Nikolaev, Viacheslav O.

[["dc.bibliographiccitation.firstpage","121"],["dc.bibliographiccitation.lastpage","129"],["dc.bibliographiccitation.seriesnr","1020"],["dc.contributor.author","Götz, Konrad R."],["dc.contributor.author","Nikolaev, Viacheslav O."],["dc.contributor.editor","Krieg, Thomas"],["dc.contributor.editor","Lukowski, Robert"],["dc.date.accessioned","2019-02-18T14:06:51Z"],["dc.date.available","2019-02-18T14:06:51Z"],["dc.date.issued","2013"],["dc.description.abstract","Förster resonance energy transfer (FRET)-based biosensors are powerful tools for real-time monitoring of signaling events in intact cells using fluorescence microscopy. Here, we describe a highly sensitive method which allows FRET-based measurements of the second messenger cGMP in adult mouse ventricular myocytes. Such measurements have been challenging before, primarily due to relatively low cGMP concentrations in cardiomyocytes and limited sensitivity of the available biosensors. With our new technique, one can reliably measure dynamic changes in cGMP upon stimulation of myocytes with natriuretic peptides and other physiological and pharmacological ligands."],["dc.identifier.doi","10.1007/978-1-62703-459-3_7"],["dc.identifier.pmid","23709029"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/57580"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/19"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.publisher","Humana Press"],["dc.publisher.place","Totowa, NJ"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation","SFB 1002 | A01: cAMP- und cGMP- Mikrodomänen bei Herzhypertrophie und Insuffizienz"],["dc.relation.crisseries","Methods in Molecular Biology"],["dc.relation.doi","10.1007/978-1-62703-459-3"],["dc.relation.isbn","978-1-62703-458-6"],["dc.relation.isbn","978-1-62703-459-3"],["dc.relation.ispartof","Guanylate Cyclase and Cyclic GMP. Methods and Protocols"],["dc.relation.ispartofseries","Methods in Molecular Biology; 1020"],["dc.relation.issn","1064-3745"],["dc.relation.issn","1940-6029"],["dc.relation.workinggroup","RG Nikolaev (Cardiovascular Research Center)"],["dc.title","Advances and Techniques to Measure cGMP in Intact Cardiomyocytes"],["dc.type","book_chapter"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1235"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Circulation Research"],["dc.bibliographiccitation.lastpage","1245"],["dc.bibliographiccitation.volume","114"],["dc.contributor.author","Götz, Konrad R."],["dc.contributor.author","Sprenger, Julia U."],["dc.contributor.author","Perera, Ruwan K."],["dc.contributor.author","Steinbrecher, Julia H."],["dc.contributor.author","Lehnart, Stephan Elmar"],["dc.contributor.author","Kuhn, Michaela"],["dc.contributor.author","Gorelik, Julia"],["dc.contributor.author","Balligand, Jean-Luc"],["dc.contributor.author","Nikolaev, Viacheslav O."],["dc.date.accessioned","2018-05-07T12:50:07Z"],["dc.date.available","2018-05-07T12:50:07Z"],["dc.date.issued","2014"],["dc.description.abstract","3',5'-Cyclic guanosine monophosphate (cGMP) is an important second messenger that regulates cardiac contractility and protects the heart from hypertrophy. However, because of the lack of real-time imaging techniques, specific subcellular mechanisms and spatiotemporal dynamics of cGMP in adult cardiomyocytes are not well understood."],["dc.identifier.doi","10.1161/CIRCRESAHA.114.302437"],["dc.identifier.pmid","24599804"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/14629"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/20"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation","SFB 1002 | A01: cAMP- und cGMP- Mikrodomänen bei Herzhypertrophie und Insuffizienz"],["dc.relation","SFB 1002 | A09: Lokale molekulare Nanodomänen-Regulation der kardialen Ryanodin-Rezeptor-Funktion"],["dc.relation.doi","10.1161/CIRCRESAHA.114.302437"],["dc.relation.eissn","1524-4571"],["dc.relation.issn","1524-4571"],["dc.relation.workinggroup","RG Lehnart (Cellular Biophysics and Translational Cardiology Section)"],["dc.relation.workinggroup","RG Nikolaev (Cardiovascular Research Center)"],["dc.title","Transgenic mice for real-time visualization of cGMP in intact adult cardiomyocytes"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]