Nanoscopy in a Living Mouse Brain

ISSN
0036-8075
Date Issued
2012
Author(s)
DOI
10.1126/science.1215369
Abstract
We demonstrated superresolution optical microscopy in a living higher animal. Stimulated emission depletion (STED) fluorescence nanoscopy reveals neurons in the cerebral cortex of a mouse with <70-nanometer resolution. Dendritic spines and their subtle changes can be observed at their relevant scales over extended periods of time.