Bohne, Wolfgang

[["dc.bibliographiccitation.firstpage","517"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Antimicrobial Agents and Chemotherapy"],["dc.bibliographiccitation.lastpage","521"],["dc.bibliographiccitation.volume","54"],["dc.contributor.author","Bajohr, Lara Liv"],["dc.contributor.author","Ma, Ling"],["dc.contributor.author","Platte, Christian"],["dc.contributor.author","Liesenfeld, Oliver"],["dc.contributor.author","Tietze, Lutz Friedjan"],["dc.contributor.author","Gross, Uwe"],["dc.contributor.author","Bohne, Wolfgang"],["dc.date.accessioned","2018-11-07T08:47:00Z"],["dc.date.available","2018-11-07T08:47:00Z"],["dc.date.issued","2010"],["dc.description.abstract","1-Hydroxy-2-dodecyl-4(1H) quinolone (HDQ) was recently identified as a Toxoplasma gondii inhibitor. We describe here two novel 1-hydroxyquinolones, which displayed 50% inhibitory concentrations 10- and 5-fold lower than that of HDQ. In a mouse model of acute toxoplasmosis, these two compounds and HDQ reduced the percentages of infected peritoneal cells and decreased the parasite loads in lungs and livers. Compound B showed a tendency toward lowering parasite loads in brains in a mouse model of toxoplasmic encephalitis."],["dc.identifier.doi","10.1128/AAC.01001-09"],["dc.identifier.isi","000272931200069"],["dc.identifier.pmid","19884369"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/20837"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Soc Microbiology"],["dc.relation.issn","0066-4804"],["dc.title","In Vitro and In Vivo Activities of 1-Hydroxy-2-Alkyl-4(1H)Quinolone Derivatives against Toxoplasma gondii"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Tropical Medicine & International Health"],["dc.bibliographiccitation.volume","20"],["dc.contributor.author","Deutschmann, S."],["dc.contributor.author","Bohne, Wolfgang"],["dc.contributor.author","Mujuni, F."],["dc.contributor.author","Kalluvya, S."],["dc.contributor.author","Mshana, Stephen E."],["dc.contributor.author","Gross, U."],["dc.contributor.author","Mueller, A."],["dc.date.accessioned","2018-11-07T09:52:27Z"],["dc.date.available","2018-11-07T09:52:27Z"],["dc.date.issued","2015"],["dc.format.extent","287"],["dc.identifier.isi","000360758801298"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/36128"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.publisher.place","Hoboken"],["dc.relation.issn","1365-3156"],["dc.relation.issn","1360-2276"],["dc.title","Low prevalence of Neisseria gonorrhoeae infections and no evidence of resistance against third generation cephalosporins in a cohort of HIV positive patients from a tertiary hospital in Tanzania"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","International Journal of Medical Microbiology"],["dc.bibliographiccitation.volume","293"],["dc.contributor.author","Meyer, J."],["dc.contributor.author","Riebe, R."],["dc.contributor.author","Conraths, Franz J."],["dc.contributor.author","Bohne, Wolfgang"],["dc.contributor.author","Rohn, K."],["dc.contributor.author","Peters, M."],["dc.contributor.author","Schares, Gereon"],["dc.date.accessioned","2018-11-07T10:50:40Z"],["dc.date.available","2018-11-07T10:50:40Z"],["dc.date.issued","2004"],["dc.format.extent","110"],["dc.identifier.isi","000222589900176"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/48708"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Urban & Fischer Verlag"],["dc.publisher.place","Jena"],["dc.relation.conference","21st Congress of the German-Society-of-Parasitology"],["dc.relation.eventlocation","Univ Wurzburg, Wurzburg, GERMANY"],["dc.relation.issn","1438-4221"],["dc.title","Comparative examination of the expression of tissue cyst markers in Hammondia sp isolated from dogs and foxes"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","229"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","International Journal for Parasitology"],["dc.bibliographiccitation.lastpage","234"],["dc.bibliographiccitation.volume","33"],["dc.contributor.author","Schares, Gereon"],["dc.contributor.author","Meyer, J."],["dc.contributor.author","Barwald, A."],["dc.contributor.author","Conraths, Franz J."],["dc.contributor.author","Riebe, R."],["dc.contributor.author","Bohne, Wolfgang"],["dc.contributor.author","Rohn, K."],["dc.contributor.author","Peters, M."],["dc.date.accessioned","2018-11-07T10:40:24Z"],["dc.date.available","2018-11-07T10:40:24Z"],["dc.date.issued","2003"],["dc.description.abstract","Tissue cysts of parasites of the genus Hammondia are rarely described in naturally or experimentally infected intermediate hosts, However, ultrastructural examinations on tissue cyst stages of Hammondia sp. are needed, e.g. to compare these stages with those of Neospora caninum and other related parasites. We describe a cell culture system employed to examine the in vitro development of tissue cysts of a Hammondia sp.-like parasite (isolate FOX 2000/1) which uses the European fox as a definitive host. Cells of a diploid finite cell line from embryonal bovine heart (KH-R CCLV, RIE 090) were infected by inoculation of sporozoites und cultivated for up to 3 months. Transmission electron microscopic examination of 17 day old cell culture material revealed the presence of cyst walls. Infected cell cultures cultivated for 2 months were used to feed a fox. Six to 13 days post infection the fox shed large number,, (n = 1.2 X 10(7)) of Hammondia-sp. like oocysts which could not be distinguished from those used to infect the Cell Culture as determined by DNA sequencing of the internal transcribed spacer I and the D2/D3 domain of the large subunit ribosomal DNA, To find out the proportion of parasitophorous vacuoles that had developed into tissue cysts. the expression of bradyzoite markers was examined by probing infected cell cultures with Mouse polyclonal antibodies against Toxoplasma gondii bradyzoitc antigen I (anti-BAG1) and rat monoclonal antibodies against a cyst wall protein (mAbCC2). Nineteen and 90 days post infection all parasitophorous vacuoles in the cell cultures were positive with anti-BAG1 and mAbCC2. This shows that biologically viable (i.e. infectious) tissue cysts of a fox-derived Hammondia sp. isolate (FOX 2000/1) can be efficiently produced in this cell culture system. Since in Nitro cystogenesis of dog-derived Hammondia heydorni has not been observed et, in vitro cyst formation might be one trait to separate fox-derived Hammondia sp. from H. heydorni on a species level. (C) 2003 Australian Society for Parasitology Inc. Published by Elsevier Science Ltd. All rights reserved."],["dc.identifier.doi","10.1016/S0020-7519(03)00009-2"],["dc.identifier.isi","000182359100001"],["dc.identifier.pmid","12670509"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/46295"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Pergamon-elsevier Science Ltd"],["dc.relation.issn","0020-7519"],["dc.title","A Hammondia-like parasite from the European fox (Vulpes vulpes) forms biologically viable tissue cysts in cell culture"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","835"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","International Journal for Parasitology"],["dc.bibliographiccitation.lastpage","841"],["dc.bibliographiccitation.volume","41"],["dc.contributor.author","Lin, San San"],["dc.contributor.author","Blume, Martin"],["dc.contributor.author","von Ahsen, Nicolas"],["dc.contributor.author","Gross, Uwe"],["dc.contributor.author","Bohne, Wolfgang"],["dc.date.accessioned","2018-11-07T08:54:48Z"],["dc.date.available","2018-11-07T08:54:48Z"],["dc.date.issued","2011"],["dc.description.abstract","Apicomplexan parasites undergo metabolic shifts in adaptation to environmental changes. Here, we investigate the metabolic requirements which are responsible for ATP homeostasis in the extracellular stage of Toxoplasma gondii. Surprisingly, we found that freshly released tachyzoites are able to maintain a constant ATP level during the first hour of extracellular incubation without the acquisition of external carbon sources. We further demonstrated that the extent of gliding motility and that of host cell invasion is independent from the availability of external carbon sources during this one hour extracellular period. The ATP level and the invasion efficiency of extracellular parasites were severely decreased by treatment with the glycolysis inhibitor, 2-deoxy-D-glucose, but not by the F(0)F(1)-ATPase inhibitor, oligomycin. This suggests that although the uptake of glucose itself is not required during the 1 h incubation period, extracellular parasites depend on the activity of the glycolytic pathway for ATP homeostasis. Furthermore, active glycolysis was evident by the secretion of lactate into the culture medium, even in the absence of external carbon sources. Together, our studies suggest that tachyzoites are independent from external carbon sources within the first hour of their extracellular life, which is the most relevant time span for finding a new host cell, but rely on the glycolytic metabolisation of internal carbon sources for ATP maintenance, gliding motility and host cell invasion. (C) 2011 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved."],["dc.identifier.doi","10.1016/j.ijpara.2011.03.005"],["dc.identifier.isi","000292234700004"],["dc.identifier.pmid","21515276"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/22753"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Sci Ltd"],["dc.relation.issn","0020-7519"],["dc.title","Extracellular Toxoplasma gondii tachyzoites do not require carbon source uptake for ATP maintenance, gliding motility and invasion in the first hour of their extracellular life"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","International Journal of Medical Microbiology"],["dc.bibliographiccitation.volume","296"],["dc.contributor.author","Ronnebaumer, K."],["dc.contributor.author","Gross, U."],["dc.contributor.author","Bohne, Wolfgang"],["dc.date.accessioned","2018-11-07T09:19:21Z"],["dc.date.available","2018-11-07T09:19:21Z"],["dc.date.issued","2006"],["dc.format.extent","73"],["dc.identifier.isi","000241442600060"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/28614"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Gmbh, Urban & Fischer Verlag"],["dc.publisher.place","Jena"],["dc.relation.conference","58th Annual Conference of the German-Society-of-Hygiene-and-Microbiology"],["dc.relation.eventlocation","Wurzburg, GERMANY"],["dc.relation.issn","1438-4221"],["dc.title","Nutrient acquisition in Encephalitozoon cuniculi- a microsporidian with extreme reduction of biosynthetic pathways"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","720"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Microorganisms"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Schwanbeck, Julian"],["dc.contributor.author","Bohne, Wolfgang"],["dc.contributor.author","Hasdemir, Ufuk"],["dc.contributor.author","Groß, Uwe"],["dc.contributor.author","Pfeifer, Yvonne"],["dc.contributor.author","Bunk, Boyke"],["dc.contributor.author","Riedel, Thomas"],["dc.contributor.author","Spröer, Cathrin"],["dc.contributor.author","Overmann, Jörg"],["dc.contributor.author","Zautner, Andreas E."],["dc.contributor.author","Frickmann, Hagen"],["dc.date.accessioned","2021-06-01T09:42:39Z"],["dc.date.available","2021-06-01T09:42:39Z"],["dc.date.issued","2021"],["dc.description.abstract","Mobile genetic elements, such as plasmids, facilitate the spread of antibiotic resistance genes in Enterobacterales. In line with this, we investigated the plasmid-resistome of seven blaOXA-48 gene-carrying Klebsiella pneumoniae isolates, which were isolated between 2013 and 2014 at the University Medical Center in Göttingen, Germany. All isolates were subjected to complete genome sequencing including the reconstruction of entire plasmid sequences. In addition, phenotypic resistance testing was conducted. The seven isolates comprised both disease-associated isolates and colonizers isolated from five patients. They fell into two clusters of three sequence type (ST)101 and two ST11 isolates, respectively; and ST15 and ST23 singletons. The seven isolates harbored various plasmids of the incompatibility (Inc) groups IncF, IncL/M, IncN, IncR, and a novel plasmid chimera. All blaOXA-48 genes were encoded on the IncL/M plasmids. Of note, distinct phenotypical resistance patterns associated with different sets of resistance genes encoded by IncL/M and IncR plasmids were observed among isolates of the ST101 cluster in spite of high phylogenetic relatedness of the bacterial chromosomes, suggesting nosocomial transmission. This highlights the importance of plasmid uptake and plasmid recombination events for the fast generation of resistance variability after clonal transmission. In conclusion, this study contributes a piece in the puzzle of molecular epidemiology of resistance gene-carrying plasmids in K. pneumoniae in Germany."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2021"],["dc.identifier.doi","10.3390/microorganisms9040720"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/85312"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.eissn","2076-2607"],["dc.relation.orgunit","Institut für Medizinische Mikrobiologie"],["dc.rights","CC BY 4.0"],["dc.title","Detection of a New Resistance-Mediating Plasmid Chimera in a blaOXA-48-Positive Klebsiella pneumoniae Strain at a German University Hospital"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","PII S0020-7519(02)00126-1"],["dc.bibliographiccitation.firstpage","1253"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","International Journal for Parasitology"],["dc.bibliographiccitation.lastpage","1265"],["dc.bibliographiccitation.volume","32"],["dc.contributor.author","Vonlaufen, N."],["dc.contributor.author","Mueller, N."],["dc.contributor.author","Keller, Nadine"],["dc.contributor.author","Naguleswaran, A."],["dc.contributor.author","Bohne, Wolfgang"],["dc.contributor.author","McAllister, M. M."],["dc.contributor.author","Bjorkman, C."],["dc.contributor.author","Mueller, E."],["dc.contributor.author","Caldelari, R."],["dc.contributor.author","Hemphill, A."],["dc.date.accessioned","2018-11-07T10:05:34Z"],["dc.date.available","2018-11-07T10:05:34Z"],["dc.date.issued","2002"],["dc.description.abstract","Neospora caninum, like Toxoplasma gondii, undergoes stage conversion in chronically infected animals, and forms tissue cysts which contain the slowly proliferating bradyzoite stage. These tissue cysts are delineated by a cyst wall, protect the parasite from physiological and immunological reactions on part of the host, and bradyzoites remain viable within an infected host for many years. However, unlike T gondii, N. caninum bradyzoites have been difficult to obtain using in vitro culture techniques, and current protocols, based on those developed for T. gondii, have been shown to be not very efficient in promoting tachyzoite-to-bradyzoite stage conversion. We report here an alternative in vitro culture method to obtain stage conversion of N. caninum from the proliferative to the cystic stage by using the Nc-Liverpool isolate, murine epidermal keratinocytes as host cells, and continuous treatment of infected cultures with 70 muM sodium nitroprusside for up to 8 days. This treatment significantly reduced parasite proliferation as assessed by Neospora-specific quantitative real-lime PCR. The expression of bradyzoite markers was analysed by immunofluorescence following 4 and 8 days of in vitro culture using antibodies directed against bradyzoite antigen 1, the mAbCC2, and the lectin Dolichos biflorus agglutinin. Expression of the tachyzoite-specific immunodominant antigen NcSAG1 and the tachyzoite antigen NcMIC1 was also assessed. Transmission electron microscopy revealed that the majority of parasitophorous vacuoles were in the process of forming a distinct cyst wall through accumulation of granular material at the periphery of the vacuole, and parasites exhibited the typical features of bradyzoites. These findings demonstrate the usefulness of this culture technique as a promising way to study tachyzoite-to-bradyzoite stage conversion in N. caninum in vitro. (C) 2002 Australian Society for Parasitology Inc. Published by Elsevier Science Ltd. All rights reserved."],["dc.identifier.doi","10.1016/S0020-7519(02)00126-1"],["dc.identifier.isi","000178990100007"],["dc.identifier.pmid","12204225"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/38920"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.issn","0020-7519"],["dc.title","Exogenous nitric oxide triggers Neospora caninum tachyzoite-to-bradyzoite stage conversion in murine epidermal keratinocyte cell cultures"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","2087"],["dc.bibliographiccitation.journal","Frontiers in Microbiology"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Emele, Matthias F."],["dc.contributor.author","Joppe, Felix M."],["dc.contributor.author","Riedel, Thomas"],["dc.contributor.author","Overmann, Jörg"],["dc.contributor.author","Rupnik, Maja"],["dc.contributor.author","Cooper, Paul"],["dc.contributor.author","Kusumawati, R. Lia"],["dc.contributor.author","Berger, Fabian K."],["dc.contributor.author","Laukien, Friederike"],["dc.contributor.author","Zimmermann, Ortrud"],["dc.contributor.author","Bohne, Wolfgang"],["dc.contributor.author","Groß, Uwe"],["dc.contributor.author","Bader, Oliver"],["dc.contributor.author","Zautner, Andreas E."],["dc.date.accessioned","2019-09-24T08:07:22Z"],["dc.date.available","2019-09-24T08:07:22Z"],["dc.date.issued","2019"],["dc.description.abstract","Clostridioides difficile, a Gram-positive spore-forming bacterium, is the leading cause of nosocomial diarrhea worldwide and therefore a substantial burden to the healthcare system. During the past decade, hypervirulent PCR-ribotypes (RT) e.g., RT027 or RT176 emerged rapidly all over the world, associated with both, increased severity and mortality rates. It is thus of great importance to identify epidemic strains such as RT027 and RT176 as fast as possible. While commonly used diagnostic methods, e.g., multilocus sequence typing (MLST) or PCR-ribotyping, are time-consuming, proteotyping offers a fast, inexpensive, and reliable alternative solution. In this study, we established a MALDI-TOF-based typing scheme for C. difficile. A total of 109 ribotyped strains representative for five MLST clades were analyzed by MALDI-TOF. MLST, based on whole genome sequences, and PCR-ribotyping were used as reference methods. Isoforms of MS-detectable biomarkers, typically ribosomal proteins, were related with the deduced amino acid sequences and added to the C. difficile proteotyping scheme. In total, we were able to associate nine biomarkers with their encoding genes and include them in our proteotyping scheme. The discriminatory capacity of the C. difficile proteotyping scheme was mainly based on isoforms of L28-M (2 main isoforms), L35-M (4 main isoforms), and S20-M (2 main isoforms) giving rise to at least 16 proteotyping-derived types. In our test population, five of these 16 proteotyping-derived types were detected. These five proteotyping-derived types did not correspond exactly to the included five MLST-based C. difficile clades, nevertheless the subtyping depth of both methods was equivalent. Most importantly, proteotyping-derived clade B contained only isolates of the hypervirulent RT027 and RT176. Proteotyping is a stable and easy-to-perform intraspecies typing method and a promising alternative to currently used molecular techniques. It is possible to distinguish the group of RT027 and RT176 isolates from non-RT027/non-RT176 isolates using proteotyping, providing a valuable diagnostic tool."],["dc.identifier.doi","10.3389/fmicb.2019.02087"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16398"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/62451"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1664-302X"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Proteotyping of Clostridioides difficile as Alternate Typing Method to Ribotyping Is Able to Distinguish the Ribotypes RT027 and RT176 From Other Ribotypes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","6"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Journal of Antimicrobial Chemotherapy"],["dc.bibliographiccitation.lastpage","10"],["dc.bibliographiccitation.volume","74"],["dc.contributor.author","Schwanbeck, Julian"],["dc.contributor.author","Riedel, Thomas"],["dc.contributor.author","Laukien, Friederike"],["dc.contributor.author","Schober, Isabel"],["dc.contributor.author","Oehmig, Ines"],["dc.contributor.author","Zimmermann, Ortrud"],["dc.contributor.author","Overmann, Jörg"],["dc.contributor.author","Groß, Uwe"],["dc.contributor.author","Zautner, Andreas E."],["dc.contributor.author","Bohne, Wolfgang"],["dc.date.accessioned","2020-08-06T06:17:51Z"],["dc.date.available","2020-08-06T06:17:51Z"],["dc.date.issued","2019"],["dc.description.abstract","The identification and characterization of clinical Clostridioides difficile isolates with reduced fidaxomicin susceptibility."],["dc.identifier.doi","10.1093/jac/dky375"],["dc.identifier.pmid","30247587"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/67529"],["dc.language.iso","en"],["dc.relation.eissn","1460-2091"],["dc.relation.issn","0305-7453"],["dc.title","Characterization of a clinical Clostridioides difficile isolate with markedly reduced fidaxomicin susceptibility and a V1143D mutation in rpoB"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]