Hübscher, Daniela

[["dc.bibliographiccitation.artnumber","13"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Basic Research in Cardiology"],["dc.bibliographiccitation.volume","117"],["dc.contributor.author","Haupt, Luis Peter"],["dc.contributor.author","Rebs, Sabine"],["dc.contributor.author","Maurer, Wiebke"],["dc.contributor.author","Hübscher, Daniela"],["dc.contributor.author","Tiburcy, Malte"],["dc.contributor.author","Pabel, Steffen"],["dc.contributor.author","Maus, Andreas"],["dc.contributor.author","Köhne, Steffen"],["dc.contributor.author","Tappu, Rewati"],["dc.contributor.author","Haas, Jan"],["dc.contributor.author","Streckfuss-Bömeke, Katrin"],["dc.date.accessioned","2022-04-01T10:01:09Z"],["dc.date.available","2022-04-01T10:01:09Z"],["dc.date.issued","2022"],["dc.description.abstract","Abstract Cancer therapies with anthracyclines have been shown to induce cardiovascular complications. The aims of this study were to establish an in vitro induced pluripotent stem cell model (iPSC) of anthracycline-induced cardiotoxicity (ACT) from patients with an aggressive form of B-cell lymphoma and to examine whether doxorubicin (DOX)-treated ACT-iPSC cardiomyocytes (CM) can recapitulate the clinical features exhibited by patients, and thus help uncover a DOX-dependent pathomechanism. ACT-iPSC CM generated from individuals with CD20 + B-cell lymphoma who had received high doses of DOX and suffered cardiac dysfunction were studied and compared to control-iPSC CM from cancer survivors without cardiac symptoms. In cellular studies, ACT-iPSC CM were persistently more susceptible to DOX toxicity including augmented disorganized myofilament structure, changed mitochondrial shape, and increased apoptotic events. Consistently, ACT-iPSC CM and cardiac fibroblasts isolated from fibrotic human ACT myocardium exhibited higher DOX-dependent reactive oxygen species. In functional studies, Ca 2+ transient amplitude of ACT-iPSC CM was reduced compared to control cells, and diastolic sarcoplasmic reticulum Ca 2+ leak was DOX-dependently increased. This could be explained by overactive CaMKIIδ in ACT CM. Together with DOX-dependent augmented proarrhythmic cellular triggers and prolonged action potentials in ACT CM, this suggests a cellular link to arrhythmogenic events and contractile dysfunction especially found in ACT engineered human myocardium. CamKIIδ inhibition prevented proarrhythmic triggers in ACT. In contrast, control CM upregulated SERCA2a expression in a DOX-dependent manner, possibly to avoid heart failure conditions. In conclusion, we developed the first human patient-specific stem cell model of DOX-induced cardiac dysfunction from patients with B-cell lymphoma. Our results suggest that DOX-induced stress resulted in arrhythmogenic events associated with contractile dysfunction and finally in heart failure after persistent stress activation in ACT patients."],["dc.identifier.doi","10.1007/s00395-022-00918-7"],["dc.identifier.pii","918"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/105613"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-530"],["dc.relation.eissn","1435-1803"],["dc.relation.issn","0300-8428"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Doxorubicin induces cardiotoxicity in a pluripotent stem cell model of aggressive B cell lymphoma cancer patients"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Human Gene Therapy"],["dc.bibliographiccitation.volume","20"],["dc.contributor.author","Wolf, F."],["dc.contributor.author","Becker, A."],["dc.contributor.author","Streckfuss-Boemeke, Katrin"],["dc.contributor.author","Huebscher, Daniela"],["dc.contributor.author","Field, Loren J."],["dc.contributor.author","Dressel, Ralf"],["dc.contributor.author","Hasenfuß, Gerd"],["dc.contributor.author","Guan, Kaomei"],["dc.date.accessioned","2018-11-07T11:22:43Z"],["dc.date.available","2018-11-07T11:22:43Z"],["dc.date.issued","2009"],["dc.format.extent","1406"],["dc.identifier.isi","000271441000171"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/56035"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Mary Ann Liebert Inc"],["dc.publisher.place","New rochelle"],["dc.relation.conference","Combined Meeting of the 17th European-Society-of-Gene-and-Cell-Therapy/16th German-Society-for-Gene-Therapy/4th German-Society-for-Stem-Cell-Research"],["dc.relation.eventlocation","Hannover, GERMANY"],["dc.relation.issn","1043-0342"],["dc.title","Characterization and maintenance of adult spermatogonial stem cell culture"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Human Gene Therapy"],["dc.bibliographiccitation.volume","20"],["dc.contributor.author","Streckfuss-Boemeke, Katrin"],["dc.contributor.author","Jende, Joerg"],["dc.contributor.author","Huebscher, Daniela"],["dc.contributor.author","Hasenfuß, Gerd"],["dc.contributor.author","Guan, Kaomei"],["dc.date.accessioned","2018-11-07T11:22:44Z"],["dc.date.available","2018-11-07T11:22:44Z"],["dc.date.issued","2009"],["dc.format.extent","1484"],["dc.identifier.isi","000271441000385"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/56038"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Mary Ann Liebert Inc"],["dc.publisher.place","New rochelle"],["dc.relation.conference","Combined Meeting of the 17th European-Society-of-Gene-and-Cell-Therapy/16th German-Society-for-Gene-Therapy/4th German-Society-for-Stem-Cell-Research"],["dc.relation.eventlocation","Hannover, GERMANY"],["dc.relation.issn","1043-0342"],["dc.title","Generation of hepatic-like cells from multipotent adult mouse germline stem cells"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1304"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Circulation Research"],["dc.bibliographiccitation.lastpage","1311"],["dc.bibliographiccitation.volume","116"],["dc.contributor.author","Perera, Ruwan K."],["dc.contributor.author","Sprenger, Julia U."],["dc.contributor.author","Steinbrecher, Julia H."],["dc.contributor.author","Hübscher, Daniela"],["dc.contributor.author","Lehnart, Stephan Elmar"],["dc.contributor.author","Abesser, Marco"],["dc.contributor.author","Schuh, Kai"],["dc.contributor.author","El-Armouche, Ali"],["dc.contributor.author","Nikolaev, Viacheslav O."],["dc.date.accessioned","2018-05-07T11:53:29Z"],["dc.date.available","2018-05-07T11:53:29Z"],["dc.date.issued","2015"],["dc.description.abstract","Cyclic nucleotides are second messengers that regulate cardiomyocyte function through compartmentalized signaling in discrete subcellular microdomains. However, the role of different microdomains and their changes in cardiac disease are not well understood."],["dc.identifier.doi","10.1161/CIRCRESAHA.116.306082"],["dc.identifier.pmid","25688144"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/14623"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/82"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation","SFB 1002 | A01: cAMP- und cGMP- Mikrodomänen bei Herzhypertrophie und Insuffizienz"],["dc.relation","SFB 1002 | A09: Lokale molekulare Nanodomänen-Regulation der kardialen Ryanodin-Rezeptor-Funktion"],["dc.relation.doi","10.1161/CIRCRESAHA.116.306082"],["dc.relation.eissn","1524-4571"],["dc.relation.issn","1524-4571"],["dc.relation.workinggroup","RG El-Armouche"],["dc.relation.workinggroup","RG Lehnart (Cellular Biophysics and Translational Cardiology Section)"],["dc.relation.workinggroup","RG Nikolaev (Cardiovascular Research Center)"],["dc.title","Microdomain switch of cGMP-regulated phosphodiesterases leads to ANP-induced augmentation of β-adrenoceptor-stimulated contractility in early cardiac hypertrophy"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","101746"],["dc.bibliographiccitation.journal","Stem Cell Research"],["dc.bibliographiccitation.volume","44"],["dc.contributor.author","Hübscher, Daniela"],["dc.contributor.author","Rebs, Sabine"],["dc.contributor.author","Maurer, Wiebke"],["dc.contributor.author","Ghadri, Jelena R."],["dc.contributor.author","Dressel, Ralf"],["dc.contributor.author","Templin, Christian"],["dc.contributor.author","Streckfuss-Bömeke, Katrin"],["dc.date.accessioned","2020-12-10T15:21:15Z"],["dc.date.available","2020-12-10T15:21:15Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1016/j.scr.2020.101746"],["dc.identifier.issn","1873-5061"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/72964"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Generation of iPSC-lines from two independent Takotsubo syndrome patients with recurrent Takotsubo events"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","343"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","HLA"],["dc.bibliographiccitation.lastpage","344"],["dc.bibliographiccitation.volume","89"],["dc.contributor.author","Dressel, Ralf"],["dc.contributor.author","Huebscher, Daniela"],["dc.contributor.author","Monecke, Sebastian"],["dc.contributor.author","Elsner, Leslie"],["dc.contributor.author","Borchert, Thomas"],["dc.contributor.author","Cyganek, Lukas"],["dc.contributor.author","Stauske, Michael"],["dc.contributor.author","Hejazi, Maryam"],["dc.contributor.author","Uhrberg, Markus"],["dc.contributor.author","Guan, Kaomei"],["dc.contributor.author","Streckfuss-Boemeke, Katrin"],["dc.date.accessioned","2018-11-07T10:23:29Z"],["dc.date.available","2018-11-07T10:23:29Z"],["dc.date.issued","2017"],["dc.identifier.isi","000400973300013"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/42464"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Wiley"],["dc.publisher.place","Hoboken"],["dc.relation.issn","2059-2310"],["dc.relation.issn","2059-2302"],["dc.title","HUMAN CARDIOMYOCYTES DERIVED FROM INDUCED PLURIPOTENT STEM CELLS ARE TARGETS FOR ACTIVATED AUTOLOGOUS AND ALLOGENEIC NATURAL KILLER CELLS"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2618"],["dc.bibliographiccitation.issue","33"],["dc.bibliographiccitation.journal","European heart journal"],["dc.bibliographiccitation.lastpage","2629"],["dc.bibliographiccitation.volume","34"],["dc.contributor.author","Streckfuss-Boemeke, Katrin"],["dc.contributor.author","Wolf, Frieder"],["dc.contributor.author","Azizian, Azadeh"],["dc.contributor.author","Stauske, Michael"],["dc.contributor.author","Tiburcy, Malte"],["dc.contributor.author","Wagner, Stefan"],["dc.contributor.author","Huebscher, Daniela"],["dc.contributor.author","Dressel, Ralf"],["dc.contributor.author","Chen, Simin"],["dc.contributor.author","Jende, Jörg"],["dc.contributor.author","Wulf, Gerald"],["dc.contributor.author","Lorenz, Verena"],["dc.contributor.author","Schoen, Michael P."],["dc.contributor.author","Maier, Lars S."],["dc.contributor.author","Zimmermann, Wolfram-Hubertus"],["dc.contributor.author","Hasenfuß, Gerd"],["dc.contributor.author","Guan, Kaomei"],["dc.date.accessioned","2017-09-07T11:47:10Z"],["dc.date.available","2017-09-07T11:47:10Z"],["dc.date.issued","2013"],["dc.description.abstract","Induced pluripotent stem cells (iPSCs) provide a unique opportunity for the generation of patient-specific cells for use in disease modelling, drug screening, and regenerative medicine. The aim of this study was to compare human-induced pluripotent stem cells (hiPSCs) derived from different somatic cell sources regarding their generation efficiency and cardiac differentiation potential, and functionalities of cardiomyocytes. We generated hiPSCs from hair keratinocytes, bone marrow mesenchymal stem cells (MSCs), and skin fibroblasts by using two different virus systems. We show that MSCs and fibroblasts are more easily reprogrammed than keratinocytes. This corresponds to higher methylation levels of minimal promoter regions of the OCT4 and NANOG genes in keratinocytes than in MSCs and fibroblasts. The success rate and reprogramming efficiency was significantly higher by using the STEMCCA system than the OSNL system. All analysed hiPSCs are pluripotent and show phenotypical characteristics similar to human embryonic stem cells. We studied the cardiac differentiation efficiency of generated hiPSC lines (n 24) and found that MSC-derived hiPSCs exhibited a significantly higher efficiency to spontaneously differentiate into beating cardiomyocytes when compared with keratinocyte-, and fibroblast-derived hiPSCs. There was no significant difference in the functionalities of the cardiomyocytes derived from hiPSCs with different origins, showing the presence of pacemaker-, atrial-, ventricular- and Purkinje-like cardiomyocytes, and exhibiting rhythmic Ca-2 transients and Ca-2 sparks in hiPSC-derived cardiomyocytes. Furthermore, spontaneously and synchronously beating and force-developing engineered heart tissues were generated. Human-induced pluripotent stem cells can be reprogrammed from all three somatic cell types, but with different efficiency. All analysed iPSCs can differentiate into cardiomyocytes, and the functionalities of cardiomyocytes derived from different cell origins are similar. However, MSC-derived hiPSCs revealed a higher cardiac differentiation efficiency than keratinocyte- and fibroblast-derived hiPSCs."],["dc.identifier.doi","10.1093/eurheartj/ehs203"],["dc.identifier.gro","3142288"],["dc.identifier.isi","000324367700013"],["dc.identifier.pmid","22798560"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/6620"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/61"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation","SFB 1002 | A04: Patienten-spezifische induzierte pluripotente Stammzellen zur funktionellen Untersuchung von Ryanodinrezeptor-Mutationen"],["dc.relation.issn","0195-668X"],["dc.relation.workinggroup","RG Dressel"],["dc.relation.workinggroup","RG Guan (Application of patient-specific induced pluripotent stem cells in disease modelling)"],["dc.relation.workinggroup","RG Hasenfuß (Transition zur Herzinsuffizienz)"],["dc.relation.workinggroup","RG L. Maier (Experimentelle Kardiologie)"],["dc.relation.workinggroup","RG Tiburcy (Stem Cell Disease Modeling)"],["dc.relation.workinggroup","RG Zimmermann (Engineered Human Myocardium)"],["dc.title","Comparative study of human-induced pluripotent stem cells derived from bone marrow cells, hair keratinocytes, and skin fibroblasts"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]