Reply to "Polarization modulation adds little additional information to super-resolution fluorescence microscopy"

We are grateful to Keller et al. for the opportunity to clarify the role of the SPEED (sparsity penalty–enhanced estimation by demodulation) algorithm and the impact of the polarization information in SPoD (super-resolution by polarization demodulation). We concur with Keller et al. that for densely labeled and spatially sparse samples, the information gain from polarization is negligible in their reanalysis of our data, as they observed that nearly identical resolution can also be achieved by sparse deconvolution alone using the SPEED algorithm. However, this does not mean that no gain in resolution is possible with the modulation information alone.