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Priebe, Marius
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Priebe, Marius
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Priebe, Marius
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Priebe, M.
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2014Journal Article Research Paper [["dc.bibliographiccitation.artnumber","088102"],["dc.bibliographiccitation.firstpage","3"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Physical Review Letters"],["dc.bibliographiccitation.lastpage","7"],["dc.bibliographiccitation.volume","112"],["dc.contributor.author","Weinhausen, Britta"],["dc.contributor.author","Saldanha, Oliva"],["dc.contributor.author","Wilke, Robin N."],["dc.contributor.author","Dammann, Christian"],["dc.contributor.author","Priebe, Marius"],["dc.contributor.author","Burghammer, Manfred"],["dc.contributor.author","Sprung, Michael"],["dc.contributor.author","Köster, Sarah"],["dc.date.accessioned","2017-09-07T11:46:29Z"],["dc.date.available","2017-09-07T11:46:29Z"],["dc.date.issued","2014"],["dc.description.abstract","High-resolution x-ray imaging techniques offer a variety of possibilities for studying the nanoscale structure of biological cells. A challenging task remains the study of cells by x rays in their natural, aqueous environment. Here, we overcome this limitation by presenting scanning x-ray diffraction measurements with beam sizes in the range of a few hundred nm on living and fixed-hydrated eukaryotic cells in microfluidic devices which mimic a native environment. The direct comparison between fixed-hydrated and living cells shows distinct differences in the scattering signal, pointing to structural changes on the order of 30 to 50 nm."],["dc.identifier.doi","10.1103/PhysRevLett.112.088102"],["dc.identifier.gro","3142182"],["dc.identifier.isi","000331957600012"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/5443"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1079-7114"],["dc.relation.issn","0031-9007"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Köster (Cellular Biophysics)"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","x-ray scattering"],["dc.subject.gro","cellular biophysics"],["dc.subject.gro","microfluidics"],["dc.title","Scanning X-Ray Nanodiffraction on Living Eukaryotic Cells in Microfluidic Environments"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]Details DOI WOS2010Journal Article Research Paper [["dc.bibliographiccitation.artnumber","043056"],["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","New Journal of Physics"],["dc.bibliographiccitation.lastpage","13"],["dc.bibliographiccitation.volume","12"],["dc.contributor.affiliation","Priebe, M;"],["dc.contributor.affiliation","Kalbfleisch, S;"],["dc.contributor.affiliation","Tolkiehn, M;"],["dc.contributor.affiliation","Köster, S;"],["dc.contributor.affiliation","Abel, B;"],["dc.contributor.affiliation","Davies, R J;"],["dc.contributor.affiliation","Salditt, T;"],["dc.contributor.author","Kalbfleisch, S"],["dc.contributor.author","Tolkiehn, M"],["dc.contributor.author","Köster, Sarah"],["dc.contributor.author","Davies, R J"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","Priebe, Marius"],["dc.contributor.author","Abel, Bernd"],["dc.date.accessioned","2022-10-11T05:21:38Z"],["dc.date.available","2022-10-11T05:21:38Z"],["dc.date.issued","2010"],["dc.date.updated","2022-02-09T22:15:33Z"],["dc.description.abstract","We have investigated multilamellar lipid assemblies in a microfluidic jet, operating at high shear rates of the order of 10(7) s(-1). Compared to classical Couette cells or rheometers, the shear rate was increased by at least 2-3 orders of magnitude, and the sample volume was scaled down correspondingly. At the same time, the jet is characterized by high extensional stress due to elongational flow. A focused synchrotron x-ray beam was used to measure the structure and orientation of the lipid assemblies in the jet. The diffraction patterns indicate conventional multilamellar phases, aligned with the membrane normals oriented along the velocity gradient of the jet. The results indicate that the setup may be well suited for coherent diffractive imaging of oriented biomolecular assemblies and macromolecules at the future x-ray free electron laser (XFEL) sources."],["dc.identifier.doi","10.1088/1367-2630/12/4/043056"],["dc.identifier.eissn","1367-2630"],["dc.identifier.fs","569048"],["dc.identifier.gro","3142934"],["dc.identifier.isi","000277355200005"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/6683"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/116225"],["dc.identifier.url","https://publications.goettingen-research-online.de/handle/2/393"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1367-2630"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.orgunit","Fakultät für Physik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.relation.workinggroup","RG Köster (Cellular Biophysics)"],["dc.rights","Goescholar"],["dc.rights.uri","https://publishingsupport.iopscience.iop.org/open_access/"],["dc.subject.gro","molecular biophysics"],["dc.subject.gro","microfluidics"],["dc.title","Orientation of biomolecular assemblies in a microfluidic jet"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]Details DOI WOS