Keppeler, Daniel

[["dc.bibliographiccitation.firstpage","35"],["dc.bibliographiccitation.journal","Acta Physiologica"],["dc.bibliographiccitation.lastpage","36"],["dc.bibliographiccitation.volume","213"],["dc.contributor.author","Keppeler, Daniel"],["dc.contributor.author","Jeschke, Marcus"],["dc.contributor.author","Wrobel, C."],["dc.contributor.author","Hoch, Gerhard"],["dc.contributor.author","Gossler, Christian"],["dc.contributor.author","Schwarz, U. T."],["dc.contributor.author","Ruther, P."],["dc.contributor.author","Schwaerzle, M."],["dc.contributor.author","Hessler, R."],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","Kügler, Sebastian"],["dc.contributor.author","Moser, Tobias"],["dc.date.accessioned","2018-11-07T09:59:50Z"],["dc.date.available","2018-11-07T09:59:50Z"],["dc.date.issued","2015"],["dc.identifier.isi","000362554200073"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/37681"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.publisher.place","Hoboken"],["dc.title","In vivo application of optogenetics in the auditory system"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","eabb8086"],["dc.bibliographiccitation.issue","553"],["dc.bibliographiccitation.journal","Science Translational Medicine"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Keppeler, Daniel"],["dc.contributor.author","Schwaerzle, Michael"],["dc.contributor.author","Harczos, Tamas"],["dc.contributor.author","Jablonski, Lukasz"],["dc.contributor.author","Dieter, Alexander"],["dc.contributor.author","Wolf, Bettina"],["dc.contributor.author","Ayub, Suleman"],["dc.contributor.author","Vogl, Christian"],["dc.contributor.author","Wrobel, Christian"],["dc.contributor.author","Hoch, Gerhard"],["dc.contributor.author","Abdellatif, Khaled"],["dc.contributor.author","Jeschke, Marcus"],["dc.contributor.author","Rankovic, Vladan"],["dc.contributor.author","Paul, Oliver"],["dc.contributor.author","Ruther, Patrick"],["dc.contributor.author","Moser, Tobias"],["dc.date.accessioned","2021-03-17T06:28:44Z"],["dc.date.available","2021-03-17T06:28:44Z"],["dc.date.issued","2020-07-22"],["dc.description.abstract","When hearing fails, electrical cochlear implants (eCIs) provide the brain with auditory information. One important bottleneck of CIs is the poor spectral selectivity that results from the wide current spread from each of the electrode contacts. Optical CIs (oCIs) promise to make better use of the tonotopic order of spiral ganglion neurons (SGNs) inside the cochlea by spatially confined stimulation. Here, we established multichannel oCIs based on light-emitting diode (LED) arrays and used them for optical stimulation of channelrhodopsin (ChR)-expressing SGNs in rodents. Power-efficient blue LED chips were integrated onto microfabricated 15-μm-thin polyimide-based carriers comprising interconnecting lines to address individual LEDs by a stationary or mobile driver circuitry. We extensively characterized the optoelectronic, thermal, and mechanical properties of the oCIs and demonstrated stability over weeks in vitro. We then implanted the oCIs into ChR-expressing rats and gerbils, and characterized multichannel optogenetic SGN stimulation by electrophysiological and behavioral experiments. Improved spectral selectivity was directly demonstrated by recordings from the auditory midbrain. Long-term experiments in deafened ChR-expressing rats and in nontreated control animals demonstrated specificity of optogenetic stimulation. Behavioral studies on animals carrying a wireless oCI sound processor revealed auditory percepts. This study demonstrates hearing restoration with improved spectral selectivity by an LED-based multichannel oCI system."],["dc.identifier.doi","10.1126/scitranslmed.abb8086"],["dc.identifier.pmid","32718992"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/80539"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/57"],["dc.language.iso","en"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","1946-6242"],["dc.relation.issn","1946-6234"],["dc.relation.orgunit","Institut für Auditorische Neurowissenschaften"],["dc.relation.workinggroup","RG Moser (Molecular Anatomy, Physiology and Pathology of Sound Encoding)"],["dc.title","Multichannel optogenetic stimulation of the auditory pathway using microfabricated LED cochlear implants in rodents"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","HNO"],["dc.contributor.author","Wrobel, Christian"],["dc.contributor.author","Keppeler, Daniel"],["dc.contributor.author","Meyer, Alexander C."],["dc.date.accessioned","2021-09-01T06:42:47Z"],["dc.date.available","2021-09-01T06:42:47Z"],["dc.date.issued","2021"],["dc.description.abstract","Zusammenfassung Hintergrund Plattenbasierte Ankersysteme zur fazialen Epithesenversorgung bieten gegenüber extraoralen Einzeltitanimplantaten Vorteile hinsichtlich einer flexibleren Wahl knöcherner Verankerungspunkte und höherer Stabilität. Nachteile werden in einer aufwendigen individuellen intraoperativen Anpassung der Plattensysteme am meist schlecht zugänglichen Knochen deutlich. Wir stellen eine Methode vor, diese Nachteile zu überwinden und die Vorteile plattenbasierter Systeme stärker auszuspielen. Methodik Das knöcherne Mittegesicht eines Patienten mit erfolgter Rhinektomie bei Karzinom des Naseneingangs wurde anhand der präoperativen Computertomographie als virtuelles 3‑D-Modell rekonstruiert. Die verwendete Open-Source-Software (3-D-Sclicer) ermöglichte die einfache und schnelle Rekonstruktion sowie Anpassung zum Druck des 3‑D-Modells mittels transparenten Kunststoffs (MED610; stratasys Ltd., MN, USA). Ergebnisse Die als Epithesenanker verwendete Titan-Brückenplatte (MEDICON) konnte am 3‑D-Druck des Mittelgesichts äußerst präzise vorangepasst werden. Wichtige anatomische Strukturen wurden geschont und die Verschraubungspunkte entsprechend der gegebenen Knochendicke gewählt. Die Implantation der vorangepassten Titanplatte erfolgte komplikationslos ohne weitere intraoperative Anpassungen. Schlussfolgerung Die Voranpassung plattenbasierter Ankersysteme für faziale Epithesen am 3‑D-Druck des Mittelgesichts überwindet deren Nachteile einer aufwendigen ggf. unpräzisen intraoperativen individuellen Anpassung. Diese Methode spielt die Vorteile der besseren Kraftverteilung durch mehr mögliche Verschraubungen, auch in dünnerem Knochen, weiter aus und kann somit Implantatlockerungen vorbeugen. Zudem ermöglicht die Voranpassung am 3‑D-Modell die bessere Identifikation und Schonung wichtiger anatomischer Strukturen und spart Op.-Zeit ein."],["dc.description.abstract","Abstract Background Plate-based anchorage systems for craniofacial prostheses offer advantages over extraoral solitary titanium implants in terms of the flexible choice of mounting points and higher stability. Disadvantages become apparent in the complex individual intraoperative adaptation of the plate-based systems to the usually poorly accessible bone. The current article presents a method to overcome these disadvantages and make greater use of the advantages of plate-based systems. Materials and methods The bony midface of a patient who had undergone rhinectomy for cancer of the nasal entrance was reconstructed as a virtual 3D model based on preoperative CT. The open-source software (3D-Slicer) allowed easy and fast reconstruction as well as adaptation for 3D printing using transparent plastic (MED610; stratasys Ltd., MN, USA). Results A titanium mini-plate (MEDICON) for anchoring the nasal prosthesis could be fitted extremely precisely on the midface 3D print. Important anatomical structures were spared, and screw placement was selected according to the individual bone thickness. Implantation of the in-advance fitted titanium plate was performed without complications and without further adjustments. Conclusion In-advance fitting of plate-based systems for anchorage of craniofacial prostheses using 3D printing of the midface overcomes their disadvantages of time-consuming and possibly imprecise individual adaptation. This method further exploits the advantages of higher stability through more possible mounting points, even in thinner bone, to prevent loosening. In addition, in-advance fitting of titanium plates on the 3D model enables better identification and protection of important anatomical structures and shortens operative time."],["dc.description.abstract","Zusammenfassung Hintergrund Plattenbasierte Ankersysteme zur fazialen Epithesenversorgung bieten gegenüber extraoralen Einzeltitanimplantaten Vorteile hinsichtlich einer flexibleren Wahl knöcherner Verankerungspunkte und höherer Stabilität. Nachteile werden in einer aufwendigen individuellen intraoperativen Anpassung der Plattensysteme am meist schlecht zugänglichen Knochen deutlich. Wir stellen eine Methode vor, diese Nachteile zu überwinden und die Vorteile plattenbasierter Systeme stärker auszuspielen. Methodik Das knöcherne Mittegesicht eines Patienten mit erfolgter Rhinektomie bei Karzinom des Naseneingangs wurde anhand der präoperativen Computertomographie als virtuelles 3‑D-Modell rekonstruiert. Die verwendete Open-Source-Software (3-D-Sclicer) ermöglichte die einfache und schnelle Rekonstruktion sowie Anpassung zum Druck des 3‑D-Modells mittels transparenten Kunststoffs (MED610; stratasys Ltd., MN, USA). Ergebnisse Die als Epithesenanker verwendete Titan-Brückenplatte (MEDICON) konnte am 3‑D-Druck des Mittelgesichts äußerst präzise vorangepasst werden. Wichtige anatomische Strukturen wurden geschont und die Verschraubungspunkte entsprechend der gegebenen Knochendicke gewählt. Die Implantation der vorangepassten Titanplatte erfolgte komplikationslos ohne weitere intraoperative Anpassungen. Schlussfolgerung Die Voranpassung plattenbasierter Ankersysteme für faziale Epithesen am 3‑D-Druck des Mittelgesichts überwindet deren Nachteile einer aufwendigen ggf. unpräzisen intraoperativen individuellen Anpassung. Diese Methode spielt die Vorteile der besseren Kraftverteilung durch mehr mögliche Verschraubungen, auch in dünnerem Knochen, weiter aus und kann somit Implantatlockerungen vorbeugen. Zudem ermöglicht die Voranpassung am 3‑D-Modell die bessere Identifikation und Schonung wichtiger anatomischer Strukturen und spart Op.-Zeit ein."],["dc.description.abstract","Abstract Background Plate-based anchorage systems for craniofacial prostheses offer advantages over extraoral solitary titanium implants in terms of the flexible choice of mounting points and higher stability. Disadvantages become apparent in the complex individual intraoperative adaptation of the plate-based systems to the usually poorly accessible bone. The current article presents a method to overcome these disadvantages and make greater use of the advantages of plate-based systems. Materials and methods The bony midface of a patient who had undergone rhinectomy for cancer of the nasal entrance was reconstructed as a virtual 3D model based on preoperative CT. The open-source software (3D-Slicer) allowed easy and fast reconstruction as well as adaptation for 3D printing using transparent plastic (MED610; stratasys Ltd., MN, USA). Results A titanium mini-plate (MEDICON) for anchoring the nasal prosthesis could be fitted extremely precisely on the midface 3D print. Important anatomical structures were spared, and screw placement was selected according to the individual bone thickness. Implantation of the in-advance fitted titanium plate was performed without complications and without further adjustments. Conclusion In-advance fitting of plate-based systems for anchorage of craniofacial prostheses using 3D printing of the midface overcomes their disadvantages of time-consuming and possibly imprecise individual adaptation. This method further exploits the advantages of higher stability through more possible mounting points, even in thinner bone, to prevent loosening. In addition, in-advance fitting of titanium plates on the 3D model enables better identification and protection of important anatomical structures and shortens operative time."],["dc.identifier.doi","10.1007/s00106-021-01100-6"],["dc.identifier.pii","1100"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/89144"],["dc.language.iso","de"],["dc.notes.intern","DOI-Import GROB-455"],["dc.relation.eissn","1433-0458"],["dc.relation.issn","0017-6192"],["dc.title","3-D-Druck-optimierte Anpassung eines Mittelgesichtsimplantats zur magnetgetragenen nasalen Epithesenversorgung"],["dc.title.translated","Optimized fitting of a midface implant to anchor a magnetic nasal prosthesis using 3D printing"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","205401"],["dc.bibliographiccitation.issue","20"],["dc.bibliographiccitation.journal","Journal of Physics D: Applied Physics"],["dc.bibliographiccitation.volume","47"],["dc.contributor.author","Gossler, Christian"],["dc.contributor.author","Bierbrauer, Colin"],["dc.contributor.author","Moser, Ruediger"],["dc.contributor.author","Kunzer, Michael"],["dc.contributor.author","Holc, Katarzyna"],["dc.contributor.author","Pletschen, Wilfried"],["dc.contributor.author","Koehler, Klaus"],["dc.contributor.author","Wagner, Joachim"],["dc.contributor.author","Schwaerzle, Michael"],["dc.contributor.author","Ruther, Patrick"],["dc.contributor.author","Paul, Oliver"],["dc.contributor.author","Neef, Jakob"],["dc.contributor.author","Keppeler, Daniel"],["dc.contributor.author","Hoch, Gerhard"],["dc.contributor.author","Moser, Tobias"],["dc.contributor.author","Schwarz, Ulrich T."],["dc.date.accessioned","2017-09-07T11:46:14Z"],["dc.date.available","2017-09-07T11:46:14Z"],["dc.date.issued","2014"],["dc.description.abstract","Currently available cochlear implants are based on electrical stimulation of the spiral ganglion neurons. Optical stimulation with arrays of micro-sized light-emitting diodes (mu LEDs) promises to increase the number of distinguishable frequencies. Here, the development of a flexible GaN-based micro-LED array as an optical cochlear implant is reported for application in a mouse model. The fabrication of 15 mu m thin and highly flexible devices is enabled by a laser-based layer transfer process of the GaN-LEDs from sapphire to a polyimide-on-silicon carrier wafer. The fabricated 50 x 50 mu m(2) LEDs are contacted via conducting paths on both p- and n-sides of the LEDs. Up to three separate channels could be addressed. The probes, composed of a linear array of the said mu LEDs bonded to the flexible polyimide substrate, are peeled off the carrier wafer and attached to flexible printed circuit boards. Probes with four mu LEDs and a width of 230 mu m are successfully implanted in the mouse cochlea both in vitro and in vivo. The LEDs emit 60 mu W at 1 mA after peel-off, corresponding to a radiant emittance of 6 mW mm(-2)."],["dc.identifier.doi","10.1088/0022-3727/47/20/205401"],["dc.identifier.gro","3142121"],["dc.identifier.isi","000335517500011"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/4777"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Iop Publishing Ltd"],["dc.relation.eissn","1361-6463"],["dc.relation.issn","0022-3727"],["dc.title","GaN-based micro-LED arrays on flexible substrates for optical cochlear implants"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","European Biophysics Journal"],["dc.bibliographiccitation.volume","44"],["dc.contributor.author","Keppeler, Daniel"],["dc.contributor.author","Hernandez, Victor H."],["dc.contributor.author","Gehrt, Anna"],["dc.contributor.author","Jeschke, Marcus"],["dc.contributor.author","Wrobel, C."],["dc.contributor.author","Hoch, Gerhard"],["dc.contributor.author","Gossler, C."],["dc.contributor.author","Schwaerzle, M."],["dc.contributor.author","Strenzke, Nicola"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","Kügler, Sebastian"],["dc.contributor.author","Ruther, P."],["dc.contributor.author","Schwarz, U. T."],["dc.contributor.author","Moser, Tobias"],["dc.date.accessioned","2018-11-07T09:55:33Z"],["dc.date.available","2018-11-07T09:55:33Z"],["dc.date.issued","2015"],["dc.identifier.isi","000380001400775"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/36770"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.publisher.place","New york"],["dc.relation.eventlocation","Dresden, GERMANY"],["dc.title","Employing optogenetics for auditory neuroscience and cochlear implants"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","eaao0540"],["dc.bibliographiccitation.issue","449"],["dc.bibliographiccitation.journal","Science Translational Medicine"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Wrobel, Christian"],["dc.contributor.author","Dieter, Alexander"],["dc.contributor.author","Huet, Antoine"],["dc.contributor.author","Keppeler, Daniel"],["dc.contributor.author","Duque-Afonso, Carlos J."],["dc.contributor.author","Vogl, Christian"],["dc.contributor.author","Hoch, Gerhard"],["dc.contributor.author","Jeschke, Marcus"],["dc.contributor.author","Moser, Tobias"],["dc.date.accessioned","2020-12-10T18:36:47Z"],["dc.date.available","2020-12-10T18:36:47Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1126/scitranslmed.aao0540"],["dc.identifier.eissn","1946-6242"],["dc.identifier.issn","1946-6234"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/76736"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Optogenetic stimulation of cochlear neurons activates the auditory pathway and restores auditory-driven behavior in deaf adult gerbils"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]