Proteomes in Primordia Development of Coprinopsis cinerea

Fruiting body formation of Coprinopsis cinerea takes place at 25 ℃ under a 12 h day/12 h night regime. It starts by intense local hyphal branching with production of primary hyphal knots in the dark. A first light signal induces the transfer into the compact secondary hyphal knots. In these secondary hyphal knots, cap and stipe tissue begin to differentiate. This process underlies distinct patterns of light and dark regulated events over the following 5 days and then the mushrooms mature on the next day. To gain insight into the complex cytological processes during the cap and stipe tissue development, we isolated total proteomes from distinct primordia stages for MS/MS analysis. Between 1672 and 2663 proteins were detected in the different samples with at least two peptides at a confidence level of 99%, among which 1401 proteins were shared by all the samples. Known proteins in primordia development were identified in the samples.