whole genome sequencing. Genes have been predicted by the YACOP program, compared with the SWISSProt/EMBL databases and manually curated using the annotation software ERGO. Susceptibility testingwas performed by broth microdilution according to CLSI recommendations. Results: The analysis of one representative P. multocida isolate identified an 82 kb integrative and conjugative element (ICE) integrated into the chromosomal DNA. This ICE, designated ICEPmu1, harboured 11 resistance genes, which confer resistance to streptomycin/spectinomycin (aadA25), streptomycin (strA and strB), gentamicin (aadB), kanamycin/neomycin (aphA1), tetracycline [tetR-tet(H)], chloramphenicol/florfenicol (floR), sulphonamides (sul2), tilmicosin/clindamycin [erm(42)] or tilmicosin/tulathromycin [msr(E)-mph(E)]. In addition, a complete bla(OXA-2) gene was detected, which, however, appeared to be functionally inactive in P. multocida. These resistance genes were organized in two regions of approximately 15.7 and 9.8 kb. Based on the sequences obtained, it is likely that plasmids, gene cassettes and insertion sequences have played a role in the development of the two resistance gene regions within this ICE. Conclusions: The observation that 12 resistance genes, organized in two resistance gene regions, represent part of an ICE in P. multocida underlines the risk of simultaneous acquisition of multiple resistance genes via a single horizontal gene transfer event."],["dc.identifier.doi","10.1093/jac/dkr406"],["dc.identifier.isi","000300833700013"],["dc.identifier.pmid","22001175"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/27734"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press"],["dc.relation.issn","0305-7453"],["dc.title","ICEPmu1, an integrative conjugative element (ICE) of Pasteurella multocida: analysis of the regions that comprise 12 antimicrobial resistance genes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]