Berning, Sebastian

[["dc.bibliographiccitation.firstpage","551"],["dc.bibliographiccitation.issue","6068"],["dc.bibliographiccitation.journal","Science"],["dc.bibliographiccitation.lastpage","551"],["dc.bibliographiccitation.volume","335"],["dc.contributor.author","Berning, Sebastian"],["dc.contributor.author","Willig, Katrin I."],["dc.contributor.author","Steffens, Heinz"],["dc.contributor.author","Dibaj, Payam"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:49:00Z"],["dc.date.available","2017-09-07T11:49:00Z"],["dc.date.issued","2012"],["dc.description.abstract","We demonstrated superresolution optical microscopy in a living higher animal. Stimulated emission depletion (STED) fluorescence nanoscopy reveals neurons in the cerebral cortex of a mouse with <70-nanometer resolution. Dendritic spines and their subtle changes can be observed at their relevant scales over extended periods of time."],["dc.identifier.doi","10.1126/science.1215369"],["dc.identifier.gro","3142583"],["dc.identifier.isi","000299769200034"],["dc.identifier.pmid","22301313"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/8950"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0036-8075"],["dc.title","Nanoscopy in a Living Mouse Brain"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","L01"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","L03"],["dc.bibliographiccitation.volume","105"],["dc.contributor.author","Göttfert, Fabian"],["dc.contributor.author","Wurm, Christian Andreas"],["dc.contributor.author","Mueller, Veronika"],["dc.contributor.author","Berning, Sebastian"],["dc.contributor.author","Cordes, Volker C."],["dc.contributor.author","Honigmann, Alf"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:47:39Z"],["dc.date.available","2017-09-07T11:47:39Z"],["dc.date.issued","2013"],["dc.description.abstract","We report on a fiber laser-based stimulated emission-depletion microscope providing down to similar to 20 nm resolution in raw data images as well as 15-19 nm diameter probing areas in fluorescence correlation spectroscopy. Stimulated emission depletion pulses of nanosecond duration and 775 nm wavelength are used to silence two fluorophores simultaneously, ensuring offset-free colocalization analysis. The versatility of this superresolution method is exemplified by revealing the octameric arrangement of Xenopus nuclear pore complexes and by quantifying the diffusion of labeled lipid molecules in artificial and living cell membranes."],["dc.identifier.doi","10.1016/j.bpj.2013.05.029"],["dc.identifier.gro","3142327"],["dc.identifier.isi","000321241400001"],["dc.identifier.pmid","23823248"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/7053"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: Korber Foundation"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0006-3495"],["dc.title","Coaligned Dual-Channel STED Nanoscopy and Molecular Diffusion Analysis at 20 nm Resolution"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","7"],["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Optical Nanoscopy"],["dc.bibliographiccitation.lastpage","7"],["dc.bibliographiccitation.volume","1"],["dc.contributor.author","Wurm, Christian Andreas"],["dc.contributor.author","Kolmakov, Kirill"],["dc.contributor.author","Göttfert, Fabian"],["dc.contributor.author","Ta, Haisen"],["dc.contributor.author","Bossi, Mariano"],["dc.contributor.author","Schill, Heiko"],["dc.contributor.author","Berning, Sebastian"],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Donnert, Gerald"],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2017-09-07T11:53:03Z"],["dc.date.available","2017-09-07T11:53:03Z"],["dc.date.issued","2012"],["dc.description.abstract","In optical microscopy, most red-emitting dyes provide only moderate performance due to unspecific binding, poor labeling efficiency, and insufficient brightness. Here we report on four novel red fluororescent dyes, including the first phosphorylated dye, created by combining a rigidized rhodamine backbone with various polar groups. They exhibit large fluorescence quantum yields and improved NHS ester stability. While these fluorophores are highly suitable for fluorescence microscopy in general, they excel in stimulated emission depletion (STED) microscopy, providing < 25 nm spatial resolution in raw images of cells."],["dc.identifier.doi","10.1186/2192-2853-1-7"],["dc.identifier.fs","593636"],["dc.identifier.gro","3145019"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/8898"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2709"],["dc.language.iso","en"],["dc.notes.intern","Crossref Import"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.relation.issn","2192-2853"],["dc.relation.orgunit","Fakultät für Physik"],["dc.rights","Goescholar"],["dc.rights.uri","https://goedoc.uni-goettingen.de/licenses"],["dc.title","Novel red fluorophores with superior performance in STED microscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","no"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1130"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Cell Reports"],["dc.bibliographiccitation.lastpage","1145"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Agarwal, Amit"],["dc.contributor.author","Zhang, Mingyue"],["dc.contributor.author","Trembak-Duff, Irina"],["dc.contributor.author","Unterbarnscheidt, Tilmann"],["dc.contributor.author","Radyushkin, Konstantin"],["dc.contributor.author","Dibaj, Payam"],["dc.contributor.author","Souza, Daniel Martins de"],["dc.contributor.author","Boretius, Susann"],["dc.contributor.author","Brzózka, Magdalena"],["dc.contributor.author","Steffens, Heinz"],["dc.contributor.author","Berning, Sebastian"],["dc.contributor.author","Teng, Zenghui"],["dc.contributor.author","Gummert, Maike N."],["dc.contributor.author","Tantra, Martesa"],["dc.contributor.author","Guest, Peter C."],["dc.contributor.author","Willig, Katrin I."],["dc.contributor.author","Frahm, Jens"],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Bahn, Sabine"],["dc.contributor.author","Rossner, Moritz J."],["dc.contributor.author","Nave, Klaus-Armin"],["dc.contributor.author","Ehrenreich, Hannelore"],["dc.contributor.author","Zhang, Weiqi"],["dc.contributor.author","Schwab, Markus H."],["dc.date.accessioned","2017-09-07T11:45:36Z"],["dc.date.available","2017-09-07T11:45:36Z"],["dc.date.issued","2014"],["dc.description.abstract","Neuregulin-1 (NRG1) gene variants are associated with increased genetic risk for schizophrenia. It is unclear whether risk haplotypes cause elevated or decreased expression of NRG1 in the brains of schizophrenia patients, given that both findings have been reported from autopsy studies. To study NRG1 functions in vivo, we generated mouse mutants with reduced and elevated NRG1 levels and analyzed the impact on cortical functions. Loss of NRG1 from cortical projection neurons resulted in increased inhibitory neurotransmission, reduced synaptic plasticity, and hypoactivity. Neuronal overexpression of cysteine-rich domain (CRD)-NRG1, the major brain isoform, caused unbalanced excitatory-inhibitory neurotransmission, reduced synaptic plasticity, abnormal spine growth, altered steady-state levels of synaptic plasticity-related proteins, and impaired sensorimotor gating. We conclude that an \"optimal' level of NRG1 signaling balances excitatory and inhibitory neurotransmission in the cortex. Our data provide a potential pathomechanism for impaired synaptic plasticity and suggest that human NRG1 risk haplotypes exert a gain-of-function effect."],["dc.identifier.doi","10.1016/j.celrep.2014.07.026"],["dc.identifier.gro","3142068"],["dc.identifier.isi","000341573500021"],["dc.identifier.pmid","25131210"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/4189"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Cell Press"],["dc.relation.issn","2211-1247"],["dc.title","Dysregulated Expression of Neuregulin-1 by Cortical Pyramidal Neurons Disrupts Synaptic Plasticity"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]