Liesegang, Heiko

[["dc.bibliographiccitation.artnumber","5699"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Scientific reports"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Hertel, Robert"],["dc.contributor.author","Meyerjürgens, Sandra"],["dc.contributor.author","Voigt, Birgit"],["dc.contributor.author","Liesegang, Heiko"],["dc.contributor.author","Volland, Sonja"],["dc.date.accessioned","2019-07-09T11:43:27Z"],["dc.date.available","2019-07-09T11:43:27Z"],["dc.date.issued","2017-07-18"],["dc.description.abstract","The species Bacillus licheniformis includes important strains that are used in industrial production processes. Currently the physiological model used to adapt these processes is based on the closely related model organism B. subtilis. In this study we found that both organisms reveal significant differences in the regulation of subtilisin, their main natural protease and a product of industrial fermentation processes. We identified and characterized a novel antisense sRNA AprAs, which represents an RNA based repressor of apr, the gene encoding for the industrial relevant subtilisin protease. Reduction of the AprAs level leads to an enhanced proteolytic activity and an increase of Apr protein expression in the mutant strain. A vector based complementation of the AprAs deficient mutant confirmed this effect and demonstrated the necessity of cis transcription for full efficiency. A comparative analysis of the corresponding genome loci from B. licheniformis and B. subtilis revealed the absence of an aprAs promoter in B. subtilis and indicates that AprAs is a B. licheniformis species specific phenomenon. The discovery of AprAs is of great biotechnological interest since subtilisin Carlsberg is one of the main products of industrial fermentation by B. licheniformis."],["dc.identifier.doi","10.1038/s41598-017-05628-y"],["dc.identifier.pmid","28720814"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14537"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/58891"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","2045-2322"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","570"],["dc.title","Small RNA mediated repression of subtilisin production in Bacillus licheniformis."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","98"],["dc.bibliographiccitation.journal","BMC Evolutionary Biology"],["dc.bibliographiccitation.volume","17"],["dc.contributor.author","Wendling, Carolin C."],["dc.contributor.author","Piecyk, Agnes"],["dc.contributor.author","Refardt, Dominik"],["dc.contributor.author","Chibani, Cynthia"],["dc.contributor.author","Hertel, Robert"],["dc.contributor.author","Liesegang, Heiko"],["dc.contributor.author","Bunk, Boyke"],["dc.contributor.author","Overmann, Joerg"],["dc.contributor.author","Roth, Olivia"],["dc.date.accessioned","2018-11-07T10:25:01Z"],["dc.date.available","2018-11-07T10:25:01Z"],["dc.date.issued","2017"],["dc.description.abstract","Background: Evolutionary shifts in bacterial virulence are often associated with a third biological player, for instance temperate phages, that can act as hyperparasites. By integrating as prophages into the bacterial genome they can contribute accessory genes, which can enhance the fitness of their prokaryotic carrier (lysogenic conversion). Hyperparasitic influence in tripartite biotic interactions has so far been largely neglected in empirical host-parasite studies due to their inherent complexity. Here we experimentally address whether bacterial resistance to phages and bacterial harm to eukaryotic hosts is linked using a natural tri-partite system with bacteria of the genus Vibrio, temperate vibriophages and the pipefish Syngnathus typhle. We induced prophages from all bacterial isolates and constructed a three-fold replicated, fully reciprocal 75 x 75 phage-bacteria infection matrix. Results: According to their resistance to phages, bacteria could be grouped into three distinct categories: highly susceptible (HS-bacteria), intermediate susceptible (IS-bacteria), and resistant (R-bacteria). We experimentally challenged pipefish with three selected bacterial isolates from each of the three categories and determined the amount of viable Vibrio counts from infected pipefish and the expression of pipefish immune genes. While the amount of viable Vibrio counts did not differ between bacterial groups, we observed a significant difference in relative gene expression between pipefish infected with phage susceptible and phage resistant bacteria. Conclusion: These findings suggest that bacteria with a phage-susceptible phenotype are more harmful against a eukaryotic host, and support the importance of hyperparasitism and the need for an integrative view across more than two levels when studying host-parasite evolution."],["dc.identifier.doi","10.1186/s12862-017-0930-2"],["dc.identifier.isi","000399180700001"],["dc.identifier.pmid","28399796"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14411"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/42770"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1471-2148"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Tripartite species interaction: eukaryotic hosts suffer more from phage susceptible than from phage resistant bacteria"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","667"],["dc.bibliographiccitation.journal","BMC Genomics"],["dc.bibliographiccitation.volume","14"],["dc.contributor.author","Wiegand, Sandra"],["dc.contributor.author","Dietrich, Sascha"],["dc.contributor.author","Hertel, Robert"],["dc.contributor.author","Bongaerts, Johannes"],["dc.contributor.author","Evers, Stefan"],["dc.contributor.author","Volland, Sonja"],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Liesegang, Heiko"],["dc.date.accessioned","2018-11-07T09:18:54Z"],["dc.date.available","2018-11-07T09:18:54Z"],["dc.date.issued","2013"],["dc.description.abstract","Background: The production of enzymes by an industrial strain requires a complex adaption of the bacterial metabolism to the conditions within the fermenter. Regulatory events within the process result in a dynamic change of the transcriptional activity of the genome. This complex network of genes is orchestrated by proteins as well as regulatory RNA elements. Here we present an RNA-Seq based study considering selected phases of an industry-oriented fermentation of Bacillus licheniformis. Results: A detailed analysis of 20 strand-specific RNA-Seq datasets revealed a multitude of transcriptionally active genomic regions. 3314 RNA features encoded by such active loci have been identified and sorted into ten functional classes. The identified sequences include the expected RNA features like housekeeping sRNAs, metabolic riboswitches and RNA switches well known from studies on Bacillus subtilis as well as a multitude of completely new candidates for regulatory RNAs. An unexpectedly high number of 855 RNA features are encoded antisense to annotated protein and RNA genes, in addition to 461 independently transcribed small RNAs. These antisense transcripts contain molecules with a remarkable size range variation from 38 to 6348 base pairs in length. The genome of the type strain B. licheniformis DSM13 was completely reannotated using data obtained from RNA-Seq analyses and from public databases. Conclusion: The hereby generated data-sets represent a solid amount of knowledge on the dynamic transcriptional activities during the investigated fermentation stages. The identified regulatory elements enable research on the understanding and the optimization of crucial metabolic activities during a productive fermentation of Bacillus licheniformis strains."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2013"],["dc.identifier.doi","10.1186/1471-2164-14-667"],["dc.identifier.isi","000328628100001"],["dc.identifier.pmid","24079885"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/10046"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/28510"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1471-2164"],["dc.rights","CC BY 2.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.0"],["dc.title","RNA-Seq of Bacillus licheniformis: active regulatory RNA features expressed within a productive fermentation"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e0120759"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Hertel, Robert"],["dc.contributor.author","Rodriguez, David Pintor"],["dc.contributor.author","Hollensteiner, Jacqueline"],["dc.contributor.author","Dietrich, Sascha"],["dc.contributor.author","Leimbach, Andreas"],["dc.contributor.author","Hoppert, Michael"],["dc.contributor.author","Liesegang, Heiko"],["dc.contributor.author","Volland, Sonja"],["dc.date.accessioned","2018-11-07T09:59:27Z"],["dc.date.available","2018-11-07T09:59:27Z"],["dc.date.issued","2015"],["dc.description.abstract","Prophages are viruses, which have integrated their genomes into the genome of a bacterial host. The status of the prophage genome can vary from fully intact with the potential to form infective particles to a remnant state where only a few phage genes persist. Prophages have impact on the properties of their host and are therefore of great interest for genomic research and strain design. Here we present a genome-and next generation sequencing (NGS)-based approach for identification and activity evaluation of prophage regions. Seven prophage or prophage-like regions were identified in the genome of Bacillus licheniformis DSM13. Six of these regions show similarity to members of the Siphoviridae phage family. The remaining region encodes the B. licheniformis orthologue of the PBSX prophage from Bacillus subtilis. Analysis of isolated phage particles (induced by mitomycin C) from the wild-type strain and prophage deletion mutant strains revealed activity of the prophage regions BLi_Pp2 (PBSX-like), BLi_Pp3 and BLi_Pp6. In contrast to BLi_Pp2 and BLi_Pp3, neither phage DNA nor phage particles of BLi_Pp6 could be visualized. However, the ability of prophage BLi_Pp6 to generate particles could be confirmed by sequencing of particle-protected DNA mapping to prophage locus BLi_Pp6. The introduced NGS-based approach allows the investigation of prophage regions and their ability to form particles. Our results show that this approach increases the sensitivity of prophage activity analysis and can complement more conventional approaches such as transmission electron microscopy (TEM)."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2015"],["dc.identifier.doi","10.1371/journal.pone.0120759"],["dc.identifier.isi","000356353700060"],["dc.identifier.pmid","25811873"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/11757"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/37588"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Genome-Based Identification of Active Prophage Regions by Next Generation Sequencing in Bacillus licheniformis DSM13"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1359"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","Viruses"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Chibani, Cynthia Maria"],["dc.contributor.author","Hertel, Robert"],["dc.contributor.author","Hoppert, Michael"],["dc.contributor.author","Liesegang, Heiko"],["dc.contributor.author","Wendling, Carolin Charlotte"],["dc.date.accessioned","2021-04-14T08:31:33Z"],["dc.date.available","2021-04-14T08:31:33Z"],["dc.date.issued","2020"],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft"],["dc.identifier.doi","10.3390/v12121359"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/83635"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.publisher","MDPI"],["dc.relation.eissn","1999-4915"],["dc.rights","https://creativecommons.org/licenses/by/4.0/"],["dc.title","Closely Related Vibrio alginolyticus Strains Encode an Identical Repertoire of Caudovirales-Like Regions and Filamentous Phages"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]