Napp, Joanna

[["dc.bibliographiccitation.firstpage","6367"],["dc.bibliographiccitation.issue","22"],["dc.bibliographiccitation.journal","Theranostics"],["dc.bibliographiccitation.lastpage","6383"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Napp, Joanna"],["dc.contributor.author","Markus, M. Andrea"],["dc.contributor.author","Heck, Joachim G."],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","Möbius, Wiebke"],["dc.contributor.author","Gorpas, Dimitris"],["dc.contributor.author","Feldmann, Claus"],["dc.contributor.author","Alves, Frauke"],["dc.date.accessioned","2019-07-09T11:49:44Z"],["dc.date.available","2019-07-09T11:49:44Z"],["dc.date.issued","2018"],["dc.description.abstract","Treatment of inflammatory disorders with glucocorticoids (GCs) is often accompanied by severe adverse effects. Application of GCs via nanoparticles (NPs), especially those using simple formulations, could possibly improve their delivery to sites of inflammation and therefore their efficacy, minimising the required dose and thus reducing side effects. Here, we present the evaluation of NPs composed of GC betamethasone phosphate (BMP) and the fluorescent dye DY-647 (BMP-IOH-NPs) for improved treatment of inflammation with simultaneous in vivo monitoring of NP delivery. Methods: BMP-IOH-NP uptake by MH-S macrophages was analysed by fluorescence and electron microscopy. Lipopolysaccharide (LPS)-stimulated cells were treated for 48 h with BMP-IOH-NPs (1×10-5-1×10-9 M), BMP or dexamethasone (Dexa). Drug efficacy was assessed by measurement of interleukin 6. Mice with Zymosan-A-induced paw inflammation were intraperitoneally treated with BMP-IOH-NPs (10 mg/kg) and mice with ovalbumin (OVA)-induced allergic airway inflammation (AAI) were treated intranasally with BMP-IOH-NPs, BMP or Dexa (each 2.5 mg/kg). Efficacy was assessed in vivo by paw volume measurements with µCT and ex vivo by measurement of paw weight for Zymosan-A-treated mice, or in the AAI model by in vivo x-ray-based lung function assessment and by cell counts in the bronchoalveolar lavage (BAL) fluid and histology. Delivery of BMP-IOH-NPs to the lungs of AAI mice was monitored by in vivo optical imaging and by fluorescence microscopy. Results: Uptake of BMP-IOH-NPs by MH-S cells was observed during the first 10 min of incubation, with the NP load increasing over time. The anti-inflammatory effect of BMP-IOH-NPs in vitro was dose dependent and higher than that of Dexa or free BMP, confirming efficient release of the drug. In vivo, Zymosan-A-induced paw inflammation was significantly reduced in mice treated with BMP-IOH-NPs. AAI mice that received BMP-IOH-NPs or Dexa but not BMP revealed significantly decreased eosinophil numbers in BALs and reduced immune cell infiltration in lungs. Correspondingly, lung function parameters, which were strongly affected in non-treated AAI mice, were unaffected in AAI mice treated with BMP-IOH-NPs and resembled those of healthy animals. Accumulation of BMP-IOH-NPs within the lungs of AAI mice was detectable by optical imaging for at least 4 h in vivo, where they were preferentially taken up by peribronchial and alveolar M2 macrophages. Conclusion: Our results show that BMP-IOH-NPs can effectively be applied in therapy of inflammatory diseases with at least equal efficacy as the gold standard Dexa, while their delivery can be simultaneously tracked in vivo by fluorescence imaging. BMP-IOH-NPs thus have the potential to reach clinical applications."],["dc.identifier.doi","10.7150/thno.28324"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15757"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59620"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1838-7640"],["dc.rights","CC BY-NC 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc/4.0"],["dc.subject.ddc","610"],["dc.title","Therapeutic Fluorescent Hybrid Nanoparticles for Traceable Delivery of Glucocorticoids to Inflammatory Sites"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","24"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","ChemNanoMat"],["dc.bibliographiccitation.lastpage","45"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Neumeier, B. Lilli"],["dc.contributor.author","Khorenko, Mikhail"],["dc.contributor.author","Alves, Frauke"],["dc.contributor.author","Goldmann, Oliver"],["dc.contributor.author","Napp, Joanna"],["dc.contributor.author","Schepers, Ute"],["dc.contributor.author","Reichardt, Holger M."],["dc.contributor.author","Feldmann, Claus"],["dc.date.accessioned","2022-03-01T11:45:18Z"],["dc.date.available","2022-03-01T11:45:18Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1002/cnma.201800310"],["dc.identifier.issn","2199-692X"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103282"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-531"],["dc.relation.issn","2199-692X"],["dc.title","Fluorescent Inorganic-Organic Hybrid Nanoparticles"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3860"],["dc.bibliographiccitation.issue","16"],["dc.bibliographiccitation.journal","Journal of Materials Chemistry C"],["dc.bibliographiccitation.lastpage","3868"],["dc.bibliographiccitation.volume","3"],["dc.contributor.author","Poß, Marieke"],["dc.contributor.author","Napp, Joanna"],["dc.contributor.author","Niehaus, Oliver"],["dc.contributor.author","Pöttgen, Rainer"],["dc.contributor.author","Alves, Frauke"],["dc.contributor.author","Feldmann, Claus"],["dc.date.accessioned","2022-03-01T11:46:08Z"],["dc.date.available","2022-03-01T11:46:08Z"],["dc.date.issued","2015"],["dc.description.abstract","[M 3+ ][AMA] 3− hybrid nanoparticles consist of M 3+ (M = La, Gd) and the fluorescent dye anion [AMA] 3− (AMA: amaranth red) and show multimodal functionality (fluorescence, magnetism) at excellent photostability."],["dc.description.abstract","Lanthanum and gadolinium amaranth-red hybrid nanoparticles consist of an inorganic cation M 3+ (M = La, Gd) and the fluorescent organic dye anion [AMA] 3− (AMA: amaranth red, C 20 H 11 N 2 O 10 S 3 ) that is systematically named (4 E )-3-oxo-4-[(4-sulfonatonaphth-1-yl)hydrazinyliden]naphthalin-2,7-disulfonate (as well named E123, C.I. 16185, Acid Red 27, C-Red 46, Echtrot D, or Food Red 9). M 3+ [AMA] 3− (M = La, Gd) nanoparticles are prepared via aqueous synthesis as highly stable colloidal suspensions with a mean particle diameter of 47 nm. The chemical composition is validated by infrared spectroscopy (FT-IR), energy-dispersive X-ray analysis (EDX), thermogravimetry (TG) and elemental analysis (EA). M 3+ [AMA] 3− (M = La, Gd) shows intense red emission ( λ max = 700 nm) upon excitation at 400–650 nm. Even after 15 hours of UV irradiation (310 nm), the nanoparticles do not show any significant photobleaching. Based on its red fluorescence and its Gd 3+ -based magnetism, especially, Gd 3+ [AMA] 3− nanoparticles can be interesting as a multimodal contrast agent for biomedical applications or as a magneto-optical marker in polymers. This holds even more in view of biocompatibility, high dye load (79 wt%), excellent photostability, and water-based synthesis of the M 3+ [AMA] 3− (M = La, Gd) inorganic–organic hybrid nanoparticles."],["dc.description.abstract","[M 3+ ][AMA] 3− hybrid nanoparticles consist of M 3+ (M = La, Gd) and the fluorescent dye anion [AMA] 3− (AMA: amaranth red) and show multimodal functionality (fluorescence, magnetism) at excellent photostability."],["dc.description.abstract","Lanthanum and gadolinium amaranth-red hybrid nanoparticles consist of an inorganic cation M 3+ (M = La, Gd) and the fluorescent organic dye anion [AMA] 3− (AMA: amaranth red, C 20 H 11 N 2 O 10 S 3 ) that is systematically named (4 E )-3-oxo-4-[(4-sulfonatonaphth-1-yl)hydrazinyliden]naphthalin-2,7-disulfonate (as well named E123, C.I. 16185, Acid Red 27, C-Red 46, Echtrot D, or Food Red 9). M 3+ [AMA] 3− (M = La, Gd) nanoparticles are prepared via aqueous synthesis as highly stable colloidal suspensions with a mean particle diameter of 47 nm. The chemical composition is validated by infrared spectroscopy (FT-IR), energy-dispersive X-ray analysis (EDX), thermogravimetry (TG) and elemental analysis (EA). M 3+ [AMA] 3− (M = La, Gd) shows intense red emission ( λ max = 700 nm) upon excitation at 400–650 nm. Even after 15 hours of UV irradiation (310 nm), the nanoparticles do not show any significant photobleaching. Based on its red fluorescence and its Gd 3+ -based magnetism, especially, Gd 3+ [AMA] 3− nanoparticles can be interesting as a multimodal contrast agent for biomedical applications or as a magneto-optical marker in polymers. This holds even more in view of biocompatibility, high dye load (79 wt%), excellent photostability, and water-based synthesis of the M 3+ [AMA] 3− (M = La, Gd) inorganic–organic hybrid nanoparticles."],["dc.identifier.doi","10.1039/C5TC00413F"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103574"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-531"],["dc.relation.eissn","2050-7534"],["dc.relation.issn","2050-7526"],["dc.title","M 3+ [amaranth red] 3− (M = La, Gd): a novel sulfonate-based inorganic–organic hybrid nanomaterial for multimodal imaging"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","7329"],["dc.bibliographiccitation.issue","23"],["dc.bibliographiccitation.journal","Journal of the American Chemical Society"],["dc.bibliographiccitation.lastpage","7336"],["dc.bibliographiccitation.volume","137"],["dc.contributor.author","Heck, Joachim G."],["dc.contributor.author","Napp, Joanna"],["dc.contributor.author","Simonato, Sara"],["dc.contributor.author","Moellmer, Jens"],["dc.contributor.author","Lange, Marcus"],["dc.contributor.author","Reichardt, Holger Michael"],["dc.contributor.author","Staudt, Reiner"],["dc.contributor.author","Alves, Frauke"],["dc.contributor.author","Feldmann, Claus"],["dc.date.accessioned","2018-11-07T09:55:49Z"],["dc.date.available","2018-11-07T09:55:49Z"],["dc.date.issued","2015"],["dc.description.abstract","Phosphate-based inorganic-organic hybrid nanoparticles (IOH-NPs) with the general composition [M](2+)[R-function(O)PO3](2-) (M = ZrO, Mg2O; R = functional organic group) show multipurpose and multifunctional properties. If [R-function(O))PO3](2-) is a fluorescent dye anion ([RdyeOPO3](2-)), the IOH-NPs show blue, green, red, and near-infrared fluorescence. This is shown for [ZrO](2+)[PUP](2-), [ZrO](2+)[MFP](2-), [ZrO](2+)[RRP](2-), and [ZrO](2+)[DUT](2-) (PUP = phenylumbelliferon phosphate, MFP = methylfluorescein phosphate, RRP = resorufin phosphate, DUT = Dyomics-647 uridine triphosphate). With pharmaceutical agents as functional anions ([RdrugOPO3](2-)), drug transport and release of anti-inflammatory ([ZrO](2+)[BMP](2-)) and antitumor agents ([ZrO](2+)[FdUMP](2-)) with an up to 80% load of active drug is possible (BMP = betamethason phosphate, FdUMP = 5'-fluoro-2'-deoxyuridine 5'-monophosphate). A combination of fluorescent dye and drug anions is possible as well and shown for [ZrO](2+)[BMP](2-)(0.996)[DUT](2-)(0.004). Merging of functional anions, in general, results in [ZrO](2+)([RdrugOPO3](1-x)[RdyeOPO3](x))(2-) nanoparticles and is highly relevant for theranostics. Amine-based functional anions in [MgO](2+)[RaminePO3](2-) IOH-NPs, finally, show CO2 sorption (up to 180 mg g(-1)) and can be used for CO2/N-2 separation (selectivity up to alpha = 23). This includes aminomethyl phosphonate [AMP](2-), 1-aminoethyl phosphonate [1AEP](2-), 2-aminoethyl phosphonate [2AEP](2-), aminopropyl phosphonate [APP](2-), and aminobutyl phosphonate [ABP](2-). All [M](2+)[R-function(O)PO3](2-) IOH-NPs are prepared via noncomplex synthesis in water, which facilitates practical handling and which is optimal for biomedical application. In sum, all IOH-NPs have very similar chemical compositions but can address a variety of different functions, including fluorescence, drug delivery, and CO2 sorption."],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft (DFG)"],["dc.identifier.doi","10.1021/jacs.5b01172"],["dc.identifier.isi","000356753700018"],["dc.identifier.pmid","26018463"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/36833"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Chemical Soc"],["dc.relation.issn","0002-7863"],["dc.title","Multifunctional Phosphate-Based Inorganic-Organic Hybrid Nanoparticles"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3547"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Chemistry of Materials"],["dc.bibliographiccitation.lastpage","3554"],["dc.bibliographiccitation.volume","29"],["dc.contributor.author","Poß, Marieke"],["dc.contributor.author","Tower, Robert J."],["dc.contributor.author","Napp, Joanna"],["dc.contributor.author","Appold, Lia Christina"],["dc.contributor.author","Lammers, Twan"],["dc.contributor.author","Alves, Frauke"],["dc.contributor.author","Glüer, Claus-Christian"],["dc.contributor.author","Boretius, Susann"],["dc.contributor.author","Feldmann, Claus"],["dc.date.accessioned","2018-10-10T11:51:12Z"],["dc.date.available","2018-10-10T11:51:12Z"],["dc.date.issued","2017"],["dc.description.abstract","Multimodal contrast agents with high biocompatibility and biodegradability, as well as low material complexity, are in great demand for clinical diagnostics at different scales of resolution and/or for translating preclinical diagnosis into intraoperative imaging. Multimodality, however, often results in multicomponent and multistructured materials with complexity becoming a severe restriction for synthesis, approval, and use in routine clinical practice. Here, we present sulfonate-based saline [GdO]+[ICG]− (ICG, indocyanine green) inorganic-organic hybrid nanoparticles (IOH-NPs with an inorganic [GdO]+ cation and an organic [ICG]− anion) as a novel, multimodality contrast agent for optical, photoacoustic, and magnetic resonance imaging (OI, PAI, MRI). [GdO]+[ICG]− IOH-NPs have a plain composition based on clinically used constituents and are prepared as an insoluble saline compound in water. The high [ICG]− content (81 wt %) ensures intense near-infrared emission (780–840 nm) and a strong photoacoustic signal. First, in vitro studies demonstrate longer detectability and greater emission intensity for [GdO]+[ICG]− IOH-NP suspensions than for ICG solutions, as well as a reduced toxicity compared to that of Gd-DTPA, a standard MRI contrast agent. Conceptual in vivo studies confirm the utility of the [GdO]+[ICG]− IOH-NPs for optical and magnetic resonance imaging with a T1 relaxivity better than that of Gd-DTPA. Taken together, [GdO]+[ICG]− represents a new compound and nanomaterial that can be highly interesting as a multimodal contrast agent."],["dc.identifier.doi","10.1021/acs.chemmater.6b05406"],["dc.identifier.gro","629524"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/15960"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.title","Multimodal [GdO]+[ICG]− Nanoparticles for Optical, Photoacoustic, and Magnetic Resonance Imaging"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Nanoscale"],["dc.contributor.author","Ritschel, Christian"],["dc.contributor.author","Napp, Joanna"],["dc.contributor.author","Alves, Frauke"],["dc.contributor.author","Feldmann, Claus"],["dc.date.accessioned","2022-11-01T10:16:49Z"],["dc.date.available","2022-11-01T10:16:49Z"],["dc.date.issued","2022"],["dc.description.abstract","Nanoparticle dissolution is monitored\n via\n a fluorescence-colour shift. Intact solid nanoparticles show red emission, whereas green emission indicates nanoparticle dissolution. As a proof-of-concept, this is also shown\n in vitro\n ."],["dc.description.abstract","[La(OH)]\n 2+\n [ICG]\n −\n 2\n and [La(OH)]\n 2+\n 2\n [PTC]\n 4−\n inorganic–organic hybrid nanoparticles (IOH-NPs) with indocyanine green (ICG) and perylene-3,4,9,10-tetracarboxylate (PTC) as fluorescent dye anions are used for emission-based monitoring of the dissolution of nanoparticles. Whereas ICG shows a deep red emission in the solid [La(OH)]\n 2+\n [ICG]\n −\n 2\n IOH-NPs, the emission of PTC in the solid [La(OH)]\n 2+\n 2\n [PTC]\n 4−\n IOH-NPs is completely quenched due to π-stacking. After nanoparticle dissolution, the emission of freely dissolved ICG is weak, whereas freely dissolved PTC shows intense green emission. We report on the synthesis of IOH-NPs and nanoparticle characterization as well as on the fluorescence properties and how to avoid undesirable energy transfer between different fluorescent dyes. The emission shift from red (intact solid nanoparticles) to green (freely dissolved dye anions), indicating nanoparticle dissolution, is shown for aqueous systems and verified\n in vitro\n . Based on this first proof-of-the-concept, the IOH-NP marker system can be interesting to monitor nanoparticle dissolution in cells and tissues of small animals and to evaluate cell processes and/or drug-delivery strategies."],["dc.description.sponsorship"," Deutsche Forschungsgemeinschaft https://doi.org/10.13039/501100001659"],["dc.identifier.doi","10.1039/D2NR03078K"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/116661"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-605"],["dc.relation.eissn","2040-3372"],["dc.relation.issn","2040-3364"],["dc.rights.uri","http://creativecommons.org/licenses/by/3.0/"],["dc.title","Monitoring nanoparticle dissolution\n via\n fluorescence-colour shift"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","9417"],["dc.bibliographiccitation.issue","67"],["dc.bibliographiccitation.journal","Chemical Communications"],["dc.bibliographiccitation.lastpage","9420"],["dc.bibliographiccitation.volume","58"],["dc.contributor.author","Sabljo, Kristina"],["dc.contributor.author","Napp, Joanna"],["dc.contributor.author","Alves, Frauke"],["dc.contributor.author","Feldmann, Claus"],["dc.date.accessioned","2022-09-01T09:50:11Z"],["dc.date.available","2022-09-01T09:50:11Z"],["dc.date.issued","2022"],["dc.description.abstract","[La(OH)\n 2\n ]\n +\n [ARS]\n −\n inorganic–organic hybrid nanoparticles (ARS: alizarin red S) exhibit pH-dependent absorption and pH-dependent emission, allowing to monitor nanoparticle internalization in cells and the intracellular pH."],["dc.description.abstract","Saline inorganic–organic hybrid nanoparticles (IOH-NPs) [La(OH)\n 2\n ]\n +\n [ARS]\n −\n (ARS: alizarin red S) are prepared in water as a new compound (particle size: 47 ± 7 nm, ARS load: 65 wt%). The IOH-NPs not only show a pH-dependent absorption colour but also a pH-dependent fluorescence with green emission at pH 5.0–9.0 and red emission at pH < 4.5. According to first\n in vitro\n studies, the pH-dependend fluorescence can be used to monitor nanoparticle internalization in cells as well as the respective intracellular pH."],["dc.description.sponsorship"," Deutsche Forschungsgemeinschaft https://doi.org/10.13039/501100001659"],["dc.identifier.doi","10.1039/D2CC01507B"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/113641"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-597"],["dc.relation.eissn","1364-548X"],["dc.relation.issn","1359-7345"],["dc.rights.uri","http://creativecommons.org/licenses/by/3.0/"],["dc.title","pH-Dependent fluorescence of [La(OH)\n 2\n ]\n +\n [ARS]\n −\n hybrid nanoparticles for intracellular pH-sensing"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]