Danzl, Johann Georg

[["dc.bibliographiccitation.firstpage","3290"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Angewandte Chemie International Edition"],["dc.bibliographiccitation.lastpage","3294"],["dc.bibliographiccitation.volume","55"],["dc.contributor.author","Butkevich, Alexey N."],["dc.contributor.author","Mitronova, Gyuzel Yu"],["dc.contributor.author","Sidenstein, Sven C."],["dc.contributor.author","Klocke, Jessica L."],["dc.contributor.author","Kamin, Dirk"],["dc.contributor.author","Meineke, Dirk N. H."],["dc.contributor.author","D'Este, E."],["dc.contributor.author","Kraemer, Philip-Tobias"],["dc.contributor.author","Danzl, Johann G."],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:54:38Z"],["dc.date.available","2017-09-07T11:54:38Z"],["dc.date.issued","2016"],["dc.description.abstract","A range of bright and photostable rhodamines and carbopyronines with absorption maxima in the range of =500-630nm were prepared, and enabled the specific labeling of cytoskeletal filaments using HaloTag technology followed by staining with 1m solutions of the dye-ligand conjugates. The synthesis, photophysical parameters, fluorogenic behavior, and structure-property relationships of the new dyes are discussed. Light microscopy with stimulated emission depletion (STED) provided one- and two-color images of living cells with an optical resolution of 40-60nm."],["dc.identifier.doi","10.1002/anie.201511018"],["dc.identifier.gro","3141724"],["dc.identifier.isi","000371418200008"],["dc.identifier.pmid","26844929"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/369"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1521-3773"],["dc.relation.issn","1433-7851"],["dc.title","Fluorescent Rhodamines and Fluorogenic Carbopyronines for Super-Resolution STED Microscopy in Living Cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Gregor, Carola"],["dc.contributor.author","Sidenstein, Sven C."],["dc.contributor.author","Andresen, Martin"],["dc.contributor.author","Sahl, Steffen J."],["dc.contributor.author","Danzl, Johann G."],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2018-04-23T11:48:22Z"],["dc.date.available","2018-04-23T11:48:22Z"],["dc.date.issued","2018"],["dc.description.abstract","The reversibly switchable fluorescent proteins (RSFPs) commonly used for RESOLFT nanoscopy have been developed from fluorescent proteins of the GFP superfamily. These proteins are bright, but exhibit several drawbacks such as relatively large size, oxygen-dependence, sensitivity to low pH, and limited switching speed. Therefore, RSFPs from other origins with improved properties need to be explored. Here, we report the development of two RSFPs based on the LOV domain of the photoreceptor protein YtvA from Bacillus subtilis. LOV domains obtain their fluorescence by association with the abundant cellular cofactor flavin mononucleotide (FMN). Under illumination with blue and ultraviolet light, they undergo a photocycle, making these proteins inherently photoswitchable. Our first improved variant, rsLOV1, can be used for RESOLFT imaging, whereas rsLOV2 proved useful for STED nanoscopy of living cells with a resolution of down to 50 nm. In addition to their smaller size compared to GFP-related proteins (17 kDa instead of 27 kDa) and their usability at low pH, rsLOV1 and rsLOV2 exhibit faster switching kinetics, switching on and off 3 times faster than rsEGFP2, the fastest-switching RSFP reported to date. Therefore, LOV-domain-based RSFPs have potential for applications where the switching speed of GFP-based proteins is limiting."],["dc.identifier.doi","10.1038/s41598-018-19947-1"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/13496"],["dc.language.iso","en"],["dc.notes.status","zu prüfen"],["dc.relation.issn","2045-2322"],["dc.title","Novel reversibly switchable fluorescent proteins for RESOLFT and STED nanoscopy engineered from the bacterial photoreceptor YtvA"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","756"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","ChemPhysChem"],["dc.bibliographiccitation.lastpage","762"],["dc.bibliographiccitation.volume","15"],["dc.contributor.author","Jensen, Nickels A."],["dc.contributor.author","Danzl, Johann G."],["dc.contributor.author","Willig, Katrin I."],["dc.contributor.author","Lavoie-Cardinal, Flavie"],["dc.contributor.author","Brakemann, Tanja"],["dc.contributor.author","Hell, Stefan W."],["dc.contributor.author","Jakobs, Stefan"],["dc.date.accessioned","2017-09-07T11:46:25Z"],["dc.date.available","2017-09-07T11:46:25Z"],["dc.date.issued","2014"],["dc.description.abstract","Diffraction-unlimited far-field super-resolution fluorescence (nanoscopy) methods typically rely on transiently transferring fluorophores between two states, whereby this transfer is usually laid out as a switch. However, depending on whether this is induced in a spatially controlled manner using a pattern of light (coordinate-targeted) or stochastically on a single-molecule basis, specific requirements on the fluorophores are imposed. Therefore, the fluorophores are usually utilized just for one class of methods only. In this study we demonstrate that the reversibly switchable fluorescent protein Dreiklang enables live-cell recordings in both spatially controlled and stochastic modes. We show that the Dreiklang chromophore entails three different light-induced switching mechanisms, namely a reversible photochemical one, off-switching by stimulated emission, and a reversible transfer to a long-lived dark state from the S-1 state, all of which can be utilized to overcome the diffraction barrier. We also find that for the single-molecule-based stochastic GSDIM approach (ground-state depletion followed by individual molecule return), Dreiklang provides a larger number of on-off localization events as compared to its progenitor Citrine. Altogether, Dreiklang is a versatile probe for essentially all popular forms of live-cell fluorescence nanoscopy."],["dc.identifier.doi","10.1002/cphc.201301034"],["dc.identifier.gro","3142169"],["dc.identifier.isi","000332747500026"],["dc.identifier.pmid","24497300"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/5299"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1439-7641"],["dc.relation.issn","1439-4235"],["dc.title","Coordinate-Targeted and Coordinate-Stochastic Super-Resolution Microscopy with the Reversibly Switchable Fluorescent Protein Dreiklang"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","832"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Nature Protocols"],["dc.bibliographiccitation.lastpage","863"],["dc.bibliographiccitation.volume","14"],["dc.contributor.author","Truckenbrodt, Sven"],["dc.contributor.author","Sommer, Christoph"],["dc.contributor.author","Rizzoli, Silvio O."],["dc.contributor.author","Danzl, Johann G."],["dc.date.accessioned","2019-07-09T11:50:31Z"],["dc.date.available","2019-07-09T11:50:31Z"],["dc.date.issued","2019"],["dc.description.abstract","Expansion microscopy is a relatively new approach to super-resolution imaging that uses expandable hydrogels to isotropically increase the physical distance between fluorophores in biological samples such as cell cultures or tissue slices. The classic gel recipe results in an expansion factor of ~4×, with a resolution of 60-80 nm. We have recently developed X10 microscopy, which uses a gel that achieves an expansion factor of ~10×, with a resolution of ~25 nm. Here, we provide a step-by-step protocol for X10 expansion microscopy. A typical experiment consists of seven sequential stages: (i) immunostaining, (ii) anchoring, (iii) polymerization, (iv) homogenization, (v) expansion, (vi) imaging, and (vii) validation. The protocol presented here includes recommendations for optimization, pitfalls and their solutions, and detailed guidelines that should increase reproducibility. Although our protocol focuses on X10 expansion microscopy, we detail which of these suggestions are also applicable to classic fourfold expansion microscopy. We exemplify our protocol using primary hippocampal neurons from rats, but our approach can be used with other primary cells or cultured cell lines of interest. This protocol will enable any researcher with basic experience in immunostainings and access to an epifluorescence microscope to perform super-resolution microscopy with X10. The procedure takes 3 d and requires ~5 h of actively handling the sample for labeling and expansion, and another ~3 h for imaging and analysis."],["dc.identifier.doi","10.1038/s41596-018-0117-3"],["dc.identifier.pmid","30778205"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15953"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59786"],["dc.identifier.url","https://sfb1286.uni-goettingen.de/literature/publications/3"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation","info:eu-repo/grantAgreement/EC/FP7/614765/EU//NEUROMOLANATOMY"],["dc.relation","SFB 1286: Quantitative Synaptologie"],["dc.relation","SFB 1286 | Z03: Unkomplizierte multispektrale, superauflösende Bildgebung durch zehnfache Expansionsmikroskopie"],["dc.relation.issn","1750-2799"],["dc.relation.workinggroup","RG Rizzoli (Quantitative Synaptology in Space and Time)"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","610"],["dc.title","A practical guide to optimization in X10 expansion microscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","122"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Nature Photonics"],["dc.bibliographiccitation.lastpage","128"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Danzl, Johann G."],["dc.contributor.author","Sidenstein, Sven C."],["dc.contributor.author","Gregor, Carola"],["dc.contributor.author","Urban, Nicolai T."],["dc.contributor.author","Ilgen, Peter"],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2017-09-07T11:54:41Z"],["dc.date.available","2017-09-07T11:54:41Z"],["dc.date.issued","2016"],["dc.description.abstract","Far-field super-resolution fluorescence microscopy discerns fluorophores residing closer than the diffraction barrier by briefly transferring them in different (typically ON and OFF) states before detection. In coordinate-targeted super-resolution variants, such as stimulated emission depletion (STED) microscopy, this state difference is created by the intensity minima and maxima of an optical pattern, causing all fluorophores to assume the off state, for instance, except at the minima. Although strong spatial confinement of the on state enables high resolution, it also subjects the fluorophores to excess intensities and state cycles at the maxima. Here, we address these issues by driving the fluorophores into a second off state that is inert to the excess light. By using reversibly switchable fluorescent proteins as labels, our approach reduces bleaching and enhances resolution and contrast in live-cell STED microscopy. Using two or more transitions to off states is a useful strategy for augmenting the power of coordinate-targeted super-resolution microscopy."],["dc.identifier.doi","10.1038/NPHOTON.2015.266"],["dc.identifier.gro","3141737"],["dc.identifier.isi","000369321400015"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/513"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: European Union [PIEF-GA-2011-299283]; Korber Foundation"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1749-4893"],["dc.relation.issn","1749-4885"],["dc.title","Coordinate-targeted fluorescence nanoscopy with multiple off states"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","186a"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","187a"],["dc.bibliographiccitation.volume","112"],["dc.contributor.author","Danzl, Johann G."],["dc.contributor.author","Sidenstein, Sven"],["dc.contributor.author","Gregor, Carola"],["dc.contributor.author","Urban, Nicolai"],["dc.contributor.author","Ilgen, Peter"],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2022-03-01T11:44:58Z"],["dc.date.available","2022-03-01T11:44:58Z"],["dc.date.issued","2017"],["dc.identifier.doi","10.1016/j.bpj.2016.11.1034"],["dc.identifier.pii","S0006349516320641"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103178"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-531"],["dc.relation.issn","0006-3495"],["dc.title","Coordinate-Targeted Fluorescence Nanoscopy with Multiple Off-States"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","26725"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","6"],["dc.contributor.author","Sidenstein, Sven C."],["dc.contributor.author","D'Este, Elisa"],["dc.contributor.author","Böhm, Marvin J."],["dc.contributor.author","Danzl, Johann G."],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:44:54Z"],["dc.date.available","2017-09-07T11:44:54Z"],["dc.date.issued","2016"],["dc.description.abstract","Superresolution fluorescence microscopy of multiple fluorophores still requires development. Here we present simultaneous three-colour stimulated emission depletion (STED) nanoscopy relying on a single STED beam at 620 nm. Toggling the STED beam between two or more power levels (\"multilevelSTED\") optimizes resolution and contrast in all colour channels, which are intrinsically co-aligned and well separated. Three-colour recording is demonstrated by imaging the nanoscale cytoskeletal organization in cultured hippocampal neurons. The down to similar to 35 nm resolution identified periodic actin/betaII spectrin lattices along dendrites and spines; however, at presynaptic and postsynaptic sites, these patterns were found to be absent. Both our multicolour scheme and the 620 nm STED line should be attractive for routine STED microscopy applications."],["dc.identifier.doi","10.1038/srep26725"],["dc.identifier.gro","3141682"],["dc.identifier.isi","000376500200002"],["dc.identifier.pmid","27220554"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13375"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/8850"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","2045-2322"],["dc.rights.access","openAccess"],["dc.title","Multicolour Multilevel STED nanoscopy of Actin/Spectrin Organization at Synapses"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]