Schild, Detlev

[["dc.bibliographiccitation.firstpage","3294"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Biophysical journal"],["dc.bibliographiccitation.lastpage","3306"],["dc.bibliographiccitation.volume","79"],["dc.contributor.author","Gennerich, A."],["dc.contributor.author","Schild, D."],["dc.date.accessioned","2014-02-18T08:27:34Z"],["dc.date.accessioned","2021-10-27T13:20:06Z"],["dc.date.available","2014-02-18T08:27:34Z"],["dc.date.available","2021-10-27T13:20:06Z"],["dc.date.issued","2000-12-01"],["dc.description.abstract","Fluorescence correlation spectroscopy (FCS) is a powerful technique for measuring low concentrations of fluorescent molecules and their diffusion constants. In the standard case, fluorescence fluctuations are measured in an open detection volume defined by the confocal optics. However, if FCS measurements are carried out in cellular processes that confine the detection volume, the standard FCS model leads to erroneous results. In this paper, we derive a modified FCS model that takes into account the confinement of the detection volume. Using this model, we have carried out the first FCS measurements in dendrites of cultured neurons. We further derive, for the case of confined diffusion, the limits within which the standard two- and three-dimensional diffusion models give reliable results."],["dc.identifier.doi","10.1016/S0006-3495(00)76561-1"],["dc.identifier.fs","2144"],["dc.identifier.isi","000165749000045"],["dc.identifier.pmid","11106632"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9906"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/91938"],["dc.language.iso","en"],["dc.notes.intern","Migrated from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Biophysical Society"],["dc.relation.issn","0006-3495"],["dc.relation.orgunit","Universitätsmedizin Göttingen"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.mesh","Base Sequence"],["dc.subject.mesh","DNA, Mitochondrial"],["dc.subject.mesh","Deafness"],["dc.subject.mesh","Deoxyadenine Nucleotides"],["dc.subject.mesh","Deoxyguanine Nucleotides"],["dc.subject.mesh","Genetic Testing"],["dc.subject.mesh","Hemoglobin A"],["dc.subject.mesh","Hemoglobin, Sickle"],["dc.subject.mesh","Humans"],["dc.subject.mesh","Molecular Sequence Data"],["dc.subject.mesh","Mutagenesis, Site-Directed"],["dc.subject.mesh","Oligodeoxyribonucleotides"],["dc.subject.mesh","Phosphorus Radioisotopes"],["dc.subject.mesh","Point Mutation"],["dc.subject.mesh","Polymerase Chain Reaction"],["dc.subject.mesh","Polymorphism, Genetic"],["dc.title","Fluorescence correlation spectroscopy in small cytosolic compartments depends critically on the diffusion model used"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","129"],["dc.bibliographiccitation.issue","1-2"],["dc.bibliographiccitation.journal","Journal of Neuroscience Methods"],["dc.bibliographiccitation.lastpage","135"],["dc.bibliographiccitation.volume","99"],["dc.contributor.author","Peters, F."],["dc.contributor.author","Gennerich, A."],["dc.contributor.author","Czesnik, D."],["dc.contributor.author","Schild, Detlev"],["dc.date.accessioned","2018-11-07T10:43:42Z"],["dc.date.available","2018-11-07T10:43:42Z"],["dc.date.issued","2000"],["dc.description.abstract","We implemented a simple feedback system that modifies the conventional current clamp mode of a patch clamp amplifier so that transient potentials, such as action potentials and synaptic potentials, can be measured as in the usual current clamp, while the average membrane potential is kept constant at a value chosen by the user. The circuit thus works like the current clamp for high frequency signals and like a voltage clamp for low frequency signals. We delineate its transfer properties and give application examples. (C) 2000 Elsevier Science B.V. All rights reserved."],["dc.identifier.doi","10.1016/S0165-0270(00)00223-5"],["dc.identifier.isi","000088444700016"],["dc.identifier.pmid","10936652"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9904"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/47114"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Bv"],["dc.relation.issn","0165-0270"],["dc.title","Low frequency voltage clamp: recording of voltage transients at constant average command voltage"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","181"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","European Biophysics Journal"],["dc.bibliographiccitation.lastpage","199"],["dc.bibliographiccitation.volume","34"],["dc.contributor.author","Gennerich, A."],["dc.contributor.author","Schild, Detlev"],["dc.date.accessioned","2018-11-07T11:02:48Z"],["dc.date.available","2018-11-07T11:02:48Z"],["dc.date.issued","2005"],["dc.description.abstract","Determining the positions, shapes and sizes of finite living particles such as bacteria, mitochondria or vesicles is of interest in many biological processes. In fluorescence microscopy, algorithms that can simultaneously localize such particles as a function of time and determine the parameters of their shapes and sizes at the nanometer scale are not yet available. Here we develop two such algorithms based on convolution and correlation image analysis that take into account the position, orientation, shape and size of the object being tracked, and we compare the precision of the two algorithms using computer simulations. We show that the precision of both algorithms strongly depends on the objects size. In cases where the diameter of the object is larger than about four to five times the beam waist radius, the convolution algorithm gives a better precision than the correlation algorithm (it leads to more precise parameters), while for smaller object diameters, the correlation algorithm gives superior precision. We apply the convolution algorithm to sequences of confocal laser scanning micrographs of immobile Escherichia coli bacteria, and show that the centroid, the front end, the rear end, the left border and the right border of a bacterium can be determined with a signal-to-noise-dependent precision down to ∼ 5 nm."],["dc.identifier.doi","10.1007/s00249-004-0441-0"],["dc.identifier.isi","000228859400001"],["dc.identifier.pmid","15609049"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7754"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/51471"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","0175-7571"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Sizing-up finite fluorescent particles with nanometer-scale precision by convolution and correlation image analysis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","45"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Physical Biology"],["dc.bibliographiccitation.lastpage","53"],["dc.bibliographiccitation.volume","3"],["dc.contributor.author","Gennerich, A."],["dc.contributor.author","Schild, Detlev"],["dc.date.accessioned","2018-11-07T10:15:40Z"],["dc.date.available","2018-11-07T10:15:40Z"],["dc.date.issued","2006"],["dc.description.abstract","The mechanisms of molecular motor regulation during bidirectional organelle transport are still uncertain. There is, for instance, the unsettled question of whether opposing motor proteins can be engaged in a tug-of-war. Clearly, any non-synchronous activation of the molecular motors of one cargo can principally lead to changes in the cargo's shape and size; the cargo's size and shape parameters would certainly be observables of such changes. We therefore set out to measure position, shape and size parameters of fluorescent mitochondria (during their transport) in dendrites of cultured neurons using a finite-particle tracking algorithm. Our data clearly show transport-related submicroscopic-size changes of mitochondria. The observed displacements of the mitochondrial front and rear ends are consistent with a model in which microtubule plus- and minus-end-directed motor proteins or motors of the same type but moving along anti-parallel microtubules are often out-of-phase and occasionally engaged in a tug-of-war. Mostly the leading and trailing ends of mitochondria undergo similar characteristic movements but with a substantial time delay between the displacements of both ends, a feature reminiscent of an inchworm-like motility mechanism. More generally, we demonstrate that observing the position, shape and size of actively transported finite objects such as mitochondria can yield information on organelle transport that is generally not accessible by tracking the organelles' centroid alone."],["dc.identifier.doi","10.1088/1478-3975/3/1/005"],["dc.identifier.isi","000236540400014"],["dc.identifier.pmid","16582469"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7755"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/40858"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Iop Publishing Ltd"],["dc.relation.issn","1478-3967"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Finite-particle tracking reveals submicroscopic-size changes of mitochondria during transport in mitral cell dendrites"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]