Prpic-Schäper, Nikola-Michael

[["dc.bibliographiccitation.firstpage","399"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Development Genes and Evolution"],["dc.bibliographiccitation.lastpage","407"],["dc.bibliographiccitation.volume","219"],["dc.contributor.author","Kittelmann, Maike"],["dc.contributor.author","Schinko, Johannes B."],["dc.contributor.author","Winkler, Marco"],["dc.contributor.author","Bucher, Gregor"],["dc.contributor.author","Wimmer, Ernst A."],["dc.contributor.author","Prpic, Nikola-Michael"],["dc.date.accessioned","2018-11-07T11:25:55Z"],["dc.date.available","2018-11-07T11:25:55Z"],["dc.date.issued","2009"],["dc.description.abstract","The genetic control of leg development is well characterized in the fly Drosophila melanogaster. These control mechanisms, however, must differ to some degree between different insect species to account for the morphological diversity of thoracic legs in the insects. The legs of the flour beetle Tribolium castaneum differ from the Drosophila legs in their developmental mode as well as in their specific morphology especially at the larval stage. In order to identify genes involved in the morphogenesis of the Tribolium larval legs, we have analyzed EGFP enhancer trap lines of Tribolium. We have identified the zfh2 gene as a novel factor required for normal leg development in Tribolium. RNA interference with zfh2 function leads to two alternative classes of leg phenotype. The loss of a leg segment boundary and the generation of ectopic outgrowths in one class of phenotype suggest a role in leg segmentation and segment growth. The malformation of the pretarsal claw in the second class of phenotype suggests a role in distal development and the morphogenesis of the claw-shaped morphology of the pretarsus. This suggests that zfh2 is involved in the regulation of an unidentified target gene in a concentration-dependent manner. Our results demonstrate that enhancer trap screens in T. castaneum have the potential to identify novel gene functions regulating specific developmental processes."],["dc.identifier.doi","10.1007/s00427-009-0303-y"],["dc.identifier.isi","000271397600002"],["dc.identifier.pmid","19760181"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?goescholar/3758"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/56738"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","0949-944X"],["dc.rights.access","openAccess"],["dc.subject.ddc","570"],["dc.title","Insertional mutagenesis screening identifies the zinc finger homeodomain 2 (zfh2) gene as a novel factor required for embryonic leg development in Tribolium castaneum"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","6945"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Quade, Felix Simon Christian"],["dc.contributor.author","Holtzheimer, Jana"],["dc.contributor.author","Frohn, Jasper"],["dc.contributor.author","Töpperwien, Mareike"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","Prpic, Nikola Michael"],["dc.date.accessioned","2019-07-09T11:51:39Z"],["dc.date.available","2019-07-09T11:51:39Z"],["dc.date.issued","2019"],["dc.description.abstract","Spiders have evolved a unique male copulatory organ, the pedipalp bulb. The morphology of the bulb is species specific and plays an important role in species recognition and prezygotic reproductive isolation. Despite its importance for spider biodiversity, the mechanisms that control bulb development are virtually unknown. We have used confocal laser scanning microscopy (CLSM) and diffusible iodine-based contrast-enhanced micro computed tomography (dice-µCT) to study bulb development in the spider Parasteatoda tepidariorum. These imaging technologies enabled us to study bulb development in situ, without the use of destructive procedures for the first time. We show here that the inflated pedipalp tip in the subadult stage is filled with haemolymph that rapidly coagulates. Coagulation indicates histolytic processes that disintegrate tibia and tarsus, similar to histolytic processes during metamorphosis in holometabolous insects. The coagulated material contains cell inclusions that likely represent the cell source for the re-establishment of tarsus and tibia after histolysis, comparable to the histoblasts in insect metamorphosis. The shape of the coagulated mass prefigures the shape of the adult tarsus (cymbium) like a blueprint for the histoblasts. This suggests a unique role for controlled coagulation after histolysis in the metamorphosis-like morphogenesis of the male pedipalp."],["dc.identifier.doi","10.1038/s41598-019-43192-9"],["dc.identifier.pmid","31061504"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16163"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59982"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.notes.intern","Merged from goescholar"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.orgunit","Fakultät für Physik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0/"],["dc.subject.ddc","570"],["dc.subject.gro","biomedical tomography"],["dc.title","Formation and development of the male copulatory organ in the spider Parasteatoda tepidariorum involves a metamorphosis-like process"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]