Liesegang, Heiko

[["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Genome Announcements"],["dc.bibliographiccitation.volume","6"],["dc.contributor.author","Wemheuer, Franziska"],["dc.contributor.author","Hollensteiner, Jacqueline"],["dc.contributor.author","Poehlein, Anja"],["dc.contributor.author","Liesegang, Heiko"],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Wemheuer, Bernd"],["dc.date.accessioned","2020-04-28T12:41:35Z"],["dc.date.available","2020-04-28T12:41:35Z"],["dc.date.issued","2018"],["dc.description.abstract","Bacillus mycoides GM6LP is an endophyte isolated from plant tissues of Lolium perenne L. Here, we report its draft genome sequence (6.2 Mb), which contains 96 contigs and 6,129 protein-coding genes. Knowledge about its genome will enable us to evaluate the potential use of GM6LP as a plant growth-promoting bacterium."],["dc.identifier.doi","10.1128/genomeA.00011-18"],["dc.identifier.eissn","2169-8287"],["dc.identifier.pmid","29437086"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/64449"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.relation.issn","2169-8287"],["dc.title","Draft Genome Sequence of the Endophyte Bacillus mycoides Strain GM6LP Isolated from Lolium perenne"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1316"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"],["dc.bibliographiccitation.lastpage","1321"],["dc.bibliographiccitation.volume","100"],["dc.contributor.author","Bruggemann, H."],["dc.contributor.author","Baumer, S."],["dc.contributor.author","Fricke, Wolfgang Florian"],["dc.contributor.author","Wiezer, A."],["dc.contributor.author","Liesegang, Heiko"],["dc.contributor.author","Decker, I."],["dc.contributor.author","Herzberg, C."],["dc.contributor.author","Martinez-Arias, R."],["dc.contributor.author","Merkl, R."],["dc.contributor.author","Henne, A."],["dc.contributor.author","Gottschalk, G."],["dc.date.accessioned","2018-11-07T10:40:58Z"],["dc.date.available","2018-11-07T10:40:58Z"],["dc.date.issued","2003"],["dc.description.abstract","Tetanus disease is one of the most dramatic and globally prevalent diseases of humans and vertebrate animals, and has been reported for over 24 centuries. The manifestation of the disease, spastic paralysis, is caused by the second most poisonous substance known, the tetanus toxin, with a human lethal dose of approximate to1 ng/kg. Fortunately, this disease is successfully controlled through immunization with tetanus toxoid; nevertheless, according to the World Health Organization, an estimated 400,000 cases still occur each year, mainly of neonatal tetanus. The causative agent of tetanus disease is Clostridium tetani, an anaerobic spore-forming bacterium, whose natural habitat is soil, dust, and intestinal tracts of various animals. Here we report the complete genome sequence of toxigenic C. tetani E88, a variant of strain Massachusetts. The genome consists of a 2,799,250-bp chromosome encoding 2,372 ORFs. The tetanus toxin and a collagenase are encoded on a 74,082-bp plasmid, containing 61 ORFs. Additional virulence-related factors could be identified, such as an array of surface-layer and adhesion proteins (35 ORFs), some of them unique to C. tetani. Comparative genomics with the genomes of Clostridium perfringens, the causative agent of gas gangrene, and Clostridium acetobutylicum, a nonpathogenic solvent producer, revealed a remarkable capacity of C. tetani: The organism can rely on an extensive sodium ion bioenergetics. Additional candidate genes involved in the establishment and maintenance of a pathogenic lifestyle of C. tetani are presented."],["dc.identifier.doi","10.1073/pnas.0335853100"],["dc.identifier.isi","000180838100098"],["dc.identifier.pmid","12552129"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/46432"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Natl Acad Sciences"],["dc.relation.issn","0027-8424"],["dc.title","The genome sequence of Clostridium tetani, the causative agent of tetanus disease"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2255"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Applied Microbiology and Biotechnology"],["dc.bibliographiccitation.lastpage","2266"],["dc.bibliographiccitation.volume","99"],["dc.contributor.author","Jakobs, Mareike"],["dc.contributor.author","Hoffmann, Kerstin"],["dc.contributor.author","Liesegang, Heiko"],["dc.contributor.author","Volland, Sonja"],["dc.contributor.author","Meinhardt, Friedhelm"],["dc.date.accessioned","2018-11-07T10:00:29Z"],["dc.date.available","2018-11-07T10:00:29Z"],["dc.date.issued","2015"],["dc.description.abstract","In Bacillus subtilis, natural genetic competence is subject to complex genetic regulation and quorum sensing dependent. Upon extracellular accumulation of the peptide-pheromone ComX, the membrane-bound sensor histidine kinase ComP initiates diverse signaling pathways by activating-among others-DegQ and ComS. While DegQ favors the expression of extracellular enzymes rather than competence development, ComS is crucial for competence development as it prevents proteolytic degradation of ComK, the key transcriptional activator of all genes required for the uptake and integration of DNA. In Bacillus licheniformis, ComX/ComP sensed cell density negatively influences competence development, suggesting differences from the quorum-sensing-dependent control mechanism in Bacillus subtilis. Here, we show that each of six investigated strains possesses both of two different, recently identified putative comS genes. When expressed from an inducible promoter, none of the comS candidate genes displayed an impact on competence development neither in B. subtilis nor in B. licheniformis. Moreover, disruption of the genes did not reduce transformation efficiency. While the putative comS homologs do not contribute to competence development, we provide evidence that the degQ gene as for B. subtilis negatively influences genetic competency in B. licheniformis."],["dc.identifier.doi","10.1007/s00253-014-6291-5"],["dc.identifier.isi","000350029000019"],["dc.identifier.pmid","25520171"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/37819"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","1432-0614"],["dc.relation.issn","0175-7598"],["dc.title","The two putative comS homologs of the biotechnologically important Bacillus licheniformis do not contribute to competence development"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","48"],["dc.bibliographiccitation.journal","Genome Announcements"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Chibani, Cynthia Maria"],["dc.contributor.author","Poehlein, Anja"],["dc.contributor.author","Roth, Olivia"],["dc.contributor.author","Liesegang, Heiko"],["dc.contributor.author","Wendling, Carolin Charlotte"],["dc.date.accessioned","2020-12-10T18:36:58Z"],["dc.date.available","2020-12-10T18:36:58Z"],["dc.date.issued","2017"],["dc.identifier.doi","10.1128/genomeA.01368-17"],["dc.identifier.eissn","2169-8287"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/76806"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Draft Genome Sequence of Vibrio splendidus DSM 19640"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","20221070"],["dc.bibliographiccitation.issue","1984"],["dc.bibliographiccitation.journal","Proceedings of the Royal Society B: Biological Sciences"],["dc.bibliographiccitation.volume","289"],["dc.contributor.author","Wendling, Carolin C."],["dc.contributor.author","Lange, Janina"],["dc.contributor.author","Liesegang, Heiko"],["dc.contributor.author","Sieber, Michael"],["dc.contributor.author","Poehlein, Anja"],["dc.contributor.author","Bunk, Boyke"],["dc.contributor.author","Rajkov, Jelena"],["dc.contributor.author","Goehlich, Henry"],["dc.contributor.author","Roth, Olivia"],["dc.contributor.author","Brockhurst, Michael A."],["dc.date.accessioned","2022-11-01T10:16:57Z"],["dc.date.available","2022-11-01T10:16:57Z"],["dc.date.issued","2022"],["dc.description.abstract","Pathogens vary strikingly in their virulence and the selection they impose on their hosts. While the evolution of different virulence levels is well studied, the evolution of host resistance in response to different virulence levels is less understood and, at present, mainly based on observations and theoretical predictions with few experimental tests. Increased virulence can increase selection for host resistance evolution if the benefits of avoiding infection outweigh resistance costs. To test this, we experimentally evolved the bacterium\n Vibrio alginolyticus\n in the presence of two variants of a filamentous phage that differ in their virulence. The bacterial host exhibited two alternative defence strategies: (1) super infection exclusion (SIE), whereby phage-infected cells were immune to subsequent infection at the cost of reduced growth, and (2) surface receptor mutations (SRM), providing resistance to infection by preventing phage attachment. While SIE emerged rapidly against both phages, SRM evolved faster against the high- than the low-virulence phage. Using a mathematical model of our system, we show that increasing virulence strengthens selection for SRM owing to the higher costs of infection suffered by SIE immune hosts. Thus, by accelerating the evolution of host resistance, more virulent phages caused shorter epidemics."],["dc.description.sponsorship"," Deutsche Forschungsgemeinschaft http://dx.doi.org/10.13039/501100001659"],["dc.identifier.doi","10.1098/rspb.2022.1070"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/116697"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-605"],["dc.relation.eissn","1471-2954"],["dc.relation.issn","0962-8452"],["dc.rights.uri","https://royalsociety.org/journals/ethics-policies/data-sharing-mining/"],["dc.title","Higher phage virulence accelerates the evolution of host resistance"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","7298"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","Applied and Environmental Microbiology"],["dc.bibliographiccitation.lastpage","7309"],["dc.bibliographiccitation.volume","69"],["dc.contributor.author","Schmeisser, C."],["dc.contributor.author","Stockigt, C."],["dc.contributor.author","Raasch, C."],["dc.contributor.author","Wingender, J."],["dc.contributor.author","Timmis, K. N."],["dc.contributor.author","Wenderoth, D. F."],["dc.contributor.author","Flemming, H. C."],["dc.contributor.author","Liesegang, Heiko"],["dc.contributor.author","Schmitz, Ruth A."],["dc.contributor.author","Jaeger, K. E."],["dc.contributor.author","Streit, Wolfgang R."],["dc.date.accessioned","2018-11-07T10:34:13Z"],["dc.date.available","2018-11-07T10:34:13Z"],["dc.date.issued","2003"],["dc.description.abstract","Most naturally occurring biofilms contain a vast majority of microorganisms which have not yet been cultured, and therefore we have little information on the genetic information content of these communities. Therefore, we initiated work to characterize the complex metagenome of model drinking water biofilms grown on rubber-coated valves by employing three different strategies. First, a sequence analysis of 650 16S rRNA clones indicated a high diversity within the biofilm communities, with the majority of the microbes being closely related to the Proteobacteria. Only a small fraction of the 16S rRNA sequences were highly similar to rRNA sequences from Actinobacteria, low-G+C gram-positives and the Cytophaga-Flavobacterium-Bacteroides group. Our second strategy included a snapshot genome sequencing approach. Homology searches in public databases with 5,000 random sequence clones from a small insert library resulted in the identification of 2,200 putative protein-coding sequences, of which 1,026 could be classified into functional groups. Similarity analyses indicated that significant fractions of the genes and proteins identified were highly similar to known proteins observed in the genera Rhizobium, Pseudomonas, and Escherichia. Finally, we report 144 kb of DNA sequence information from four selected cosmid clones, of which two formed a 75-kb overlapping contig. The majority of the proteins identified by whole-cosmid sequencing probably originated from microbes closely related to the alpha-, beta-, and gamma-Proteobacteria. The sequence information was used to set up a database containing the phylogenetic and genomic information on this model microbial community. Concerning the potential health risk of the microbial community studied, no DNA or protein sequences directly linked to pathogenic traits were identified."],["dc.identifier.doi","10.1128/AEM.69.12.7298-7309.2003"],["dc.identifier.isi","000187234000043"],["dc.identifier.pmid","14660379"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/44807"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Soc Microbiology"],["dc.relation.issn","0099-2240"],["dc.title","Metagenome survey of biofilms in drinking-water networks"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Genome Announcements"],["dc.bibliographiccitation.volume","6"],["dc.contributor.author","Hollensteiner, Jacqueline"],["dc.contributor.author","Poehlein, Anja"],["dc.contributor.author","Granzow, Sandra"],["dc.contributor.author","Liesegang, Heiko"],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Vidal, Stefan"],["dc.contributor.author","Wemheuer, Franziska"],["dc.date.accessioned","2020-12-10T18:37:01Z"],["dc.date.available","2020-12-10T18:37:01Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1128/genomeA.01517-17"],["dc.identifier.eissn","2169-8287"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/76814"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Draft Genome Sequence of the Endophyte Bacillus mycoides Strain GM5LP Isolated from Lolium perenne"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1257"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Nature Biotechnology"],["dc.bibliographiccitation.lastpage","1262"],["dc.bibliographiccitation.volume","24"],["dc.contributor.author","Pohlmann, Anne"],["dc.contributor.author","Fricke, Wolfgang Florian"],["dc.contributor.author","Reinecke, Frank"],["dc.contributor.author","Kusian, Bernhard"],["dc.contributor.author","Liesegang, Heiko"],["dc.contributor.author","Cramm, Rainer"],["dc.contributor.author","Eitinger, Thomas"],["dc.contributor.author","Ewering, Christian"],["dc.contributor.author","Poetter, Markus"],["dc.contributor.author","Schwartz, Edward"],["dc.contributor.author","Strittmatter, Axel W."],["dc.contributor.author","Voss, Ingo"],["dc.contributor.author","Gottschalk, Gerhard"],["dc.contributor.author","Steinbuechel, Alexander"],["dc.contributor.author","Friedrich, Baerbel"],["dc.contributor.author","Bowien, Botho"],["dc.date.accessioned","2018-11-07T09:12:07Z"],["dc.date.available","2018-11-07T09:12:07Z"],["dc.date.issued","2006"],["dc.description.abstract","sThe H-2-oxidizing lithoautotrophic bacterium Ralstonia eutropha H16 is a metabolically versatile organism capable of subsisting, in the absence of organic growth substrates, on H-2 and CO2 as its sole sources of energy and carbon. R. eutropha H16 first attracted biotechnological interest nearly 50 years ago with the realization that the organism's ability to produce and store large amounts of poly[R-(-)-3-hydroxybutyrate] and other polyesters could be harnessed to make biodegradable plastics. Here we report the complete genome sequence of the two chromosomes of R. eutropha H16. Together, chromosome 1 (4,052,032 base pairs (bp)) and chromosome 2 (2,912,490 bp) encode 6,116 putative genes. Analysis of the genome sequence offers the genetic basis for exploiting the biotechnological potential of this organism and provides insights into its remarkable metabolic versatility."],["dc.identifier.doi","10.1038/nbt1244"],["dc.identifier.isi","000241191700029"],["dc.identifier.pmid","16964242"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/26879"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Nature Publishing Group"],["dc.relation.issn","1087-0156"],["dc.title","Genome sequence of the bioplastic-producing \"Knallgas\" bacterium Ralstonia eutropha H16"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","365"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Journal of Biotechnology"],["dc.bibliographiccitation.lastpage","369"],["dc.bibliographiccitation.volume","167"],["dc.contributor.author","Rachinger, Michael"],["dc.contributor.author","Bauch, Melanie"],["dc.contributor.author","Strittmatter, Axel W."],["dc.contributor.author","Bongaerts, Johannes"],["dc.contributor.author","Evers, Stefan"],["dc.contributor.author","Maurer, Karl-Heinz"],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Liebl, Wolfgang"],["dc.contributor.author","Liesegang, Heiko"],["dc.contributor.author","Ehrenreich, Armin"],["dc.date.accessioned","2018-11-07T09:19:50Z"],["dc.date.available","2018-11-07T09:19:50Z"],["dc.date.issued","2013"],["dc.description.abstract","Conjugative shuttle vectors of the pKVM series, based on an IncP transfer origin and the pMAD vector with a temperature sensitive replication were constructed to establish a markerless gene deletion protocol for Bacilli without natural competence such as the exoenzyme producer Bacillus licheniformis. The pKVM plasmids can be conjugated to strains of B. licheniformis and B. subtilis. For chromosomal gene deletion, regions flanking the target gene are fused and cloned in a pKVM vector prior to conjugative transfer from Escherichia coli to B. licheniformis. Appropriate markers on the vector backbone allow for the identification of the integration at the target locus and thereafter the vector excision, both events taking place via homologous recombination. The functionality of the deletion system was demonstrated with B. licheniformis by a markerless 939 bp in-frame deletion of the yqfD gene and the deletion of a 31 kbp genomic segment carrying a PBSX-like prophage. (c) 2013 Elsevier B.V. All rights reserved."],["dc.description.sponsorship","BMBF GenoMik-Plus program [0313751A]; GenoMik Design program [0313917D]"],["dc.identifier.doi","10.1016/j.jbiotec.2013.07.026"],["dc.identifier.isi","000324737900002"],["dc.identifier.pmid","23916947"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/28734"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Bv"],["dc.relation.issn","1873-4863"],["dc.relation.issn","0168-1656"],["dc.title","Size unlimited markerless deletions by a transconjugative plasmid-system in Bacillus licheniformis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","547"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Nature Biotechnology"],["dc.bibliographiccitation.lastpage","553"],["dc.bibliographiccitation.volume","22"],["dc.contributor.author","Henne, A."],["dc.contributor.author","Bruggemann, H."],["dc.contributor.author","Raasch, C."],["dc.contributor.author","Wiezer, A."],["dc.contributor.author","Hartsch, T."],["dc.contributor.author","Liesegang, Heiko"],["dc.contributor.author","Johann, A."],["dc.contributor.author","Lienard, T."],["dc.contributor.author","Gohl, O."],["dc.contributor.author","Martinez-Arias, R."],["dc.contributor.author","Jacobi, C."],["dc.contributor.author","Starkuviene, V."],["dc.contributor.author","Schlenczeck, S."],["dc.contributor.author","Dencker, S."],["dc.contributor.author","Huber, R."],["dc.contributor.author","Klenk, H. P."],["dc.contributor.author","Kramer, W."],["dc.contributor.author","Merkl, R."],["dc.contributor.author","Gottschalk, G."],["dc.contributor.author","Fritz, Hans-Joachim"],["dc.date.accessioned","2018-11-07T10:49:25Z"],["dc.date.available","2018-11-07T10:49:25Z"],["dc.date.issued","2004"],["dc.description.abstract","Thermus thermophilus HB27 is an extremely thermophilic, halotolerant bacterium, which was originally isolated from a natural thermal environment in Japan. This organism has considerable biotechnological potential; many thermostable proteins isolated from members of the genus Thermus are indispensable in research and in industrial applications. We present here the complete genome sequence of T. thermophilus HB27, the first for the genus Thermus. The genome consists of a 1,894,877 base pair chromosome and a 232,605 base pair megaplasmid, designated pTT27. The 2,218 identified putative genes were compared to those of the closest relative sequenced so far, the mesophilic bacterium Deinococcus radiodurans. Both organisms share a similar set of proteins, although their genomes lack extensive synteny. Many new genes of potential interest for biotechnological applications were found in T. thermophilus HB27. Candidates include various proteases and key enzymes of other fundamental biological processes such as DNA replication, DNA repair and RNA maturation."],["dc.identifier.doi","10.1038/nbt956"],["dc.identifier.isi","000221159700022"],["dc.identifier.pmid","15064768"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/48423"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Nature Publishing Group"],["dc.relation.issn","1087-0156"],["dc.title","The genome sequence of the extreme thermophile Thermus thermophilus"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]