Lange, Peter

[["dc.bibliographiccitation.firstpage","126"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Dementia and Geriatric Cognitive Disorders"],["dc.bibliographiccitation.lastpage","134"],["dc.bibliographiccitation.volume","32"],["dc.contributor.author","Fronek, Kathrin"],["dc.contributor.author","Spreer, Annette"],["dc.contributor.author","Eiffert, Helmut"],["dc.contributor.author","Nau, Roland"],["dc.contributor.author","Lange, Peter"],["dc.date.accessioned","2018-11-07T08:51:09Z"],["dc.date.available","2018-11-07T08:51:09Z"],["dc.date.issued","2011"],["dc.description.abstract","Background/Aims: Determination of marker proteins of neuronal degeneration in cerebrospinal fluid (CSF) is of increasing importance. However, preanalytical problems may compromise the results. Methods: We studied the influence of the transport tube material and shaking at room temperature on the CSF concentrations of beta-amyloid and tau protein determined by enzyme immunoassays. Results: The materials of the transport tube moderately influenced the CSF concentrations of beta-amyloid and tau protein. Polyethylene and polypropylene tubes were well suited, but glass, polycarbonate and polystyrene tubes caused a decrease in the CSF beta-amyloid and tau protein concentrations. The strongest impact, however, was caused by bacterial contamination of samples. Contamination with high concentrations of Pseudomonas aeruginosa and related species rendered beta-amyloid undetectable and strongly diminished tau protein concentrations. The effects of several Gram-positive bacteria were less pronounced. Addition of 0.1% sodium azide prior to bacterial contamination increased the interval at which CSF could be kept at room temperature without a substantial reduction of the beta-amyloid or tau protein concentration. Conclusion: Polyethylene or polypropylene tubes are suitable transport vessels for CSF samples. Bacterial contamination during sampling and portioning must be avoided. Addition of sodium azide may be an option when transport of the sample is delayed. Copyright (C) 2011 S. Karger AG, Basel"],["dc.identifier.doi","10.1159/000330912"],["dc.identifier.fs","580959"],["dc.identifier.isi","000295875400006"],["dc.identifier.pmid","21952521"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/21862"],["dc.language.iso","en"],["dc.notes","This publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively."],["dc.notes.intern","Merged from goescholar"],["dc.notes.intern","In goescholar merged with http://resolver.sub.uni-goettingen.de/purl?gs-1/8159"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","S. Karger AG"],["dc.relation.eissn","1421-9824"],["dc.relation.issn","1420-8008"],["dc.rights","Goescholar"],["dc.rights.access","openAccess"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.mesh","Amyloid beta-Peptides"],["dc.subject.mesh","Cerebrospinal Fluid"],["dc.subject.mesh","Cerebrospinal Fluid Proteins"],["dc.subject.mesh","Dementia"],["dc.subject.mesh","Enzyme-Linked Immunosorbent Assay"],["dc.subject.mesh","Equipment Design"],["dc.subject.mesh","Humans"],["dc.subject.mesh","Pseudomonas aeruginosa"],["dc.subject.mesh","Quality Control"],["dc.subject.mesh","Specimen Handling"],["dc.subject.mesh","Stenotrophomonas maltophilia"],["dc.subject.mesh","tau Proteins"],["dc.title","Bacterial Contamination and the Transport Vial Material Affect Cerebrospinal Fluid Concentrations of beta-Amyloid and Tau Protein as Determined by Enzyme Immunoassay"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]