Salditt, Tim

[["dc.bibliographiccitation.firstpage","24"],["dc.bibliographiccitation.issue","S2"],["dc.bibliographiccitation.journal","Microscopy and Microanalysis"],["dc.bibliographiccitation.lastpage","25"],["dc.bibliographiccitation.volume","24"],["dc.contributor.author","Töpperwien, Mareike"],["dc.contributor.author","Eckermann, Marina"],["dc.contributor.author","Robisch, Anna Lena"],["dc.contributor.author","Stadelmann, Christine"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2020-03-04T13:40:15Z"],["dc.date.available","2020-03-04T13:40:15Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1017/S1431927618012540"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/63109"],["dc.language.iso","en"],["dc.relation.issn","1431-9276"],["dc.relation.issn","1435-8115"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","biomedical tomography"],["dc.title","3d Virtual Histology of Human Cerebellum by Propagation-Based X-Ray Phase-Contrast Tomography"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2"],["dc.contributor.author","Eckermann, Marina"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.editor","Müller, Bert"],["dc.contributor.editor","Wang, Ge"],["dc.date.accessioned","2022-02-16T14:12:05Z"],["dc.date.available","2022-02-16T14:12:05Z"],["dc.date.issued","2021"],["dc.identifier.doi","10.1117/12.2596133"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/99930"],["dc.relation.conference","SPIE Optical Engineering + Applications"],["dc.relation.eventend","2021-08-05"],["dc.relation.eventlocation","San Diego, California"],["dc.relation.eventstart","2021-08-01"],["dc.relation.isbn","9781510645189"],["dc.relation.isbn","9781510645196"],["dc.relation.ispartof","Developments in X-Ray Tomography XIII"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","biomedical tomography"],["dc.title","Towards correlative imaging of neuronal tissue by phase-contrast x-ray tomography and SEM"],["dc.type","conference_paper"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1261"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Scandinavian Journal of Gastroenterology"],["dc.bibliographiccitation.lastpage","1267"],["dc.bibliographiccitation.volume","55"],["dc.contributor.author","Peruzzi, Niccolò"],["dc.contributor.author","Veress, Béla"],["dc.contributor.author","Dahlin, Lars B."],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","Andersson, Mariam"],["dc.contributor.author","Eckermann, Marina"],["dc.contributor.author","Frohn, Jasper"],["dc.contributor.author","Robisch, Anna-Lena"],["dc.contributor.author","Bech, Martin"],["dc.contributor.author","Ohlsson, Bodil"],["dc.date.accessioned","2021-04-14T08:23:03Z"],["dc.date.available","2021-04-14T08:23:03Z"],["dc.date.issued","2020"],["dc.description.abstract","Objectives: Light microscopical analysis in two dimensions, combined with immunohistochemistry, is\r\npresently the gold standard to describe the enteric nervous system (ENS). Our aim was to assess the\r\nusefulness of three-dimensional (3D) imaging by X-ray phase-contrast tomography in evaluating the\r\nENS of the human bowel.\r\nMaterial and methods: Myenteric ganglia were identified in full-thickness biopsies of the ileum and\r\ncolon by hematoxylin & eosin staining. A1-mm biopsy punch was taken from the paraffin blocks and\r\nplaced into a KaptonVR tube for subsequent tomographic investigation. The samples were scanned,\r\nwithout further preparation, using phase-contrast tomography at two different scales: overview scans\r\n(performed with laboratory setups), which allowed localization of the nervous tissue ( 1mm effective\r\nvoxel size); and high-resolution scans (performed with a synchrotron endstation), which imaged localized\r\nregions of 320x320x320 mm3 (176 nm effective voxel size).\r\nResults: The contrast allowed us to follow the shape and the size changes of the ganglia, as well as\r\nto study their cellular components together with the cells and cellular projections of the periganglional\r\nspace. Furthermore, it was possible to show the 3D network of the myenteric plexus and to quantify\r\nits volume within the samples.\r\nConclusions: Phase-contrast X-ray tomography can be applied for volume analyses of the human ENS\r\nand to study tissue components in unstained paraffin-embedded tissue biopsies. This technique could\r\npotentially be used to study disease mechanisms, and to compare healthy and diseased tissues in clinical\r\nresearch."],["dc.identifier.doi","10.1080/00365521.2020.1815079"],["dc.identifier.pmid","32907418"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/80786"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/70"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","1502-7708"],["dc.relation.issn","0036-5521"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights","CC BY 3.0"],["dc.subject.gro","biomedical tomography"],["dc.title","3D analysis of the myenteric plexus of the human bowel by X-ray phase-contrast tomography – a future method?"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.contributor.author","Eckermann, Marina"],["dc.contributor.author","Frohn, Jasper"],["dc.contributor.author","Reichardt, Marius"],["dc.contributor.author","Osterhoff, Markus"],["dc.contributor.author","Sprung, Michael"],["dc.contributor.author","Westermeier, Fabian"],["dc.contributor.author","Tzankov, Alexandar"],["dc.contributor.author","Werlein, Christopher"],["dc.contributor.author","Kuehnel, Mark"],["dc.contributor.author","Jonigk, Danny"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2020-06-26T11:04:13Z"],["dc.date.available","2020-06-26T11:04:13Z"],["dc.date.issued","2020"],["dc.description.abstract","We present a new approach of three-dimensional (3d) virtual histology and patho-histology based on multi-scale phase contrast x-ray tomography, and use this to investigate the parenchymal-architecture of unstained lung tissue from patients who succumbed to Covid-19. Based on this first proof-of-concept study, we can propose multi-scale phase contrast x-ray tomography as a novel tool to unravel the patho-physiology of Covid-19, extending conventional histology by a third dimension and allowing for a full quantification of tissue remodeling.By combining parallel and cone beam geometry, autopsy samples with a cross section of 4mm are scanned and reconstructed at a resolution and image quality which allows for the segmentation of individual cells. Using the zoom capability of the cone beam geometry, regions-of-interest are reconstructed with a minimum voxel size of 160nm. We exemplify the capability of this approach by 3d visualisation of the diffuse alveolar damage with its prominent hyaline membrane formation, by mapping the 3d distribution and density of lymphocytes infiltrating the tissue, and by providing histograms of characteristic distances from tissue interior to the closest air compartment."],["dc.identifier.doi","10.1101/2020.06.21.20134882"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/66752"],["dc.language.iso","en"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","biomedical tomography"],["dc.subject.gro","other"],["dc.title","3d Virtual Patho-Histology of Lung Tissue from Covid-19 Patients based on Phase Contrast X-ray Tomography"],["dc.type","preprint"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1173"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Journal of Synchrotron Radiation"],["dc.bibliographiccitation.lastpage","1180"],["dc.bibliographiccitation.volume","26"],["dc.contributor.author","Osterhoff, Markus"],["dc.contributor.author","Robisch, Anna-Lena"],["dc.contributor.author","Soltau, Jakob"],["dc.contributor.author","Eckermann, Marina"],["dc.contributor.author","Kalbfleisch, Sebastian"],["dc.contributor.author","Carbone, Dina"],["dc.contributor.author","Johansson, Ulf"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2020-12-10T18:25:59Z"],["dc.date.available","2020-12-10T18:25:59Z"],["dc.date.issued","2019"],["dc.description.abstract","The focusing and coherence properties of the NanoMAX Kirkpatrick–Baez mirror system at the fourth-generation MAX IV synchrotron in Lund have been characterized. The direct measurement of nano-focused X-ray beams is possible by scanning of an X-ray waveguide, serving basically as an ultra-thin slit. In quasi-coherent operation, beam sizes of down to 56 nm (FWHM, horizontal direction) can be achieved. Comparing measured Airy-like fringe patterns with simulations, the degree of coherence"],["dc.identifier.doi","10.1107/S1600577519003886"],["dc.identifier.issn","1600-5775"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16741"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/75900"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.relation.issn","1600-5775"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://goedoc.uni-goettingen.de/licenses"],["dc.subject.gro","x-ray optics"],["dc.title","Focus characterization of the NanoMAX Kirkpatrick–Baez mirror system"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","111130N"],["dc.bibliographiccitation.firstpage","21"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Eckermann, Marina"],["dc.contributor.author","Töpperwien, Mareike"],["dc.contributor.author","Ruhwedel, Torben"],["dc.contributor.author","Möbius, Wiebke"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.editor","Müller-Myhsok, Bertram"],["dc.contributor.editor","Wang, Geng"],["dc.date.accessioned","2020-03-10T15:20:27Z"],["dc.date.available","2020-03-10T15:20:27Z"],["dc.date.issued","2019"],["dc.description.abstract","In the present work, we evaluate and compare the contrast and resolution obtained on different neuronal tissues with propagation-based x-ray phase contrast computed tomography (PB-CT). At our laboratory-based liquid metal-jet setup, we obtain overview datasets at sub-micron resolution of mm3 -sized volumes. In order to evaluate these parameters down to the sub-cellular level, we utilize the synchrotron endstation GINIX at P10, DESY. At this dedicated endstation1 developed and operated by our group, we utilize x-ray waveguide optics for highresolution cone-beam scans at strong geometrical magnification M. Exploiting this multi-scale approach, we investigate the image quality of cerebellum tissue treated by different heavy-metal stains. In addition, we study the electron density contrast in unstained tissues. Different embedding media are utilized depending on the stain, which also significantly affects contrast and image quality. With this work, we want to contribute to an optimized sample preparation to study the neuronal architecture of the brain tissue in greater detail in three dimensions (3d). © (2019) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only."],["dc.identifier.doi","10.1117/12.2528432"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/63286"],["dc.language.iso","en"],["dc.relation.eventend","2019-08-15"],["dc.relation.eventlocation","San Diego, California, United States"],["dc.relation.eventstart","2019-08-11"],["dc.relation.isbn","978-1-5106-2919-6"],["dc.relation.isbn","978-1-5106-2920-2"],["dc.relation.ispartof","Proc. SPIE 11113"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","biomedical tomography"],["dc.title","Evaluation of different heavy-metal stains and embedding media for phase contrast tomography of neuronal tissue"],["dc.type","conference_paper"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3994"],["dc.bibliographiccitation.issue","29"],["dc.bibliographiccitation.journal","World Journal of Gastroenterology"],["dc.bibliographiccitation.lastpage","4006"],["dc.bibliographiccitation.volume","28"],["dc.contributor.author","Veress, Bela"],["dc.contributor.author","Peruzzi, Niccolò"],["dc.contributor.author","Eckermann, Marina"],["dc.contributor.author","Frohn, Jasper"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","Bech, Martin"],["dc.contributor.author","Ohlsson, Bodil"],["dc.date.accessioned","2022-10-04T10:21:43Z"],["dc.date.available","2022-10-04T10:21:43Z"],["dc.date.issued","2022-08-07"],["dc.identifier.doi","10.3748/wjg.v28.i29.3994"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/114485"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-600"],["dc.relation.issn","1007-9327"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.title","Structure of the myenteric plexus in normal and diseased human ileum analyzed by X-ray virtual histology slices"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Biomedical Optics Express"],["dc.contributor.author","Joppe, Karina"],["dc.contributor.author","Nicolas, Jan-David"],["dc.contributor.author","Grünewald, Tilman"],["dc.contributor.author","Eckermann, Marina"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","Lingor, Paul"],["dc.date.accessioned","2020-04-23T12:33:42Z"],["dc.date.available","2020-04-23T12:33:42Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1364/BOE.389408"],["dc.identifier.pmid","33014542"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17773"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/64291"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/45"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","2156-7085"],["dc.relation.issn","2156-7085"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","x-ray scattering"],["dc.title","Elemental quantification and analysis of structural abnormalities in neurons from Parkinson’s-diseased brains by X-ray fluorescence microscopy and diffraction"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3"],["dc.bibliographiccitation.volume","11113"],["dc.contributor.author","Robisch, Anna Lena"],["dc.contributor.author","Eckermann, Marina"],["dc.contributor.author","Töpperwien, Mareike"],["dc.contributor.author","Meer, Franziska van der"],["dc.contributor.author","Stadelmann-Nessler, Christine"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.editor","Müller, Bert"],["dc.contributor.editor","Wang, Ge"],["dc.date.accessioned","2020-04-23T12:45:49Z"],["dc.date.available","2020-04-23T12:45:49Z"],["dc.date.issued","2019"],["dc.description.abstract","X-ray cone-beam holo-tomography of unstained tissue from the human central nervous system reveals details down to sub-cellular length scales.1 This visualization of variations in the electron density of the sample is based on phase contrast techniques using intensities formed by self-interference of the beam between object and detector. Phase retrieval inverts diffraction and overcomes the phase problem by constraints such as several measurements at different Fresnel numbers for a single projection. Therefore, the object-to-detector distance (defocus) can be varied. However, for cone beam geometry, changing defocus changes magnification, which can be problematic in view of image processing and resolution. Alternatively, the photon energy can be altered (multi-E). Far from absorption edges, multi-E data yield the wavelength independent electron density. In this contribution we present multi-E holo-tomography at the GINIX setup of the P10 beamline at DESY. The instrument is based on a combined optics of elliptical mirrors and an x-ray waveguide positioned in the focal plane for further coherence, spatial Filtering and high numerical aperture.2 Previous results showed the suitability of this instrument for nanoscale tomography of unstained brain tissue.1 We demonstrate that upon energy variation, the focal spot is stable enough for imaging. To this end, a double crystal monochromator and automated alignment routines are required. Three tomograms of human brain tissue were recorded and jointly analyzed using phase retrieval based on the contrast transfer function formalism generalized to multiple photon energies. Variations of the electron density of the sample are successfully reconstructed. © (2019) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only."],["dc.identifier.doi","10.1117/12.2529041"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/64294"],["dc.language.iso","en"],["dc.notes.preprint","yes"],["dc.relation.eisbn","978-1-5106-2920-2"],["dc.relation.eventend","2019-09"],["dc.relation.eventlocation","San Diego"],["dc.relation.eventstart","2019-09"],["dc.relation.isbn","978-1-5106-2919-6"],["dc.relation.iserratumof","yes"],["dc.title","Nanoscale x-ray holo-tomography of human brain tissue with phase retrieval based on multiphoton energy recordings"],["dc.type","conference_paper"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e2113835118"],["dc.bibliographiccitation.issue","48"],["dc.bibliographiccitation.journal","Proceedings of the National Academy of Sciences"],["dc.bibliographiccitation.volume","118"],["dc.contributor.author","Eckermann, Marina"],["dc.contributor.author","Schmitzer, Bernhard"],["dc.contributor.author","van der Meer, Franziska"],["dc.contributor.author","Franz, Jonas"],["dc.contributor.author","Hansen, Ove"],["dc.contributor.author","Stadelmann, Christine"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2022-01-11T14:05:50Z"],["dc.date.available","2022-01-11T14:05:50Z"],["dc.date.issued","2021"],["dc.description.abstract","We have studied the three-dimensional (3D) cytoarchitecture of the human hippocampus in neuropathologically healthy and Alzheimer’s disease (AD) individuals, based on phase-contrast X-ray computed tomography of postmortem human tissue punch biopsies. In view of recent findings suggesting a nuclear origin of AD, we target in particular the nuclear structure of the dentate gyrus (DG) granule cells. Tissue samples of 20 individuals were scanned and evaluated using a highly automated approach of measurement and analysis, combining multiscale recordings, optimized phase retrieval, segmentation by machine learning, representation of structural properties in a feature space, and classification based on the theory of optimal transport. Accordingly, we find that the prototypical transformation between a structure representing healthy granule cells and the pathological state involves a decrease in the volume of granule cell nuclei, as well as an increase in the electron density and its spatial heterogeneity. The latter can be explained by a higher ratio of heterochromatin to euchromatin. Similarly, many other structural properties can be derived from the data, reflecting both the natural polydispersity of the hippocampal cytoarchitecture between different individuals in the physiological context and the structural effects associated with AD pathology."],["dc.identifier.doi","10.1073/pnas.2113835118"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/97758"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/369"],["dc.identifier.url","https://rdp.sfb274.de/literature/publications/53"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-507"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation","TRR 274: Checkpoints of Central Nervous System Recovery"],["dc.relation","TRR 274 | B01: The role of inflammatory cytokine signaling for efficient remyelination in multiple sclerosis"],["dc.relation.eissn","1091-6490"],["dc.relation.issn","0027-8424"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.relation.workinggroup","RG Stadelmann-Nessler"],["dc.rights","CC BY-NC-ND 4.0"],["dc.subject.gro","biomedical tomography"],["dc.title","Three-dimensional virtual histology of the human hippocampus based on phase-contrast computed tomography"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]