Liesegang, Heiko

[["dc.bibliographiccitation.firstpage","883"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","Archives of Microbiology"],["dc.bibliographiccitation.lastpage","891"],["dc.bibliographiccitation.volume","193"],["dc.contributor.author","Brzuszkiewicz, Elzbieta B."],["dc.contributor.author","Thuermer, Andrea"],["dc.contributor.author","Schuldes, Joerg"],["dc.contributor.author","Leimbach, Andreas"],["dc.contributor.author","Liesegang, Heiko"],["dc.contributor.author","Meyer, Frauke-Dorothee"],["dc.contributor.author","Boelter, Juergen"],["dc.contributor.author","Petersen, Heiko"],["dc.contributor.author","Gottschalk, Gerhard"],["dc.contributor.author","Daniel, Rolf"],["dc.date.accessioned","2018-11-07T08:49:30Z"],["dc.date.available","2018-11-07T08:49:30Z"],["dc.date.issued","2011"],["dc.description.abstract","The genome sequences of two Escherichia coli O104:H4 strains derived from two different patients of the 2011 German E. coli outbreak were determined. The two analyzed strains were designated E. coli GOS1 and GOS2 (German outbreak strain). Both isolates comprise one chromosome of approximately 5.31 Mbp and two putative plasmids. Comparisons of the 5,217 (GOS1) and 5,224 (GOS2) predicted protein-encoding genes with various E. coli strains, and a multilocus sequence typing analysis revealed that the isolates were most similar to the entero-aggregative E. coli (EAEC) strain 55989. In addition, one of the putative plasmids of the outbreak strain is similar to pAA-type plasmids of EAEC strains, which contain aggregative adhesion fimbrial operons. The second putative plasmid harbors genes for extended-spectrum beta-lactamases. This type of plasmid is widely distributed in pathogenic E. coli strains. A significant difference of the E. coli GOS1 and GOS2 genomes to those of EAEC strains is the presence of a prophage encoding the Shiga toxin, which is characteristic for enterohemorrhagic E. coli (EHEC) strains. The unique combination of genomic features of the German outbreak strain, containing characteristics from pathotypes EAEC and EHEC, suggested that it represents a new pathotype Entero-Aggregative-Haemorrhagic Escherichia coli (EAHEC)."],["dc.identifier.doi","10.1007/s00203-011-0725-6"],["dc.identifier.isi","000297223500005"],["dc.identifier.pmid","21713444"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7515"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/21476"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","0302-8933"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Genome sequence analyses of two isolates from the recent Escherichia coli outbreak in Germany reveal the emergence of a new pathotype: Entero-Aggregative-Haemorrhagic Escherichia coli (EAHEC)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","BMC Genomics"],["dc.bibliographiccitation.lastpage","14"],["dc.bibliographiccitation.volume","19"],["dc.contributor.author","Groß, Uwe"],["dc.contributor.author","Brzuszkiewicz, Elzbieta B."],["dc.contributor.author","Gunka, Katrin"],["dc.contributor.author","Starke, Jessica"],["dc.contributor.author","Riedel, Thomas"],["dc.contributor.author","Bunk, Boyke"],["dc.contributor.author","Spröer, Cathrin"],["dc.contributor.author","Wetzel, Daniela"],["dc.contributor.author","Poehlein, Anja"],["dc.contributor.author","Chibani, Cynthia"],["dc.contributor.author","Bohne, Wolfgang"],["dc.contributor.author","Overmann, Jörg"],["dc.contributor.author","Zimmermann, Ortrud"],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Liesegang, Heiko"],["dc.date.accessioned","2019-07-09T11:45:11Z"],["dc.date.available","2019-07-09T11:45:11Z"],["dc.date.issued","2018"],["dc.description.abstract","BACKGROUND: Clostridioides difficile infections (CDI) have emerged over the past decade causing symptoms that range from mild, antibiotic-associated diarrhea (AAD) to life-threatening toxic megacolon. In this study, we describe a multiple and isochronal (mixed) CDI caused by the isolates DSM 27638, DSM 27639 and DSM 27640 that already initially showed different morphotypes on solid media. RESULTS: The three isolates belonging to the ribotypes (RT) 012 (DSM 27639) and 027 (DSM 27638 and DSM 27640) were phenotypically characterized and high quality closed genome sequences were generated. The genomes were compared with seven reference strains including three strains of the RT 027, two of the RT 017, and one of the RT 078 as well as a multi-resistant RT 012 strain. The analysis of horizontal gene transfer events revealed gene acquisition incidents that sort the strains within the time line of the spread of their RTs within Germany. We could show as well that horizontal gene transfer between the members of different RTs occurred within this multiple infection. In addition, acquisition and exchange of virulence-related features including antibiotic resistance genes were observed. Analysis of the two genomes assigned to RT 027 revealed three single nucleotide polymorphisms (SNPs) and apparently a regional genome modification within the flagellar switch that regulates the fli operon. CONCLUSION: Our findings show that (i) evolutionary events based on horizontal gene transfer occur within an ongoing CDI and contribute to the adaptation of the species by the introduction of new genes into the genomes, (ii) within a multiple infection of a single patient the exchange of genetic material was responsible for a much higher genome variation than the observed SNPs."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2018"],["dc.identifier.doi","10.1186/s12864-017-4368-0"],["dc.identifier.pmid","29291715"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15054"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59178"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.notes.intern","In goescholar not merged with http://resolver.sub.uni-goettingen.de/purl?gs-1/15123 but duplicate"],["dc.notes.status","final"],["dc.relation.issn","1471-2164"],["dc.rights","CC BY 4.0"],["dc.rights.access","openAccess"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","570"],["dc.title","Comparative genome and phenotypic analysis of three Clostridioides difficile strains isolated from a single patient provide insight into multiple infection of C. difficile."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e1001078"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","PLoS Pathogens"],["dc.bibliographiccitation.volume","6"],["dc.contributor.author","Zdziarski, Jaroslaw"],["dc.contributor.author","Brzuszkiewicz, Elzbieta B."],["dc.contributor.author","Wullt, Bjorn"],["dc.contributor.author","Liesegang, Heiko"],["dc.contributor.author","Biran, Dvora"],["dc.contributor.author","Voigt, Birgit"],["dc.contributor.author","Gronberg-Hernandez, Jenny"],["dc.contributor.author","Ragnarsdottir, Bryndis"],["dc.contributor.author","Hecker, Michael"],["dc.contributor.author","Ron, Eliora Z."],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Gottschalk, Gerhard"],["dc.contributor.author","Hacker, Joerg"],["dc.contributor.author","Svanborg, Catharina"],["dc.contributor.author","Dobrindt, Ulrich"],["dc.date.accessioned","2018-11-07T08:41:04Z"],["dc.date.available","2018-11-07T08:41:04Z"],["dc.date.issued","2010"],["dc.description.abstract","Bacteria lose or gain genetic material and through selection, new variants become fixed in the population. Here we provide the first, genome-wide example of a single bacterial strain's evolution in different deliberately colonized patients and the surprising insight that hosts appear to personalize their microflora. By first obtaining the complete genome sequence of the prototype asymptomatic bacteriuria strain E. coli 83972 and then resequencing its descendants after therapeutic bladder colonization of different patients, we identified 34 mutations, which affected metabolic and virulence-related genes. Further transcriptome and proteome analysis proved that these genome changes altered bacterial gene expression resulting in unique adaptation patterns in each patient. Our results provide evidence that, in addition to stochastic events, adaptive bacterial evolution is driven by individual host environments. Ongoing loss of gene function supports the hypothesis that evolution towards commensalism rather than virulence is favored during asymptomatic bladder colonization."],["dc.identifier.doi","10.1371/journal.ppat.1001078"],["dc.identifier.isi","000281399900048"],["dc.identifier.pmid","20865122"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7263"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/19388"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1553-7366"],["dc.rights","CC BY 2.5"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.5"],["dc.title","Host Imprints on Bacterial Genomes-Rapid, Divergent Evolution in Individual Patients"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","577"],["dc.bibliographiccitation.journal","BMC Genomics"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Gounder, Kamini"],["dc.contributor.author","Brzuszkiewicz, Elzbieta B."],["dc.contributor.author","Liesegang, Heiko"],["dc.contributor.author","Wollherr, Antje"],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Gottschalk, Gerhard"],["dc.contributor.author","Reva, Oleg"],["dc.contributor.author","Kumwenda, Benjamin"],["dc.contributor.author","Srivastava, Malay"],["dc.contributor.author","Bricio, Carlos"],["dc.contributor.author","Berenguer, Jose"],["dc.contributor.author","van Heerden, Esta"],["dc.contributor.author","Litthauer, Derek"],["dc.date.accessioned","2018-11-07T08:49:39Z"],["dc.date.available","2018-11-07T08:49:39Z"],["dc.date.issued","2011"],["dc.description.abstract","Background: Many strains of Thermus have been isolated from hot environments around the world. Thermus scotoductus SA-01 was isolated from fissure water collected 3.2 km below surface in a South African gold mine. The isolate is capable of dissimilatory iron reduction, growth with oxygen and nitrate as terminal electron acceptors and the ability to reduce a variety of metal ions, including gold, chromate and uranium, was demonstrated. The genomes from two different Thermus thermophilus strains have been completed. This paper represents the completed genome from a second Thermus species - T. scotoductus. Results: The genome of Thermus scotoductus SA-01 consists of a chromosome of 2,346,803 bp and a small plasmid which, together are about 11% larger than the Thermus thermophilus genomes. The T. thermophilus megaplasmid genes are part of the T. scotoductus chromosome and extensive rearrangement, deletion of nonessential genes and acquisition of gene islands have occurred, leading to a loss of synteny between the chromosomes of T. scotoductus and T. thermophilus. At least nine large inserts of which seven were identified as alien, were found, the most remarkable being a denitrification cluster and two operons relating to the metabolism of phenolics which appear to have been acquired from Meiothermus ruber. The majority of acquired genes are from closely related species of the Deinococcus-Thermus group, and many of the remaining genes are from microorganisms with a thermophilic or hyperthermophilic lifestyle. The natural competence of Thermus scotoductus was confirmed experimentally as expected as most of the proteins of the natural transformation system of Thermus thermophilus are present. Analysis of the metabolic capabilities revealed an extensive energy metabolism with many aerobic and anaerobic respiratory options. An abundance of sensor histidine kinases, response regulators and transporters for a wide variety of compounds are indicative of an oligotrophic lifestyle. Conclusions: The genome of Thermus scotoductus SA-01 shows remarkable plasticity with the loss, acquisition and rearrangement of large portions of its genome compared to Thermus thermophilus. Its ability to naturally take up foreign DNA has helped it adapt rapidly to a subsurface lifestyle in the presence of a dense and diverse population which acted as source of nutrients. The genome of Thermus scotoductus illustrates how rapid adaptation can be achieved by a highly dynamic and plastic genome."],["dc.identifier.doi","10.1186/1471-2164-12-577"],["dc.identifier.isi","000297856200002"],["dc.identifier.pmid","22115438"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7032"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/21516"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1471-2164"],["dc.rights","CC BY 2.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.0"],["dc.title","Sequence of the hyperplastic genome of the naturally competent Thermus scotoductus SA-01"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e1002169"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","PLoS Biology"],["dc.bibliographiccitation.volume","13"],["dc.contributor.author","Masri, Leila"],["dc.contributor.author","Branca, Antoine"],["dc.contributor.author","Sheppard, Anna E."],["dc.contributor.author","Papkou, Andrei"],["dc.contributor.author","Laehnemann, David"],["dc.contributor.author","Guenther, Patrick S."],["dc.contributor.author","Prahl, Swantje"],["dc.contributor.author","Saebelfeld, Manja"],["dc.contributor.author","Hollensteiner, Jacqueline"],["dc.contributor.author","Liesegang, Heiko"],["dc.contributor.author","Brzuszkiewicz, Elzbieta B."],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Michiels, Nicolaas K."],["dc.contributor.author","Schulte, Rebecca D."],["dc.contributor.author","Kurtz, Joachim"],["dc.contributor.author","Rosenstiel, Philip"],["dc.contributor.author","Telschow, Arndt"],["dc.contributor.author","Bornberg-Bauer, Erich"],["dc.contributor.author","Schulenburg, Hinrich"],["dc.date.accessioned","2018-11-07T09:56:21Z"],["dc.date.available","2018-11-07T09:56:21Z"],["dc.date.issued","2015"],["dc.description.abstract","Reciprocal coevolution between host and pathogen is widely seen as a major driver of evolution and biological innovation. Yet, to date, the underlying genetic mechanisms and associated trait functions that are unique to rapid coevolutionary change are generally unknown. We here combined experimental evolution of the bacterial biocontrol agent Bacillus thuringiensis and its nematode host Caenorhabditis elegans with large-scale phenotyping, whole genome analysis, and functional genetics to demonstrate the selective benefit of pathogen virulence and the underlying toxin genes during the adaptation process. We show that: (i) high virulence was specifically favoured during pathogen-host coevolution rather than pathogen one-sided adaptation to a nonchanging host or to an environment without host; (ii) the pathogen genotype BT-679 with known nematocidal toxin genes and high virulence specifically swept to fixation in all of the independent replicate populations under coevolution but only some under one-sided adaptation; (iii) high virulence in the BT-679-dominated populations correlated with elevated copy numbers of the plasmid containing the nematocidal toxin genes; (iv) loss of virulence in a toxin-plasmid lacking BT-679 isolate was reconstituted by genetic reintroduction or external addition of the toxins. We conclude that sustained coevolution is distinct from unidirectional selection in shaping the pathogen's genome and life history characteristics. To our knowledge, this study is the first to characterize the pathogen genes involved in coevolutionary adaptation in an animal host-pathogen interaction system."],["dc.identifier.doi","10.1371/journal.pbio.1002169"],["dc.identifier.isi","000357339600009"],["dc.identifier.pmid","26042786"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/11996"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/36941"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1545-7885"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Host-Pathogen Coevolution: The Selective Advantage of Bacillus thuringiensis Virulence and Its Cry Toxin Genes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e90914"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Djukic, Marvin"],["dc.contributor.author","Brzuszkiewicz, Elzbieta B."],["dc.contributor.author","Fünfhaus, Anne"],["dc.contributor.author","Voss, Jörn"],["dc.contributor.author","Gollnow, Kathleen"],["dc.contributor.author","Poppinga, Lena"],["dc.contributor.author","Liesegang, Heiko"],["dc.contributor.author","Garcia-Gonzalez, Eva"],["dc.contributor.author","Genersch, Elke"],["dc.contributor.author","Daniel, Rolf"],["dc.date.accessioned","2019-07-09T11:39:38Z"],["dc.date.available","2019-07-09T11:39:38Z"],["dc.date.issued","2014"],["dc.description.abstract","Paenibacillus larvae, a Gram positive bacterial pathogen, causes American Foulbrood (AFB), which is the most serious infectious disease of honey bees. In order to investigate the genomic potential of P. larvae, two strains belonging to two different genotypes were sequenced and used for comparative genome analysis. The complete genome sequence of P. larvae strain DSM 25430 (genotype ERIC II) consisted of 4,056,006 bp and harbored 3,928 predicted protein-encoding genes. The draft genome sequence of P. larvae strain DSM 25719 (genotype ERIC I) comprised 4,579,589 bp and contained 4,868 protein-encoding genes. Both strains harbored a 9.7 kb plasmid and encoded a large number of virulence-associated proteins such as toxins and collagenases. In addition, genes encoding large multimodular enzymes producing nonribosomally peptides or polyketides were identified. In the genome of strain DSM 25719 seven toxin associated loci were identified and analyzed. Five of them encoded putatively functional toxins. The genome of strain DSM 25430 harbored several toxin loci that showed similarity to corresponding loci in the genome of strain DSM 25719, but were non-functional due to point mutations or disruption by transposases. Although both strains cause AFB, significant differences between the genomes were observed including genome size, number and composition of transposases, insertion elements, predicted phage regions, and strain-specific island-like regions. Transposases, integrases and recombinases are important drivers for genome plasticity. A total of 390 and 273 mobile elements were found in strain DSM 25430 and strain DSM 25719, respectively. Comparative genomics of both strains revealed acquisition of virulence factors by horizontal gene transfer and provided insights into evolution and pathogenicity."],["dc.identifier.doi","10.1371/journal.pone.0090914"],["dc.identifier.fs","604744"],["dc.identifier.pmid","24599066"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/10019"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/58017"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","How to Kill the Honey Bee Larva: Genomic Potential and Virulence Mechanisms of Paenibacillus larvae"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e10986"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Klee, Silke R."],["dc.contributor.author","Brzuszkiewicz, Elzbieta B."],["dc.contributor.author","Nattermann, Herbert"],["dc.contributor.author","Brueggemann, Holger"],["dc.contributor.author","Dupke, Susann"],["dc.contributor.author","Wollherr, Antje"],["dc.contributor.author","Franz, Tatjana"],["dc.contributor.author","Pauli, Georg"],["dc.contributor.author","Appel, Bernd"],["dc.contributor.author","Liebl, Wolfgang"],["dc.contributor.author","Couacy-Hymann, Emmanuel"],["dc.contributor.author","Boesch, Christophe"],["dc.contributor.author","Meyer, Frauke-Dorothee"],["dc.contributor.author","Leendertz, Fabian H."],["dc.contributor.author","Ellerbrok, Heinz"],["dc.contributor.author","Gottschalk, Gerhard"],["dc.contributor.author","Grunow, Roland"],["dc.contributor.author","Liesegang, Heiko"],["dc.date.accessioned","2018-11-07T08:41:23Z"],["dc.date.available","2018-11-07T08:41:23Z"],["dc.date.issued","2010"],["dc.description.abstract","Anthrax is a fatal disease caused by strains of Bacillus anthracis. Members of this monophyletic species are non motile and are all characterized by the presence of four prophages and a nonsense mutation in the plcR regulator gene. Here we report the complete genome sequence of a Bacillus strain isolated from a chimpanzee that had died with clinical symptoms of anthrax. Unlike classic B. anthracis, this strain was motile and lacked the four prohages and the nonsense mutation. Four replicons were identified, a chromosome and three plasmids. Comparative genome analysis revealed that the chromosome resembles those of non-B. anthracis members of the Bacillus cereus group, whereas two plasmids were identical to the anthrax virulence plasmids pXO1 and pXO2. The function of the newly discovered third plasmid with a length of 14 kbp is unknown. A detailed comparison of genomic loci encoding key features confirmed a higher similarity to B. thuringiensis serovar konkukian strain 97-27 and B. cereus E33L than to B. anthracis strains. For the first time we describe the sequence of an anthrax causing bacterium possessing both anthrax plasmids that apparently does not belong to the monophyletic group of all so far known B. anthracis strains and that differs in important diagnostic features. The data suggest that this bacterium has evolved from a B. cereus strain independently from the classic B. anthracis strains and established a B. anthracis lifestyle. Therefore we suggest to designate this isolate as \"B. cereus variety (var.) anthracis'."],["dc.identifier.doi","10.1371/journal.pone.0010986"],["dc.identifier.isi","000279715300001"],["dc.identifier.pmid","20634886"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7275"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/19459"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 2.5"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.5"],["dc.title","The Genome of a Bacillus Isolate Causing Anthrax in Chimpanzees Combines Chromosomal Properties of B. cereus with B. anthracis Virulence Plasmids"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","2171"],["dc.bibliographiccitation.journal","Frontiers in Microbiology"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Hollensteiner, Jacqueline"],["dc.contributor.author","Wemheuer, Franziska"],["dc.contributor.author","Harting, Rebekka"],["dc.contributor.author","Kolarzyk, Anna M."],["dc.contributor.author","Valeria, Stefani M. Diaz"],["dc.contributor.author","Poehlein, Anja"],["dc.contributor.author","Brzuszkiewicz, Elzbieta B."],["dc.contributor.author","Nesemann, Kai"],["dc.contributor.author","Braus-Stromeyer, Susanna A."],["dc.contributor.author","Braus, Gerhard H."],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Liesegang, Heiko"],["dc.date.accessioned","2018-11-07T10:28:22Z"],["dc.date.available","2018-11-07T10:28:22Z"],["dc.date.issued","2017"],["dc.description.abstract","Verticillium wilt causes severe yield losses in a broad range of economically important crops worldwide. As many soil fumigants have a severe environmental impact, new biocontrol strategies are needed. Members of the genus Bacillus are known as plant growth-promoting bacteria (PGPB) as well as biocontrol agents of pests and diseases. In this study, we isolated 267 Bacillus strains from root-associated soil of field-grown tomato plants. We evaluated the antifungal potential of 20 phenotypically diverse strains according to their antagonistic activity against the two phytopathogenic fungi Verticillium dahliae and Verticillium longisporum. In addition, the 20 strains were sequenced and phylogenetically characterized by multi-locus sequence typing (MLST) resulting in 7 different Bacillus thuringiensis and 13 Bacillus weihenstephanensis strains. All B. thuringiensis isolates inhibited in vitro the tomato pathogen V dahliae JR2, but had only low efficacy against the tomato foreign pathogen V longisporum 43. All B. weihenstephanensis isolates exhibited no fungicidal activity whereas three B. weihenstephanensis isolates showed antagonistic effects on both phytopathogens. These strains had a rhizoid colony morphology, which has not been described for B. weihenstephanensis strains previously. Genome analysis of all isolates revealed putative genes encoding fungicidal substances and resulted in identification of 304 secondary metabolite gene clusters including 101 non-ribosomal polypeptide synthetases and 203 ribosomal-synthesized and post-translationally modified peptides. All genomes encoded genes for the synthesis of the antifungal siderophore bacillibactin. In the genome of one B. thuringiensis strain, a gene cluster for zwittermicin A was detected. Isolates which either exhibited an inhibitory or an interfering effect on the growth of the phytopathogens carried one or two genes encoding putative mycolitic chitinases, which might contribute to antifungal activities. This indicates that chitinases contribute to antifungal activities. The present study identified B. thuringiensis isolates from tomato roots which exhibited in vitro antifungal activity against Verticillium species."],["dc.description.sponsorship","German Research Foundation [DFG-SPP1399, LI 1690/2-1, DFG BR1502/15-1]"],["dc.identifier.doi","10.3389/fmicb.2016.02171"],["dc.identifier.isi","000392130500001"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14239"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/43404"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1664-302X"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Bacillus thuringiensis and Bacillus weihenstephanensis Inhibit the Growth of Phytopathogenic Verticillium Species"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]