Friedl, Thomas

[["dc.bibliographiccitation.firstpage","359"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","CRYOLETTERS"],["dc.bibliographiccitation.lastpage","376"],["dc.bibliographiccitation.volume","28"],["dc.contributor.author","Day, John G."],["dc.contributor.author","Lorenz, Maike"],["dc.contributor.author","Wilding, Thomas A."],["dc.contributor.author","Friedl, Thomas"],["dc.contributor.author","Harding, Keith"],["dc.contributor.author","Prochold, Thomas"],["dc.contributor.author","Brennan, Debra"],["dc.contributor.author","Mueller, Julia"],["dc.contributor.author","Santos, Lilia M. A."],["dc.contributor.author","Santos, M. Fatima"],["dc.contributor.author","Osorio, Hugo C."],["dc.contributor.author","Amaral, Raquel"],["dc.contributor.author","Lukesova, Alena"],["dc.contributor.author","Hrouzek, Pavel"],["dc.contributor.author","Lukes, Martin"],["dc.contributor.author","Elster, Josef"],["dc.contributor.author","Lukavsky, Jaromir"],["dc.contributor.author","Probert, Ian"],["dc.contributor.author","Ryan, Matthew J."],["dc.contributor.author","Benson, Erica E."],["dc.date.accessioned","2018-11-07T10:58:50Z"],["dc.date.available","2018-11-07T10:58:50Z"],["dc.date.issued","2007"],["dc.description.abstract","Two cryopreservation methods, colligative cryoprotection coupled with controlled cooling and vitrification-based, encapsulation-dehydration were validated by five members of the EU Research Infrastructure consortium, COBRA, and two independent external validators. The test strain Chlorella vulgaris SAG 211-11b was successfully cryopreserved using two-step cooling employing passive (Mr Frosty((R))) and Controlled Rate Freezers (CRF) attaining the desired recovery target within 15% of the median viability level (94%). Significant differences (P<0.05) between cooling regimes were observed where Mr Frosty((R)) was more variable (Inter-Quartile Range being 21.5%, versus 13.0% for CRF samples). Viability assessment using fluorescein diacetate gave significantly (P<0.0001) higher survival than growth in agar with median values being 96% and 89%, respectively. On employing encapsulation-dehydration, greater variability between some validators was observed, with six labs observing recovery in 100% of the beads (84-95% of cells surviving) and one lab observing survival in 80% of the treated beads. Bead disruption followed by algal growth in agar was considered the most reliable and accurate method of assessing cell survival for encapsulation-dehydration."],["dc.identifier.isi","000250540800005"],["dc.identifier.pmid","18075705"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/50557"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cryo Letters"],["dc.relation.issn","0143-2044"],["dc.title","The use of physical and virtual infrastructures for the validation of algal cryopreservation methods in international culture collections"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","921"],["dc.bibliographiccitation.journal","Frontiers in Physiology"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Bajerski, Felizitas"],["dc.contributor.author","Stock, Johanna"],["dc.contributor.author","Hanf, Benjamin"],["dc.contributor.author","Darienko, Tatyana"],["dc.contributor.author","Heine-Dobbernack, Elke"],["dc.contributor.author","Lorenz, Maike"],["dc.contributor.author","Naujox, Lisa"],["dc.contributor.author","Keller, E. R. J."],["dc.contributor.author","Schumacher, H. M."],["dc.contributor.author","Friedl, Thomas"],["dc.contributor.author","Eberth, Sonja"],["dc.contributor.author","Mock, Hans-Peter"],["dc.contributor.author","Kniemeyer, Olaf"],["dc.contributor.author","Overmann, Jörg"],["dc.date.accessioned","2019-07-22T14:46:42Z"],["dc.date.available","2019-07-22T14:46:42Z"],["dc.date.issued","2018"],["dc.description.abstract","In many natural environments, organisms get exposed to low temperature and/or to strong temperature shifts. Also, standard preservation protocols for live cells or tissues involve ultradeep freezing in or above liquid nitrogen (-196°C or -150°C, respectively). To which extent these conditions cause cold- or cryostress has rarely been investigated systematically. Using ATP content as an indicator of the physiological state of cells, we found that representatives of bacteria, fungi, algae, plant tissue, as well as plant and human cell lines exhibited similar responses during freezing and thawing. Compared to optimum growth conditions, the cellular ATP content of most model organisms decreased significantly upon treatment with cryoprotectant and cooling to up to -196°C. After thawing and a longer period of regeneration, the initial ATP content was restored or even exceeded the initial ATP levels. To assess the implications of cellular ATP concentration for the physiology of cryostress, cell viability was determined in parallel using independent approaches. A significantly positive correlation of ATP content and viability was detected only in the cryosensitive algae Chlamydomonas reinhardtii SAG 11-32b and Chlorella variabilis NC64A, and in plant cell lines of Solanum tuberosum. When comparing mesophilic with psychrophilic bacteria of the same genera, and cryosensitive with cryotolerant algae, ATP levels of actively growing cells were generally higher in the psychrophilic and cryotolerant representatives. During exposure to ultralow temperatures, however, psychrophilic and cryotolerant species showed a decline in ATP content similar to their mesophilic or cryosensitive counterparts. Nevertheless, psychrophilic and cryotolerant species attained better culturability after freezing. Cellular ATP concentrations and viability measurements thus monitor different features of live cells during their exposure to ultralow temperatures and cryostress."],["dc.identifier.doi","10.3389/fphys.2018.00921"],["dc.identifier.pmid","30065659"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/61835"],["dc.language.iso","en"],["dc.notes.intern","DeepGreen Import"],["dc.publisher","Frontiers Media S.A."],["dc.relation.eissn","1664-042X"],["dc.relation.issn","1664-042X"],["dc.rights","http://creativecommons.org/licenses/by/4.0/"],["dc.title","ATP Content and Cell Viability as Indicators for Cryostress Across the Diversity of Life"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","460"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","CRYOLETTERS"],["dc.bibliographiccitation.lastpage","472"],["dc.bibliographiccitation.volume","31"],["dc.contributor.author","Harding, Keith"],["dc.contributor.author","Mueller, Julia"],["dc.contributor.author","Timmermann, Hella"],["dc.contributor.author","Lorenz, Maike"],["dc.contributor.author","Day, John G."],["dc.contributor.author","Friedl, Thomas"],["dc.date.accessioned","2018-11-07T08:37:25Z"],["dc.date.available","2018-11-07T08:37:25Z"],["dc.date.issued","2010"],["dc.description.abstract","An encapsulation/dehydration procedure was developed for Euglena gracilis Klebs as a 'model alga' to examine various cryoprotective regimes combined with controlled rate cooling to cryopreserve other Euglenoid taxa. Cryoprotective variables were optimised to enable reproducible growth following a combination of alginate encapsulation, sucrose osmotic dehydration, air desiccation, methanol treatment, cooling to -40 degrees C and plunging into liquid nitrogen (LN). Amplified Fragment Length Polymorphism (AFLP) analysis was adapted to: (i) verify algal identity by discriminating between different Euglenoids and (ii) examine the genetic stability of algal cultures prior to various stages of cryoprotective treatments and following exposure to LN. AFLPs were highly reproducible (>99%) as reliable diagnostic markers, where a single DNA fragment change accounted for similar to 0.4% of the detectable variation in an AFLP pattern. AFLP changes were detected in cryoprotective treatments following LN exposure. Successive stages of the dehydration and desiccation treatments did not accumulate AFLP changes indicating these are random events."],["dc.identifier.isi","000286697900004"],["dc.identifier.pmid","21410015"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/18528"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cryo Letters"],["dc.relation.issn","0143-2044"],["dc.title","ENCAPSULATION DEHYDRATION COLLIGATIVE CRYOPROTECTIVE STRATEGIES AND AMPLIFIED FRAGMENT LENGTH POLYMORPHISM MARKERS TO VERIFY THE IDENTITY AND GENETIC STABILITY OF EUGLENOIDS FOLLOWING CRYOPRESERVATION"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","125697"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Protist"],["dc.bibliographiccitation.volume","170"],["dc.contributor.author","Wang, Sibo"],["dc.contributor.author","Li, Linzhou"],["dc.contributor.author","Xu, Yan"],["dc.contributor.author","Melkonian, Barbara"],["dc.contributor.author","Lorenz, Maike"],["dc.contributor.author","Friedl, Thomas"],["dc.contributor.author","Petersen, Morten"],["dc.contributor.author","Sahu, Sunil Kumar"],["dc.contributor.author","Melkonian, Michael"],["dc.contributor.author","Liu, Huan"],["dc.date.accessioned","2020-12-10T15:20:56Z"],["dc.date.available","2020-12-10T15:20:56Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1016/j.protis.2019.125697"],["dc.identifier.issn","1434-4610"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/72861"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","The Draft Genome of the Small, Spineless Green Alga Desmodesmus costato-granulatus (Sphaeropleales, Chlorophyta)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","207"],["dc.bibliographiccitation.issue","1-2"],["dc.bibliographiccitation.journal","Nova Hedwigia"],["dc.bibliographiccitation.lastpage","226"],["dc.bibliographiccitation.volume","79"],["dc.contributor.author","Harding, Keith"],["dc.contributor.author","Day, John G."],["dc.contributor.author","Lorenz, Maike"],["dc.contributor.author","Timmermann, Hella"],["dc.contributor.author","Friedl, Thomas"],["dc.contributor.author","Bremner, David H."],["dc.contributor.author","Benson, Erica E."],["dc.date.accessioned","2018-11-07T10:46:59Z"],["dc.date.available","2018-11-07T10:46:59Z"],["dc.date.issued","2004"],["dc.description.abstract","This review pertains to a project (COBRA QLRT-2000-01645, http://www.cobra.ac.uk) supported by the European Commission's Fifth Framework Programme for Quality of Life and Management of Living Resources. The aim of this initiative is to develop and apply novel cryo-preservation protocols to microalgae and cyanobacteria that are difficult to cryopreserve. One such approach utilizes cryoprotective vitrification for the conservation of algal culture collections at -196degreesC in liquid nitrogen. Achieving a vitrified state depends on increasing cellular viscosity to a critical point at which water forms an amorphous, non-crystalline glass on cooling to ultra-low temperatures. Vitrification has been utilized extensively in medical cryobiology and for the preservation of animal, human and higher plant germplasm but its application for the conservation of algae and cyarlobacteria is less widespread. The review provides a theoretical introduction to vitrification and highlights current and potential applications in algal preservation, especially for storage-recalcitrant organisms."],["dc.identifier.doi","10.1127/0029-5035/2004/0079-0207"],["dc.identifier.isi","000223912300017"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/47865"],["dc.language.iso","en"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Gebruder Borntraeger"],["dc.publisher.place","Stuttgart"],["dc.relation.conference","International Symposium on Culture Collections of Algae"],["dc.relation.eventlocation","Univ Gottingen, Gottingen, GERMANY"],["dc.relation.issn","0029-5035"],["dc.title","Introducing the concept and application of vitrification for the cryo-conservation of algae - a mini-review"],["dc.type","conference_paper"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","204"],["dc.bibliographiccitation.journal","European Journal of Phycology"],["dc.bibliographiccitation.lastpage","205"],["dc.bibliographiccitation.volume","50"],["dc.contributor.author","Darienko, Tatyana"],["dc.contributor.author","Siegesmund, Maria A."],["dc.contributor.author","Lorenz, Maike"],["dc.contributor.author","Rybalka, Nataliya"],["dc.contributor.author","Friedl, Thomas"],["dc.date.accessioned","2018-11-07T10:03:01Z"],["dc.date.available","2018-11-07T10:03:01Z"],["dc.date.issued","2015"],["dc.identifier.isi","000360244400382"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/38353"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Taylor & Francis Ltd"],["dc.publisher.place","Abingdon"],["dc.relation.issn","1469-4433"],["dc.relation.issn","0967-0262"],["dc.title","EFFECTS OF CRYOPRESERVATION ON SELECTED GREEN MICROALGAE USING AFLP FINGERPRINTING FOR GENOMIC AND EPIGENOMIC STABILITY ASSESSMENT"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","110"],["dc.bibliographiccitation.journal","Procedia Environmental Sciences"],["dc.bibliographiccitation.lastpage","117"],["dc.bibliographiccitation.volume","15"],["dc.contributor.author","Friedl, Thomas"],["dc.contributor.author","Lorenz, Maike"],["dc.date.accessioned","2019-07-09T11:40:59Z"],["dc.date.available","2019-07-09T11:40:59Z"],["dc.date.issued","2012"],["dc.description.abstract","The SAG is one of the most comprehensive resources of microalgal cultures (www.epsag.uni-goettingen.de). It is supporting research in biotechnology and biodiversity through ex situ conservation of algae and expert knowledge on identifying and isolating. Multiple strains proven to represent the same microalgal species exhibit extensive genotypic diversity interesting for further exploitation. Cryopreservation is well suited to circumvent problems associated with perpetual maintenance, but needs optimization to ensure genetic stability. To ensure the SAG's reliability, primary goals are correctly identified strains as references for DNA sequence comparisons. Novel isolates from unusual terrestrial habitats worth further biotechnological exploitation are being developed."],["dc.identifier.doi","10.1016/j.proenv.2012.05.015"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/11516"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/58328"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1878-0296"],["dc.rights","CC BY-NC-ND 3.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc-nd/3.0"],["dc.title","The Culture Collection of Algae at Göttingen University (SAG): A Biological Resource for Biotechnological and Biodiversity Research"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","799"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","American Journal of Botany"],["dc.bibliographiccitation.lastpage","808"],["dc.bibliographiccitation.volume","94"],["dc.contributor.author","Mueller, Julia"],["dc.contributor.author","Day, John G."],["dc.contributor.author","Harding, Keith"],["dc.contributor.author","Hepperle, Dominik"],["dc.contributor.author","Lorenz, Maike"],["dc.contributor.author","Friedl, Thomas"],["dc.date.accessioned","2018-11-07T11:02:31Z"],["dc.date.available","2018-11-07T11:02:31Z"],["dc.date.issued","2007"],["dc.description.abstract","Cryopreservation is the long-term, indefinite storage of living biological resources at ultralow temperatures. It is almost universally assumed that cryogenic storage supports genetic and phenotypic stability of organisms. However, certain components of the cryopreservation process, particularly some cryoprotective additives (CPAs) and free radical mediated cryoinjury, may potentially cause genetic alterations. Genetic integrity in cryopreserved microalgae was assessed using a very sensitive molecular fingerprinting technique, AFLP, on 28 terrestrial microalgal strains. In about half of all investigated strains the AFLP fingerprints revealed, with high levels of reproducibility, clearly detectable genomic differences after cryopreservation employing a widely used standard two-step cooling protocol. Differences ranged from a single fragment position to multiple fragment changes and were compared to differences found between wild-type and UV-light- or radioisotope-induced mutants of Parachlorella kessleri. The basis of the changes are discussed in terms of their reversibility, as may be the case if they are attributed to DNA methylation and/or whether they are true mutations that may potentially manifest in the phenotype. The possibility that cryopreservation selects for genotypically different subpopulations of microalgae is also considered."],["dc.identifier.doi","10.3732/ajb.94.5.799"],["dc.identifier.isi","000249830400011"],["dc.identifier.pmid","21636449"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/51401"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Botanical Soc Amer Inc"],["dc.relation.issn","0002-9122"],["dc.title","Assessing genetic stability of a range of terrestrial microalgae after cryopreservation using amplified fragment length polymorphism (AFLP)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","95"],["dc.bibliographiccitation.journal","European Journal of Phycology"],["dc.bibliographiccitation.lastpage","96"],["dc.bibliographiccitation.volume","46"],["dc.contributor.author","Lorenz, Maike"],["dc.contributor.author","Campbell, Christine"],["dc.contributor.author","Friedl, Thomas"],["dc.contributor.author","Day, John G."],["dc.date.accessioned","2018-11-07T09:01:12Z"],["dc.date.available","2018-11-07T09:01:12Z"],["dc.date.issued","2011"],["dc.identifier.isi","000299418700138"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/24360"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Taylor & Francis Ltd"],["dc.publisher.place","Abingdon"],["dc.relation.issn","0967-0262"],["dc.title","A EUROPEAN PERSPECTIVE ON ALGAL RESOURCES: BIODIVERSITY, BIOTECHNOLOGY AND BEYOND"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","568"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Ecology Letters"],["dc.bibliographiccitation.lastpage","569"],["dc.bibliographiccitation.volume","16"],["dc.contributor.author","Steudel, Bastian"],["dc.contributor.author","Hector, Andy"],["dc.contributor.author","Friedl, Thomas"],["dc.contributor.author","Loefke, Christian"],["dc.contributor.author","Lorenz, Maike"],["dc.contributor.author","Wesche, Moritz"],["dc.contributor.author","Kessler, Michael"],["dc.date.accessioned","2018-11-07T09:26:40Z"],["dc.date.available","2018-11-07T09:26:40Z"],["dc.date.issued","2013"],["dc.identifier.doi","10.1111/ele.12079"],["dc.identifier.isi","000316630200017"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/30348"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.relation.issn","1461-023X"],["dc.title","Biodiversity effects on ecosystem functioning change along environmental stress gradients (vol 15, pg 1397, 2012)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]