Janshoff, Andreas

[["dc.bibliographiccitation.artnumber","eaat1161"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Science Advances"],["dc.bibliographiccitation.volume","4"],["dc.contributor.author","Block, Johanna"],["dc.contributor.author","Witt, Hannes"],["dc.contributor.author","Candelli, Andrea"],["dc.contributor.author","Danes, Jordi Cabanas"],["dc.contributor.author","Peterman, Erwin J. G."],["dc.contributor.author","Wuite, Gijs J. L."],["dc.contributor.author","Janshoff, Andreas"],["dc.contributor.author","Köster, Sarah"],["dc.date.accessioned","2019-07-09T11:45:54Z"],["dc.date.available","2019-07-09T11:45:54Z"],["dc.date.issued","2018"],["dc.description.abstract","Structure and dynamics of living matter rely on design principles fundamentally different from concepts of traditional material science. Specialized intracellular filaments in the cytoskeleton permit living systems to divide, migrate, and growwith a high degree of variability and durability. Among the three filament systems,microfilaments,microtubules, and intermediate filaments (IFs), the physical properties of IFs and their role in cellular mechanics are the least well understood. We use optical trapping of individual vimentin filaments to investigate energy dissipation, strain history dependence, and creep behavior of stretched filaments. By stochastic and numerical modeling, we link our experimental observations to the peculiar molecular architecture of IFs. We find that individual vimentin filaments display tensile memory and are able to dissipate more than 70% of the input energy.We attribute these phenomena to distinct nonequilibrium folding and unfolding of a helices in the vimentin monomers constituting the filaments."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2018"],["dc.identifier.doi","10.1126/sciadv.aat1161"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15341"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59334"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","2375-2548"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.orgunit","Fakultät für Physik"],["dc.relation.workinggroup","RG Köster (Cellular Biophysics)"],["dc.rights","CC BY-NC 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0/"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","530"],["dc.subject.gro","cytoskeleton"],["dc.subject.gro","cellular biophysics"],["dc.title","Viscoelastic properties of vimentin originate from nonequilibrium conformational changes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","048101"],["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Physical Review Letters"],["dc.bibliographiccitation.lastpage","5"],["dc.bibliographiccitation.volume","118"],["dc.contributor.author","Block, Johanna"],["dc.contributor.author","Witt, Hannes"],["dc.contributor.author","Candelli, Andrea"],["dc.contributor.author","Peterman, Erwin J. G."],["dc.contributor.author","Wuite, Gijs J. L."],["dc.contributor.author","Janshoff, Andreas"],["dc.contributor.author","Köster, Sarah"],["dc.date.accessioned","2020-12-10T18:25:42Z"],["dc.date.available","2020-12-10T18:25:42Z"],["dc.date.issued","2017"],["dc.description.abstract","The mechanical properties of eukaryotic cells are to a great extent determined by the cytoskeleton, a composite network of different filamentous proteins. Among these, intermediate filaments (IFs) are exceptional in their molecular architecture and mechanical properties. Here we directly record stress-strain curves of individual vimentin IFs using optical traps and atomic force microscopy. We find a strong loading rate dependence of the mechanical response, supporting the hypothesis that IFs could serve to protect eukaryotic cells from fast, large deformations. Our experimental results show different unfolding regimes, which we can quantitatively reproduce by an elastically coupled system of multiple two-state elements."],["dc.identifier.doi","10.1103/PhysRevLett.118.048101"],["dc.identifier.eissn","1079-7114"],["dc.identifier.fs","623737"],["dc.identifier.issn","0031-9007"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17056"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/75797"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.relation.eissn","1079-7114"],["dc.relation.issn","0031-9007"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Köster (Cellular Biophysics)"],["dc.rights","CC BY 3.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/3.0/"],["dc.subject.gro","cytoskeleton"],["dc.subject.gro","cellular biophysics"],["dc.title","Nonlinear Loading-Rate-Dependent Force Response of Individual Vimentin Intermediate Filaments to Applied Strain"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","unknown"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","e2102026118"],["dc.bibliographiccitation.issue","27"],["dc.bibliographiccitation.journal","Proceedings of the National Academy of Sciences of the United States of America"],["dc.bibliographiccitation.volume","118"],["dc.contributor.author","Schepers, Anna V."],["dc.contributor.author","Lorenz, Charlotta"],["dc.contributor.author","Nietmann, Peter"],["dc.contributor.author","Janshoff, Andreas"],["dc.contributor.author","Klumpp, Stefan"],["dc.contributor.author","Köster, Sarah"],["dc.date.accessioned","2021-07-01T12:23:07Z"],["dc.date.available","2021-07-01T12:23:07Z"],["dc.date.issued","2021-07-06"],["dc.description.abstract","The cytoskeleton, an intricate network of protein filaments, motor proteins, and cross-linkers, largely determines the mechanical properties of cells. Among the three filamentous components, F-actin, microtubules, and intermediate filaments (IFs), the IF network is by far the most extensible and resilient to stress. We present a multiscale approach to disentangle the three main contributions to vimentin IF network mechanics—single-filament mechanics, filament length, and interactions between filaments—including their temporal evolution. Combining particle tracking, quadruple optical trapping, and computational modeling, we derive quantitative information on the strength and kinetics of filament interactions. Specifically, we find that hydrophobic contributions to network mechanics enter mostly via filament-elongation kinetics, whereas electrostatics have a direct influence on filament–filament interactions."],["dc.identifier.doi","10.1073/pnas.2102026118"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/87447"],["dc.language.iso","en"],["dc.relation.issn","0027-8424"],["dc.relation.issn","1091-6490"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Köster (Cellular Biophysics)"],["dc.subject.gro","cytoskeleton"],["dc.subject.gro","cellular biophysics"],["dc.title","Multiscale mechanics and temporal evolution of vimentin intermediate filament networks"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","7349"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Nano Letters"],["dc.bibliographiccitation.lastpage","7356"],["dc.bibliographiccitation.volume","19"],["dc.contributor.author","Forsting, Johanna"],["dc.contributor.author","Kraxner, Julia"],["dc.contributor.author","Witt, Hannes"],["dc.contributor.author","Janshoff, Andreas"],["dc.contributor.author","Köster, Sarah"],["dc.date.accessioned","2020-03-04T13:30:18Z"],["dc.date.available","2020-03-04T13:30:18Z"],["dc.date.issued","2019"],["dc.description.abstract","Intermediate filaments (IFs) are part of the cytoskeleton of eukaryotic cells and, therefore, are largely responsible for the cell's mechanical properties. IFs are characterized by a pronounced extensibility and remarkable resilience that enable them to support cells in extreme situations. Previous experiments showed that, under strain, α-helices in vimentin IFs might unfold to β-sheets. Upon repeated stretching, the filaments soften; however, the remaining plastic strain is negligible. Here, we observe that vimentin IFs do not recover their original stiffness on reasonable time scales, and we explain these seemingly contradicting results by introducing a third, less well-defined conformational state. Reversibility on the nanoscale can be fully rescued by introducing cross-linkers that prevent transition to the β-sheet. Our results classify IFs as a nanomaterial with intriguing mechanical properties, which is likely to play a major role for the cell's local adaption to external stimuli."],["dc.identifier.doi","10.1021/acs.nanolett.9b02972"],["dc.identifier.pmid","31498648"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/63104"],["dc.language.iso","en"],["dc.relation.eissn","1530-6992"],["dc.relation.issn","1530-6984"],["dc.relation.issn","1530-6992"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Köster (Cellular Biophysics)"],["dc.subject.gro","cytoskeleton"],["dc.subject.gro","cellular biophysics"],["dc.title","Vimentin Intermediate Filaments Undergo Irreversible Conformational Changes during Cyclic Loading"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]