Opitz, Lennart

[["dc.bibliographiccitation.firstpage","3400"],["dc.bibliographiccitation.journal","Journal of General Virology"],["dc.bibliographiccitation.lastpage","3412"],["dc.bibliographiccitation.volume","97"],["dc.contributor.author","Javed, Aneela"],["dc.contributor.author","Leuchte, Nicole"],["dc.contributor.author","Salinas, Gabriela"],["dc.contributor.author","Opitz, Lennart"],["dc.contributor.author","Stahl-Hennig, Christiane"],["dc.contributor.author","Sopper, Sieghart"],["dc.contributor.author","Sauermann, Ulrike"],["dc.date.accessioned","2018-11-07T10:05:02Z"],["dc.date.available","2018-11-07T10:05:02Z"],["dc.date.issued","2016"],["dc.description.abstract","CD8(+) cells from simian immunodeficiency virus (SIV)-infected long-term non-progressors and some uninfected macaques can suppress viral replication in vitro without killing the infected cells. The aim of this study was to identify factors responsible for non-cytolytic viral suppression by transcriptional profiling and to investigate their potential impact on SIV replication. Results of microarray experiments and further validation with cells from infected and uninfected macaques revealed that FAM26F RNA levels distinguished CD8(+) cells of controllers and non-controllers (P=0.001). However, FAM26F was also expressed in CD4(+) T-cells and B-cells. FAM26F expression increased in lymphocytes after in vitro IFN-gamma treatment on average 40-fold, and ex vivo FAM26F RNA levels in peripheral blood mononuclear cells correlated with plasma IFN-gamma but not with IFN-alpha. Baseline FAM26F expression appeared to be stable for months, albeit the individual expression levels varied up to tenfold. Investigating its role in SIV-infection revealed that FAM26F was upregulated after infection (P<0.0008), but did not directly correlate with viral load in contrast to MX1 and CXCL10. However, pre-infection levels of FAM26F correlated inversely with overall plasma viral load (AUC) during the acute and post-acute phases of infection (e.g. AUC weeks post infection 0-8; no AIDS vaccine: P<0.0001, Spearman rank correlation coefficient (rs)=-0.89, n=16; immunized with an AIDS vaccine: P=0.033, rs=-0.43; n=25). FAM26F transcript levels prior to infection can provide information about the pace and strength of the antiviral immune response during the early stage of infection. FAM26F expression represented, in our experiments, one of the earliest prognostic markers, and could supplement major histocompatibility complex (MHC)-typing to predict disease progression before SIV-infection."],["dc.identifier.doi","10.1099/jgv.0.000632"],["dc.identifier.isi","000390684000030"],["dc.identifier.pmid","27902344"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14305"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/38817"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Microbiology Soc"],["dc.relation.issn","1465-2099"],["dc.relation.issn","0022-1317"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Pre-infection transcript levels of FAM26F in peripheral blood mononuclear cells inform about overall plasma viral load in acute and post-acute phase after simian immunodeficiency virus infection"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Wiener klinische Wochenschrift"],["dc.bibliographiccitation.volume","120"],["dc.contributor.author","Novota, P."],["dc.contributor.author","Sviland, Lisbet"],["dc.contributor.author","Opitz, Lennart"],["dc.contributor.author","Hitt, Reiner"],["dc.contributor.author","Dickinson, Anne M."],["dc.contributor.author","Walter, L."],["dc.contributor.author","Dressel, Ralf"],["dc.date.accessioned","2018-11-07T11:12:51Z"],["dc.date.available","2018-11-07T11:12:51Z"],["dc.date.issued","2008"],["dc.format.extent","124"],["dc.identifier.isi","000259367100404"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/53754"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.publisher.place","Wien"],["dc.relation.issn","0043-5325"],["dc.title","Major histocompatibility complex (MHC) gene expression profiling of the graft versus host reaction in skin explant assays"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","251"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Molecular and Cellular Biology"],["dc.bibliographiccitation.lastpage","265"],["dc.bibliographiccitation.volume","32"],["dc.contributor.author","Studencka, Maja"],["dc.contributor.author","Konzer, Anne"],["dc.contributor.author","Moneron, Gael"],["dc.contributor.author","Wenzel, Dirk"],["dc.contributor.author","Opitz, Lennart"],["dc.contributor.author","Salinas-Riester, Gabriela"],["dc.contributor.author","Bedet, Cecile"],["dc.contributor.author","Krüger, Marcus"],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Wisniewski, Jacek R."],["dc.contributor.author","Schmidt, Henning"],["dc.contributor.author","Palladino, Francesca"],["dc.contributor.author","Schulze, Ekkehard"],["dc.contributor.author","Jedrusik-Bode, Monika"],["dc.date.accessioned","2017-09-07T11:43:14Z"],["dc.date.available","2017-09-07T11:43:14Z"],["dc.date.issued","2012"],["dc.description.abstract","Linker histone (H1) and heterochromatin protein 1 (HP1) are essential components of heterochromatin which contribute to the transcriptional repression of genes. It has been shown that the methylation mark of vertebrate histone HI is specifically recognized by the chromodomain of HP1. However, the exact biological role of linker histone binding to HP1 has not been determined. Here, we investigate the function of the Caenorhabditis elegans H1 variant HIS-24 and the HP1-like proteins HPL-1 and HPL-2 in the cooperative transcriptional regulation of immune-relevant genes. We provide the first evidence that HPL-1 interacts with HIS-24 monomethylated at lysine 14 (HIS-24KI4me1) and associates in vivo with promoters of genes involved in antimicrobial response. We also report an increase in overall cellular levels and alterations in the distribution of HIS-24K14me1 after infection with pathogenic bacteria. HIS-24K14me1 localization changes from being mostly nuclear to both nuclear and cytoplasmic in the intestinal cells of infected animals. Our results highlight an antimicrobial role of HIS-24K14me1 and suggest a functional link between epigenetic regulation by an HP1/H1 complex and the innate immune system in C. elegans."],["dc.identifier.doi","10.1128/MCB.05229-11"],["dc.identifier.gro","3142612"],["dc.identifier.isi","000299020100002"],["dc.identifier.pmid","22083954"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/35"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0270-7306"],["dc.title","Novel Roles of Caenorhabditis elegans Heterochromatin Protein HP1 and Linker Histone in the Regulation of Innate Immune Gene Expression"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","793"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","MHR Basic science of reproductive medicine"],["dc.bibliographiccitation.lastpage","803"],["dc.bibliographiccitation.volume","16"],["dc.contributor.author","Zovoilis, Athanasios"],["dc.contributor.author","Pantazi, Angeliki"],["dc.contributor.author","Smorag, Lukasz"],["dc.contributor.author","Opitz, Lennart"],["dc.contributor.author","Salinas Riester, Gabriela"],["dc.contributor.author","Wolf, Marieke"],["dc.contributor.author","Zechner, Ulrich"],["dc.contributor.author","Holubowska, Anna"],["dc.contributor.author","Stewart, Colin L."],["dc.contributor.author","Engel, Wolfgang"],["dc.date.accessioned","2018-11-07T08:37:42Z"],["dc.date.available","2018-11-07T08:37:42Z"],["dc.date.issued","2010"],["dc.description.abstract","Cells originating from the germ cell lineage retain the remarkable property under special culture conditions to give rise to cells with embryonic stem cell (ESC) properties, such as the multipotent adult germline stem cells (maGSCs) derived from adult mouse testis. To get an insight into the mechanisms that control pluripotency and differentiation in these cells, we studied how differences observed during in vitro differentiation between ESCs and maGSCs are associated with differences at the level of microRNAs (miRNAs). In this work, we provide for a first time a connection between germ cell origin of maGSCs and their specific miRNA expression profile. We found that maGSCs express higher levels of germ cell markers characteristic for primordial germ cells (PGCs) and spermatogonia compared with ESCs. Retained expression of miR-290 cluster has been previously reported in maGSCs during differentiation and it was associated with higher Oct-4 levels. Here, we show that this property is also shared by another pluripotent cell line originating from the germ line, the embryonic germ cells. In addition, we provide proof that the specific miRNA expression profile of maGSCs has an impact on their differentiation potential. Low levels of miR-302 in maGSCs during the first 10 days of leukaemia inhibitory factor deprivation are shown to be necessary for the maintenance of high levels of early germ cell markers."],["dc.identifier.doi","10.1093/molehr/gaq053"],["dc.identifier.isi","000283679900002"],["dc.identifier.pmid","20566704"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/18598"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.issn","1360-9947"],["dc.title","Embryonic stem cell-related miRNAs are involved in differentiation of pluripotent cells originating from the germ line"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","846"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","MHR Basic science of reproductive medicine"],["dc.bibliographiccitation.lastpage","855"],["dc.bibliographiccitation.volume","16"],["dc.contributor.author","Meyer, S."],["dc.contributor.author","Nolte, J."],["dc.contributor.author","Opitz, Lennart"],["dc.contributor.author","Salinas-Riester, Gabriela"],["dc.contributor.author","Engel, Wolfgang"],["dc.date.accessioned","2018-11-07T08:37:42Z"],["dc.date.available","2018-11-07T08:37:42Z"],["dc.date.issued","2010"],["dc.description.abstract","DNA microarray analysis was performed with mouse multipotent adult germline stem cells (maGSCs) and embryonic stem cells (ESCs) from different genetic backgrounds cultured under standard ESC-culture conditions and under differentiation-promoting conditions by the withdrawal of the leukemia inhibitory factor (LIF) and treatment with retinoic acid (RA). The analyzed undifferentiated cell lines are very similar based on their global gene expression pattern and show 97-99% identity dependent on the analyzed background. Only 621 genes are differentially expressed in cells derived from mouse 129SV-background and 72 genes show differences in expression in cells generated from transgenic Stra8-EGFP/Rosa26-LacZ-background. Both maGSCs and ESCs express the same genes involved in the regulation of pluripotency and even show no differences in the expression level of these genes. When comparing maGSCs with previously published signature genes of other pluripotent cell lines, we found that maGSCs shared a very similar gene expression pattern with embryonic germ cells (EGCs). Also after differentiation of maGSCs and ESCs the transcriptomes of the cell lines are nearly identical which suggests that both cell types differentiate spontaneously in a very similar way. This is the first study, at transcriptome level, to compare ESCs and a pluripotent cell line derived from an adult organism (maGSCs)."],["dc.description.sponsorship","German Research Foundation (DFG) [SPP1356, EN 84/22-1, 1041]"],["dc.identifier.doi","10.1093/molehr/gaq060"],["dc.identifier.isi","000283679900007"],["dc.identifier.pmid","20624824"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/18600"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press"],["dc.relation.issn","1360-9947"],["dc.title","Pluripotent embryonic stem cells and multipotent adult germline stem cells reveal similar transcriptomes including pluripotency-related genes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","413"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Zeitschrift für Gastroenterologie"],["dc.bibliographiccitation.lastpage","418"],["dc.bibliographiccitation.volume","41"],["dc.contributor.author","Opitz, T."],["dc.contributor.author","Buchwald, Arnd B."],["dc.contributor.author","Lorf, Thomas"],["dc.contributor.author","Awuah, David"],["dc.contributor.author","Ramadori, Giuliano"],["dc.contributor.author","Nolte, W."],["dc.date.accessioned","2018-11-07T10:39:30Z"],["dc.date.available","2018-11-07T10:39:30Z"],["dc.date.issued","2003"],["dc.description.abstract","We present a 40-year-old female patient with epigastric pain, ascites, and progressive liver failure, caused by Budd-Chiari syndrome (BCS) with thrombotic occlusion of the right and middle hepatic veins. As underlying diseases, essential thrombocythemia and resistance to activated protein C (APC) due to heterozygote factor V Leiden were found. Initial therapy with heparin caused thrombocytopenia (HIT) type 11 culminating in thrombosis of the last patent left hepatic vein and further deterioration of liver function. The decision against a surgical shunt and liver transplantation by our surgeons on the basis of the risks involved, prompted us to insert a transjugular intrahepatic portosystemic stent-shunt (TIPS). There was no measurable flow signal in the doppler sonography of the portal vein presumably due to thrombosis. A further evaluation with magnetic resonance tomography and angiography was impossible due to movement artefacts. TIPS initially served as a diagnostic tool allowing direct angiography-diagnosed thrombosis of the portal vein, the superior mesenteric and the splenic vein respectively. However, insertion of the TIPS shunt and subsequent fragmentation led to an effective hepatic decompression and full recanalisation of the portal vein. In the present case TIPS simultaneously allowed the diagnosis of portal vein thrombosis and served as rescue therapy of complicated Budd-Chiari syndrome. The potential development of HIT type II should be kept in mind when heparin is given, especially to patients with thrombophilia."],["dc.identifier.isi","000183193200007"],["dc.identifier.pmid","12772054"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/46063"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.issn","0044-2771"],["dc.title","The transjugular intrahepatic portosystemic stent-shunt (TIPS) as rescue therapy for complete Budd-Chiari syndrome and portal vein thrombosis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Prion"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Vanni, Silvia"],["dc.contributor.author","Barbisin, Maura"],["dc.contributor.author","Schmaedicke, Ann-Christin"],["dc.contributor.author","Motzkus, Dirk"],["dc.contributor.author","Opitz, Lennart"],["dc.contributor.author","Salinas-Riester, Gabriela"],["dc.contributor.author","Legname, Giuseppe"],["dc.date.accessioned","2018-11-07T09:41:29Z"],["dc.date.available","2018-11-07T09:41:29Z"],["dc.date.issued","2014"],["dc.format.extent","93"],["dc.identifier.isi","000340614900202"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/33744"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Landes Bioscience"],["dc.publisher.place","Austin"],["dc.relation.issn","1933-690X"],["dc.relation.issn","1933-6896"],["dc.title","Gene expression profiling of brains from bovine spongiform encephalopathy (BSE)-infected cynomolgus macaques"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","753"],["dc.bibliographiccitation.issue","5979"],["dc.bibliographiccitation.journal","Science"],["dc.bibliographiccitation.lastpage","756"],["dc.bibliographiccitation.volume","328"],["dc.contributor.author","Peleg, Shahaf"],["dc.contributor.author","Sananbenesi, Farahnaz"],["dc.contributor.author","Zovoilis, Athanasios"],["dc.contributor.author","Burkhardt, Susanne"],["dc.contributor.author","Bahari-Javan, Sanaz"],["dc.contributor.author","Agis-Balboa, Roberto Carlos"],["dc.contributor.author","Cota, Perla"],["dc.contributor.author","Wittnam, Jessica"],["dc.contributor.author","Opitz, Lennart"],["dc.contributor.author","Salinas-Riester, Gabriela"],["dc.contributor.author","Dettenhofer, Markus"],["dc.contributor.author","Kang, Hui"],["dc.contributor.author","Farinelli, Laurent"],["dc.contributor.author","Chen, Wei"],["dc.contributor.author","Fischer, Andre"],["dc.contributor.author","Doering, Aaron"],["dc.date.accessioned","2017-09-07T11:46:04Z"],["dc.date.available","2017-09-07T11:46:04Z"],["dc.date.issued","2010"],["dc.description.abstract","As the human life span increases, the number of people suffering from cognitive decline is rising dramatically. The mechanisms underlying age-associated memory impairment are, however, not understood. Here we show that memory disturbances in the aging brain of the mouse are associated with altered hippocampal chromatin plasticity. During learning, aged mice display a specific deregulation of histone H4 lysine 12 (H4K12) acetylation and fail to initiate a hippocampal gene expression program associated with memory consolidation. Restoration of physiological H4K12 acetylation reinstates the expression of learning-induced genes and leads to the recovery of cognitive abilities. Our data suggest that deregulated H4K12 acetylation may represent an early biomarker of an impaired genome-environment interaction in the aging mouse brain."],["dc.identifier.doi","10.1126/science.1186088"],["dc.identifier.gro","3142928"],["dc.identifier.isi","000277357100040"],["dc.identifier.pmid","20448184"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/386"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0036-8075"],["dc.title","Altered Histone Acetylation Is Associated with Age-Dependent Memory Impairment in Mice"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Cardiovascular Research"],["dc.bibliographiccitation.volume","87"],["dc.contributor.author","Matthes, F."],["dc.contributor.author","Steinbrecher, Julia H."],["dc.contributor.author","Salinas-Riester, Gabriela"],["dc.contributor.author","Opitz, Lennart"],["dc.contributor.author","Hasenfuß, Gerd"],["dc.contributor.author","Lehnart, Stephan E."],["dc.date.accessioned","2018-11-07T08:41:33Z"],["dc.date.available","2018-11-07T08:41:33Z"],["dc.date.issued","2010"],["dc.format.extent","S65"],["dc.identifier.isi","000282114100100"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/19495"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press"],["dc.publisher.place","Oxford"],["dc.relation.eventlocation","Berlin, GERMANY"],["dc.relation.issn","0008-6363"],["dc.title","Molecular genomics of cardiac remodeling following changes in intracellular calcium release"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","434"],["dc.bibliographiccitation.journal","BMC Genomics"],["dc.bibliographiccitation.volume","15"],["dc.contributor.author","Barbisin, Maura"],["dc.contributor.author","Vanni, Silvia"],["dc.contributor.author","Schmaedicke, Ann-Christin"],["dc.contributor.author","Montag, Judith"],["dc.contributor.author","Motzkus, Dirk"],["dc.contributor.author","Opitz, Lennart"],["dc.contributor.author","Salinas-Riester, Gabriela"],["dc.contributor.author","Legname, Giuseppe"],["dc.date.accessioned","2018-11-07T09:38:59Z"],["dc.date.available","2018-11-07T09:38:59Z"],["dc.date.issued","2014"],["dc.description.abstract","Background: Prion diseases are fatal neurodegenerative disorders whose pathogenesis mechanisms are not fully understood. In this context, the analysis of gene expression alterations occurring in prion-infected animals represents a powerful tool that may contribute to unravel the molecular basis of prion diseases and therefore discover novel potential targets for diagnosis and therapeutics. Here we present the first large-scale transcriptional profiling of brains from BSE-infected cynomolgus macaques, which are an excellent model for human prion disorders. Results: The study was conducted using the GeneChip (R) Rhesus Macaque Genome Array and revealed 300 transcripts with expression changes greater than twofold. Among these, the bioinformatics analysis identified 86 genes with known functions, most of which are involved in cellular development, cell death and survival, lipid homeostasis, and acute phase response signaling. RT-qPCR was performed on selected gene transcripts in order to validate the differential expression in infected animals versus controls. The results obtained with the microarray technology were confirmed and a gene signature was identified. In brief, HBB and HBA2 were down-regulated in infected macaques, whereas TTR, APOC1 and SERPINA3 were up-regulated. Conclusions: Some genes involved in oxygen or lipid transport and in innate immunity were found to be dysregulated in prion infected macaques. These genes are known to be involved in other neurodegenerative disorders such as Alzheimer's and Parkinson's diseases. Our results may facilitate the identification of potential disease biomarkers for many neurodegenerative diseases."],["dc.identifier.doi","10.1186/1471-2164-15-434"],["dc.identifier.isi","000338257000001"],["dc.identifier.pmid","24898206"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/33181"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1471-2164"],["dc.title","Gene expression profiling of brains from bovine spongiform encephalopathy (BSE)-infected cynomolgus macaques"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]