A fluorescent probe for STED microscopy to study NIP-specific B cells

ISSN
0003-2654
Date Issued
2021
Author(s)
Glowacki, Selda Kabatas
Yip, Ka Man
Asadpour, Ommolbanin
Münchhalfen, Matthias
Engels, Niklas
DOI
10.1039/D1AN00601K
Abstract
Monovalent NIP probes for studying B cell antigen receptors in fluorescence-based techniques, including diffraction unlimited microscopy.
We have developed a series of monovalent fluorophore-conjugated affinity probes based on the hapten 3-nitro-4-hydroxy-5-iodophenylacetyl (NIP), which is widely used as a model antigen to study B lymphocytes and the functional principles of B cell antigen receptors (BCRs). We successfully used them in flow-cytometry, confocal and super-resolution microscopy techniques.
Monovalent NIP probes for studying B cell antigen receptors in fluorescence-based techniques, including diffraction unlimited microscopy.
We have developed a series of monovalent fluorophore-conjugated affinity probes based on the hapten 3-nitro-4-hydroxy-5-iodophenylacetyl (NIP), which is widely used as a model antigen to study B lymphocytes and the functional principles of B cell antigen receptors (BCRs). We successfully used them in flow-cytometry, confocal and super-resolution microscopy techniques.