Pöhlmann, Stefan

[["dc.bibliographiccitation.firstpage","5210"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Nucleic Acids Research"],["dc.bibliographiccitation.lastpage","5222"],["dc.bibliographiccitation.volume","47"],["dc.contributor.author","Korniy, Natalia"],["dc.contributor.author","Goyal, Akanksha"],["dc.contributor.author","Hoffmann, Markus"],["dc.contributor.author","Samatova, Ekaterina"],["dc.contributor.author","Peske, Frank"],["dc.contributor.author","Pöhlmann, Stefan"],["dc.contributor.author","Rodnina, Marina V"],["dc.date.accessioned","2020-12-10T18:19:35Z"],["dc.date.available","2020-12-10T18:19:35Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1093/nar/gkz202"],["dc.identifier.eissn","1362-4962"],["dc.identifier.issn","0305-1048"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16451"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/75304"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.intern","Merged from goescholar"],["dc.rights","CC BY-NC 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc/4.0"],["dc.title","Modulation of HIV-1 Gag/Gag-Pol frameshifting by tRNA abundance"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e97695"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","PLOS ONE"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Eckert, Nadine"],["dc.contributor.author","Wrensch, Florian"],["dc.contributor.author","Gärtner, Sabine"],["dc.contributor.author","Palanisamy, Navaneethan"],["dc.contributor.author","Goedecke, Ulrike"],["dc.contributor.author","Jäger, Nils"],["dc.contributor.author","Pöhlmann, Stefan"],["dc.contributor.author","Winkler, Michael"],["dc.date.accessioned","2019-07-09T11:39:43Z"],["dc.date.available","2019-07-09T11:39:43Z"],["dc.date.issued","2014"],["dc.description.abstract","Reporter genes inserted into viral genomes enable the easy and rapid quantification of virus replication, which is instrumental to efficient in vitro screening of antiviral compounds or in vivo analysis of viral spread and pathogenesis. Based on a published design, we have generated several replication competent influenza A viruses carrying either fluorescent proteins or Gaussia luciferase. Reporter activity could be readily quantified in infected cultures, but the virus encoding Gaussia luciferase was more stable than viruses bearing fluorescent proteins and was therefore analyzed in detail. Quantification of Gaussia luciferase activity in the supernatants of infected culture allowed the convenient and highly sensitive detection of viral spread, and enzymatic activity correlated with the number of infectious particles released from infected cells. Furthermore, the Gaussia luciferase encoding virus allowed the sensitive quantification of the antiviral activity of the neuraminidase inhibitor (NAI) zanamivir and the host cell interferon-inducible transmembrane (IFITM) proteins 1–3, which are known to inhibit influenza virus entry. Finally, the virus was used to demonstrate that influenza A virus infection is sensitive to a modulator of endosomal cholesterol, in keeping with the concept that IFITMs inhibit viral entry by altering cholesterol levels in the endosomal membrane. In sum, we report the characterization of a novel influenza A reporter virus, which allows fast and sensitive detection of viral spread and its inhibition, and we show that influenza A virus entry is sensitive to alterations of endosomal cholesterol levels."],["dc.identifier.doi","10.1371/journal.pone.0097695"],["dc.identifier.pmid","24842154"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/10118"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/58030"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Influenza A Virus Encoding Secreted Gaussia Luciferase as Useful Tool to Analyze Viral Replication and Its Inhibition by Antiviral Compounds and Cellular Proteins"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e0284"],["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Critical Care Explorations"],["dc.bibliographiccitation.lastpage","5"],["dc.bibliographiccitation.volume","2"],["dc.contributor.author","Hofmann-Winkler, Heike"],["dc.contributor.author","Moerer, Onnen"],["dc.contributor.author","Alt-Epping, Sabine"],["dc.contributor.author","Bräuer, Anselm"],["dc.contributor.author","Büttner, Benedikt"],["dc.contributor.author","Müller, Martin"],["dc.contributor.author","Fricke, Torben"],["dc.contributor.author","Grundmann, Julian"],["dc.contributor.author","Harnisch, Lars-Olav"],["dc.contributor.author","Heise, Daniel"],["dc.contributor.author","Kernchen, Andrea"],["dc.contributor.author","Pressler, Meike"],["dc.contributor.author","Stephani, Caspar"],["dc.contributor.author","Tampe, Björn"],["dc.contributor.author","Kaul, Artur"],["dc.contributor.author","Gärtner, Sabine"],["dc.contributor.author","Kramer, Stefanie"],["dc.contributor.author","Pöhlmann, Stefan"],["dc.contributor.author","Winkler, Martin Sebastian"],["dc.date.accessioned","2020-11-27T11:23:20Z"],["dc.date.accessioned","2021-10-27T13:22:21Z"],["dc.date.available","2020-11-27T11:23:20Z"],["dc.date.available","2021-10-27T13:22:21Z"],["dc.date.issued","2020"],["dc.description.abstract","Objectives: Severe acute respiratory syndrome coronavirus 2 cell entry depends on angiotensin-converting enzyme 2 and transmembrane serine protease 2 and is blocked in cell culture by camostat mesylate, a clinically proven protease inhibitor. Whether camostat mesylate is able to lower disease burden in coronavirus disease 2019 sepsis is currently unknown. Design: Retrospective observational case series. Setting: Patient treated in ICU of University hospital Göttingen, Germany. Patients: Eleven critical ill coronavirus disease 2019 patients with organ failure were treated in ICU. Interventions: Compassionate use of camostat mesylate (six patients, camostat group) or hydroxychloroquine (five patients, hydroxychloroquine group). Measurements and Main Results: Clinical courses were assessed by Sepsis-related Organ Failure Assessment score at days 1, 3, and 8. Further, viral load, oxygenation, and inflammatory markers were determined. Sepsis-related Organ Failure Assessment score was comparable between camostat and hydroxychloroquine groups upon ICU admission. During observation, the Sepsis-related Organ Failure Assessment score decreased in the camostat group but remained elevated in the hydroxychloroquine group. The decline in disease severity in camostat mesylate treated patients was paralleled by a decline in inflammatory markers and improvement of oxygenation. Conclusions: The severity of coronavirus disease 2019 decreased upon camostat mesylate treatment within a period of 8 days and a similar effect was not observed in patients receiving hydroxychloroquine. Camostat mesylate thus warrants further evaluation within randomized clinical trials."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2020"],["dc.identifier.doi","10.1097/CCE.0000000000000284"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17663"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/92088"],["dc.language.iso","en"],["dc.notes.intern","Migrated from goescholar"],["dc.relation.eissn","2639-8028"],["dc.relation.orgunit","Universitätsmedizin Göttingen"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.ddc","610"],["dc.title","Camostat Mesylate May Reduce Severity of Coronavirus Disease 2019 Sepsis: A First Observation"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e0152134"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","PloS one"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Hoffmann, Markus"],["dc.contributor.author","Krüger, Nadine"],["dc.contributor.author","Zmora, Pawel"],["dc.contributor.author","Wrensch, Florian"],["dc.contributor.author","Herrler, Georg"],["dc.contributor.author","Pöhlmann, Stefan"],["dc.date.accessioned","2019-07-09T11:42:14Z"],["dc.date.available","2019-07-09T11:42:14Z"],["dc.date.issued","2016"],["dc.description.abstract","New World bats have recently been discovered to harbor influenza A virus (FLUAV)-related viruses, termed bat-associated influenza A-like viruses (batFLUAV). The internal proteins of batFLUAV are functional in mammalian cells. In contrast, no biological functionality could be demonstrated for the surface proteins, hemagglutinin (HA)-like (HAL) and neuraminidase (NA)-like (NAL), and these proteins need to be replaced by their human counterparts to allow spread of batFLUAV in human cells. Here, we employed rhabdoviral vectors to study the role of HAL and NAL in viral entry. Vectors pseudotyped with batFLUAV-HAL and -NAL were able to enter bat cells but not cells from other mammalian species. Host cell entry was mediated by HAL and was dependent on prior proteolytic activation of HAL and endosomal low pH. In contrast, sialic acids were dispensable for HAL-driven entry. Finally, the type II transmembrane serine protease TMPRSS2 was able to activate HAL for cell entry indicating that batFLUAV can utilize human proteases for HAL activation. Collectively, these results identify viral and cellular factors governing host cell entry driven by batFLUAV surface proteins. They suggest that the absence of a functional receptor precludes entry of batFLUAV into human cells while other prerequisites for entry, HAL activation and protonation, are met in target cells of human origin."],["dc.identifier.doi","10.1371/journal.pone.0152134"],["dc.identifier.pmid","27028521"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13149"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/58625"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","The Hemagglutinin of Bat-Associated Influenza Viruses Is Activated by TMPRSS2 for pH-Dependent Entry into Bat but Not Human Cells."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","4738"],["dc.bibliographiccitation.issue","15"],["dc.bibliographiccitation.journal","Angewandte Chemie International Edition"],["dc.bibliographiccitation.lastpage","4742"],["dc.bibliographiccitation.volume","55"],["dc.contributor.author","Guo, Yuan"],["dc.contributor.author","Sakonsinsiri, Chadamas"],["dc.contributor.author","Nehlmeier, Inga"],["dc.contributor.author","Fascione, Martin A."],["dc.contributor.author","Zhang, Haiyan"],["dc.contributor.author","Wang, Weili"],["dc.contributor.author","Pöhlmann, Stefan"],["dc.contributor.author","Turnbull, W. Bruce"],["dc.contributor.author","Zhou, Dejian"],["dc.date.accessioned","2022-10-06T13:34:57Z"],["dc.date.available","2022-10-06T13:34:57Z"],["dc.date.issued","2016"],["dc.identifier.doi","10.1002/anie.201600593"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/116020"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.issn","1433-7851"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.title","Compact, Polyvalent Mannose Quantum Dots as Sensitive, Ratiometric FRET Probes for Multivalent Protein-Ligand Interactions"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

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[["dc.bibliographiccitation.firstpage","275"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Viruses"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Zapatero-Belinchón, Francisco"],["dc.contributor.author","Dietzel, Erik"],["dc.contributor.author","Dolnik, Olga"],["dc.contributor.author","Döhner, Katinka"],["dc.contributor.author","Costa, Rui"],["dc.contributor.author","Hertel, Barbara"],["dc.contributor.author","Veselkova, Barbora"],["dc.contributor.author","Kirui, Jared"],["dc.contributor.author","Klintworth, Anneke"],["dc.contributor.author","Manns, Michael"],["dc.contributor.author","Pöhlmann, Stefan"],["dc.contributor.author","Pietschmann, Thomas"],["dc.contributor.author","Krey, Thomas"],["dc.contributor.author","Ciesek, Sandra"],["dc.contributor.author","Gerold, Gisa"],["dc.contributor.author","Sodeik, Beate"],["dc.contributor.author","Becker, Stephan"],["dc.contributor.author","von Hahn, Thomas"],["dc.date.accessioned","2020-12-10T18:47:24Z"],["dc.date.available","2020-12-10T18:47:24Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.3390/v11030275"],["dc.identifier.eissn","1999-4915"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/78753"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Characterization of the Filovirus-Resistant Cell Line SH-SY5Y Reveals Redundant Role of Cell Surface Entry Factors"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1654"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Viruses"],["dc.bibliographiccitation.lastpage","1671"],["dc.bibliographiccitation.volume","6"],["dc.contributor.author","Gnirß, Kerstin"],["dc.contributor.author","Fiedler, Marie"],["dc.contributor.author","Krämer-Kühl, Annika"],["dc.contributor.author","Bolduan, Sebastian"],["dc.contributor.author","Mittler, Eva"],["dc.contributor.author","Becker, Stephan"],["dc.contributor.author","Schindler, Michael"],["dc.contributor.author","Pöhlmann, Stefan"],["dc.date.accessioned","2022-10-06T13:26:51Z"],["dc.date.available","2022-10-06T13:26:51Z"],["dc.date.issued","2014"],["dc.identifier.doi","10.3390/v6041654"],["dc.identifier.pii","v6041654"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/115181"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.eissn","1999-4915"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.title","Analysis of Determinants in Filovirus Glycoproteins Required for Tetherin Antagonism"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2034"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","Emerging Infectious Diseases"],["dc.bibliographiccitation.lastpage","2036"],["dc.bibliographiccitation.volume","19"],["dc.contributor.author","Gierer, Stefanie"],["dc.contributor.author","Hofmann-Winkler, Heike"],["dc.contributor.author","Albuali, Waleed H."],["dc.contributor.author","Bertram, Stephanie"],["dc.contributor.author","Al-Rubaish, Abdullah M."],["dc.contributor.author","Yousef, Abdullah A."],["dc.contributor.author","Al-Nafaie, Awatif N."],["dc.contributor.author","Al-Ali, Amein K."],["dc.contributor.author","Obeid, Obeid E."],["dc.contributor.author","Alkharsah, Khaled R."],["dc.contributor.author","Pöhlmann, Stefan"],["dc.date.accessioned","2022-10-06T13:26:39Z"],["dc.date.available","2022-10-06T13:26:39Z"],["dc.date.issued","2013"],["dc.identifier.doi","10.3201/eid1912.130701"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/115131"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.eissn","1080-6059"],["dc.relation.issn","1080-6040"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.title","Lack of MERS Coronavirus Neutralizing Antibodies in Humans, Eastern Province, Saudi Arabia"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","S840"],["dc.bibliographiccitation.issue","suppl_3"],["dc.bibliographiccitation.journal","The Journal of Infectious Diseases"],["dc.bibliographiccitation.lastpage","S849"],["dc.bibliographiccitation.volume","204"],["dc.contributor.author","Kühl, Annika"],["dc.contributor.author","Hoffmann, Markus"],["dc.contributor.author","Müller, Marcel A."],["dc.contributor.author","Munster, Vincent J."],["dc.contributor.author","Gnirß, Kerstin"],["dc.contributor.author","Kiene, Miriam"],["dc.contributor.author","Tsegaye, Theodros Solomon"],["dc.contributor.author","Behrens, Georg"],["dc.contributor.author","Herrler, Georg"],["dc.contributor.author","Feldmann, Heinz"],["dc.contributor.author","Pöhlmann, Stefan"],["dc.date.accessioned","2022-10-06T13:34:58Z"],["dc.date.available","2022-10-06T13:34:58Z"],["dc.date.issued","2011"],["dc.identifier.doi","10.1093/infdis/jir306"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/116022"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.eissn","1537-6613"],["dc.relation.issn","0022-1899"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.title","Comparative Analysis of Ebola Virus Glycoprotein Interactions With Human and Bat Cells"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]