Now showing 1 - 5 of 5
  • 2014Journal Article Research Paper
    [["dc.bibliographiccitation.artnumber","088102"],["dc.bibliographiccitation.firstpage","3"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Physical Review Letters"],["dc.bibliographiccitation.lastpage","7"],["dc.bibliographiccitation.volume","112"],["dc.contributor.author","Weinhausen, Britta"],["dc.contributor.author","Saldanha, Oliva"],["dc.contributor.author","Wilke, Robin N."],["dc.contributor.author","Dammann, Christian"],["dc.contributor.author","Priebe, Marius"],["dc.contributor.author","Burghammer, Manfred"],["dc.contributor.author","Sprung, Michael"],["dc.contributor.author","Köster, Sarah"],["dc.date.accessioned","2017-09-07T11:46:29Z"],["dc.date.available","2017-09-07T11:46:29Z"],["dc.date.issued","2014"],["dc.description.abstract","High-resolution x-ray imaging techniques offer a variety of possibilities for studying the nanoscale structure of biological cells. A challenging task remains the study of cells by x rays in their natural, aqueous environment. Here, we overcome this limitation by presenting scanning x-ray diffraction measurements with beam sizes in the range of a few hundred nm on living and fixed-hydrated eukaryotic cells in microfluidic devices which mimic a native environment. The direct comparison between fixed-hydrated and living cells shows distinct differences in the scattering signal, pointing to structural changes on the order of 30 to 50 nm."],["dc.identifier.doi","10.1103/PhysRevLett.112.088102"],["dc.identifier.gro","3142182"],["dc.identifier.isi","000331957600012"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/5443"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1079-7114"],["dc.relation.issn","0031-9007"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Köster (Cellular Biophysics)"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","x-ray scattering"],["dc.subject.gro","cellular biophysics"],["dc.subject.gro","microfluidics"],["dc.title","Scanning X-Ray Nanodiffraction on Living Eukaryotic Cells in Microfluidic Environments"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]
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  • 2015Journal Article Research Paper
    [["dc.bibliographiccitation.firstpage","380"],["dc.bibliographiccitation.issue","3-4"],["dc.bibliographiccitation.journal","Geomicrobiology Journal"],["dc.bibliographiccitation.lastpage","393"],["dc.bibliographiccitation.volume","32"],["dc.contributor.author","Wilke, Robin N."],["dc.contributor.author","Quéric, Nadia Valérie"],["dc.contributor.author","Hoppert, Michael"],["dc.contributor.author","Heller, C."],["dc.contributor.author","Schropp, A."],["dc.contributor.author","Schroer, C. G."],["dc.contributor.author","Burghammer, Manfred"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","Reitner, Joachim"],["dc.date.accessioned","2017-09-07T11:44:30Z"],["dc.date.available","2017-09-07T11:44:30Z"],["dc.date.issued","2015"],["dc.description.abstract","Modern scanning X-ray microscopy can help to unravel the spatial context between biotic and abiotic compounds of geobiological assemblies with the aim to finally link chemical pathways to biological activities at the nanometre scale. This work presents some multi-modal imaging techniques provided by hard X-ray microscopes at synchrotron radiation sources to address analytical needs in geobiological research. Using the examples of 1\\) a calcified basal skeleton of the demosponge Astrosclera willeyana, 2\\) an anaerobic methane-oxidizing microbial mat and 3\\) a bacterial sulfur-oxidizing consortium, we illustrate the potential of scanning X-ray fluorescence and scanning transmission X-ray microscopy, and a novel quantitative approach of ptychographic imaging at single cell level."],["dc.identifier.doi","10.1080/01490451.2014.908982"],["dc.identifier.gro","3141938"],["dc.identifier.isi","000352349600016"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2746"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1521-0529"],["dc.relation.issn","0149-0451"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","x-ray scattering"],["dc.title","Scanning Hard X-ray Microscopy Imaging Modalities for Geobiological Samples"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]
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  • 2015Journal Article Research Paper
    [["dc.bibliographiccitation.firstpage","1818"],["dc.bibliographiccitation.journal","Journal of Applied Crystallography"],["dc.bibliographiccitation.lastpage","1826"],["dc.bibliographiccitation.volume","48"],["dc.contributor.author","Wallentin, Jesper"],["dc.contributor.author","Wilke, Robin N."],["dc.contributor.author","Osterhoff, Markus"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2017-09-07T11:54:51Z"],["dc.date.available","2017-09-07T11:54:51Z"],["dc.date.issued","2015"],["dc.description.abstract","Simultaneous scanning Bragg contrast and small-angle ptychographic imaging of a single solar cell nanowire are demonstrated, using a nanofocused hard X-ray beam and two detectors. The 2.5 mu m-long nanowire consists of a single-crystal InP core of 190 nm diameter, coated with amorphous SiO2 and polycrystalline indium tin oxide. The nanowire was selected and aligned in real space using the small-angle scattering of the 140 x 210 nm X-ray beam. The orientation of the nanowire, as observed in small-angle scattering, was used to find the correct rotation for the Bragg condition. After alignment in real space and rotation, high-resolution (50 nm step) raster scans were performed to simultaneously measure the distribution of small-angle scattering and Bragg diffraction in the nanowire. Ptychographic reconstruction of the coherent small-angle scattering was used to achieve sub-beam spatial resolution. The small-angle scattering images, which are sensitive to the shape and the electron density of all parts of the nanowire, showed a homogeneous profile along the nanowire axis except at the thicker head region. In contrast, the scanning Bragg diffraction microscopy, which probes only the single-crystal InP core, revealed bending and crystalline inhomogeneity. Both systematic and non-systematic real-space movement of the nanowire were observed as it was rotated, which would have been difficult to reveal only from the Bragg scattering. These results demonstrate the advantages of simultaneously collecting and analyzing the small-angle scattering in Bragg diffraction experiments."],["dc.identifier.doi","10.1107/S1600576715017975"],["dc.identifier.gro","3141777"],["dc.identifier.isi","000365774800026"],["dc.identifier.pmid","26664342"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12753"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/957"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: K.A. Wallenberg Foundation; Deutsche Forschungsgemeinschaft [SFB 755]"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1600-5767"],["dc.relation.orgunit","Fakultät für Physik"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","x-ray scattering"],["dc.title","Simultaneous high-resolution scanning Bragg contrast and ptychographic imaging of a single solar cell nanowire"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]
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  • 2015Journal Article Research Paper
    [["dc.bibliographiccitation.firstpage","867"],["dc.bibliographiccitation.journal","Journal of Synchrotron Radiation"],["dc.bibliographiccitation.lastpage","878"],["dc.bibliographiccitation.volume","22"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","Osterhoff, Markus"],["dc.contributor.author","Krenkel, Martin"],["dc.contributor.author","Wilke, Robin N."],["dc.contributor.author","Priebe, Marius"],["dc.contributor.author","Bartels, Matthias"],["dc.contributor.author","Kalbfleisch, Sebastian"],["dc.contributor.author","Sprung, Michael"],["dc.date.accessioned","2017-09-07T11:43:44Z"],["dc.date.available","2017-09-07T11:43:44Z"],["dc.date.issued","2015"],["dc.description.abstract","A compound optical system for coherent focusing and imaging at the nanoscale is reported, realised by high-gain fixed-curvature elliptical mirrors in combination with X-ray waveguide optics or different cleaning apertures. The key optical concepts are illustrated, as implemented at the Gottingen Instrument for Nano-Imaging with X-rays (GINIX), installed at the P10 coherence beamline of the PETRA III storage ring at DESY, Hamburg, and examples for typical applications in biological imaging are given. Characteristic beam configurations with the recently achieved values are also described, meeting the different requirements of the applications, such as spot size, coherence or bandwidth. The emphasis of this work is on the different beam shaping, filtering and characterization methods."],["dc.identifier.doi","10.1107/S1600577515007742"],["dc.identifier.gro","3141875"],["dc.identifier.isi","000357407900001"],["dc.identifier.pmid","26134789"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2045"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation","SFB 755: Nanoscale Photonic Imaging"],["dc.relation.eissn","1600-5775"],["dc.relation.issn","0909-0495"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray optics"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","x-ray scattering"],["dc.title","Compound focusing mirror and X-ray waveguide optics for coherent imaging and nano-diffraction"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]
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  • 2012Journal Article Research Paper
    [["dc.bibliographiccitation.firstpage","19232"],["dc.bibliographiccitation.issue","17"],["dc.bibliographiccitation.journal","Optics Express"],["dc.bibliographiccitation.lastpage","19254"],["dc.bibliographiccitation.volume","20"],["dc.contributor.author","Wilke, Robin N."],["dc.contributor.author","Priebe, Marius"],["dc.contributor.author","Bartels, Matthias"],["dc.contributor.author","Giewekemeyer, Klaus"],["dc.contributor.author","Diaz, Ana"],["dc.contributor.author","Karvinen, P."],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2017-09-07T11:48:27Z"],["dc.date.available","2017-09-07T11:48:27Z"],["dc.date.issued","2012"],["dc.description.abstract","Ptychographic coherent X-ray diffractive imaging (PCDI) has been combined with nano-focus X-ray diffraction to study the structure and density distribution of unstained and unsliced bacterial cells, using a hard X-ray beam of 6.2keV photon energy, focused to about 90nm by a Fresnel zone plate lens. While PCDI provides images of the bacteria with quantitative contrast in real space with a resolution well below the beam size at the sample, spatially resolved small angle X-ray scattering using the same Fresnel zone plate (cellular nano-diffraction) provides structural information at highest resolution in reciprocal space up to 2nm(-1). We show how the real and reciprocal space approach can be used synergistically on the same sample and with the same setup. In addition, we present 3D hard X-ray imaging of unstained bacterial cells by a combination of ptychography and tomography. (C) 2012 Optical Society of America"],["dc.identifier.doi","10.1364/OE.20.019232"],["dc.identifier.fs","589591"],["dc.identifier.gro","3142482"],["dc.identifier.isi","000307873600075"],["dc.identifier.pmid","23038565"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9566"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/8773"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: Deutsche Forschungsgemeinschaft [SFB 755]"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1094-4087"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.orgunit","Fakultät für Physik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","x-ray scattering"],["dc.title","Hard X-ray imaging of bacterial cells: nano-diffraction and ptychographic reconstruction"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]
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