Dev, Arvind A.

[["dc.bibliographiccitation.firstpage","155"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","MHR Basic science of reproductive medicine"],["dc.bibliographiccitation.lastpage","163"],["dc.bibliographiccitation.volume","13"],["dc.contributor.author","Jaroszynski, L."],["dc.contributor.author","Dev, A."],["dc.contributor.author","Li, M."],["dc.contributor.author","Meinhardt, Andreas"],["dc.contributor.author","de Rooij, D. G."],["dc.contributor.author","Mueller, Christian"],["dc.contributor.author","Boehm, Detlef"],["dc.contributor.author","Wolf, S."],["dc.contributor.author","Adham, Ibrahim M."],["dc.contributor.author","Wulf, Gerald"],["dc.contributor.author","Engel, Wolfgang"],["dc.contributor.author","Nayernia, K."],["dc.date.accessioned","2018-11-07T11:04:40Z"],["dc.date.available","2018-11-07T11:04:40Z"],["dc.date.issued","2007"],["dc.description.abstract","Testis expressed gene 18 (Tex18) is a small gene with one exon of 240 bp, which is specifically expressed in male germ cells. The gene encodes for a protein of 80 amino acids with unknown domain. To investigate the function of (Tex18) gene, we generated mice with targeted disruption of the (Tex18) gene by homologous recombination. Homozygous mutant males on a mixed genetic background (C57BL/6J x 129/Sv) are fertile, while they are subfertile on the 129/Sv background, although mating is normal. We showed that Tex18(-/-) males are subfertile because of abnormal sperm morphology and reduced motility, which is called asthenoteratozoospermia, suggesting that (Tex18) affects sperm characteristics. Maturation of spermatids is unsynchronized and partially impaired in seminiferous tubules of Tex18(-/-) mice. Electron microscopical examination demonstrated abnormal structures of sperm head. In vivo experiments with sperm of Tex18(-/-) 129/Sv mice revealed that the migration of spermatozoa from the uterus into the oviduct is reduced. This result is supported by the observation that sperm motility, as determined by the computer-assisted semen analysis system, is significantly affected, compared to wild-type spermatozoa. Generation of transgenic mice containing Tex18-EGFP fusion construct revealed a high transcriptional activity of (Tex18) during spermiogenesis, a process with morphological changes of haploid germ cells and development to mature spermatozoa. These results indicate that (Tex18) is expressed predominantly during spermatid differentiation and subfertility of the male Tex18(-/-) mice on the 129/Sv background is due to the differentiation arrest, abnormal sperm morphology and reduced sperm motility."],["dc.identifier.doi","10.1093/molehr/gal107"],["dc.identifier.isi","000244662300003"],["dc.identifier.pmid","17208930"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/51894"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press"],["dc.relation.issn","1360-9947"],["dc.title","Asthenoteratozoospermia in mice lacking testis expressed gene 18 (Tex18)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1456"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Molecular Reproduction and Development"],["dc.bibliographiccitation.lastpage","1464"],["dc.bibliographiccitation.volume","74"],["dc.contributor.author","Dev, Arvind"],["dc.contributor.author","Nayernia, Karim"],["dc.contributor.author","Meins, Moritz"],["dc.contributor.author","Adham, Ibrahim M."],["dc.contributor.author","Lacone, Franco"],["dc.contributor.author","Engel, Wolfgang"],["dc.date.accessioned","2018-11-07T10:57:16Z"],["dc.date.available","2018-11-07T10:57:16Z"],["dc.date.issued","2007"],["dc.description.abstract","RNA-binding proteins are involved in post-transcriptional processes like mRNA stabilization, alternative splicing, and transport. Brunol1 is a novel mouse gene related to elav/Bruno family of genes encoding for RNA-binding proteins. We report here the expression and functional analysis of murine Brunol1. Expression analysis of Brunol1 during embryogenesis by RT-PCR showed that Brunol1 expression starts at 9.5 dpc and continues to the later stages of embryonic development. In adult mice, the Brunol1 expression is restricted to brain and testis. We also analyzed the Brunol1 expression in testes of different mutants with spermatogenesis defects: W/W-V, Tfm/y, Leyl(-/-), olt/olt, and qk/qk. Brunol1 transcript was detectable in Leyl-/-, olt/olt, and qk/qk mutant but not in W/W-V and Tfm/y mutants. We also showed by transfection of a fusion protein of green fluorescent protein and Brunol1 protein into NIH3T3 cells, that Brunol1 is localized in cytoplasm and nucleus. In order to elucidate the function of the Brunol1 protein in spermatogenesis, we disrupted the Brunol1 locus in mouse by homologous recombination, which resulted in a complete loss of the Brunol1 transcript. Male and female Brunol1(-/-) and Brunol1(-/-) mice from genetic backgrounds C57BU6J x 129/Sv hybrid and 129X1/SvJ when inbred exhibited normal phenotype and are fertile, although the number and motility of sperms are significantly reduced. An intensive phenotypic analysis showed no gross abnormalities in testis morphology. Collectively our results demonstrate that Brunol1 might be nonessential protein for mouse embryonic development and sperm atogenesis."],["dc.identifier.doi","10.1002/mrd.20742"],["dc.identifier.isi","000249677800012"],["dc.identifier.pmid","17393433"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/50201"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-liss"],["dc.relation.issn","1040-452X"],["dc.title","Mice deficient for RNA-Binding protein brunoll show reduction of sperniatogenesis but are fertile"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","273"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Molecular Reproduction and Development"],["dc.bibliographiccitation.lastpage","279"],["dc.bibliographiccitation.volume","74"],["dc.contributor.author","Tseden, Khailun"],["dc.contributor.author","Topaloglu, Ozlem"],["dc.contributor.author","Meinhardt, Andreas"],["dc.contributor.author","Dev, Arvind"],["dc.contributor.author","Adham, Ibrahim M."],["dc.contributor.author","Mueller, Christian"],["dc.contributor.author","Wolf, Stephan"],["dc.contributor.author","Boehm, Detlef"],["dc.contributor.author","Schlueter, Gregor"],["dc.contributor.author","Engel, Wolfgang"],["dc.contributor.author","Nayernia, Karim"],["dc.date.accessioned","2018-11-07T11:04:41Z"],["dc.date.available","2018-11-07T11:04:41Z"],["dc.date.issued","2007"],["dc.description.abstract","During mammalian spermiogenesis somatic histones are replaced at first by transition proteins, which are in turn replaced by the protamines, forming the sperm nucleoprotamines. It is believed that transition protein 2 (Tnp2) is necessary for maintaining the normal processing of protamines and, consequently, the completion of chromatin condensation. The transition protein mRNAs are stored in translationally inert messenger ribonucleoprotein particles for up to 7 days until translational activation in elongated spermatids. Substantial evidence suggests an involvement of 3'untranslated region (UTR) in the translational regulation of the Tnp2 mRNAs. In order to determine the role of Tnp2 3'UTR in translational regulation and to study whether the translational repression of Tnp2 mRNA is necessary for normal spermatid differentiation in mice, we generated transgenic mice that carry a Tnp2-hGH transgene. In this transgene, 3'UTR of Thp2 gene was replaced by 3' 3'UTR of human growth hormone gene. In these transgenic animals, transcription and translation of Tnp2 occur simultaneously in round spermatids which is an evidence for involvement of Tnp2 3'UTR in its translation repression. Premature translation of Tnp2 mRNA caused abnormal head morphogenesis, reduced sperm motility and male infertility. These results show clearly that a strict temporal and stage-specific Tnp2 translation is necessary for the correct differentiation of round spermatids into mature spermatozoa and for male fertility."],["dc.identifier.doi","10.1002/mrd.20570"],["dc.identifier.isi","000243567900002"],["dc.identifier.pmid","16967499"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/51896"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-liss"],["dc.relation.issn","1040-452X"],["dc.title","Premature translation of transition protein 2 mRNA causes sperm abnormalities and male infertility"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]