Zahur, Muzna

[["dc.bibliographiccitation.firstpage","15"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Advances in Bioscience and Biotechnology"],["dc.bibliographiccitation.lastpage","20"],["dc.bibliographiccitation.volume","04"],["dc.contributor.author","Zahur, Muzna"],["dc.contributor.author","Asif, Muhammad Ahsan"],["dc.contributor.author","Zeeshan, Nadia"],["dc.contributor.author","Mehmood, Sajid"],["dc.contributor.author","Malik, Muhammad Faheem"],["dc.contributor.author","Asif, Abdul R."],["dc.date.accessioned","2013-10-07T15:54:20Z"],["dc.date.accessioned","2021-10-27T13:20:04Z"],["dc.date.available","2013-10-07T15:54:20Z"],["dc.date.available","2021-10-27T13:20:04Z"],["dc.date.issued","2013-10"],["dc.description.abstract","Transcription factors play key roles in plant develop- ment and stress responses through their interaction with cis-elements and/or other transcription factors. Homeodomain associated leucine zipper proteins (HD-Zip) constitute a family of transcription factors that are characterized by the presence of a DNA- binding domain closely linked with leucine zipper motif functioning in dimer formation. This type of association is unique to plants and considered as an excellent candidate to activate developmental respons- es to altering environmental conditions. Cotton is the most important fiber plant with a lot of local and commercial uses in the world. HD-Zip proteins not only have key roles in different stages of vascular and inter-fascicular fiber differentiation of cotton but also are suggested to have an important role against abio- tic stress that is one of the key factors limiting cotton productivity. Plants have developed various strategies to manage stress conditions through a combination of metabolic, physiological and morphological adapta- tions. These adaptive changes rely largely on altera- tions in gene expression. Therefore, transcriptional regulators play a crucial role in stress tolerance. Be- ing a transcription factor HD-Zip might be a useful target for genetic engineering to generate multiple stress tolerance in susceptible plants. In the following chapter, we discussed how the HD-Zip proteins would play a useful role for cotton development both in fiber production and stress adaptation."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2013"],["dc.format.extent","6"],["dc.identifier.doi","10.4236/abb.2013.410A3003"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9360"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/91935"],["dc.language.iso","en"],["dc.notes.intern","Migrated from goescholar"],["dc.relation.issn","2156-8502"],["dc.relation.issn","2156-8456"],["dc.relation.orgunit","Universitätsmedizin Göttingen"],["dc.rights","CC BY-NC 3.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc/3.0"],["dc.title","Homeobox leucine zipper proteins and cotton improvement"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","866"],["dc.bibliographiccitation.issue","09"],["dc.bibliographiccitation.journal","Advances in Bioscience and Biotechnology"],["dc.bibliographiccitation.lastpage","871"],["dc.bibliographiccitation.volume","04"],["dc.contributor.author","Mushtaq, Asim"],["dc.contributor.author","Tariq, Mujahid Azeem"],["dc.contributor.author","Rasheed, Umer"],["dc.contributor.author","Afroz, Amber"],["dc.contributor.author","Zeeshan, Nadia"],["dc.contributor.author","Asif, Abdul R."],["dc.contributor.author","Zahur, Muzna"],["dc.date.accessioned","2019-07-09T11:54:34Z"],["dc.date.available","2019-07-09T11:54:34Z"],["dc.date.issued","2013"],["dc.description.abstract","Hepatitis C has a 3% of the global disease burden that remains endemic in many regions of the world. According to a general statistical survey it has approximately 5.3% seroprevalence in Pakistan. HCV is a persistent and silent disease thus making the primary diagnosis complicated. Occasionally HCV positive population could not be diagnosed by routine HCV antibody testing therefore requires molecular diagnosis. This study is aimed to determine the prevalence of HCV and to estimate the HCV viral load by quantitative analysis among different patient groups of District Gujrat, Pakistan. A total of 597 samples were collected from clinically diagnosed liver patients that were categorized into three age groups: 1\\) up to 25 years; 2\\) 26-50 years 3\\) above 50 years. All samples were subjected to real time PCR for determination and quantification of HCV RNA. Activity of liver aminotransferases was measured. The overall prevalence of HCV-RNA was 73.87%. Females had slightly higher HCV prevalence which is 74.06% while 73.45% in males. Highest prevalence of active HCV infection was found in age group 26-50. In addition, liver function tests showed that 28.12% HCV-positive patients do not have elevated ALT level whereas 32.65% did not show elevated AST levels. It may be assumed that there is not a significant relationship between increased viral load and liver amino transferases. The study concluded a significantly higher rate of HCV infection in young population. Moreover screening with antibody and liver function tests alone does not exclude the possibility of HCV infection."],["dc.format.extent","6"],["dc.identifier.doi","10.4236/abb.2013.49115"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9300"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/60680"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","2156-8502"],["dc.rights","CC BY-NC 3.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc/3.0"],["dc.title","Estimation of HCV viral load and liver enzymes among different patients groups of District Gujrat, Pakistan"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Pakistan Journal of Botany"],["dc.bibliographiccitation.volume","51"],["dc.contributor.author","Jamal, Adil"],["dc.contributor.author","Shahid, Muhammad Naveed"],["dc.contributor.author","Aftab, Beenish"],["dc.contributor.author","Zahur, Muzna"],["dc.contributor.author","Rashid, Bushra"],["dc.contributor.author","Johargy, Ayman Khalid"],["dc.contributor.author","Husnain, Tayyab"],["dc.date.accessioned","2020-12-10T18:43:55Z"],["dc.date.available","2020-12-10T18:43:55Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.30848/PJB2019-4(14)"],["dc.identifier.eissn","2070-3368"],["dc.identifier.issn","0556-3321"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/78268"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Generation and analysis of expressed sequence tags from roots cDNA library of cotton (Gossypium arboreum)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e56246"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Biswas, Sagarika"],["dc.contributor.author","Sharma, Saurabh"],["dc.contributor.author","Saroha, Ashish"],["dc.contributor.author","Bhakuni, D. S."],["dc.contributor.author","Malhotra, Rajesh"],["dc.contributor.author","Zahur, Muzna"],["dc.contributor.author","Oellerich, Michael"],["dc.contributor.author","Das, Hasi R."],["dc.contributor.author","Asif, Abdul R."],["dc.date.accessioned","2018-11-07T09:28:08Z"],["dc.date.available","2018-11-07T09:28:08Z"],["dc.date.issued","2013"],["dc.description.abstract","Rheumatoid arthritis (RA) is a chronic, autoimmune and inflammatory joint disease with a poorly understood etiology. Despite widespread diagnostic use of anti-citrullinated protein antibodies and rheumatoid factor proteins there is a strong demand for novel serological biomarkers to improve the diagnosis this disease. The present study was aimed to identify novel autoantigens involved in rheumatoid arthritis (RA) pathogenesis through immune-proteomic strategy. Synovial fluid samples from clinically diagnosed RA patients were separated on two-dimensional gel electrophoresis (2-DE). Samples from patients with non-RA rheumatisms (osteoarthritis and trauma) were used as controls. Immunoreactive proteins were spotted by Western blotting followed by identification through Q-TOF mass spectrometer analysis. Forty Western blots were generated using plasma from ten individual RA patients and 33 reactive spots were identified, 20 from the high molecular weight (HMW) gel and 13 from the low molecular weight (LMW) gel. Among the 33 common immunogenic spots, 18 distinct autoantigens were identified, out of which 14 are novel proteins in this context. Expression analysis of five important proteins, vimentin, gelsolin, alpha 2 HS glycoprotein (AHSG), glial fibrillary acidic protein (GFAP), and alpha 1 beta-glycoprotein (A1BG) by Western blot analysis using their specific antibodies revealed their higher expression in RA synovial fluid as compared to non-RA samples. Recombinantly expressed GFAP and A1BG protein were used to develop an in-house ELISA to quantify the amount of autoantibodies in the RA patients. RA patients revealed an increase in the expression of GFAP and A1BG in the plasma as compared to osteoarthritis patients. Therefore, GFAP and A1BG can be proposed as potential new autoantigens of diagnostic importance for RA subjects. Further characterization of these proteins in rheumatoid arthritis will be helpful in understanding the role of these proteins in the disease pathogenesis providing new diagnostic tool with better specificity and accurate detection of the disease."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2013"],["dc.identifier.doi","10.1371/journal.pone.0056246"],["dc.identifier.isi","000315970300137"],["dc.identifier.pmid","23418544"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/8662"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/30706"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 3.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/3.0"],["dc.title","Identification of Novel Autoantigen in the Synovial Fluid of Rheumatoid Arthritis Patients Using an Immunoproteomics Approach"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","29"],["dc.bibliographiccitation.journal","Electronic Journal of Biotechnology"],["dc.bibliographiccitation.lastpage","36"],["dc.bibliographiccitation.volume","34"],["dc.contributor.author","Zeeshan, Nadia"],["dc.contributor.author","Naz, Saher"],["dc.contributor.author","Naz, Shumaila"],["dc.contributor.author","Afroz, Amber"],["dc.contributor.author","Zahur, Muzna"],["dc.contributor.author","Zia, Safia"],["dc.date.accessioned","2019-07-09T11:45:49Z"],["dc.date.available","2019-07-09T11:45:49Z"],["dc.date.issued","2018"],["dc.description.abstract","insubstantial amount for industrial applications. Endo-1, 4-β-glucanases (Egl) is one of the major enzyme involved in degradation of cellulose, an important component of plant cell wall. The present study was aimed at enhancing the production of endo-1, 4-β-glucanases (Egl) of Bacillus halodurans in Escherichia coli. Results: A putative Egl gene of Bacillus Halodurans was expressed in E. coli by cloning in pET 22b (+).On induction with isopropyl-b-D-1-thiogalactopyranoside, the enzyme expression reached upto ~20% of the cell protein producing 29.2 mg/liter culture. An increase in cell density to 12 in auto-inducing LB medium (absorbance at 600 nm) enhanced β-glucanase production up to 5.4 fold. The molecular mass of the enzyme was determined to be 39 KDa, which is nearly the same as the calculated value. Protein sequence was analyzed by CDD, Pfam, I TASSER, COACH, PROCHECK Servers and putative amino acids involved in the formation of catalytic, substrate and metal binding domains were identified. Phylogenetic analysis of the β-glucanases of B. halodurans was performed and position of Egl among other members of the genus Bacillus producing endo-glucanases was determined. Temperature and pH optima of the enzyme were found to be 60°C and 8.0, respectively, under the assay conditions. Conclusion: Production of endo-1, 4 β-glucanase enzymes fromB. halodurans increased several foldswhen cloned in pET vector and expressed in E. coli. To our knowledge, this is the first report of high-level expression and characterization of an endo-1, 4 β-glucanases from B. halodurans"],["dc.identifier.doi","10.1016/j.ejbt.2018.05.001"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15322"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59315"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.rights","CC BY-NC-ND 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc-nd/4.0"],["dc.subject.ddc","610"],["dc.title","Heterologous expression and enhanced production of β-1,4-glucanase of Bacillus halodurans C-125 in Escherichia coli"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","142"],["dc.bibliographiccitation.journal","Frontiers in Molecular Neuroscience"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Zahur, Muzna"],["dc.contributor.author","Tolö, Johan"],["dc.contributor.author","Bähr, Mathias"],["dc.contributor.author","Kügler, Sebastian"],["dc.date.accessioned","2018-04-23T11:46:58Z"],["dc.date.available","2018-04-23T11:46:58Z"],["dc.date.issued","2017"],["dc.description.abstract","Gene editing tools like TALENs, ZFNs and Crispr/Cas now offer unprecedented opportunities for targeted genetic manipulations in virtually all species. Most of the recent research in this area has concentrated on manipulation of the genome in isolated cells, which then give rise to transgenic animals or modified stem cell lines. Much less is known about applicability of genetic scissors in terminally differentiated, non-dividing cells like neurons of the adult brain. We addressed this question by expression of a pair of ZFNs targeting the murine cathepsin D gene in CNS neurons by means of an optimized AAV viral vector. We show that ZFN expression resulted in substantial depletion of cathepsin D from neuronal lysosomes, demonstrating a robust gene deletion. Importantly, long-term ZFN expression in CNS neurons did not impair essential neuronal functionality and did not cause inflammation or neurodegeneration, suggesting that potent genetic scissors can be expressed safely in the mouse brain. This finding opens up new venues to create novel research models for neurodegenerative disorders."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2017"],["dc.identifier.doi","10.3389/fnmol.2017.00142"],["dc.identifier.gro","3142072"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14497"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/13278"],["dc.language.iso","en"],["dc.notes.intern","lifescience updates Crossref Import"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.relation.issn","1662-5099"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Long-Term Assessment of AAV-Mediated Zinc Finger Nuclease Expression in the Mouse Brain"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]