Schönle, Andreas

[["dc.bibliographiccitation.firstpage","3361"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Optics Express"],["dc.bibliographiccitation.lastpage","3371"],["dc.bibliographiccitation.volume","15"],["dc.contributor.author","Keller, Jan"],["dc.contributor.author","Schönle, Andreas"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:49:49Z"],["dc.date.available","2017-09-07T11:49:49Z"],["dc.date.issued","2007"],["dc.description.abstract","By exploiting the saturation of a reversible single photon transition, RESOLFT microscopy is capable of resolving three dimensional structures inside specimen with a resolution that is no longer limited by the wavelength of the light in use. The transition is driven by a spatially varying intensity distribution that features at least one isolated point, line or plane with zero intensity and the resolution achieved depends critically on the field distribution around these zeros. Based on a vectorial analysis of the image formation in a RESOLFT microscope, we develop a method to effectively search for optimal zero intensity point patterns under typical experimental conditions. Using this approach, we derived a spatial intensity distribution that optimizes the focal plane resolution. Moreover, we outline a general strategy that allows optimization of the resolution for a given experimental situation and present solutions for the most common cases in biological imaging."],["dc.identifier.doi","10.1364/OE.15.003361"],["dc.identifier.gro","3143520"],["dc.identifier.isi","000245076200065"],["dc.identifier.pmid","19532577"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1043"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1094-4087"],["dc.title","Efficient fluorescence inhibition patterns for RESOLFT microscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","480"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Journal of Biomedical Optics"],["dc.bibliographiccitation.lastpage","484"],["dc.bibliographiccitation.volume","6"],["dc.contributor.author","Huse, N."],["dc.contributor.author","Schönle, Andreas"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:46:03Z"],["dc.date.available","2017-09-07T11:46:03Z"],["dc.date.issued","2001"],["dc.description.abstract","In light microscopy the transverse nature of the electromagnetic field precludes a strongly focused longitudinal field component, thus confining polarization spectroscopy and imaging to two dimensions (xy). Here we describe a simple confocal microscopy arrangement that optimizes for signal from molecules with transition dipoles oriented parallel to the optic axis. In the proposed arrangement, we not only generate a predominant longitudinally (z) polarized focal field, but also engineer the detection scheme in such a way that in a bulk of randomly oriented molecules, the microscope's effective point-spread function is dominated by the contribution of those molecules that are oriented along the optic axis. Our arrangement not only implicitly allows for the determination of the orientation of transition dipoles of single molecules in three dimensions, but also highlights the contribution of z-oriented molecules in three-dimensional imaging. (C) 2001 Society of Photo-optical Instrumentation Engineers."],["dc.identifier.doi","10.1117/1.1417974"],["dc.identifier.gro","3144252"],["dc.identifier.isi","000172895700014"],["dc.identifier.pmid","11806348"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1855"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Spie-int Society Optical Engineering"],["dc.relation.issn","1083-3668"],["dc.title","Z-polarized confocal microscopy (vol 6, pg 273, 2001)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","6266"],["dc.bibliographiccitation.issue","33"],["dc.bibliographiccitation.journal","Angewandte Chemie International Edition"],["dc.bibliographiccitation.lastpage","6270"],["dc.bibliographiccitation.volume","46"],["dc.contributor.author","Fölling, Jonas"],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Kunetsky, R."],["dc.contributor.author","Medda, Rebecca"],["dc.contributor.author","Schoenle, Andreas"],["dc.contributor.author","Egner, Alexander"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Bossi, Mariano L."],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:49:52Z"],["dc.date.available","2017-09-07T11:49:52Z"],["dc.date.issued","2007"],["dc.description.abstract","Exciting developments: Switching individual photochromic and fluorescent rhodamine amides enables 3D far-field optical microscopy with nanoscale resolution, excellent signal-to-noise ratio, and fast acquisition times. The rhodamine amides can be switched on using two photons, which enables 3D detailed imaging of thick and densely stained samples (such as 5-μm silica beads (see image) and living cells) to be constructed."],["dc.identifier.doi","10.1002/anie.200702167"],["dc.identifier.gro","3143552"],["dc.identifier.isi","000249114700006"],["dc.identifier.pmid","17640007"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1078"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1433-7851"],["dc.title","Photochromic rhodamines provide nanoscopy with optical sectioning"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","131"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","FEBS Letters"],["dc.bibliographiccitation.lastpage","135"],["dc.bibliographiccitation.volume","479"],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Subramaniam, Vinod"],["dc.contributor.author","Schönle, Andreas"],["dc.contributor.author","Jovin, Thomas M."],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2017-09-07T11:46:46Z"],["dc.date.available","2017-09-07T11:46:46Z"],["dc.date.issued","2000"],["dc.description.abstract","The green fluorescent protein (GFP) has become an invaluable marker for monitoring protein localization and gene expression in vivo. Recently a new red fluorescent protein (drFP583 or DsRed), isolated from tropical corals, has been described [Matz, M.V. et al, (1999) Nature Biotech, 17, 969-973]. With emission maxima at 509 and 583 nm respectively, EGFP and DsRed are suited for almost crossover free dual color labeling upon simultaneous excitation, We imaged mixed populations of Escherichia coli expressing either EGFP or DsRed by one-photon confocal and by two-photon microscopy, Both excitation modes proved to be suitable for imaging cells expressing either of the fluorescent proteins. DsRed had an extended maturation time and E, coli expressing this fluorescent protein were significantly smaller than those expressing EGFP, In aging bacterial cultures DsRed appeared to aggregate within the cells, accompanied by a strong reduction in its fluorescence lifetime as determined by fluorescence lifetime imaging microscopy, (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved."],["dc.identifier.doi","10.1016/S0014-5793(00)01896-2"],["dc.identifier.gro","3144363"],["dc.identifier.isi","000088963900011"],["dc.identifier.pmid","10981721"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1979"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0014-5793"],["dc.title","EGFP and DsRed expressing cultures of Escherichia coli imaged by confocal, two-photon and fluorescence lifetime microscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","L67"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","L69"],["dc.bibliographiccitation.volume","92"],["dc.contributor.author","Donnert, Gerald"],["dc.contributor.author","Keller, Jan"],["dc.contributor.author","Wurm, Christian Andreas"],["dc.contributor.author","Rizzoli, Silvio"],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Schoenle, Andreas"],["dc.contributor.author","Jahn, Reinhard"],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:49:49Z"],["dc.date.available","2017-09-07T11:49:49Z"],["dc.date.issued","2007"],["dc.description.abstract","We demonstrate two-color fluorescence microscopy with nanoscale spatial resolution by applying stimulated emission depletion on fluorophores differing in their absorption and emission spectra. Green- and red-emitting fluorophores are selectively excited and quenched using dedicated beam pairs. The stimulated emission depletion beams deliver a lateral resolution of < 30 nm and 65 nm for the green and the red color channel, respectively. The similar to 5 nm alignment accuracy of the two images establishes the precision with which differently labeled proteins are correlated in space. Colocalized nanoscopy is demonstrated with endosomal protein patterns and by resolving nanoclusters of a mitochondrial outer membrane protein, Tom20, in relation with the F(1)F(0)ATP synthase. The joint improvement of resolution and colocalization demonstrates the emerging potential of far-field fluorescence nanoscopy to study the spatial organization of macromolecules in cells."],["dc.identifier.doi","10.1529/biophysj.107.104497"],["dc.identifier.gro","3143514"],["dc.identifier.isi","000245164000003"],["dc.identifier.pmid","17307826"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1037"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0006-3495"],["dc.title","Two-color far-field fluorescence nanoscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","675"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","684"],["dc.bibliographiccitation.volume","99"],["dc.contributor.author","Kamin, Dirk"],["dc.contributor.author","Lauterbach, Marcel A."],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Keller, Jan"],["dc.contributor.author","Schönle, Andreas"],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Rizzoli, Silvio"],["dc.date.accessioned","2017-09-07T11:45:20Z"],["dc.date.available","2017-09-07T11:45:20Z"],["dc.date.issued","2010"],["dc.description.abstract","Synaptic vesicles need to be mobile to reach their release sites during synaptic activity. We investigated vesicle mobility throughout the synaptic vesicle cycle using both conventional and subdiffraction-resolution stimulated emission depletion fluorescence microscopy. Vesicle tracking revealed that recently endocytosed synaptic vesicles are highly mobile for a substantial time period after endocytosis. They later undergo a maturation process and integrate into vesicle clusters where they exhibit little mobility. Despite the differences in mobility, both recently endocytosed and mature vesicles are exchanged between synapses. Electrical stimulation does not seem to affect the mobility of the two types of vesicles. After exocytosis, the vesicle material is mobile in the plasma membrane, although the movement appears to be somewhat limited. Increasing the proportion of fused vesicles (by stimulating exocytosis while simultaneously blocking endocytosis) leads to substantially higher mobility. We conclude that both high- and low-mobility states are characteristic of synaptic vesicle movement."],["dc.identifier.doi","10.1016/j.bpj.2010.04.054"],["dc.identifier.gro","3142885"],["dc.identifier.isi","000280182300042"],["dc.identifier.pmid","20643088"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/338"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0006-3495"],["dc.title","High- and Low-Mobility Stages in the Synaptic Vesicle Cycle"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","325"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Optics Letters"],["dc.bibliographiccitation.lastpage","327"],["dc.bibliographiccitation.volume","23"],["dc.contributor.author","Schönle, Andreas"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:48:11Z"],["dc.date.available","2017-09-07T11:48:11Z"],["dc.date.issued","1998"],["dc.description.abstract","We derive an integral solution for the local heating of a linearly absorbing, uniform medium exposed to strongly focused light. Numerical results for local heating under typical multiphoton microscopy and optical trapping conditions are presented for various aperture angles. In contrast with common Gaussian beam approximations, our model employs the focal-intensity distribution as described by the point spread function of the lens. In this way, the model also accounts for axial heat transportation, which results in a lower prediction for the temperature increase. For an aperture of 1.2 (water immersion), irradiation with 100 mW of 850-nm light for 1 a increases the local temperature of water by 0.2 K. Heating of water by linear absorption can be ruled out as a limiting factor in standard multiphoton-excitation microscopy. (C) 1998 Optical Society of America."],["dc.identifier.doi","10.1364/OL.23.000325"],["dc.identifier.gro","3144557"],["dc.identifier.isi","000072324400005"],["dc.identifier.pmid","18084500"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2194"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Optical Soc Amer"],["dc.relation.issn","0146-9592"],["dc.title","Heating by absorption in the focus of an objective lens"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","417"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Journal of Biophotonics"],["dc.bibliographiccitation.lastpage","424"],["dc.bibliographiccitation.volume","3"],["dc.bibliographiccitation.volumetitle","Advanced Micro and Nanoscopy for Biomedicine"],["dc.contributor.author","Lauterbach, Marcel A."],["dc.contributor.author","Keller, Jan"],["dc.contributor.author","Schönle, Andreas"],["dc.contributor.author","Kamin, Dirk"],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Rizzoli, Silvio"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:45:57Z"],["dc.date.available","2017-09-07T11:45:57Z"],["dc.date.issued","2010"],["dc.description.abstract","We compare the performance of video-rate Stimulated Emission Depletion (STED) and confocal microscopy in imaging the interior of living neurons. A lateral resolution of 65 nm is observed in STED movies of 28 frames per second, which is 4-fold higher in spatial resolution than in their confocal counterparts. S FED microscopy, but not confocal microscopy, allows discrimination of single features at high spatial densities. Specific patterns of movement within the confined space of the axon are revealed in STED microscopy, while confocal imaging is limited to reporting gross motion. Further progress is to be expected, as we demonstrate that the use of continuous wave (CW) beams for excitation and STED is viable for video-rate STED recording of living neurons. Tentatively providing a larger photon flux, CW beams should facilitate extending fast STED imaging towards imaging fainter living samples. [GRAPHICS] Synaptic vesicles within an axon. Single frames of movies recorded at 28 frames per second. Confocal microscopy (left) can only reproduce the axon, whereas STED microscopy (right) can resolve moving objects in the neuron; '+' indicates that the data are linearly deconvolved."],["dc.identifier.doi","10.1002/jbio.201000038"],["dc.identifier.gro","3142897"],["dc.identifier.isi","000279969600003"],["dc.identifier.pmid","20379984"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/352"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1864-063X"],["dc.title","Comparing video-rate STED nanoscopy and confocal microscopy of living neurons"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","480"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Journal of Biomedical Optics"],["dc.bibliographiccitation.lastpage","484"],["dc.bibliographiccitation.volume","6"],["dc.contributor.author","Huse, Nils"],["dc.contributor.author","Schönle, Andreas"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:46:05Z"],["dc.date.available","2017-09-07T11:46:05Z"],["dc.date.issued","2001"],["dc.description.abstract","In light microscopy the transverse nature of the electromagnetic field precludes a strongly focused longitudinal field component, thus confining polarization spectroscopy and imaging to two dimensions (xy). Here we describe a simple confocal microscopy arrangement that optimizes for signal from molecules with transition dipoles oriented parallel to the optic axis. In the proposed arrangement, we not only generate a predominant longitudinally (z) polarized focal field, but also engineer the detection scheme in such a way that in a bulk of randomly oriented molecules, the microscope's effective point-spread function is dominated by the contribution of those molecules that are oriented along the optic axis. Our arrangement not only implicitly allows for the determination of the orientation of transition dipoles of single molecules in three dimensions, but also highlights the contribution of z-oriented molecules in three-dimensional imaging."],["dc.identifier.doi","10.1117/1.1382610"],["dc.identifier.gro","3144274"],["dc.identifier.isi","000170405900002"],["dc.identifier.pmid","11516316"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1880"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1083-3668"],["dc.title","Z-polarized confocal microscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","OP309"],["dc.bibliographiccitation.issue","44"],["dc.bibliographiccitation.journal","Advanced Materials"],["dc.bibliographiccitation.lastpage","OP313"],["dc.bibliographiccitation.volume","24"],["dc.contributor.author","Wildanger, Dominik"],["dc.contributor.author","Patton, Brian R."],["dc.contributor.author","Schill, Heiko"],["dc.contributor.author","Marseglia, Luca"],["dc.contributor.author","Hadden, J. P."],["dc.contributor.author","Knauer, Sebastian"],["dc.contributor.author","Schönle, Andreas"],["dc.contributor.author","Rarity, John G."],["dc.contributor.author","O'Brien, J. L."],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Smith, Jason M."],["dc.date.accessioned","2017-09-07T11:48:21Z"],["dc.date.available","2017-09-07T11:48:21Z"],["dc.date.issued","2012"],["dc.description.abstract","Exploring the maximum spatial resolution achievable in far-field optical imaging, we show that applying solid immersion lenses (SIL) in stimulated emission depletion (STED) microscopy addresses single spins with a resolution down to 2.4 +/- 0.3 nm and with a localization precision of 0.09 nm."],["dc.identifier.doi","10.1002/adma.201203033"],["dc.identifier.gro","3142439"],["dc.identifier.isi","000311699100004"],["dc.identifier.pmid","22968917"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/8296"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0935-9648"],["dc.title","Solid Immersion Facilitates Fluorescence Microscopy with Nanometer Resolution and Sub-Ångström Emitter Localization"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]