Gomes de Castro, Maria Angela

[["dc.bibliographiccitation.firstpage","4744"],["dc.bibliographiccitation.issue","15"],["dc.bibliographiccitation.journal","The Analyst"],["dc.bibliographiccitation.lastpage","4747"],["dc.bibliographiccitation.volume","146"],["dc.contributor.author","Glowacki, Selda Kabatas"],["dc.contributor.author","Gomes de Castro, Maria Angela"],["dc.contributor.author","Yip, Ka Man"],["dc.contributor.author","Asadpour, Ommolbanin"],["dc.contributor.author","Münchhalfen, Matthias"],["dc.contributor.author","Engels, Niklas"],["dc.contributor.author","Opazo, Felipe"],["dc.date.accessioned","2021-08-12T07:45:03Z"],["dc.date.available","2021-08-12T07:45:03Z"],["dc.date.issued","2021"],["dc.description.abstract","Monovalent NIP probes for studying B cell antigen receptors in fluorescence-based techniques, including diffraction unlimited microscopy."],["dc.description.abstract","We have developed a series of monovalent fluorophore-conjugated affinity probes based on the hapten 3-nitro-4-hydroxy-5-iodophenylacetyl (NIP), which is widely used as a model antigen to study B lymphocytes and the functional principles of B cell antigen receptors (BCRs). We successfully used them in flow-cytometry, confocal and super-resolution microscopy techniques."],["dc.description.abstract","Monovalent NIP probes for studying B cell antigen receptors in fluorescence-based techniques, including diffraction unlimited microscopy."],["dc.description.abstract","We have developed a series of monovalent fluorophore-conjugated affinity probes based on the hapten 3-nitro-4-hydroxy-5-iodophenylacetyl (NIP), which is widely used as a model antigen to study B lymphocytes and the functional principles of B cell antigen receptors (BCRs). We successfully used them in flow-cytometry, confocal and super-resolution microscopy techniques."],["dc.identifier.doi","10.1039/D1AN00601K"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/88359"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-448"],["dc.relation.eissn","1364-5528"],["dc.relation.issn","0003-2654"],["dc.title","A fluorescent probe for STED microscopy to study NIP-specific B cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","820"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Nature Communications"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Gomes de Castro, Maria Angela"],["dc.contributor.author","Wildhagen, Hanna"],["dc.contributor.author","Sograte-Idrissi, Shama"],["dc.contributor.author","Hitzing, Christoffer"],["dc.contributor.author","Binder, Mascha"],["dc.contributor.author","Trepel, Martin"],["dc.contributor.author","Engels, Niklas"],["dc.contributor.author","Opazo, Felipe"],["dc.date.accessioned","2019-07-09T11:50:13Z"],["dc.date.available","2019-07-09T11:50:13Z"],["dc.date.issued","2019"],["dc.description.abstract","Stimulation of the B cell antigen receptor (BCR) triggers signaling pathways that promote the differentiation of B cells into plasma cells. Despite the pivotal function of BCR in B cell activation, the organization of the BCR on the surface of resting and antigen-activated B cells remains unclear. Here we show, using STED super-resolution microscopy, that IgM-containing BCRs exist predominantly as monomers and dimers in the plasma membrane of resting B cells, but form higher oligomeric clusters upon stimulation. By contrast, a chronic lymphocytic leukemia-derived BCR forms dimers and oligomers in the absence of a stimulus, but a single amino acid exchange reverts its organization to monomers in unstimulated B cells. Our super-resolution microscopy approach for quantitatively analyzing cell surface proteins may thus help reveal the nanoscale organization of immunoreceptors in various cell types."],["dc.identifier.doi","10.1038/s41467-019-08677-1"],["dc.identifier.pmid","30778055"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15884"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59725"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","2041-1723"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","610"],["dc.title","Differential organization of tonic and chronic B cell antigen receptors in the plasma membrane"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","L15"],["dc.bibliographiccitation.firstpage","L15"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","The Astrophysical Journal Letters"],["dc.bibliographiccitation.volume","820"],["dc.contributor.author","do Nascimento, Jr., J.-D."],["dc.contributor.author","Vidotto, A. A."],["dc.contributor.author","Petit, P."],["dc.contributor.author","Folsom, C."],["dc.contributor.author","Castro, M."],["dc.contributor.author","Marsden, S. C."],["dc.contributor.author","Meibom, S."],["dc.contributor.author","Guinan, E."],["dc.contributor.author","Ribas, I."],["dc.contributor.author","Morin, Julien"],["dc.contributor.author","Porto de Mello, G. F."],["dc.contributor.author","Jeffers, Sandra V."],["dc.date.accessioned","2020-12-10T18:47:37Z"],["dc.date.available","2020-12-10T18:47:37Z"],["dc.date.issued","2016"],["dc.description.abstract","We report magnetic field measurements for kappa(1) Cet, a proxy of the young Sun when life arose on Earth. We carry out an analysis of the magnetic properties determined from spectropolarimetric observations and reconstruct the large-scale surface magnetic field to derive the magnetic environment, stellar winds, and particle flux permeating the interplanetary medium around kappa(1) Cet. Our results show a closer magnetosphere and mass-loss rate of M = 9.7 x 10(-13) M-circle dot yr(-1), i.e., a factor of 50 times larger than the current solar wind mass-loss rate, resulting in a larger interaction via space weather disturbances between the stellar wind and a hypothetical young-Earth analogue, potentially affecting the planet's habitability. Interaction of the wind from the young Sun with the planetary ancient magnetic field may have affected the young Earth and its life conditions."],["dc.identifier.doi","10.3847/2041-8205/820/1/L15"],["dc.identifier.eissn","2041-8213"],["dc.identifier.isi","000372352000015"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/78823"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.issn","2041-8213"],["dc.relation.issn","2041-8205"],["dc.title","MAGNETIC FIELD AND WIND OF KAPPA CETI: TOWARD THE PLANETARY HABITABILITY OF THE YOUNG SUN WHEN LIFE AROSE ON EARTH"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e0173050"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","de Castro, Maria Angela Gomes"],["dc.contributor.author","Hoebartner, Claudia"],["dc.contributor.author","Opazo, Felipe"],["dc.date.accessioned","2018-11-07T10:27:12Z"],["dc.date.available","2018-11-07T10:27:12Z"],["dc.date.issued","2017"],["dc.description.abstract","Continuous improvements in imaging techniques are challenging biologists to search for more accurate methods to label cellular elements. This is particularly relevant for diffraction-unlimited fluorescence imaging, where the perceived resolution is affected by the size of the affinity probes. This is evident when antibodies, which are 10-15 nm in size, are used. Previously it has been suggested that RNA aptamers (similar to 3 nm) can be used to detect cellular proteins under super-resolution imaging. However, a direct comparison between several aptamers and antibodies is needed, to clearly show the advantages and/or disadvantages of the different probes. Here we have conducted such a comparative study, by testing several aptamers and antibodies using stimulated emission depletion microscopy (STED). We have targeted three membrane receptors, EGFR, ErbB2 and Epha2, which are relevant to human health, and recycle between plasma membrane and intracellular organelles. Our results suggest that the aptamers can reveal more epitopes than most antibodies, thus providing a denser labeling of the stained structures. Moreover, this improves the overall quality of the information that can be extracted from the images. We conclude that aptamers could become useful fluorescent labeling tools for light microscopy and super-resolution imaging, and that their development for novel targets is imperative."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2017"],["dc.identifier.doi","10.1371/journal.pone.0173050"],["dc.identifier.isi","000394688200190"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14378"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/43201"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Aptamers provide superior stainings of cellular receptors studied under super-resolution microscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1542"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","ACS Chemical Neuroscience"],["dc.bibliographiccitation.lastpage","1551"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Agüi-Gonzalez, Paola"],["dc.contributor.author","Bao, Guobin"],["dc.contributor.author","Gomes de Castro, Maria Angela"],["dc.contributor.author","Rizzoli, Silvio O."],["dc.contributor.author","Phan, Nhu T. N."],["dc.date.accessioned","2021-06-01T09:41:35Z"],["dc.date.available","2021-06-01T09:41:35Z"],["dc.date.issued","2021"],["dc.description.abstract","The cellular functions of lipids in the neuronal plasma membranes have been increasingly acknowledged, particularly their association to neuronal processes and synaptic plasticity. However, the knowledge of their regulatory mechanisms in neuronal cells remains sparse. To address this, we investigated the lipid organization of the plasma membranes of hippocampal neurons in relation to neuronal activity using secondary ion mass spectrometry imaging. The neurons were treated with drugs, particularly tetrodotoxin (TTX) and bicuculline (BIC), to induce chronic activation and silencing. Distinct lipid organization was found in the plasma membrane of the cell body and the neurites. Moreover, significant alterations of the levels of the membrane lipids, especially ceramides, phosphatidylserines, phosphatidic acids, and triacylglycerols, were observed under the TTX and BIC treatments. We suggest that many types of membrane lipids are affected by, and may be involved in, the regulation of neuronal function."],["dc.identifier.doi","10.1021/acschemneuro.1c00031"],["dc.identifier.pmid","33896172"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/84972"],["dc.identifier.url","https://sfb1286.uni-goettingen.de/literature/publications/119"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation","SFB 1286: Quantitative Synaptologie"],["dc.relation.eissn","1948-7193"],["dc.relation.issn","1948-7193"],["dc.relation.workinggroup","RG Rizzoli (Quantitative Synaptology in Space and Time)"],["dc.rights","CC BY 4.0"],["dc.title","Secondary Ion Mass Spectrometry Imaging Reveals Changes in the Lipid Structure of the Plasma Membranes of Hippocampal Neurons following Drugs Affecting Neuronal Activity"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","17"],["dc.bibliographiccitation.journal","BIO-PROTOCOL"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Gomes de Castro, Maria Angela"],["dc.contributor.author","Höbartner, Claudia"],["dc.contributor.author","Opazo, Felipe"],["dc.date.accessioned","2020-12-10T18:43:03Z"],["dc.date.available","2020-12-10T18:43:03Z"],["dc.date.issued","2017"],["dc.identifier.doi","10.21769/BioProtoc.2541"],["dc.identifier.issn","2331-8325"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/78178"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Staining of Membrane Receptors with Fluorescently-labeled DNA Aptamers for Super-resolution Imaging"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]