A STED Microscope Designed for Routine Biomedical Applications

ISSN
1559-8985
Date Issued
2014
Author(s)
Görlitz, Frederik
Hoyer, Patrick
Falk, Henning J.
Engelhardt, Johann
DOI
10.2528/PIER14042708
Abstract
We present a multi-color STED fluorescence microscope providing far-field optical resolution down to 20 nm for biomedical research. The optical design comprises fiber lasers, beam scanners, and a set of active and passive polarizing elements that cooperatively yield an optically robust system for routinely imaging samples at subdiffraction length scales.