Brückner, Bastian Rouven

[["dc.bibliographiccitation.firstpage","6329"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Nano Letters"],["dc.bibliographiccitation.lastpage","6335"],["dc.bibliographiccitation.volume","20"],["dc.contributor.author","Hubrich, Hanna"],["dc.contributor.author","Mey, Ingo P."],["dc.contributor.author","Brückner, Bastian R."],["dc.contributor.author","Mühlenbrock, Peter"],["dc.contributor.author","Nehls, Stefan"],["dc.contributor.author","Grabenhorst, Lennart"],["dc.contributor.author","Oswald, Tabea"],["dc.contributor.author","Steinem, Claudia"],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2020-11-05T15:08:07Z"],["dc.date.available","2020-11-05T15:08:07Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1021/acs.nanolett.0c01769"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/68468"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-352.7"],["dc.relation.eissn","1530-6992"],["dc.relation.issn","1530-6984"],["dc.title","Viscoelasticity of Native and Artificial Actin Cortices Assessed by Nanoindentation Experiments"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","712"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Biochimica et Biophysica Acta (BBA) - Molecular Cell Research"],["dc.bibliographiccitation.lastpage","722"],["dc.bibliographiccitation.volume","1833"],["dc.contributor.author","Pietuch, Anna"],["dc.contributor.author","Brueckner, Bastian Rouven"],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2018-11-07T09:27:47Z"],["dc.date.available","2018-11-07T09:27:47Z"],["dc.date.issued","2013"],["dc.description.abstract","Osmotic stress poses one of the most fundamental challenges to living cells. Particularly, the largely inextensible plasma membrane of eukaryotic cells easily ruptures under in-plane tension calling for sophisticated strategies to readily respond to osmotic stress. We describe how epithelial cells react and adapt mechanically to the exposure to hypotonic and hypertonic solutions in the context of a confluent monolayer. Site-specific indentation experiments in conjunction with tether pulling on individual cells have been carried out with an atomic force microscope to reveal spatio-temporal changes in membrane tension and surface area. We found that cells compensate for an increase in lateral tension due to hypoosmotic stress by sacrificing excess of membrane area stored in protrusions and invaginations such as microvilli and caveolae. At mild hypotonic conditions lateral tension increases partly compensated by surface are regulation, i.e. the cell sacrifices some of its membrane reservoirs. A loss of membrane-actin contacts occurs upon exposure to stronger hypotonic solutions giving rise to a drop in lateral tension. Tension release recovers on longer time scales by an increasing endocytosis, which efficiently removes excess membrane from the apical side to restore the initial pre-stress. Hypertonic solutions lead to shrinkage of cells and collapse of the apical membrane onto the cortex. Exposure to distilled water leads to stiffening of cells due to removal of excess surface area and tension increase due to elevated osmotic pressure across the plasma membrane. (c) 2012 Elsevier B.V. All rights reserved."],["dc.description.sponsorship","DFG [SFB 937]; Dorothea Schlozer Foundation of Georg-August-University"],["dc.identifier.doi","10.1016/j.bbamcr.2012.11.006"],["dc.identifier.isi","000315308100029"],["dc.identifier.pmid","23178740"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/30619"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Bv"],["dc.relation.issn","0167-4889"],["dc.title","Membrane tension homeostasis of epithelial cells through surface area regulation in response to osmotic stress"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","77"],["dc.bibliographiccitation.journal","Progress in Biophysics and Molecular Biology"],["dc.bibliographiccitation.lastpage","90"],["dc.bibliographiccitation.volume","144"],["dc.contributor.author","Brückner, Bastian Rouven"],["dc.contributor.author","Nöding, Helen"],["dc.contributor.author","Skamrahl, Mark"],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2020-12-10T15:20:42Z"],["dc.date.available","2020-12-10T15:20:42Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1016/j.pbiomolbio.2018.08.010"],["dc.identifier.issn","0079-6107"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/72768"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Mechanical and morphological response of confluent epithelial cell layers to reinforcement and dissolution of the F-actin cytoskeleton"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3320"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Nano Letters"],["dc.bibliographiccitation.lastpage","3326"],["dc.bibliographiccitation.volume","17"],["dc.contributor.author","Baronsky, Thilo"],["dc.contributor.author","Ruhlandt, Daja"],["dc.contributor.author","Brückner, Bastian Rouven"],["dc.contributor.author","Schäfer, Jonas"],["dc.contributor.author","Karedla, Narain"],["dc.contributor.author","Isbaner, Sebastian"],["dc.contributor.author","Hähnel, Dirk"],["dc.contributor.author","Gregor, Ingo"],["dc.contributor.author","Enderlein, Jörg"],["dc.contributor.author","Janshoff, Andreas"],["dc.contributor.author","Chizhik, Alexey I."],["dc.date.accessioned","2018-04-23T11:48:51Z"],["dc.date.available","2018-04-23T11:48:51Z"],["dc.date.issued","2017"],["dc.description.abstract","The biological process of the epithelial-to-mesenchymal transition (EMT) allows epithelial cells to enhance their migratory and invasive behavior and plays a key role in embryogenesis, fibrosis, wound healing, and metastasis. Among the multiple biochemical changes from an epithelial to a mesenchymal phenotype, the alteration of cellular dynamics in cell–cell as well as cell–substrate contacts is crucial. To determine these variations over the whole time scale of the EMT, we measure the cell–substrate distance of epithelial NMuMG cells during EMT using our newly established metal-induced energy transfer (MIET) microscopy, which allows one to achieve nanometer axial resolution. We show that, in the very first hours of the transition, the cell–substrate distance increases substantially, but later in the process after reaching the mesenchymal state, this distance is reduced again to the level of untreated cells. These findings relate to a change in the number of adhesion points and will help to better understand remodeling processes associated with wound healing, embryonic development, cancer progression, or tissue regeneration."],["dc.identifier.doi","10.1021/acs.nanolett.7b01558"],["dc.identifier.gro","3142100"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/13588"],["dc.language.iso","en"],["dc.notes.intern","lifescience updates Crossref Import"],["dc.notes.status","final"],["dc.relation.issn","1530-6984"],["dc.title","Cell–Substrate Dynamics of the Epithelial-to-Mesenchymal Transition"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Brückner, Bastian Rouven"],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2020-12-10T18:10:12Z"],["dc.date.available","2020-12-10T18:10:12Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1038/s41598-018-32421-2"],["dc.identifier.eissn","2045-2322"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/73885"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Importance of integrity of cell-cell junctions for the mechanics of confluent MDCK II cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","223"],["dc.bibliographiccitation.journal","Beilstein Journal of Nanotechnology"],["dc.bibliographiccitation.lastpage","231"],["dc.bibliographiccitation.volume","6"],["dc.contributor.author","Pietuch, Anna"],["dc.contributor.author","Brueckner, Bastian Rouven"],["dc.contributor.author","Schneider, David"],["dc.contributor.author","Tarantola, Marco"],["dc.contributor.author","Rosman, Christina"],["dc.contributor.author","Soennichsen, Carsten"],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2018-11-07T10:02:05Z"],["dc.date.available","2018-11-07T10:02:05Z"],["dc.date.issued","2015"],["dc.description.abstract","Background: The impact of gold nanoparticles on cell viability has been extensively studied in the past. Size, shape and surface functionalization including opsonization of gold particles ranging from a few nanometers to hundreds of nanometers are among the most crucial parameters that have been focussed on. Cytoxicity of nanomaterial has been assessed by common cytotoxicity assays targeting enzymatic activity such as LDH, MTT and ECIS. So far, however, less attention has been paid to the mechanical parameters of cells exposed to gold particles, which is an important reporter on the cellular response to external stimuli. Results: Mechanical properties of confluent MDCK II cells exposed to gold nanorods as a function of surface functionalization and concentration have been explored by atomic force microscopy and quartz crystal microbalance measurements in combination with fluorescence and dark-field microscopy. Conclusion: We found that cells exposed to CTAB coated gold nanorods display a concentration-dependent stiffening that cannot be explained by the presence of CTAB alone. The stiffening results presumably from endocytosis of particles removing excess membrane area from the cell's surface. Another aspect could be the collapse of the plasma membrane on the actin cortex. Particles coated with PEG do not show a significant change in elastic properties. This observation is consistent with QCM measurements that show a considerable drop in frequency upon administration of CTAB coated rods suggesting an increase in acoustic load corresponding to a larger stiffness (storage modulus)."],["dc.description.sponsorship","DFG"],["dc.identifier.doi","10.3762/bjnano.6.21"],["dc.identifier.isi","000348943900001"],["dc.identifier.pmid","25671166"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/38155"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Beilstein-institut"],["dc.relation.issn","2190-4286"],["dc.title","Mechanical properties of MDCK II cells exposed to gold nanorods"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","9833"],["dc.bibliographiccitation.issue","14"],["dc.bibliographiccitation.journal","Journal of biological chemistry"],["dc.bibliographiccitation.lastpage","9843"],["dc.bibliographiccitation.volume","289"],["dc.contributor.author","Braunger, Julia A."],["dc.contributor.author","Brückner, Bastian R."],["dc.contributor.author","Nehls, Stefan"],["dc.contributor.author","Pietuch, Anna"],["dc.contributor.author","Gerke, Volker"],["dc.contributor.author","Mey, Ingo"],["dc.contributor.author","Janshoff, Andreas"],["dc.contributor.author","Steinem, Claudia"],["dc.date.accessioned","2017-09-07T11:46:19Z"],["dc.date.available","2017-09-07T11:46:19Z"],["dc.date.issued","2014"],["dc.description.abstract","Background: Ezrin can establish a dynamic linkage between plasma membrane and cytoskeleton. Results: The individual bond strength between ezrin and F-actin is small, but the number of attachment sites is significantly altered by phosphatidylinositol 4,5-bisphosphate (PIP2). Conclusion: PIP2 activates ezrin to establish multiple weak ezrin/F-actin interactions. Significance: Plasma membrane tension is maintained by ezrin/F-actin interactions. Direct linkage between the plasma membrane and the actin cytoskeleton is controlled by the protein ezrin, a member of the ezrin-radixin-moesin protein family. To function as a membrane-cytoskeleton linker, ezrin needs to be activated in a process that involves binding of ezrin to phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphorylation of a conserved threonine residue. Here, we used colloidal probe microscopy to quantitatively analyze the interaction between ezrin and F-actin as a function of these activating factors. We show that the measured individual unbinding forces between ezrin and F-actin are independent of the activating parameters, in the range of approximately 50 piconewtons. However, the cumulative adhesion energy greatly increases in the presence of PIP2 demonstrating that a larger number of bonds between ezrin and F-actin has formed. In contrast, the phosphorylation state, represented by phosphor-mimetic mutants of ezrin, only plays a minor role in the activation process. These results are in line with in vivo experiments demonstrating that an increase in PIP2 concentration recruits more ezrin to the apical plasma membrane of polarized cells and significantly increases the membrane tension serving as a measure of the adhesion sites between the plasma membrane and the F-actin network."],["dc.identifier.doi","10.1074/jbc.M113.530659"],["dc.identifier.gro","3142143"],["dc.identifier.isi","000333807000033"],["dc.identifier.pmid","24500715"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/5022"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: Deutsche Forschungsgemeinschaft [STE 884/11-1, GE 514/8-1, GE 514/9-1]"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1083-351X"],["dc.relation.issn","0021-9258"],["dc.title","Phosphatidylinositol 4,5-Bisphosphate Alters the Number of Attachment Sites between Ezrin and Actin Filaments A COLLOIDAL PROBE STUDY "],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","European Biophysics Journal"],["dc.bibliographiccitation.volume","42"],["dc.contributor.author","Brueckner, B. R."],["dc.contributor.author","Pietuch, Anna"],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2018-11-07T09:22:32Z"],["dc.date.available","2018-11-07T09:22:32Z"],["dc.date.issued","2013"],["dc.format.extent","S46"],["dc.identifier.isi","000330215300043"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/29361"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.publisher.place","New york"],["dc.relation.eventlocation","Lisbon, PORTUGAL"],["dc.relation.issn","1432-1017"],["dc.relation.issn","0175-7571"],["dc.title","Challenging cellular mechanics - tension homeostasis by surface area regulation"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","724"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","735"],["dc.bibliographiccitation.volume","112"],["dc.contributor.author","Brueckner, Bastian Rouven"],["dc.contributor.author","Noeding, Helen"],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2018-11-07T10:27:10Z"],["dc.date.available","2018-11-07T10:27:10Z"],["dc.date.issued","2017"],["dc.description.abstract","The local mechanical properties of cells are frequently probed by force indentation experiments carried out with an atomic force microscope. Application of common contact models provides a single parameter, the Young's modulus, to describe the elastic properties of cells. The viscoelastic response of cells, however, is generally measured in separate microrheological experiments that provide complex shear moduli as a function of time or frequency. Here, we present a straightforward way to obtain rheological properties of cells from regular force distance curves collected in typical force indentation measurements. The method allows us to record the stress-strain relationship as well as changes in the weak power law of the viscoelastic moduli. We derive an analytical function based on the elastic-viscoelastic correspondence principle applied to Hertzian contact mechanics to model both indentation and retraction curves. Rheological properties are described by standard viscoelastic models and the paradigmatic weak power law found to interpret the viscoelastic properties of living cells best. We compare our method with atomic force microscopy-based active oscillatory microrheology and show that the method to determine the power law coefficient is robust against drift and largely independent of the indentation depth and indenter geometry. Cells were subject to Cytochalasin D treatment to provoke a drastic change in the power law coefficient and to demonstrate the feasibility of the approach to capture rheological changes extremely fast and precisely. The method is easily adaptable to different indenter geometries and acquires viscoelastic data with high spatiotemporal resolution."],["dc.identifier.doi","10.1016/j.bpj.2016.12.032"],["dc.identifier.isi","000395617800017"],["dc.identifier.pmid","28256232"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/43194"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Cell Press"],["dc.relation.issn","1542-0086"],["dc.relation.issn","0006-3495"],["dc.title","Viscoelastic Properties of Confluent MDCK II Cells Obtained from Force Cycle Experiments"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3075"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Biochimica et Biophysica Acta (BBA) - Molecular Cell Research"],["dc.bibliographiccitation.lastpage","3082"],["dc.bibliographiccitation.volume","1853"],["dc.contributor.author","Bruckner, Bastian R."],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2018-11-07T09:49:44Z"],["dc.date.available","2018-11-07T09:49:44Z"],["dc.date.issued","2015"],["dc.description.abstract","Cellular mechanics plays a crucial role in many biological processes such as cell migration, cell growth, embryogenesis, and oncogenesis. Epithelia respond to environmental cues comprising biochemical and physical stimuli through defined changes in cell elasticity. For instance, cells can differentiate between certain properties such as viscoelasticity or topography of substrates by adapting their own elasticity and shape. A living cell is a complex viscoelastic body that not only exhibits a shell architecture composed of a membrane attached to a cytoskeleton cortex but also generates contractile forces through its actomyosin network. Here we review cellular mechanics of single cells in the context of epithelial cell layers responding to chemical and physical stimuli. This article is part of a Special Issue entitled: Mechanobiology. (C) 2015 Elsevier B.V. All rights reserved."],["dc.identifier.doi","10.1016/j.bbamcr.2015.07.010"],["dc.identifier.isi","000363069200012"],["dc.identifier.pmid","26193077"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/35565"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Bv"],["dc.relation.issn","0006-3002"],["dc.relation.issn","0167-4889"],["dc.title","Elastic properties of epithelial cells probed by atomic force microscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]