Ta, Haisen

[["dc.bibliographiccitation.firstpage","571"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Nature Methods"],["dc.bibliographiccitation.lastpage","573"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Vicidomini, Giuseppe"],["dc.contributor.author","Moneron, Gael"],["dc.contributor.author","Han, Kyu Young"],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Ta, Haisen"],["dc.contributor.author","Reuss, Matthias"],["dc.contributor.author","Engelhardt, Johann"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:44:10Z"],["dc.date.available","2017-09-07T11:44:10Z"],["dc.date.issued","2011"],["dc.description.abstract","Applying pulsed excitation together with time-gated detection improves the fluorescence on-off contrast in continuous-wave stimulated emission depletion (CW-STED) microscopy, thus revealing finer details in fixed and living cells using moderate light intensities. This method also enables super-resolution fluorescence correlation spectroscopy with CW-STED beams, as demonstrated by quantifying the dynamics of labeled lipid molecules in the plasma membrane of living cells."],["dc.identifier.doi","10.1038/NMETH.1624"],["dc.identifier.gro","3142707"],["dc.identifier.isi","000292194500020"],["dc.identifier.pmid","21642963"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/141"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1548-7091"],["dc.title","Sharper low-power STED nanoscopy by time gating"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","ACS chemical biology"],["dc.bibliographiccitation.lastpage","6"],["dc.bibliographiccitation.volume","13"],["dc.contributor.author","Butkevich, Alexey N."],["dc.contributor.author","Ta, Haisen"],["dc.contributor.author","Ratz, Michael"],["dc.contributor.author","Stoldt, Stefan"],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2018-01-17T13:21:24Z"],["dc.date.available","2018-01-17T13:21:24Z"],["dc.date.issued","2017"],["dc.description.abstract","A 810 nm STED nanoscopy setup and an appropriate combination of two fluorescent dyes (Si-rhodamine 680SiR and carbopyronine 610CP) have been developed for near-IR live-cell super-resolution imaging. Vimentin endogenously tagged using the CRISPR/Cas9 approach with the SNAP tag, together with a noncovalent tubulin label, provided reliable and cell-to-cell reproducible dual-color confocal and STED imaging of the cytoskeleton in living cells."],["dc.identifier.doi","10.1021/acschembio.7b00616"],["dc.identifier.pmid","28933823"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/11709"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation.eissn","1554-8937"],["dc.title","Two-Color 810 nm STED Nanoscopy of Living Cells with Endogenous SNAP-Tagged Fusion Proteins"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2469"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Chemistry - A European Journal"],["dc.bibliographiccitation.lastpage","2475"],["dc.bibliographiccitation.volume","23"],["dc.contributor.author","Meineke, Dirk N. H."],["dc.contributor.author","Bossi, Mariano L."],["dc.contributor.author","Ta, Haisen"],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2018-01-17T13:36:35Z"],["dc.date.available","2018-01-17T13:36:35Z"],["dc.date.issued","2017-02-16"],["dc.description.abstract","Electronic energy transfer (EET) between chromophores is of fundamental importance for many biological processes and optoelectronic devices. However, common models fall short in fully describing the process, especially in bichromophoric model systems with a donor and acceptor connected by a rigid linker providing perpendicular geometries. Herein, we report a novel strategy for preparing bichromophores containing adamantane or 2-(2-adamantylidene)adamantane as rigid spacers, providing a fixed distance between chromophores, and their parallel or perpendicular arrangement without chromophore rotation. New fluorophores were developed and linked via spiroatoms. Bichromophores with identical (blue-blue) or different (blue-red) chromophores were synthesized, either in orthogonal or parallel geometry. These were characterized by absorption/fluorescence spectroscopy, time-resolved fluorescence anisotropy, and fluorescence antibunching measurements. Based on the Förster point-dipole approximation, EET efficiencies were estimated by using geometrical parameters from (time-dependent) density functional calculations. For bichromophores with parallel geometry, the predicted EET efficiencies were near unity and fit the measurements. In spite of estimated values around 0.4 and 0.5, 100 % efficiency was observed also for bichromophores with orthogonal geometry. The new rigid scaffolds presented here open new possibilities for the synthesis of bichormophores with well-defined parallel or perpendicular geometry."],["dc.identifier.doi","10.1002/chem.201605587"],["dc.identifier.pmid","27922726"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/11723"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation.eissn","1521-3765"],["dc.title","Bichromophoric Compounds with Orthogonally and Parallelly Arranged Chromophores Separated by Rigid Spacers"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","6117"],["dc.bibliographiccitation.issue","24"],["dc.bibliographiccitation.journal","Proceedings of the National Academy of Sciences"],["dc.bibliographiccitation.lastpage","6122"],["dc.bibliographiccitation.volume","115"],["dc.contributor.author","Eilers, Yvan"],["dc.contributor.author","Ta, Haisen"],["dc.contributor.author","Gwosch, Klaus C."],["dc.contributor.author","Balzarotti, Francisco"],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2022-03-01T11:46:24Z"],["dc.date.available","2022-03-01T11:46:24Z"],["dc.date.issued","2018"],["dc.description.abstract","Compared with localization schemes solely based on evaluating patterns of molecular emission, the recently introduced single-molecule localization concept called MINFLUX and the fluorescence nanoscopies derived from it require up to orders of magnitude fewer emissions to attain single-digit nanometer resolution. Here, we demonstrate that the lower number of required fluorescence photons enables MINFLUX to detect molecular movements of a few nanometers at a temporal sampling of well below 1 millisecond. Using fluorophores attached to thermally fluctuating DNA strands as model systems, we demonstrate that measurement times as short as 400 microseconds suffice to localize fluorescent molecules with ∼2-nm precision. Such performance is out of reach for popular camera-based localization by centroid calculation of emission diffraction patterns. Since theoretical limits have not been reached, our results show that emerging MINFLUX nanoscopy bears great potential for dissecting the motions of individual (macro)molecules at hitherto-unattained combinations of spatial and temporal resolution."],["dc.description.abstract","Compared with localization schemes solely based on evaluating patterns of molecular emission, the recently introduced single-molecule localization concept called MINFLUX and the fluorescence nanoscopies derived from it require up to orders of magnitude fewer emissions to attain single-digit nanometer resolution. Here, we demonstrate that the lower number of required fluorescence photons enables MINFLUX to detect molecular movements of a few nanometers at a temporal sampling of well below 1 millisecond. Using fluorophores attached to thermally fluctuating DNA strands as model systems, we demonstrate that measurement times as short as 400 microseconds suffice to localize fluorescent molecules with ∼2-nm precision. Such performance is out of reach for popular camera-based localization by centroid calculation of emission diffraction patterns. Since theoretical limits have not been reached, our results show that emerging MINFLUX nanoscopy bears great potential for dissecting the motions of individual (macro)molecules at hitherto-unattained combinations of spatial and temporal resolution."],["dc.identifier.doi","10.1073/pnas.1801672115"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103658"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-531"],["dc.relation.eissn","1091-6490"],["dc.relation.issn","0027-8424"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc-nd/4.0/"],["dc.title","MINFLUX monitors rapid molecular jumps with superior spatiotemporal resolution"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","5912"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Nano Letters"],["dc.bibliographiccitation.lastpage","5918"],["dc.bibliographiccitation.volume","15"],["dc.contributor.author","Vicidomini, Giuseppe"],["dc.contributor.author","Ta, Haisen"],["dc.contributor.author","Honigmann, Alf"],["dc.contributor.author","Mueller, Veronika"],["dc.contributor.author","Clausen, Mathias P."],["dc.contributor.author","Waithe, Dominic"],["dc.contributor.author","Galiani, Silvia"],["dc.contributor.author","Sezgin, Erdinc"],["dc.contributor.author","Diaspro, Alberto"],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Eggeling, Christian"],["dc.date.accessioned","2017-09-07T11:43:34Z"],["dc.date.available","2017-09-07T11:43:34Z"],["dc.date.issued","2015"],["dc.description.abstract","Heterogeneous diffusion dynamics of molecules play an important role in many cellular signaling events, such as of lipids in plasma membrane bioactivity. However, these dynamics can often only be visualized by single-molecule and super-resolution optical microscopy techniques. Using fluorescence lifetime correlation spectroscopy (FLCS, an extension of fluorescence correlation spectroscopy, FCS) on a super-resolution stimulated emission depletion (STED) microscope, we here extend previous observations of nanoscale lipid dynamics in the plasma membrane of living mammalian cells. STED-FLCS allows an improved determination of spatiotemporal heterogeneity in molecular diffusion and interaction dynamics via a novel gated detection scheme, as demonstrated by a comparison between STED-FLCS and previous conventional STED-FCS recordings on fluorescent phosphoglycerolipid and sphingolipid analogues in the plasma membrane of live mammalian cells. The STED-FLCS data indicate that biophysical and biochemical parameters such as the affinity for molecular complexes strongly change over space and time within a few seconds. Drug treatment for cholesterol depletion or actin cytoskeleton depolymerization not only results in the already previously observed decreased affinity for molecular interactions but also in a slight reduction of the spatiotemporal heterogeneity. STED-FLCS specifically demonstrates a significant improvement over previous gated STED-FCS experiments and with its improved spatial and temporal resolution is a novel tool for investigating how heterogeneities of the cellular plasma membrane may regulate biofunctionality."],["dc.identifier.doi","10.1021/acs.nanolett.5b02001"],["dc.identifier.gro","3141837"],["dc.identifier.isi","000361252700038"],["dc.identifier.pmid","26235350"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1623"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Amer Chemical Soc"],["dc.relation.eissn","1530-6992"],["dc.relation.issn","1530-6984"],["dc.title","STED-FLCS: An Advanced Tool to Reveal Spatiotemporal Heterogeneity of Molecular Membrane Dynamics"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","9365"],["dc.bibliographiccitation.issue","30"],["dc.bibliographiccitation.journal","Journal of the American Chemical Society"],["dc.bibliographiccitation.lastpage","9368"],["dc.bibliographiccitation.volume","138"],["dc.contributor.author","Lukinavicius, Grazvydas"],["dc.contributor.author","Reymond, Luc"],["dc.contributor.author","Umezawa, Keitaro"],["dc.contributor.author","Sallin, Olivier"],["dc.contributor.author","D'Este, E."],["dc.contributor.author","Goettfert, Fabian"],["dc.contributor.author","Ta, Haisen"],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Urano, Yasuteru"],["dc.contributor.author","Johnsson, Kai"],["dc.date.accessioned","2017-09-07T11:44:44Z"],["dc.date.available","2017-09-07T11:44:44Z"],["dc.date.issued","2016"],["dc.description.abstract","Here we present a far-red, silicon-rhodamine-based fluorophore (SiR700) for live-cell multicolor imaging. SiR700 has excitation and emission maxima at 690 and 715 nm, respectively. SiR700-based probes for F-actin, microtubules, lysosomes, and SNAP-tag are fluorogenic, cell-pertneable, and compatible with super resolution microscopy. In conjunction with probes based on the previously introduced carboxy-SiR650, SiR700-based probes permit multicolor live-cell superresolution microscopy in the far-red, thus significantly expanding our capacity for imaging living cells."],["dc.identifier.doi","10.1021/jacs.6b04782"],["dc.identifier.gro","3141639"],["dc.identifier.isi","000381062600008"],["dc.identifier.pmid","27420907"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/4122"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Amer Chemical Soc"],["dc.relation.issn","0002-7863"],["dc.title","Fluorogenic Probes for Multicolor Imaging in Living Cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","13344"],["dc.bibliographiccitation.issue","38"],["dc.bibliographiccitation.journal","Chemistry - A European Journal"],["dc.bibliographiccitation.lastpage","13356"],["dc.bibliographiccitation.volume","21"],["dc.contributor.author","Kolmakov, Kirill"],["dc.contributor.author","Hebisch, Elke"],["dc.contributor.author","Wolfram, Thomas"],["dc.contributor.author","Nordwig, Lars A."],["dc.contributor.author","Wurm, Christian Andreas"],["dc.contributor.author","Ta, Haisen"],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:43:32Z"],["dc.date.available","2017-09-07T11:43:32Z"],["dc.date.issued","2015"],["dc.description.abstract","Far-red emitting fluorescent dyes for optical microscopy, stimulated emission depletion (STED), and ground-state depletion (GSDIM) super-resolution microscopy are presented. Fluorinated silicon-rhodamines (SiRF dyes) and phosphorylated oxazines have absorption and emission maxima at about approximate to 660 and 680nm, respectively, possess high photostability, and large fluorescence quantum yields in water. A high-yielding synthetic path to introduce three aromatic fluorine atoms and unconventional conjugation/solubilization spacers into the scaffold of a silicon-rhodamine is described. The bathochromic shift in SiRF dyes is achieved without additional fused rings or double bonds. As a result, the molecular size and molecular mass stay quite small (<600Da). The use of the =800nm STED beam instead of the commonly used one at =750-775nm provides excellent imaging performance and suppresses re-excitation of SiRF and the oxazine dyes. The photophysical properties and immunofluorescence imaging performance of these new far-red emitting dyes (photobleaching, optical resolution, and switch-off behavior) are discussed in detail and compared with those of some well-established fluorophores with similar spectral properties."],["dc.identifier.doi","10.1002/chem.201501394"],["dc.identifier.gro","3141827"],["dc.identifier.isi","000360829600028"],["dc.identifier.pmid","26272226"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1512"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1521-3765"],["dc.relation.issn","0947-6539"],["dc.title","Far-Red Emitting Fluorescent Dyes for Optical Nanoscopy: Fluorinated Silicon-Rhodamines (SiRF Dyes) and Phosphorylated Oxazines"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","5412"],["dc.bibliographiccitation.journal","Nature Communications"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Honigmann, Alf"],["dc.contributor.author","Mueller, Veronika"],["dc.contributor.author","Ta, Haisen"],["dc.contributor.author","Schoenle, Andreas"],["dc.contributor.author","Sezgin, Erdinc"],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Eggeling, Christian"],["dc.date.accessioned","2017-09-07T11:45:25Z"],["dc.date.available","2017-09-07T11:45:25Z"],["dc.date.issued","2014"],["dc.description.abstract","The interaction of lipids and proteins plays an important role in plasma membrane bioactivity, and much can be learned from their diffusion characteristics. Here we present the combination of super-resolution STED microscopy with scanning fluorescence correlation spectroscopy (scanning STED-FCS, sSTED-FCS) to characterize the spatial and temporal heterogeneity of lipid interactions. sSTED-FCS reveals transient molecular interaction hotspots for a fluorescent sphingolipid analogue. The interaction sites are smaller than 80nm in diameter and lipids are transiently trapped for several milliseconds in these areas. In comparison, newly developed fluorescent phospholipid and cholesterol analogues with improved phase-partitioning properties show more homogenous diffusion, independent of the preference for liquid-ordered or disordered membrane environments. Our results do not support the presence of nanodomains based on lipid-phase separation in the basal membrane of our cultured nonstimulated cells, and show that alternative interactions are responsible for the strong local trapping of our sphingolipid analogue."],["dc.identifier.doi","10.1038/ncomms6412"],["dc.identifier.gro","3142023"],["dc.identifier.isi","000345624800033"],["dc.identifier.pmid","25410140"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/3690"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","2041-1723"],["dc.title","Scanning STED-FCS reveals spatiotemporal heterogeneity of lipid interaction in the plasma membrane of living cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e54421"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Vicidomini, Giuseppe"],["dc.contributor.author","Schoenle, Andreas"],["dc.contributor.author","Ta, Haisen"],["dc.contributor.author","Han, Kyu Young"],["dc.contributor.author","Moneron, Gael"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:48:18Z"],["dc.date.available","2017-09-07T11:48:18Z"],["dc.date.issued","2013"],["dc.description.abstract","In a stimulated emission depletion (STED) microscope the region in which fluorescence markers can emit spontaneously shrinks with continued STED beam action after a singular excitation event. This fact has been recently used to substantially improve the effective spatial resolution in STED nanoscopy using time-gated detection, pulsed excitation and continuous wave (CW) STED beams. We present a theoretical framework and experimental data that characterize the time evolution of the effective point-spread-function of a STED microscope and illustrate the physical basis, the benefits, and the limitations of time-gated detection both for CW and pulsed STED lasers. While gating hardly improves the effective resolution in the all-pulsed modality, in the CW-STED modality gating strongly suppresses low spatial frequencies in the image. Gated CW-STED nanoscopy is in essence limited (only) by the reduction of the signal that is associated with gating. Time-gated detection also reduces/suppresses the influence of local variations of the fluorescence lifetime on STED microscopy resolution."],["dc.identifier.doi","10.1371/journal.pone.0054421"],["dc.identifier.gro","3142402"],["dc.identifier.isi","000313872800053"],["dc.identifier.pmid","23349884"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/7885"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1932-6203"],["dc.title","STED Nanoscopy with Time-Gated Detection: Theoretical and Experimental Aspects"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","731"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Nature Methods"],["dc.bibliographiccitation.lastpage","733"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Lukinavicius, Grazvydas"],["dc.contributor.author","Reymond, Luc"],["dc.contributor.author","D'Este, Elisa"],["dc.contributor.author","Masharina, Anastasiya"],["dc.contributor.author","Göttfert, Fabian"],["dc.contributor.author","Ta, Haisen"],["dc.contributor.author","Güther, Angelika"],["dc.contributor.author","Fournier, Mathias"],["dc.contributor.author","Rizzo, Stefano"],["dc.contributor.author","Waldmann, Herbert"],["dc.contributor.author","Blaukopf, Claudia"],["dc.contributor.author","Sommer, Christoph"],["dc.contributor.author","Gerlich, Daniel Wolfram"],["dc.contributor.author","Arndt, Hans-Dieter"],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Johnsson, Kai"],["dc.date.accessioned","2017-09-07T11:46:11Z"],["dc.date.available","2017-09-07T11:46:11Z"],["dc.date.issued","2014"],["dc.description.abstract","We introduce far-red, fluorogenic probes that combine minimal cytotoxicity with excellent brightness and photostability for fluorescence imaging of actin and tubulin in living cells. Applied in stimulated emission depletion (STED) microscopy, they reveal the ninefold symmetry of the centrosome and the spatial organization of actin in the axon of cultured rat neurons with a resolution unprecedented for imaging cytoskeletal structures in living cells."],["dc.identifier.doi","10.1038/NMETH.2972"],["dc.identifier.gro","3142098"],["dc.identifier.isi","000338321400015"],["dc.identifier.pmid","24859753"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/4522"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1548-7105"],["dc.relation.issn","1548-7091"],["dc.title","Fluorogenic probes for live-cell imaging of the cytoskeleton"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]