Salditt, Tim

[["dc.bibliographiccitation.firstpage","561"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Journal of Structural Biology"],["dc.bibliographiccitation.lastpage","568"],["dc.bibliographiccitation.volume","192"],["dc.contributor.author","Bartels, Matthias"],["dc.contributor.author","Krenkel, Martin"],["dc.contributor.author","Cloetens, Peter"],["dc.contributor.author","Möbius, Wiebke"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2017-09-07T11:54:52Z"],["dc.date.available","2017-09-07T11:54:52Z"],["dc.date.issued","2015"],["dc.description.abstract","We have used X-ray phase contrast tomography to resolve the structure of uncut, entire myelinated optic, saphenous and sciatic mouse nerves. Intrinsic electron density contrast suffices to identify axonal structures. Specific myelin labeling by an osmium tetroxide stain enables distinction between axon and surrounding myelin sheath. Utilization of spherical wave illumination enables zooming capabilities which enable imaging of entire sciatic intemodes as well as identification of sub-structures such as nodes of Ranvier and Schmidt-Lanterman incisures. (C) 2015 Elsevier Inc. All rights reserved."],["dc.identifier.doi","10.1016/j.jsb.2015.11.001"],["dc.identifier.gro","3141782"],["dc.identifier.isi","000365458400028"],["dc.identifier.pmid","26546551"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1013"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1095-8657"],["dc.relation.issn","1047-8477"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","biomedical tomography"],["dc.title","Myelinated mouse nerves studied by X-ray phase contrast zoom tomography"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.contributor.author","Töpperwien, Mareike"],["dc.contributor.author","Krenkel, Martin"],["dc.contributor.author","Quade, Felix"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.editor","Khounsary, Ali M."],["dc.contributor.editor","Dorssen, Gert E. van"],["dc.date.accessioned","2017-09-07T11:54:05Z"],["dc.date.available","2017-09-07T11:54:05Z"],["dc.date.issued","2016"],["dc.description.abstract","Due to the large penetration depth and small wavelength hard x-rays offer a unique potential for 3D biomedical and biological imaging, combining capabilities of high resolution and large sample volume. However, in classical absorption-based computed tomography, soft tissue only shows a weak contrast, limiting the actual resolution. With the advent of phase-contrast methods, the much stronger phase shift induced by the sample can now be exploited. For high resolution, free space propagation behind the sample is particularly well suited to make the phase shift visible. Contrast formation is based on the self-interference of the transmitted beam, resulting in object-induced intensity modulations in the detector plane. As this method requires a sufficiently high degree of spatial coherence, it was since long perceived as a synchrotron-based imaging technique. In this contribution we show that by combination of high brightness liquid-metal jet microfocus sources and suitable sample preparation techniques, as well as optimized geometry, detection and phase retrieval, excellent three-dimensional image quality can be obtained, revealing the anatomy of a cobweb spider in high detail. This opens up new opportunities for 3D virtual histology of small organisms. Importantly, the image quality is finally augmented to a level accessible to automatic 3D segmentation."],["dc.identifier.doi","10.1117/12.2246460"],["dc.identifier.gro","3145109"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2809"],["dc.language.iso","en"],["dc.notes.intern","Crossref Import"],["dc.notes.status","public"],["dc.publisher","SPIE"],["dc.publisher.place","Bellingham, Washington"],["dc.relation.conference","Advances in Laboratory-Based X-Ray Sources, Optics, and Applications"],["dc.relation.eventend","2016-08-31"],["dc.relation.eventlocation","San Diego, Calif."],["dc.relation.eventstart","2016-08-30"],["dc.relation.ispartof","Advances in Laboratory-Based X-Ray Sources, Optics, and Applications V"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","biomedical tomography"],["dc.title","Laboratory-based x-ray phase-contrast tomography enables 3D virtual histology"],["dc.type","conference_paper"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2220"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Optics Express"],["dc.bibliographiccitation.lastpage","2235"],["dc.bibliographiccitation.volume","21"],["dc.contributor.author","Krenkel, Martin"],["dc.contributor.author","Bartels, Matthias"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2017-09-07T11:48:18Z"],["dc.date.available","2017-09-07T11:48:18Z"],["dc.date.issued","2013"],["dc.description.abstract","We have implemented a deterministic method for solving the phase problem in hard x-ray in-line holography which overcomes the twin image problem. The phase distribution in the detector plane is retrieved by using two images with slightly different Fresnel numbers. We then use measured intensities and reconstructed phases in the detection plane to compute the exit wave in the sample plane. No further a priori information like a limited support or the assumption of pure phase objects is necessary so that it can be used for a wide range of complex samples. Using a nano-focused hard x-ray beam half period resolutions better than 30 nm are achieved. (C) 2013 Optical Society of America"],["dc.identifier.doi","10.1364/OE.21.002220"],["dc.identifier.gro","3142400"],["dc.identifier.isi","000315989500086"],["dc.identifier.pmid","23389203"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/7863"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1094-4087"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray imaging"],["dc.title","Transport of intensity phase reconstruction to solve the twin image problem in holographic x-ray imaging"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","033847"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Physical Review A"],["dc.bibliographiccitation.volume","89"],["dc.contributor.author","Ruhlandt, Aike"],["dc.contributor.author","Krenkel, Martin"],["dc.contributor.author","Bartels, Matthias"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2017-09-07T11:46:24Z"],["dc.date.available","2017-09-07T11:46:24Z"],["dc.date.issued","2014"],["dc.description.abstract","We present a solution to the phase problem in near-field x-ray (propagation) imaging. The three-dimensional complex-valued index of refraction is reconstructed from a set of projections recorded in the near-field (Fresnel) setting at a single detector distance. The solution is found by an iterative algorithm based only on the measured data and the three-dimensional tomographic (Helgason-Ludwig) consistency constraint without the need for further a priori knowledge or other restrictive assumptions."],["dc.identifier.doi","10.1103/PhysRevA.89.033847"],["dc.identifier.gro","3142164"],["dc.identifier.isi","000333410700008"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/5244"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1094-1622"],["dc.relation.issn","1050-2947"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","biomedical tomography"],["dc.title","Three-dimensional phase retrieval in propagation-based phase-contrast imaging"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","159901"],["dc.bibliographiccitation.issue","15"],["dc.bibliographiccitation.journal","Applied Physics Letters"],["dc.bibliographiccitation.volume","103"],["dc.contributor.author","Bartels, Matthias"],["dc.contributor.author","Hernandez, Victor H."],["dc.contributor.author","Krenkel, Martin"],["dc.contributor.author","Moser, Tobias"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2018-11-07T09:18:43Z"],["dc.date.available","2018-11-07T09:18:43Z"],["dc.date.issued","2013"],["dc.identifier.doi","10.1063/1.4821922"],["dc.identifier.isi","000325779700099"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/28467"],["dc.language.iso","en"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.issn","1077-3118"],["dc.relation.issn","0003-6951"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","biomedical tomography"],["dc.title","Phase contrast tomography of the mouse cochlea at microfocus x-ray sources"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","083703"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Applied Physics Letters"],["dc.bibliographiccitation.volume","103"],["dc.contributor.author","Bartels, Matthias"],["dc.contributor.author","Hernandez, Victor H."],["dc.contributor.author","Krenkel, Martin"],["dc.contributor.author","Moser, Tobias"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2017-09-07T11:47:37Z"],["dc.date.available","2017-09-07T11:47:37Z"],["dc.date.issued","2013"],["dc.description.abstract","We present phase contrast x-ray tomography of functional soft tissue within the bony cochlear capsule of mice, carried out at laboratory microfocus sources with well-matched source, detector, geometry, and reconstruction algorithms at spatial resolutions down to 2 mu m. Contrast, data quality and resolution enable the visualization of thin membranes and nerve fibers as well as automated segmentation of surrounding bone. By complementing synchrotron radiation imaging techniques, a broad range of biomedical applications becomes possible as demonstrated for optogenetic cochlear implant research. (C) 2013 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 Unported License."],["dc.identifier.doi","10.1063/1.4818737"],["dc.identifier.gro","3142304"],["dc.identifier.isi","000323788100094"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/6798"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0003-6951"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray optics"],["dc.subject.gro","x-ray imaging"],["dc.title","Phase contrast tomography of the mouse cochlea at microfocus x-ray sources"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","16"],["dc.bibliographiccitation.journal","Physics in Medicine and Biology"],["dc.bibliographiccitation.volume","57"],["dc.contributor.author","Olendrowitz, Christian"],["dc.contributor.author","Bartels, Matthias"],["dc.contributor.author","Krenkel, Martin"],["dc.contributor.author","Beerlink, André"],["dc.contributor.author","Mokso, R."],["dc.contributor.author","Sprung, Michael"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2017-09-07T11:48:27Z"],["dc.date.available","2017-09-07T11:48:27Z"],["dc.date.issued","2012"],["dc.description.abstract","We have analyzed the model organism Caenorhabditis elegans with the help of phase-contrast x-ray tomography. This work combines techniques from x-ray imaging studies of single biological cells by in-line holography with three-dimensional reconstruction and furthermore extends these studies to the multicellular level. To preserve the sub-cellular ultrastructure of the nematodes, we used the near-native sample preparation of high-pressure freezing as commonly used in the field of electron microscopy. For the presented samples, a standard, non-magnifying parallel-beam setting, as well as a magnifying, divergent-beam setting using nanofocusing optics is evaluated based on their tomographic reconstruction potential. In this paper, we address difficulties in sample preparation and issues of image processing. By experimental refinement and through optimized reconstruction procedures, we were able to perform x-ray imaging studies on a living specimen."],["dc.identifier.doi","10.1088/0031-9155/57/16/5309"],["dc.identifier.gro","3142478"],["dc.identifier.isi","000307112600017"],["dc.identifier.pmid","22853964"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/8729"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0031-9155"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","biomedical tomography"],["dc.title","Phase-contrast x-ray imaging and tomography of the nematode Caenorhabditis elegans"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","96"],["dc.bibliographiccitation.lastpage","97"],["dc.bibliographiccitation.volume","Life Sciences and Cultural Heritage, Elettra - SYRMEP"],["dc.contributor.author","Dullin, Christian"],["dc.contributor.author","dal Monego, Simeone"],["dc.contributor.author","Larsson, Emanuel"],["dc.contributor.author","Mohammadi, Sara"],["dc.contributor.author","Krenkel, Martin"],["dc.contributor.author","Garrovo, Chiara"],["dc.contributor.author","Biffi, Stefania"],["dc.contributor.author","Lorenzon, Andrea"],["dc.contributor.author","Markus, Andrea"],["dc.contributor.author","Napp, Joanna"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","Accardo, Agostino"],["dc.contributor.author","Alves, Frauke"],["dc.contributor.author","Tromba, G."],["dc.date.accessioned","2020-03-11T09:34:33Z"],["dc.date.available","2020-03-11T09:34:33Z"],["dc.date.issued","2015"],["dc.description.abstract","We successfully applied synchrotron in-line phase contrast CT in combination with single distance phase retrieval to image lungs of asthmatic and healthy mice in high resolution in situ. The increased image quality in phase contrast CT added functional contrast to CT which enabled simultaneous i\\) anatomical imaging for the discrimination of asthmatic from healthy mice based on alterations of their lung structure as well as ii\\) functional imaging to track the location of barium sulfate loaded intratracheally instilled alveolar macrophages. Link to fulltext: http://www.elettra.eu/images/Documents/SCIENCE/Elettra%20HL%202015.pdf"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/63295"],["dc.language.iso","en"],["dc.publisher","Elettra - Sincrotrone Trieste"],["dc.publisher.place","Trieste"],["dc.relation.ispartof","ELETTRA HIGHLIGHTS 2014-2015"],["dc.title","Simultaneous localization of labelled macrophages and structural analysis of asthmatic mice by phase contrast CT"],["dc.type","book_chapter"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","282"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Acta Crystallographica Section A Foundations and Advances"],["dc.bibliographiccitation.lastpage","292"],["dc.bibliographiccitation.volume","73"],["dc.contributor.author","Krenkel, Martin"],["dc.contributor.author","Toepperwien, Mareike"],["dc.contributor.author","Alves, Frauke"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2022-03-01T11:47:06Z"],["dc.date.available","2022-03-01T11:47:06Z"],["dc.date.issued","2017"],["dc.description.abstract","X-ray tomography at the level of single biological cells is possible in a low-dose regime, based on full-field holographic recordings, with phase contrast originating from free-space wave propagation. Building upon recent progress in cellular imaging based on the illumination by quasi-point sources provided by X-ray waveguides, here this approach is extended in several ways. First, the phase-retrieval algorithms are extended by an optimized deterministic inversion, based on a multi-distance recording. Second, different advanced forms of iterative phase retrieval are used, operational for single-distance and multi-distance recordings. Results are compared for several different preparations of macrophage cells, for different staining and labelling. As a result, it is shown that phase retrieval is no longer a bottleneck for holographic imaging of cells, and how advanced schemes can be implemented to cope also with high noise and inconsistencies in the data."],["dc.description.abstract","X-ray tomography at the level of single biological cells is possible in a low-dose regime, based on full-field holographic recordings, with phase contrast originating from free-space wave propagation. Building upon recent progress in cellular imaging based on the illumination by quasi-point sources provided by X-ray waveguides, here this approach is extended in several ways. First, the phase-retrieval algorithms are extended by an optimized deterministic inversion, based on a multi-distance recording. Second, different advanced forms of iterative phase retrieval are used, operational for single-distance and multi-distance recordings. Results are compared for several different preparations of macrophage cells, for different staining and labelling. As a result, it is shown that phase retrieval is no longer a bottleneck for holographic imaging of cells, and how advanced schemes can be implemented to cope also with high noise and inconsistencies in the data."],["dc.identifier.doi","10.1107/S2053273317007902"],["dc.identifier.doi","10.1107/s2053273317007902"],["dc.identifier.gro","3142469"],["dc.identifier.pii","S2053273317007902"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103911"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-531"],["dc.notes.status","final"],["dc.relation.eissn","2053-2733"],["dc.relation.issn","2053-2733"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights.uri","http://creativecommons.org/licenses/by/2.0/uk/legalcode"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","biomedical tomography"],["dc.title","Three-dimensional single-cell imaging with X-ray waveguides in the holographic regime"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","no"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","612"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Journal of Applied Crystallography"],["dc.bibliographiccitation.lastpage","620"],["dc.bibliographiccitation.volume","50"],["dc.contributor.author","Nicolas, Jan-David"],["dc.contributor.author","Bernhardt, Marten"],["dc.contributor.author","Krenkel, Martin"],["dc.contributor.author","Richter, Claudia"],["dc.contributor.author","Luther, Stefan"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2018-04-23T11:48:59Z"],["dc.date.available","2018-04-23T11:48:59Z"],["dc.date.issued","2017"],["dc.description.abstract","This article presents scanning small-angle X-ray scattering (SAXS) experiments on the actomyosin assemblies in freeze-dried neo-natal rat cardiac muscle cells. By scanning the cells through a sub-micrometre focused beam, the local structure and filament orientation can be probed and quantified. To this end, SAXS data were recorded and analyzed directly in reciprocal space to generate maps of different structural parameters (scanning SAXS). The scanning SAXS experiments were complemented by full-field holographic imaging of the projected electron density, following a slight rearrangement of the instrumental setup. It is shown that X-ray holography is ideally suited to complete missing scattering data at low momentum transfer in the structure factor, extending the covered range of spatial frequencies by two orders of magnitude. Regions of interest for scanning can be easily selected on the basis of the electron density maps. Finally, the combination of scanning SAXS and holography allows for a direct verification of possible radiation-induced structural changes in the cell."],["dc.identifier.doi","10.1107/s1600576717003351"],["dc.identifier.gro","3142473"],["dc.identifier.pii","S1600576717003351"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/13624"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103908"],["dc.language.iso","en"],["dc.notes.intern","lifescience updates Crossref Import"],["dc.notes.status","final"],["dc.relation.eissn","1600-5767"],["dc.relation.issn","1600-5767"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights.uri","http://journals.iucr.org/services/copyrightpolicy.html"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","x-ray scattering"],["dc.title","Combined scanning X-ray diffraction and holographic imaging of cardiomyocytes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","no"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]