Nikolaev, Viacheslav O.

[["dc.bibliographiccitation.issue","21"],["dc.bibliographiccitation.journal","Circulation"],["dc.bibliographiccitation.volume","126"],["dc.contributor.author","Wright, Peter T."],["dc.contributor.author","Diakonov, Ivan"],["dc.contributor.author","Nikolaev, Viacheslav O."],["dc.contributor.author","Lyon, Alexander R."],["dc.contributor.author","Harding, Sian E."],["dc.contributor.author","Gorelik, Julia"],["dc.date.accessioned","2018-11-07T09:03:20Z"],["dc.date.available","2018-11-07T09:03:20Z"],["dc.date.issued","2012"],["dc.identifier.isi","000208885005271"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/24886"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Lippincott Williams & Wilkins"],["dc.publisher.place","Philadelphia"],["dc.relation.issn","1524-4539"],["dc.relation.issn","0009-7322"],["dc.title","Myocardial Gradients in Caveolar Number Modulate Cardiomyocyte Contractile Response to Specific beta 2AR Stimulation"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","22"],["dc.bibliographiccitation.journal","Circulation"],["dc.bibliographiccitation.volume","128"],["dc.contributor.author","O'Hara, Thomas"],["dc.contributor.author","Wright, Peter T."],["dc.contributor.author","Nikolaev, Viacheslav O."],["dc.contributor.author","Gorelik, Julia"],["dc.contributor.author","Trayanova, Natalia A."],["dc.date.accessioned","2018-11-07T09:17:26Z"],["dc.date.available","2018-11-07T09:17:26Z"],["dc.date.issued","2013"],["dc.identifier.isi","000332162901136"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/28172"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Lippincott Williams & Wilkins"],["dc.publisher.place","Philadelphia"],["dc.relation.conference","Scientific Sessions and Resuscitation Science Symposium of the American-Heart-Association"],["dc.relation.eventlocation","Dallas, TX"],["dc.relation.issn","1524-4539"],["dc.relation.issn","0009-7322"],["dc.title","Caveolin-3 Restores Local cAMP Signaling Without Restoring T-Tubules in Response to ss 2 Adrenergic Receptor Stimulation in Heart Failure"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","357"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Circulation Heart Failure"],["dc.bibliographiccitation.lastpage","365"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Lyon, Alexander R."],["dc.contributor.author","Nikolaev, Viacheslav O."],["dc.contributor.author","Miragoli, Michele"],["dc.contributor.author","Sikkel, Markus B."],["dc.contributor.author","Paur, Helen"],["dc.contributor.author","Benard, Ludovic"],["dc.contributor.author","Hulot, Jean-Sebastien"],["dc.contributor.author","Kohlbrenner, Erik"],["dc.contributor.author","Hajjar, Roger J."],["dc.contributor.author","Peters, Nicholas S."],["dc.contributor.author","Korchev, Yuri E."],["dc.contributor.author","Macleod, Ken T."],["dc.contributor.author","Harding, Sian E."],["dc.contributor.author","Gorelik, Julia"],["dc.date.accessioned","2018-11-07T09:10:31Z"],["dc.date.available","2018-11-07T09:10:31Z"],["dc.date.issued","2012"],["dc.description.abstract","Background-Cardiomyocyte surface morphology and T-tubular structure are significantly disrupted in chronic heart failure, with important functional sequelae, including redistribution of sarcolemmal beta(2)-adrenergic receptors (beta(2)AR) and localized secondary messenger signaling. Plasticity of these changes in the reverse remodeled failing ventricle is unknown. We used AAV9.SERCA2a gene therapy to rescue failing rat hearts and measured z-groove index, T-tubule density, and compartmentalized beta(2)AR-mediated cAMP signals, using a combined nanoscale scanning ion conductance microscopy-Forster resonance energy transfer technique. Methods and Results-Cardiomyocyte surface morphology, quantified by z-groove index and T-tubule density, was normalized in reverse-remodeled hearts after SERCA2a gene therapy. Recovery of sarcolemmal microstructure correlated with functional beta(2)AR redistribution back into the z-groove and T-tubular network, whereas minimal cAMP responses were initiated after local beta(2)AR stimulation of crest membrane, as observed in failing cardiomyocytes. Improvement of beta(2)AR localization was associated with recovery of beta AR-stimulated contractile responses in rescued cardiomyocytes. Retubulation was associated with reduced spatial heterogeneity of electrically stimulated calcium transients and recovery of myocardial BIN-1 and TCAP protein expression but not junctophilin-2. Conclusions-In summary, abnormalities of sarcolemmal structure in heart failure show plasticity with reappearance of z-grooves and T-tubules in reverse-remodeled hearts. Recovery of surface topology is necessary for normalization of beta(2)AR location and signaling responses. (Circ Heart Fail. 2012;5:357-365.)"],["dc.identifier.doi","10.1161/CIRCHEARTFAILURE.111.964692"],["dc.identifier.isi","000313577100011"],["dc.identifier.pmid","22456061"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/26510"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Lippincott Williams & Wilkins"],["dc.relation.issn","1941-3289"],["dc.title","Plasticity of Surface Structures and beta(2)-Adrenergic Receptor Localization in Failing Ventricular Cardiomyocytes During Recovery From Heart Failure"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","38"],["dc.bibliographiccitation.journal","Journal of Molecular and Cellular Cardiology"],["dc.bibliographiccitation.lastpage","48"],["dc.bibliographiccitation.volume","67"],["dc.contributor.author","Wright, Peter T."],["dc.contributor.author","Nikolaev, Viacheslav O."],["dc.contributor.author","O'Hara, Thomas"],["dc.contributor.author","Diakonov, Ivan"],["dc.contributor.author","Bhargava, Anamika"],["dc.contributor.author","Tokar, Sergiy"],["dc.contributor.author","Schobesberger, Sophie"],["dc.contributor.author","Shevchuk, Andrew I."],["dc.contributor.author","Sikkel, Markus B."],["dc.contributor.author","Wilkinson, Ross"],["dc.contributor.author","Trayanova, Natalia A."],["dc.contributor.author","Lyon, Alexander R."],["dc.contributor.author","Harding, Sian E."],["dc.contributor.author","Gorelik, Julia"],["dc.date.accessioned","2018-11-07T09:44:34Z"],["dc.date.available","2018-11-07T09:44:34Z"],["dc.date.issued","2014"],["dc.description.abstract","The purpose of this study was to investigate whether caveolin-3 (Cav3) regulates localization of beta(2)-adrenergic receptor (beta(2)AR) and its cAMP signaling in healthy or failing cardiomyocytes. We co-expressed wildtype Cav3 or its dominant-negative mutant (Cav3DN) together with the Forster resonance energy transfer (FRET)-based cAMP sensor Epac2-camps in adult rat ventricular myocytes (ARVMs). FRET and scanning ion conductance microscopy were used to locally stimulate beta(2)-AR and to measure cytosolic cAMP. Cav3 overexpression increased the number of caveolae and decreased the magnitude of beta(2)-AR-cAMP signal. Conversely, Cav3DN expression resulted in an increased beta(2)-AR-cAMP response without altering the whole-cell L-type calcium current. Following local stimulation of Cav3DN-expressing ARVMs, beta(2)-AR response could only be generated in T-tubules. However, the normally compartmentalized beta(2)-AR-cAMP signal became diffuse, similar to the situation observed in heart failure. Finally, overexpression of Cav3 in failing myocytes led to partial beta(2)-AR redistribution back into the T-tubules. In conclusion, Cav3 plays a crucial role for the localization of beta(2)-AR and compartmentation of beta(2)-AR-cAMP signaling to the T-tubules of healthy ARVMs, and overexpression of Cav3 in failing myocytes can partially restore the disrupted localization of these receptors. (C) 2013 The Authors. Published by Elsevier Ltd. All rights reserved."],["dc.identifier.doi","10.1016/j.yjmcc.2013.12.003"],["dc.identifier.isi","000330747600004"],["dc.identifier.pmid","24345421"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/34425"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/75"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation","SFB 1002 | A01: cAMP- und cGMP- Mikrodomänen bei Herzhypertrophie und Insuffizienz"],["dc.relation.issn","1095-8584"],["dc.relation.issn","0022-2828"],["dc.relation.workinggroup","RG Nikolaev (Cardiovascular Research Center)"],["dc.title","Caveolin-3 regulates compartmentation of cardiomyocyte beta2-adrenergic receptor-mediated cAMP signaling"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","546"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Cardiovascular Research"],["dc.bibliographiccitation.lastpage","555"],["dc.bibliographiccitation.volume","115"],["dc.contributor.author","Bastug-Özel, Zeynep"],["dc.contributor.author","Wright, Peter T"],["dc.contributor.author","Kraft, Axel E"],["dc.contributor.author","Pavlovic, Davor"],["dc.contributor.author","Howie, Jacqueline"],["dc.contributor.author","Froese, Alexander"],["dc.contributor.author","Fuller, William"],["dc.contributor.author","Gorelik, Julia"],["dc.contributor.author","Shattock, Michael J"],["dc.contributor.author","Nikolaev, Viacheslav O"],["dc.date.accessioned","2020-12-10T18:18:50Z"],["dc.date.available","2020-12-10T18:18:50Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1093/cvr/cvy221"],["dc.identifier.eissn","1755-3245"],["dc.identifier.issn","0008-6363"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/75100"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Heart failure leads to altered β2-adrenoceptor/cyclic adenosine monophosphate dynamics in the sarcolemmal phospholemman/Na,K ATPase microdomain"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Heart"],["dc.bibliographiccitation.volume","98"],["dc.contributor.author","Wright, Peter T."],["dc.contributor.author","Pannell, L. M. K."],["dc.contributor.author","Nikolaev, V. O."],["dc.contributor.author","Lyon, Alexander R."],["dc.contributor.author","Harding, Sian E."],["dc.contributor.author","Gorelik, Julia"],["dc.date.accessioned","2018-11-07T09:03:39Z"],["dc.date.available","2018-11-07T09:03:39Z"],["dc.date.issued","2012"],["dc.identifier.doi","10.1136/heartjnl-2012-302951.016"],["dc.identifier.isi","000320708800017"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/24942"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Bmj Publishing Group"],["dc.publisher.place","London"],["dc.relation.conference","Joint Spring Meeting of the British-Society-for-Cardiovascular-Research (BSCR) and British-Atherosclerosis-Society (BAS)"],["dc.relation.eventlocation","Manchester, ENGLAND"],["dc.title","IMAGING REGIONAL DIFFERENCES IN BETA-2 ADRENERGIC RECEPTOR FUNCTION AT THE CELLULAR LEVEL"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","21"],["dc.bibliographiccitation.journal","Circulation"],["dc.bibliographiccitation.volume","124"],["dc.contributor.author","Lyon, Alexander R."],["dc.contributor.author","Nikolaev, Viacheslav O."],["dc.contributor.author","Miragoli, Michele"],["dc.contributor.author","Paur, Helen"],["dc.contributor.author","Bernard, Ludovic"],["dc.contributor.author","Hulot, Jean-Sebastien"],["dc.contributor.author","Kohlbrenner, Erik"],["dc.contributor.author","Hajjar, Roger J."],["dc.contributor.author","Peters, Nicholas S."],["dc.contributor.author","Korchev, Yuri E."],["dc.contributor.author","Harding, Sian E."],["dc.contributor.author","Gorelik, Julia"],["dc.date.accessioned","2018-11-07T08:49:43Z"],["dc.date.available","2018-11-07T08:49:43Z"],["dc.date.issued","2011"],["dc.identifier.isi","000299738702370"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/21530"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Lippincott Williams & Wilkins"],["dc.publisher.place","Philadelphia"],["dc.relation.issn","0009-7322"],["dc.title","Plasticity of Surface Structures and Beta 2-adrenergic Receptor Localization in Failing Ventricular Cardiomyocytes During Recovery From Heart Failure"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1235"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Circulation Research"],["dc.bibliographiccitation.lastpage","1245"],["dc.bibliographiccitation.volume","114"],["dc.contributor.author","Götz, Konrad R."],["dc.contributor.author","Sprenger, Julia U."],["dc.contributor.author","Perera, Ruwan K."],["dc.contributor.author","Steinbrecher, Julia H."],["dc.contributor.author","Lehnart, Stephan Elmar"],["dc.contributor.author","Kuhn, Michaela"],["dc.contributor.author","Gorelik, Julia"],["dc.contributor.author","Balligand, Jean-Luc"],["dc.contributor.author","Nikolaev, Viacheslav O."],["dc.date.accessioned","2018-05-07T12:50:07Z"],["dc.date.available","2018-05-07T12:50:07Z"],["dc.date.issued","2014"],["dc.description.abstract","3',5'-Cyclic guanosine monophosphate (cGMP) is an important second messenger that regulates cardiac contractility and protects the heart from hypertrophy. However, because of the lack of real-time imaging techniques, specific subcellular mechanisms and spatiotemporal dynamics of cGMP in adult cardiomyocytes are not well understood."],["dc.identifier.doi","10.1161/CIRCRESAHA.114.302437"],["dc.identifier.pmid","24599804"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/14629"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/20"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation","SFB 1002 | A01: cAMP- und cGMP- Mikrodomänen bei Herzhypertrophie und Insuffizienz"],["dc.relation","SFB 1002 | A09: Lokale molekulare Nanodomänen-Regulation der kardialen Ryanodin-Rezeptor-Funktion"],["dc.relation.doi","10.1161/CIRCRESAHA.114.302437"],["dc.relation.eissn","1524-4571"],["dc.relation.issn","1524-4571"],["dc.relation.workinggroup","RG Lehnart (Cellular Biophysics and Translational Cardiology Section)"],["dc.relation.workinggroup","RG Nikolaev (Cardiovascular Research Center)"],["dc.title","Transgenic mice for real-time visualization of cGMP in intact adult cardiomyocytes"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","21"],["dc.bibliographiccitation.journal","Circulation"],["dc.bibliographiccitation.volume","126"],["dc.contributor.author","Wright, Peter T."],["dc.contributor.author","Nikolaev, Viacheslav O."],["dc.contributor.author","Tokar, Sergiy"],["dc.contributor.author","Bhargava, Anamika"],["dc.contributor.author","Diakonov, Ivan"],["dc.contributor.author","Sikkel, Markus B."],["dc.contributor.author","Lyon, Alexander R."],["dc.contributor.author","Harding, Sian E."],["dc.contributor.author","Gorelik, Julia"],["dc.date.accessioned","2018-11-07T09:03:20Z"],["dc.date.available","2018-11-07T09:03:20Z"],["dc.date.issued","2012"],["dc.identifier.isi","000208885005277"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/24885"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Lippincott Williams & Wilkins"],["dc.publisher.place","Philadelphia"],["dc.relation.issn","1524-4539"],["dc.relation.issn","0009-7322"],["dc.title","Caveolin3 Depletion Modifies beta 2AR-cAMP Responses at Both Cellular and Sub-Cellular Levels in Cardiomyocytes"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","697"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Circulation"],["dc.bibliographiccitation.lastpage","+"],["dc.bibliographiccitation.volume","126"],["dc.contributor.author","Paur, Helen"],["dc.contributor.author","Wright, Peter T."],["dc.contributor.author","Sikkel, Markus B."],["dc.contributor.author","Tranter, Matthew H."],["dc.contributor.author","Mansfield, Catherine"],["dc.contributor.author","O'Gara, Peter"],["dc.contributor.author","Stuckey, Daniel J."],["dc.contributor.author","Nikolaev, Viacheslav O."],["dc.contributor.author","Diakonov, Ivan"],["dc.contributor.author","Pannell, Laura"],["dc.contributor.author","Gong, Haibin"],["dc.contributor.author","Sun, Hong"],["dc.contributor.author","Peters, Nicholas S."],["dc.contributor.author","Petrou, Mario"],["dc.contributor.author","Zheng, Zhaolun"],["dc.contributor.author","Gorelik, Julia"],["dc.contributor.author","Lyon, Alexander R."],["dc.contributor.author","Harding, Sian L."],["dc.date.accessioned","2018-11-07T09:07:17Z"],["dc.date.available","2018-11-07T09:07:17Z"],["dc.date.issued","2012"],["dc.description.abstract","Background-Takotsubo cardiomyopathy is an acute heart failure syndrome characterized by myocardial hypocontractility from the mid left ventricle to the apex. It is precipitated by extreme stress and can be triggered by intravenous catecholamine administration, particularly epinephrine. Despite its grave presentation, Takotsubo cardiomyophathy is rapidly reversible, with generally good prognosis. We hypothesized that this represents switching of epinephrine signaling through the pleiotropic beta(2)-adrenergic receptor (beta(2)AR) from canonical stimulatory G-protein-activated cardiostimulant to inhibitory G-protein-activated cardiodepressant pathways. Methods and Results-We describe an in vivo rat model in which a high intravenous epinephrine, but not norepinephrine, bolus produces the characteristic reversible apical depression of myocardial contraction coupled with basal hypercontractility. The effect is prevented via G(i) inactivation by pertussis toxin pretreatment. beta(2)AR number and functional responses were greater in isolated apical cardiomyocytes than in basal cardiomyocytes, which confirmed the higher apical sensitivity and response to circulating epinephrine. In vivo studies demonstrated high-dose epinephrine can induce direct cardiomyocytes cardiodepression and cardioprotection in a beta 2AR-Gi-dependent manner. Preventing epinephereine-G(i) effects increased mortality in the Takotsubo model, whereas beta-blockers that activate beta(2)AR-G(i) exacerbated the epinephrine-dependent negative inotropic effects without further deaths. In contrast, levosimendan rescued the acure cardiac dysfunction without increased mortality. Conclusions-We suggest that biased agonism of epinephrine for beta(2)-AR-G(s) at low concentrations and for G(i) at high concentrations underpins the acute apical cardiopression observed in Takotsubo cardiomyopathy, with an apical-basal gradient in beta(2)ARs explaining the differential regional responses. We suggest this epinephrine-specific beta(2)AR-G(i) signaling may have evolved as a cardioprotective stategy to limit catecholamine-induced myocardial toxicity during acute stress (Circulation. 2012; 126:697-706.)"],["dc.identifier.doi","10.1161/CIRCULATIONAHA.112.111591"],["dc.identifier.isi","000307472600016"],["dc.identifier.pmid","22732314"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/25758"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Lippincott Williams & Wilkins"],["dc.relation.issn","1524-4539"],["dc.relation.issn","0009-7322"],["dc.title","High Levels of Circulating Epinehrine Trigger Apical Cardiodepression in a beta(2)-Adrenergic Receptor/G(i)-Dependent Manner A New Model of Takotsubo Cardiomyopathy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]