Priebe, Marius

[["dc.bibliographiccitation.artnumber","088102"],["dc.bibliographiccitation.firstpage","3"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Physical Review Letters"],["dc.bibliographiccitation.lastpage","7"],["dc.bibliographiccitation.volume","112"],["dc.contributor.author","Weinhausen, Britta"],["dc.contributor.author","Saldanha, Oliva"],["dc.contributor.author","Wilke, Robin N."],["dc.contributor.author","Dammann, Christian"],["dc.contributor.author","Priebe, Marius"],["dc.contributor.author","Burghammer, Manfred"],["dc.contributor.author","Sprung, Michael"],["dc.contributor.author","Köster, Sarah"],["dc.date.accessioned","2017-09-07T11:46:29Z"],["dc.date.available","2017-09-07T11:46:29Z"],["dc.date.issued","2014"],["dc.description.abstract","High-resolution x-ray imaging techniques offer a variety of possibilities for studying the nanoscale structure of biological cells. A challenging task remains the study of cells by x rays in their natural, aqueous environment. Here, we overcome this limitation by presenting scanning x-ray diffraction measurements with beam sizes in the range of a few hundred nm on living and fixed-hydrated eukaryotic cells in microfluidic devices which mimic a native environment. The direct comparison between fixed-hydrated and living cells shows distinct differences in the scattering signal, pointing to structural changes on the order of 30 to 50 nm."],["dc.identifier.doi","10.1103/PhysRevLett.112.088102"],["dc.identifier.gro","3142182"],["dc.identifier.isi","000331957600012"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/5443"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1079-7114"],["dc.relation.issn","0031-9007"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Köster (Cellular Biophysics)"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","x-ray scattering"],["dc.subject.gro","cellular biophysics"],["dc.subject.gro","microfluidics"],["dc.title","Scanning X-Ray Nanodiffraction on Living Eukaryotic Cells in Microfluidic Environments"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","680"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","690"],["dc.bibliographiccitation.volume","110"],["dc.contributor.author","Bernhardt, Marten"],["dc.contributor.author","Priebe, Marius"],["dc.contributor.author","Osterhoff, Markus"],["dc.contributor.author","Wollnik, Bernd"],["dc.contributor.author","Diaz, Ana"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","Rehfeldt, Florian"],["dc.date.accessioned","2017-09-07T11:54:39Z"],["dc.date.available","2017-09-07T11:54:39Z"],["dc.date.issued","2016"],["dc.description.abstract","Adult human mesenchymal stem cells show structural rearrangements of their cytoskeletal network during mechanically induced differentiation toward various cell types. In particular, the alignment of acto-myosin fibers is cell fate-dependent and can serve as an early morphological marker of differentiation. Quantification of such nanostructures on a mesoscopic scale requires high-resolution imaging techniques. Here, we use small-angle x-ray scattering with a spot size in the micro- and submicrometer range as a high-resolution and label-free imaging technique to reveal structural details of stem cells and differentiated cell types. We include principal component analysis into an automated empirical analysis scheme that allows the local characterization of oriented structures. Results on freeze-dried samples lead to quantitative structural information for all cell lines tested: differentiated cells reveal pronounced structural orientation and a relatively intense overall diffraction signal, whereas naive human mesenchymal stem cells lack these features. Our data support the hypothesis of stem cells establishing ordered structures along their differentiation process."],["dc.identifier.doi","10.1016/j.bpj.2015.12.017"],["dc.identifier.gro","3141731"],["dc.identifier.isi","000369467800017"],["dc.identifier.pmid","26840732"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14077"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/446"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1542-0086"],["dc.relation.issn","0006-3495"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.orgunit","Fakultät für Physik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights","CC BY 4.0"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","x-ray scattering"],["dc.title","X-Ray Micro- and Nanodiffraction Imaging on Human Mesenchymal Stem Cells and Differentiated Cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","10"],["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Optical Nanoscopy"],["dc.bibliographiccitation.lastpage","7"],["dc.bibliographiccitation.volume","1"],["dc.contributor.author","Bartels, Matthias"],["dc.contributor.author","Priebe, Marius"],["dc.contributor.author","Wilke, Robin N."],["dc.contributor.author","Krüger, Sven P"],["dc.contributor.author","Giewekemeyer, Klaus"],["dc.contributor.author","Kalbfleisch, Sebastian"],["dc.contributor.author","Olendrowitz, Christian"],["dc.contributor.author","Sprung, Michael"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2017-09-07T11:54:07Z"],["dc.date.available","2017-09-07T11:54:07Z"],["dc.date.issued","2012"],["dc.description.abstract","We have imaged the three-dimensional density distribution of unstained and unsliced, freeze-dried cells of the gram-positive bacterium Deinococcus radiodurans by tomographic x-ray propagation microscopy, i.e. projection tomography with phase contrast formation by free space propagation. The work extends previous x-ray imaging of biological cells in the simple in-line holography geometry to full three-dimensional reconstruction, based on a fast iterative phase reconstruction algorithm which circumvents the usual twin-image problem. The sample is illuminated by the highly curved wave fronts emitted from a virtual quasi-point source with 10 nm cross section, realized by two crossed x-ray waveguides. The experimental scheme allows for a particularly dose efficient determination of the 3D density distribution in the cellular structure."],["dc.identifier.doi","10.1186/2192-2853-1-10"],["dc.identifier.fs","593648"],["dc.identifier.gro","3145116"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9581"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2817"],["dc.language.iso","en"],["dc.notes","Funding by the DFG collaborative research center SFB 755\r\nNanoscale Photonic Imaging and the German Ministry of Education and\r\nResearch (Grant No. 05K10MGA) is gratefully acknowledged."],["dc.notes.intern","Crossref Import"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.relation.issn","2192-2853"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.orgunit","Fakultät für Physik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","biomedical tomography"],["dc.title","Low-dose three-dimensional hard x-ray imaging of bacterial cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","no"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","867"],["dc.bibliographiccitation.journal","Journal of Synchrotron Radiation"],["dc.bibliographiccitation.lastpage","878"],["dc.bibliographiccitation.volume","22"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","Osterhoff, Markus"],["dc.contributor.author","Krenkel, Martin"],["dc.contributor.author","Wilke, Robin N."],["dc.contributor.author","Priebe, Marius"],["dc.contributor.author","Bartels, Matthias"],["dc.contributor.author","Kalbfleisch, Sebastian"],["dc.contributor.author","Sprung, Michael"],["dc.date.accessioned","2017-09-07T11:43:44Z"],["dc.date.available","2017-09-07T11:43:44Z"],["dc.date.issued","2015"],["dc.description.abstract","A compound optical system for coherent focusing and imaging at the nanoscale is reported, realised by high-gain fixed-curvature elliptical mirrors in combination with X-ray waveguide optics or different cleaning apertures. The key optical concepts are illustrated, as implemented at the Gottingen Instrument for Nano-Imaging with X-rays (GINIX), installed at the P10 coherence beamline of the PETRA III storage ring at DESY, Hamburg, and examples for typical applications in biological imaging are given. Characteristic beam configurations with the recently achieved values are also described, meeting the different requirements of the applications, such as spot size, coherence or bandwidth. The emphasis of this work is on the different beam shaping, filtering and characterization methods."],["dc.identifier.doi","10.1107/S1600577515007742"],["dc.identifier.gro","3141875"],["dc.identifier.isi","000357407900001"],["dc.identifier.pmid","26134789"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2045"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation","SFB 755: Nanoscale Photonic Imaging"],["dc.relation.eissn","1600-5775"],["dc.relation.issn","0909-0495"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray optics"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","x-ray scattering"],["dc.title","Compound focusing mirror and X-ray waveguide optics for coherent imaging and nano-diffraction"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","19232"],["dc.bibliographiccitation.issue","17"],["dc.bibliographiccitation.journal","Optics Express"],["dc.bibliographiccitation.lastpage","19254"],["dc.bibliographiccitation.volume","20"],["dc.contributor.author","Wilke, Robin N."],["dc.contributor.author","Priebe, Marius"],["dc.contributor.author","Bartels, Matthias"],["dc.contributor.author","Giewekemeyer, Klaus"],["dc.contributor.author","Diaz, Ana"],["dc.contributor.author","Karvinen, P."],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2017-09-07T11:48:27Z"],["dc.date.available","2017-09-07T11:48:27Z"],["dc.date.issued","2012"],["dc.description.abstract","Ptychographic coherent X-ray diffractive imaging (PCDI) has been combined with nano-focus X-ray diffraction to study the structure and density distribution of unstained and unsliced bacterial cells, using a hard X-ray beam of 6.2keV photon energy, focused to about 90nm by a Fresnel zone plate lens. While PCDI provides images of the bacteria with quantitative contrast in real space with a resolution well below the beam size at the sample, spatially resolved small angle X-ray scattering using the same Fresnel zone plate (cellular nano-diffraction) provides structural information at highest resolution in reciprocal space up to 2nm(-1). We show how the real and reciprocal space approach can be used synergistically on the same sample and with the same setup. In addition, we present 3D hard X-ray imaging of unstained bacterial cells by a combination of ptychography and tomography. (C) 2012 Optical Society of America"],["dc.identifier.doi","10.1364/OE.20.019232"],["dc.identifier.fs","589591"],["dc.identifier.gro","3142482"],["dc.identifier.isi","000307873600075"],["dc.identifier.pmid","23038565"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9566"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/8773"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: Deutsche Forschungsgemeinschaft [SFB 755]"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1094-4087"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.orgunit","Fakultät für Physik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","x-ray scattering"],["dc.title","Hard X-ray imaging of bacterial cells: nano-diffraction and ptychographic reconstruction"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]