Lugert, Raimond

[["dc.bibliographiccitation.firstpage","728"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Bundesgesundheitsblatt - Gesundheitsforschung - Gesundheitsschutz"],["dc.bibliographiccitation.lastpage","734"],["dc.bibliographiccitation.volume","54"],["dc.contributor.author","Alter, T."],["dc.contributor.author","Bereswill, S."],["dc.contributor.author","Gluender, G."],["dc.contributor.author","Haag, L.-M."],["dc.contributor.author","Haenel, I."],["dc.contributor.author","Heimesaat, Markus M."],["dc.contributor.author","Lugert, Raimond"],["dc.contributor.author","Rautenschlein, Silke"],["dc.contributor.author","Weber, R. M."],["dc.contributor.author","Zautner, Andreas Erich"],["dc.contributor.author","Gross, U."],["dc.date.accessioned","2018-11-07T08:55:46Z"],["dc.date.available","2018-11-07T08:55:46Z"],["dc.date.issued","2011"],["dc.description.abstract","Over the last few years, infections with Campylobacter have significantly increased in Europe and Germany and these bacteria have even surpassed Salmonella as the most prevalent bacteria, causing gastroenteritis. Especially contamination during the handling and consumption of meat products seems to be the most important risk factor which plays a prominent role for transmission to man. In addition, contact with pets and other animals, drinking raw or improperly pasteurized milk, and the tenacity of Campylobacter in different environments, especially water, have also to be considered for an adequate risk assessment. Besides gastroenteritis, arthralgia, and Guillain-Barre syndrome are important clinical complications of Campylobacter infections in man. At the same time, it is mostly unclear why the course of infection in man and in reservoir animals differs significantly, especially as only a few classical bacterial virulence factors have been identified so far. For these reasons, the development of efficient prevention strategies is of utmost importance in order to control campylobacteriosis."],["dc.identifier.doi","10.1007/s00103-011-1289-y"],["dc.identifier.isi","000292038500010"],["dc.identifier.pmid","21626378"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/22983"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","1436-9990"],["dc.title","Campylobacteriosis of man. Livestock as reservoir for Campylobacter species"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","International Journal of Medical Microbiology"],["dc.bibliographiccitation.volume","294"],["dc.contributor.author","Mueller, N."],["dc.contributor.author","Lugert, Raimond"],["dc.contributor.author","Kuhns, Martin"],["dc.contributor.author","Polch, T."],["dc.contributor.author","Gross, U."],["dc.date.accessioned","2018-11-07T10:45:57Z"],["dc.date.available","2018-11-07T10:45:57Z"],["dc.date.issued","2004"],["dc.format.extent","88"],["dc.identifier.isi","000224456000015"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/47628"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Urban & Fischer Verlag"],["dc.publisher.place","Jena"],["dc.relation.conference","Annual Meeting of the DGHM"],["dc.relation.eventlocation","Munster, GERMANY"],["dc.relation.issn","1438-4221"],["dc.title","Expression and localization of type III-secreted proteins of Chlamydia pneumoniae"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","163"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Medical Microbiology and Immunology"],["dc.bibliographiccitation.lastpage","171"],["dc.bibliographiccitation.volume","193"],["dc.contributor.author","Lugert, Raimond"],["dc.contributor.author","Kuhns, Martin"],["dc.contributor.author","Polch, T."],["dc.contributor.author","Gross, U."],["dc.date.accessioned","2018-11-07T10:44:24Z"],["dc.date.available","2018-11-07T10:44:24Z"],["dc.date.issued","2004"],["dc.description.abstract","The entire developmental cycle of the obligate intracellular bacteria Chlamydia pneumoniae takes place within the inclusion body. As many gram negative bacteria, Chlamydia possess a type III-secretion system (TTSS), which allows them to target effector molecules into the host cell. The expression and localization of several proteins constituting the TTSS apparatus and of proteins supposed to be secreted by the TTSS have been investigated. For the TTSS-constituting proteins, we selected representatives such as YscN (ATPase), LcrE (putative \"lid\" of the TTSS) and LcrH1 (postulated to be a chaperone). Furthermore, we focused on the putative effector proteins IncA, IncB, IncC, Cpn0809 and Cpn1020. Expression of these proteins was detected by reverse transcriptase-PCR followed by immunoblot analysis using antisera that were generated against the corresponding recombinant proteins. Thereby, expression could be detected on the RNA and/or protein level. Intracellular localization of proteins under investigation was determined by immunofluorescence assays using the respective antisera. YscN was shown to be distributed equally throughout the inclusion body, whereas LcrE gave a more prominent staining of the inclusion membrane. IncA was detected mainly on the membrane of the inclusion body, whereas IncB and IncC were shown to be located within the inclusion. Immunofluorescence assays with antisera raised against Cpn0809 and Cpn1020 showed completely different labeling. Signals corresponding to Cpn0809 and Cpn1020 were distributed within the host cell rather than inside the inclusions. Taken together, the different localization patterns of the effector proteins indicate differences in function and interplay with the host cell."],["dc.identifier.doi","10.1007/s00430-003-0206-x"],["dc.identifier.isi","000225206800002"],["dc.identifier.pmid","14593477"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/47258"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","0300-8584"],["dc.title","Expression and localization of type III secretion-related proteins of Chlamydia pneumoniae"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","172"],["dc.bibliographiccitation.journal","BMC Pregnancy and Childbirth"],["dc.bibliographiccitation.volume","17"],["dc.contributor.author","Voelker, Fabian M."],["dc.contributor.author","Cooper, Paul"],["dc.contributor.author","Bader, Oliver"],["dc.contributor.author","Uy, Angela"],["dc.contributor.author","Zimmermann, Ortrud"],["dc.contributor.author","Lugert, Raimond"],["dc.contributor.author","Gross, Uwe"],["dc.date.accessioned","2018-11-07T10:22:50Z"],["dc.date.available","2018-11-07T10:22:50Z"],["dc.date.issued","2017"],["dc.description.abstract","Background: Although infectious diseases still account for a high burden of morbidity and mortality in sub-Saharan Africa, simultaneous investigations on multiple infections affecting maternal and child health are missing. Methods: We conducted a cross-sectional, single-centre pilot study in a rural area of Ghana to assess the infectiological profile during pregnancy. Screening of 180 expectant mothers was done by vaginal swabs and serology to detect the most common pregnancy-relevant infections. They were also interviewed for potential risk factors, outcome of previous pregnancies, and socio-economic aspects. Results: We found a high prevalence of infections caused by hepatitis B virus (16.7% HBs antigen positive). In contrast, infections caused by hepatitis C virus (1.1% anti-HCV) and HIV (0.6%) were rare. Maternal malaria was frequent (10.6%), despite increasing acceptance of intermittent preventive treatment during pregnancy (IPTp). Group B streptococci were present in 10.6% of all pregnant women. Absence of antibodies against varicella zoster virus in 43.2%, Toxoplasma gondii in 26.8%, parvovirus B19 in 20.0%, and rubella virus in 15.7% makes a significant proportion of pregnant women susceptible for acquiring primary infections. Whereas all study participants had specific IgG antibodies against human cytomegalovirus, infections with Listeria, Brucella, or Neisseria gonorrhoeae as well as active syphilis were absent. Conclusions: Our pilot study in a rural community in Ghana indicates an urgent need for action in dealing at least with high-prevalent pregnancy-relevant infections, such as hepatitis B, malaria and those caused by group B streptococci. In addition, the resulting prevalence rates of various other infections may offer guidance for health officials to prioritize possible future intervention schemes."],["dc.identifier.doi","10.1186/s12884-017-1351-3"],["dc.identifier.isi","000402791800001"],["dc.identifier.pmid","28583150"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14507"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/42347"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1471-2393"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Prevalence of pregnancy-relevant infections in a rural setting of Ghana"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Mycoses"],["dc.bibliographiccitation.volume","54"],["dc.contributor.author","Bader, Oliver"],["dc.contributor.author","Lugert, Raimond"],["dc.contributor.author","Kuhns, Martin"],["dc.contributor.author","Reichard, Utz"],["dc.contributor.author","Weig, Michael S."],["dc.contributor.author","Gross, U."],["dc.date.accessioned","2018-11-07T08:52:29Z"],["dc.date.available","2018-11-07T08:52:29Z"],["dc.date.issued","2011"],["dc.format.extent","403"],["dc.identifier.isi","000294878900056"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/22171"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.publisher.place","Malden"],["dc.relation.issn","0933-7407"],["dc.title","Azole antifungal drug resistance in clinical Aspergillus fumigatus isolates as a consequence of azole use in agriculture in Germany?"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","387"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Medical Microbiology and Immunology"],["dc.bibliographiccitation.lastpage","396"],["dc.bibliographiccitation.volume","197"],["dc.contributor.author","Mueller, Nicole"],["dc.contributor.author","Sattelmacher, Florian"],["dc.contributor.author","Lugert, Raimond"],["dc.contributor.author","Gross, Uwe"],["dc.date.accessioned","2018-11-07T11:08:44Z"],["dc.date.available","2018-11-07T11:08:44Z"],["dc.date.issued","2008"],["dc.description.abstract","We here describe four proteins of Chlamydia pneumoniae, which might play a role in host-pathogen interaction. The hypothetical bacterial proteins CPn0708 and CPn0712 were detected in Chlamydia pneumoniae-infected host cells by indirect immunofluorescence tests with polyclonal antisera raised against the respective proteins. While CPn0708 was localized within the inclusion body, CPn0712 was identified in the inclusion membrane and in the surrounding host cell cytosol. CPn0712 colocalizes with actin, indicating its possible interaction with components of the cytoskeleton. Investigations on CPn0809 and CPn1020, two Chlamydia pneumoniae proteins previously described to be secreted into the host cell cytosol, revealed colocalization with calnexin, a marker for the ER. Neither CPn0712, CPn0809 nor CPn1020 were able to inhibit host cell apoptosis. Furthermore, transient expression of CPn0712, CPn0809 and CPn1020 by the host cell itself had no effect on subsequent infection with Chlamydia pneumoniae. However, microarray analysis of CPn0712-expressing host cells revealed six host cell genes which were regulated as in host cells infected with Chlamydia pneumoniae, indicating the principal usefulness of heterologous expression to study the effect of Chlamydia pneumoniae proteins on host cell modulation."],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft [906/8-2]"],["dc.identifier.doi","10.1007/s00430-008-0097-y"],["dc.identifier.isi","000258760500007"],["dc.identifier.pmid","18449565"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?goescholar/3097"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/52858"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","0300-8584"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Characterization and intracellular localization of putative Chlamydia pneumoniae effector proteins"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","24"],["dc.bibliographiccitation.journal","International Journal of Medical Microbiology"],["dc.bibliographiccitation.lastpage","25"],["dc.bibliographiccitation.volume","302"],["dc.contributor.author","Zautner, Andreas Erich"],["dc.contributor.author","Johann, C."],["dc.contributor.author","Strubel, A."],["dc.contributor.author","Busse, C."],["dc.contributor.author","Tareen, Abdul Malik"],["dc.contributor.author","Lugert, Raimond"],["dc.contributor.author","Schmidt-Ott, Ruprecht"],["dc.contributor.author","Gross, U."],["dc.date.accessioned","2018-11-07T09:06:02Z"],["dc.date.available","2018-11-07T09:06:02Z"],["dc.date.issued","2012"],["dc.identifier.isi","000311593300082"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/25464"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Gmbh, Urban & Fischer Verlag"],["dc.publisher.place","Jena"],["dc.relation.conference","64th Annual Meeting of the German-Society-for-Hygiene-and-Microbiology (DGHM)"],["dc.relation.eventlocation","Hamburg, GERMANY"],["dc.relation.issn","1438-4221"],["dc.title","Serodiagnostic and Seroprevalence of Campylobacteriosis and post-Campylobacter-sequelae"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1359"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Clinical Microbiology and Infection"],["dc.bibliographiccitation.lastpage","1365"],["dc.bibliographiccitation.volume","17"],["dc.contributor.author","Bader, O."],["dc.contributor.author","Weig, M."],["dc.contributor.author","Taverne-Ghadwal, L."],["dc.contributor.author","Lugert, R."],["dc.contributor.author","Groß, U."],["dc.contributor.author","Kuhns, M."],["dc.date.accessioned","2021-06-01T10:47:18Z"],["dc.date.available","2021-06-01T10:47:18Z"],["dc.date.issued","2011"],["dc.identifier.doi","10.1111/j.1469-0691.2010.03398.x"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/85555"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.issn","1198-743X"],["dc.title","Improved clinical laboratory identification of human pathogenic yeasts by matrix-assisted laser desorption ionization time-of-flight mass spectrometry"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","1088"],["dc.bibliographiccitation.journal","BMC Genomics"],["dc.bibliographiccitation.volume","16"],["dc.contributor.author","Zautner, Andreas Erich"],["dc.contributor.author","Goldschmidt, Anne-Marie"],["dc.contributor.author","Thürmer, Andrea"],["dc.contributor.author","Schuldes, Jörg"],["dc.contributor.author","Bader, Oliver"],["dc.contributor.author","Lugert, Raimond"],["dc.contributor.author","Groß, Uwe"],["dc.contributor.author","Stingl, Kerstin"],["dc.contributor.author","Salinas, Gabriela"],["dc.contributor.author","Lingner, Thomas"],["dc.date.accessioned","2018-11-07T09:47:24Z"],["dc.date.available","2018-11-07T09:47:24Z"],["dc.date.issued","2015"],["dc.description.abstract","Background: Campylobacter species are the most prevalent bacterial pathogen causing acute enteritis worldwide. In contrast to Campylobacter jejuni, about 5 % of Campylobacter coli strains exhibit susceptibility to restriction endonuclease digestion by DpnI cutting specifically 5'-G(m)ATC-3' motifs. This indicates significant differences in DNA methylation between both microbial species. The goal of the study was to analyze the methylome of a C. coli strain susceptible to DpnI digestion, to identify its methylation motifs and restriction modification systems (RM-systems), and compare them to related organisms like C. jejuni and Helicobacter pylori. Results: Using one SMRT cell and the PacBio RS sequencing technology followed by PacBio Modification and Motif Analysis the complete genome of the DpnI susceptible strain C. coli BfR-CA-9557 was sequenced to 500-fold coverage and assembled into a single contig of 1.7 Mbp. The genome contains a CJIE1-like element prophage and is phylogenetically closer to C. coli clade 1 isolates than clade 3. 45,881 6-methylated adenines (ca. 2.7 % of genome positions) that are predominantly arranged in eight different methylation motifs and 1,788 4-methylated cytosines (ca. 0.1 %) have been detected. Only two of these motifs correspond to known restriction modification motifs. Characteristic for this methylome was the very high fraction of methylation of motifs with mostly above 99 %. Conclusions: Only five dominant methylation motifs have been identified in C. jejuni, which have been associated with known RM-systems. C. coli BFR-CA-9557 shares one (RAATTY) of these, but four ORFs could be assigned to putative Type I RM-systems, seven ORFs to Type II RM-systems and three ORFs to Type IV RM-systems. In accordance with DpnI prescreening RM-system IIP, methylation of GATC motifs was detected in C. coli BfR-CA-9557. A homologous IIP RM-system has been described for H. pylori. The remaining methylation motifs are specific for C. coli BfR-CA-9557 and have been neither detected in C. jejuni nor in H. pylori. The results of this study give us new insights into epigenetics of Campylobacteraceae and provide the groundwork to resolve the function of RM-systems in C. coli."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2015"],["dc.identifier.doi","10.1186/s12864-015-2317-3"],["dc.identifier.isi","000367061700011"],["dc.identifier.pmid","26689587"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13469"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/35106"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.issn","1471-2164"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","SMRT sequencing of the Campylobacter coli BfR-CA-9557 genome sequence reveals unique methylation motifs"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1019"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","European Journal of Clinical Microbiology & Infectious Diseases"],["dc.bibliographiccitation.lastpage","1027"],["dc.bibliographiccitation.volume","33"],["dc.contributor.author","Zautner, Andreas Erich"],["dc.contributor.author","Johann, C."],["dc.contributor.author","Strubel, A."],["dc.contributor.author","Busse, C."],["dc.contributor.author","Tareen, Abdul Malik"],["dc.contributor.author","Masanta, Wycliffe Omurwa"],["dc.contributor.author","Lugert, Raimond"],["dc.contributor.author","Schmidt-Ott, Ruprecht"],["dc.contributor.author","Gross, U."],["dc.date.accessioned","2018-11-07T09:39:47Z"],["dc.date.available","2018-11-07T09:39:47Z"],["dc.date.issued","2014"],["dc.description.abstract","Post-infectious sequelea such as Guillain Barr, syndrome (GBS), reactive arthritis (RA), and inflammatory bowel disease (IBD) may arise as a consequence of acute Campylobacter-enteritis (AE). However, reliable seroprevalence data of Campylobacter-associated sequelae has not been established. The objectives of this study were, first, to identify the most specific and sensitive test antigen in an optimized ELISA assay for diagnosing a previous Campylobacter-infection and, second, to compare the prevalence of anti-Campylobacter antibodies in cohorts of healthy blood donors (BD), AE, GBS, RA, and IBD patients with antibodies against known GBS, RA and IBD triggering pathogens. Optimized ELISAs of single and combined Campylobacter-proteins OMP18 and P39 as antigens were prepared and sera from AE, GBS, RA and IBD patients and BD were tested for Campylobcter-specific IgA and IgG antibodies. The results were compared with MIKROGEN (TM)-recomLine Campylobacter IgA/IgG and whole cell lysate-immunoblot. Antibodies specific for Helicobacter pylori, Mycoplasma pneumoniae, Yersinia enterocolitica, and Borrelia afzelii were tested with commercial immunoblots. ROC plot analysis revealed AUC maxima in the combination of OMP18 and P39 for IgA and in the P39-antigen for IgG. As a result, 34-49 % GBS cases, 44-62 % RA cases and 23-40 % IBD cases were associated with Campylobacter-infection. These data show that Campylobcater-seropositivity in these patient groups is significantly higher than other triggering pathogens suggesting that it plays an important role in development of GBS and RA, and supports the hypothesis that recurrent acute campylobacteriosis triggers IBD."],["dc.identifier.doi","10.1007/s10096-013-2040-4"],["dc.identifier.isi","000335743500017"],["dc.identifier.pmid","24413899"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9697"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/33365"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","1435-4373"],["dc.relation.issn","0934-9723"],["dc.rights","CC BY 2.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.0"],["dc.title","Seroprevalence of campylobacteriosis and relevant post-infectious sequelae"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]