Dev, Arvind A.

[["dc.bibliographiccitation.firstpage","125"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Developmental Cell"],["dc.bibliographiccitation.lastpage","132"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Nayernia, Karim"],["dc.contributor.author","Nolte, Jessica"],["dc.contributor.author","Michelmann, Hans Wilhelm"],["dc.contributor.author","Lee, Jae Ho"],["dc.contributor.author","Rathsack, Kristina"],["dc.contributor.author","Drusenheimer, Nadja"],["dc.contributor.author","Dev, Arvind"],["dc.contributor.author","Wulf, Gerald"],["dc.contributor.author","Ehrmann, Ingrid E."],["dc.contributor.author","Elliott, David J."],["dc.contributor.author","Okpanyi, Vera"],["dc.contributor.author","Zechner, Ulrich"],["dc.contributor.author","Haaf, Thomas"],["dc.contributor.author","Meinhardt, Andreas"],["dc.contributor.author","Engel, Wolfgang"],["dc.date.accessioned","2018-11-07T09:35:07Z"],["dc.date.available","2018-11-07T09:35:07Z"],["dc.date.issued","2006"],["dc.description.abstract","Male gametes originate from a small population of spermatogonial stem cells (SSCs). These cells are believed to divide infinitely and to support spermatogenesis throughout life in the male. Here, we developed a strategy for the establishment of SSC lines from embryonic stem (ES) cells. These cells are able to undergo meiosis, are able to generate haploid male gametes in vitro, and are functional, as shown by fertilization after intracytoplasmic injection into mouse oocytes. Resulting two-cell embryos were transferred into oviducts, and live mice were born. Six of seven animals developed to adult mice. This is a clear indication that male gametes derived in vitro from ES cells by this strategy are able to induce normal fertilization and development. Our approach provides an accessible in vitro model system for studies of mammalian gametogenesis, as well as for the development of new strategies for the generation of transgenic mice and treatment of infertility."],["dc.identifier.doi","10.1016/j.devcel.2006.05.010"],["dc.identifier.isi","000239128300015"],["dc.identifier.pmid","16824959"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/32324"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cell Press"],["dc.relation.issn","1534-5807"],["dc.title","In vitro-differentiated embryonic stem cells give rise to male gametes that can generate offspring mice"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","432"],["dc.bibliographiccitation.issue","29-30"],["dc.bibliographiccitation.journal","Swiss Medical Weekly"],["dc.bibliographiccitation.lastpage","436"],["dc.bibliographiccitation.volume","138"],["dc.contributor.author","Argyriou, Loukas"],["dc.contributor.author","Wirbelauer, Johannes"],["dc.contributor.author","Dev, Arvind"],["dc.contributor.author","Panchulidze, Irakli"],["dc.contributor.author","Shoukier, Moneef"],["dc.contributor.author","Teske, Ute"],["dc.contributor.author","Nayernia, Karim"],["dc.date.accessioned","2018-11-07T11:12:53Z"],["dc.date.available","2018-11-07T11:12:53Z"],["dc.date.issued","2008"],["dc.description.abstract","Hereditary haemorrhagic telangiectasia (HHT), associated with arteriovenous malformations, is a genetic disease of the vascular system with a frequency of approx. 1:10,000. Genetic diagnosis serves to identify individuals at risk of developing the disease and is a useful tool for genetic counselling purposes. Questions under study: Here we report on a child presenting severe arteriovenous malformations leading to heart failure. Her mother and grandmother present fewer symptoms of hereditary haemorrhagic telangiectasia. In this study we identify the cause of HHT in the family. Methods: Clinical examination, PCR, DNA sequencing, quantitative PCR, Southern blot, x-ray, ultrasound, cardiac catheterisation and angiocardiography. Results: Initially the sequence variant in c.392C > T in the endoglin gene was detected in the grandmother, but not in other affected family members. Further analyses revealed a deletion of exon 1 of endoglin, segregating with the phenotype. Conclusions: This report points out the need for careful evaluation of molecular genetic findings, particularly in diseases with highly variable phenotype."],["dc.identifier.isi","000257973200004"],["dc.identifier.pmid","18654869"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/53767"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","E M H Swiss Medical Publishers Ltd"],["dc.relation.issn","1424-7860"],["dc.title","A newborn with hereditary haemorrhagic telangiectasia and an unusually severe phenotype"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1456"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Molecular Reproduction and Development"],["dc.bibliographiccitation.lastpage","1464"],["dc.bibliographiccitation.volume","74"],["dc.contributor.author","Dev, Arvind"],["dc.contributor.author","Nayernia, Karim"],["dc.contributor.author","Meins, Moritz"],["dc.contributor.author","Adham, Ibrahim M."],["dc.contributor.author","Lacone, Franco"],["dc.contributor.author","Engel, Wolfgang"],["dc.date.accessioned","2018-11-07T10:57:16Z"],["dc.date.available","2018-11-07T10:57:16Z"],["dc.date.issued","2007"],["dc.description.abstract","RNA-binding proteins are involved in post-transcriptional processes like mRNA stabilization, alternative splicing, and transport. Brunol1 is a novel mouse gene related to elav/Bruno family of genes encoding for RNA-binding proteins. We report here the expression and functional analysis of murine Brunol1. Expression analysis of Brunol1 during embryogenesis by RT-PCR showed that Brunol1 expression starts at 9.5 dpc and continues to the later stages of embryonic development. In adult mice, the Brunol1 expression is restricted to brain and testis. We also analyzed the Brunol1 expression in testes of different mutants with spermatogenesis defects: W/W-V, Tfm/y, Leyl(-/-), olt/olt, and qk/qk. Brunol1 transcript was detectable in Leyl-/-, olt/olt, and qk/qk mutant but not in W/W-V and Tfm/y mutants. We also showed by transfection of a fusion protein of green fluorescent protein and Brunol1 protein into NIH3T3 cells, that Brunol1 is localized in cytoplasm and nucleus. In order to elucidate the function of the Brunol1 protein in spermatogenesis, we disrupted the Brunol1 locus in mouse by homologous recombination, which resulted in a complete loss of the Brunol1 transcript. Male and female Brunol1(-/-) and Brunol1(-/-) mice from genetic backgrounds C57BU6J x 129/Sv hybrid and 129X1/SvJ when inbred exhibited normal phenotype and are fertile, although the number and motility of sperms are significantly reduced. An intensive phenotypic analysis showed no gross abnormalities in testis morphology. Collectively our results demonstrate that Brunol1 might be nonessential protein for mouse embryonic development and sperm atogenesis."],["dc.identifier.doi","10.1002/mrd.20742"],["dc.identifier.isi","000249677800012"],["dc.identifier.pmid","17393433"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/50201"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-liss"],["dc.relation.issn","1040-452X"],["dc.title","Mice deficient for RNA-Binding protein brunoll show reduction of sperniatogenesis but are fertile"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","273"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Molecular Reproduction and Development"],["dc.bibliographiccitation.lastpage","279"],["dc.bibliographiccitation.volume","74"],["dc.contributor.author","Tseden, Khailun"],["dc.contributor.author","Topaloglu, Ozlem"],["dc.contributor.author","Meinhardt, Andreas"],["dc.contributor.author","Dev, Arvind"],["dc.contributor.author","Adham, Ibrahim M."],["dc.contributor.author","Mueller, Christian"],["dc.contributor.author","Wolf, Stephan"],["dc.contributor.author","Boehm, Detlef"],["dc.contributor.author","Schlueter, Gregor"],["dc.contributor.author","Engel, Wolfgang"],["dc.contributor.author","Nayernia, Karim"],["dc.date.accessioned","2018-11-07T11:04:41Z"],["dc.date.available","2018-11-07T11:04:41Z"],["dc.date.issued","2007"],["dc.description.abstract","During mammalian spermiogenesis somatic histones are replaced at first by transition proteins, which are in turn replaced by the protamines, forming the sperm nucleoprotamines. It is believed that transition protein 2 (Tnp2) is necessary for maintaining the normal processing of protamines and, consequently, the completion of chromatin condensation. The transition protein mRNAs are stored in translationally inert messenger ribonucleoprotein particles for up to 7 days until translational activation in elongated spermatids. Substantial evidence suggests an involvement of 3'untranslated region (UTR) in the translational regulation of the Tnp2 mRNAs. In order to determine the role of Tnp2 3'UTR in translational regulation and to study whether the translational repression of Tnp2 mRNA is necessary for normal spermatid differentiation in mice, we generated transgenic mice that carry a Tnp2-hGH transgene. In this transgene, 3'UTR of Thp2 gene was replaced by 3' 3'UTR of human growth hormone gene. In these transgenic animals, transcription and translation of Tnp2 occur simultaneously in round spermatids which is an evidence for involvement of Tnp2 3'UTR in its translation repression. Premature translation of Tnp2 mRNA caused abnormal head morphogenesis, reduced sperm motility and male infertility. These results show clearly that a strict temporal and stage-specific Tnp2 translation is necessary for the correct differentiation of round spermatids into mature spermatozoa and for male fertility."],["dc.identifier.doi","10.1002/mrd.20570"],["dc.identifier.isi","000243567900002"],["dc.identifier.pmid","16967499"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/51896"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-liss"],["dc.relation.issn","1040-452X"],["dc.title","Premature translation of transition protein 2 mRNA causes sperm abnormalities and male infertility"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","41"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE"],["dc.bibliographiccitation.lastpage","44"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Nayernia, Karim"],["dc.contributor.author","Lee, Jae Ho"],["dc.contributor.author","Engel, Wolfgang"],["dc.contributor.author","Nolte, Jessica"],["dc.contributor.author","Drusenheimer, Nadja"],["dc.contributor.author","Rathsack, Kristina"],["dc.contributor.author","Dev, Arvind"],["dc.contributor.author","Wulf, Gerald"],["dc.contributor.author","Ehrmann, Ingrid E."],["dc.contributor.author","Elliott, David J."],["dc.contributor.author","Zechner, Ulrich"],["dc.contributor.author","Haaf, Thomas"],["dc.contributor.author","Meinhardt, Andreas"],["dc.contributor.author","Michelmann, Hans Wilhelm"],["dc.contributor.author","Hasenfuss, Gerlad"],["dc.contributor.author","Guan, Kaomei"],["dc.date.accessioned","2018-11-07T11:04:31Z"],["dc.date.available","2018-11-07T11:04:31Z"],["dc.date.issued","2007"],["dc.description.abstract","Germline and somatic stem cells are distinct types of stem cells that are dedicated to reproduction and somatic tissue regeneration, respectively. Germline stem cells (GSCs), which can self-renew and generate gametes, are unique stem cells in that they are solely dedicated to transmit genetic information from generation to generation. We developed a strategy for the establishment of germline stem cell lines from embryonic stem cells (ES). These cells are able to undergo meiosis, generate haploid male gametes in vitro and are functional, as shown by fertilization after intra-cytoplasmic injection into mouse oocytes. In other approach, we show that bone marrow stem (BMS) cells are able to trans-differentiate into male germ cells. BMS cell-derived germ cells expressed the known molecular markers of primordial germ cells. The ability to derive male germ cells from ES and BMS cells reveals novel aspects of germ cell development and opens the possibilities for use of these cells in reproductive medicine. Conversely, we showed that adult male germline stem cells, spermatogonial stem cells (SSCs), can be converted into embryonic stem cell like cells which can differentiate into the somatic stem cells of three germ layers. Understanding how SSC can give rise to pluripotent stem cells and how somatic stem cells differentiate into germ cells could give significant insights into the regulation of developmental totipotency as well as having important implications for male fertility and regenerative medicine."],["dc.identifier.isi","000254385400001"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/51863"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.issn","1680-6433"],["dc.title","From stem cells to germ cells and from germ cells to stem cells"],["dc.type","review"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]