Kastrup, Lars

[["dc.bibliographiccitation.firstpage","3125"],["dc.bibliographiccitation.issue","18"],["dc.bibliographiccitation.journal","Applied Physics Letters"],["dc.bibliographiccitation.lastpage","3127"],["dc.bibliographiccitation.volume","82"],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Blanca, C. M."],["dc.contributor.author","Dyba, Marcus"],["dc.contributor.author","Kastrup, Lars"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:45:00Z"],["dc.date.available","2017-09-07T11:45:00Z"],["dc.date.issued","2003"],["dc.description.abstract","We report subdiffraction resolution in far-field fluorescence microscopy through laser-diode-stimulated emission depletion of molecular markers. The diode-generated focal intensities lead to a resolution improvement by similar to45% in both lateral directions. Excitation and stimulated emission are performed with electronically synchronized diode pulses of 50-70 ps and 300-400 ps duration, respectively. The subdiffraction resolution is utilized to resolve neighboring individual molecules."],["dc.identifier.doi","10.1063/1.1571656"],["dc.identifier.gro","3144107"],["dc.identifier.isi","000182570000063"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1695"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0003-6951"],["dc.title","Laser-diode-stimulated emission depletion microscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","6814"],["dc.bibliographiccitation.issue","48"],["dc.bibliographiccitation.journal","Angewandte Chemie"],["dc.bibliographiccitation.lastpage","6818"],["dc.bibliographiccitation.volume","116"],["dc.contributor.author","Kastrup, Lars"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:53:02Z"],["dc.date.available","2017-09-07T11:53:02Z"],["dc.date.issued","2004"],["dc.description.abstract","Lichtschalter: Der Photoneneinfangquerschnitt einzelner fluoreszierender Moleküle ist direkt messbar, indem man angeregte Moleküle mit Licht „ausschaltet“. Diese stimulierte Emission (siehe Abbildung) ermöglicht die direkte Manipulation des angeregten Zustandes eines einzelnen Moleküls bei Raumtemperatur."],["dc.identifier.doi","10.1002/ange.200461337"],["dc.identifier.gro","3145024"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2715"],["dc.language.iso","de"],["dc.notes.intern","Crossref Import"],["dc.notes.status","final"],["dc.notes.submitter","chake"],["dc.relation.issn","1521-3757"],["dc.title","Absolute optische Wirkungsquerschnitte fluoreszierender Einzelmoleküle"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3130"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Optics Express"],["dc.bibliographiccitation.lastpage","3143"],["dc.bibliographiccitation.volume","19"],["dc.contributor.author","Bückers, Johanna"],["dc.contributor.author","Wildanger, Dominik"],["dc.contributor.author","Vicidomini, Giuseppe"],["dc.contributor.author","Kastrup, Lars"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:44:21Z"],["dc.date.available","2017-09-07T11:44:21Z"],["dc.date.issued","2011"],["dc.description.abstract","We describe a STED microscope optimized for colocalization experiments with up to three colors. Two fluorescence labels are separated by their fluorescence lifetime whereas a third channel is discriminated by the wavelength of fluorescence emission. Since it does not require a second STED beam, separating by lifetime is insensitive to drift and thus optimally suited for colocalization analyses. Furthermore, we propose a setup having a second STED beam for long duration multicolor recording."],["dc.identifier.doi","10.1364/OE.19.003130"],["dc.identifier.gro","3142776"],["dc.identifier.isi","000288860000031"],["dc.identifier.pmid","21369135"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/217"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: European Community [201837]"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1094-4087"],["dc.title","Simultaneous multi-lifetime multi-color STED imaging for colocalization analyses"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","47"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Nachrichten aus der Chemie"],["dc.bibliographiccitation.lastpage","50"],["dc.bibliographiccitation.volume","55"],["dc.contributor.author","Hell, Stefan W."],["dc.contributor.author","Kastrup, Lars"],["dc.date.accessioned","2018-04-23T11:48:25Z"],["dc.date.available","2018-04-23T11:48:25Z"],["dc.date.issued","2007"],["dc.description.abstract","Obwohl das Stimulated‐Emission‐Depletion (STED)‐Fluoreszenzmikroskop Licht auf herkömmliche Weise fokussiert, ist die Auflösung nicht durch Beugung begrenzt und kann prinzipiell molekulare Dimensionen erreichen. Die zugrunde liegende Idee gilt für alle reversibel schaltbaren optischen Übergänge eines Markermoleküls. So wäre die Beugungsgrenze für optisches Abbilden und Strukturieren überwunden."],["dc.identifier.doi","10.1002/nadc.200744314"],["dc.identifier.gro","3142369"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/13508"],["dc.language.iso","en"],["dc.notes.intern","lifescience updates Crossref Import"],["dc.notes.status","final"],["dc.relation.issn","1439-9598"],["dc.title","Fluoreszenzmikroskopie sieht chemisch scharf"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","51"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Current Pharmaceutical Biotechnology"],["dc.bibliographiccitation.lastpage","56"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Blom, H."],["dc.contributor.author","Kastrup, L."],["dc.contributor.author","Eggeling, C."],["dc.date.accessioned","2018-03-19T22:41:14Z"],["dc.date.available","2018-03-19T22:41:14Z"],["dc.date.issued","2006"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/13089"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.title","Fluorescence spectroscopy in reduced detection volumes"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Abstracts of Papers of the American Chemical Society"],["dc.bibliographiccitation.volume","228"],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Dyba, Marcus"],["dc.contributor.author","Kastrup, Lars"],["dc.date.accessioned","2017-09-07T11:45:51Z"],["dc.date.available","2017-09-07T11:45:51Z"],["dc.date.issued","2004"],["dc.identifier.gro","3145545"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/3254"],["dc.notes.intern","lifescience"],["dc.notes.status","public"],["dc.notes.submitter","oschaef1"],["dc.relation.issn","0065-7727"],["dc.title","Fluorescence nanoscopy through reversible optically saturable transitions"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","57"],["dc.bibliographiccitation.journal","Progress In Electromagnetics Research"],["dc.bibliographiccitation.lastpage","68"],["dc.bibliographiccitation.volume","147"],["dc.contributor.author","Görlitz, Frederik"],["dc.contributor.author","Hoyer, Patrick"],["dc.contributor.author","Falk, Henning J."],["dc.contributor.author","Kastrup, Lars"],["dc.contributor.author","Engelhardt, Johann"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:46:54Z"],["dc.date.available","2017-09-07T11:46:54Z"],["dc.date.issued","2014"],["dc.description.abstract","We present a multi-color STED fluorescence microscope providing far-field optical resolution down to 20 nm for biomedical research. The optical design comprises fiber lasers, beam scanners, and a set of active and passive polarizing elements that cooperatively yield an optically robust system for routinely imaging samples at subdiffraction length scales."],["dc.identifier.doi","10.2528/PIER14042708"],["dc.identifier.gro","3142202"],["dc.identifier.isi","000350673700004"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/5665"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: Federal Ministry of Education and Research (BMBF) [FKZ: 13N11173]"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1559-8985"],["dc.title","A STED Microscope Designed for Routine Biomedical Applications"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","16100"],["dc.bibliographiccitation.issue","18"],["dc.bibliographiccitation.journal","Optics Express"],["dc.bibliographiccitation.lastpage","16110"],["dc.bibliographiccitation.volume","17"],["dc.contributor.author","Wildanger, Dominik"],["dc.contributor.author","Bückers, Johanna"],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Kastrup, Lars"],["dc.date.accessioned","2017-09-07T11:46:52Z"],["dc.date.available","2017-09-07T11:46:52Z"],["dc.date.issued","2009"],["dc.description.abstract","STED microscopes are commonly built using separate optical paths for the excitation and the STED beam. As a result, the beams must be co-aligned and can be subject to mechanical drift. Here, we present a single-path STED microscope whose beams are aligned by design and hence is insensitive to mechanical drift. The design of a phase plate is described which selectively modulates the STED beam but leaves the excitation beam unaffected. The performance of the single-beam setup is on par with previous dual-beam designs."],["dc.identifier.doi","10.1364/OE.17.016100"],["dc.identifier.gro","3143069"],["dc.identifier.isi","000269781700074"],["dc.identifier.pmid","19724610"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/542"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: European Community [201837]; German National Academic Foundation"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1094-4087"],["dc.title","A STED microscope aligned by design"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","644"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Microscopy Research and Technique"],["dc.bibliographiccitation.lastpage","650"],["dc.bibliographiccitation.volume","71"],["dc.contributor.author","Punge, Annedore"],["dc.contributor.author","Rizzoli, Silvio"],["dc.contributor.author","Jahn, Reinhard"],["dc.contributor.author","Wildanger, Jan Dominik"],["dc.contributor.author","Meyer, Lars"],["dc.contributor.author","Schönle, Andreas"],["dc.contributor.author","Kastrup, Lars"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:48:14Z"],["dc.date.available","2017-09-07T11:48:14Z"],["dc.date.issued","2008"],["dc.description.abstract","Tackling biological problems often involves the imaging and localization of cellular structures on the nanometer scale. Although optical super-resolution below 100 nm can be readily attained with stimulated emission depletion (STED) and photoswitching microscopy methods, attaining an axial resolution <100 nm with focused light generally required the use of two lenses in a 4Pi configuration or exceptionally bright photochromic fluorophores. Here, we describe a simple technical solution for 3D nanoscopy of fixed samples: biological specimens are fluorescently labeled, embedded in a polymer resin, cut into thin sections, and then imaged via STED microscopy with nanoscale resolution. This approach allows a 3D image reconstruction with a resolution <80 nm in all directions using available state-of-the art STED microscopes."],["dc.identifier.doi","10.1002/jemt.20602"],["dc.identifier.gro","3143247"],["dc.identifier.isi","000259533900003"],["dc.identifier.pmid","18512740"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/740"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: Human Frontier Science Program"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1059-910X"],["dc.title","3D reconstruction of high-resolution STED microscope images"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","9614"],["dc.bibliographiccitation.issue","13"],["dc.bibliographiccitation.journal","Optics Express"],["dc.bibliographiccitation.lastpage","9621"],["dc.bibliographiccitation.volume","16"],["dc.contributor.author","Wildanger, Dominik"],["dc.contributor.author","Rittweger, Eva"],["dc.contributor.author","Kastrup, Lars"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:48:17Z"],["dc.date.available","2017-09-07T11:48:17Z"],["dc.date.issued","2008"],["dc.description.abstract","We report on a straightforward yet powerful implementation of stimulated emission depletion (STED) fluorescence microscopy providing subdiffraction resolution in the far-field. Utilizing the same super-continuum pulsed laser source both for excitation and STED, this implementation of STED microscopy avoids elaborate preparations of laser pulses and conveniently provides multicolor imaging. Operating at pulse repetition rates around 1 MHz, it also affords reduced photobleaching rates by allowing the fluorophore to relax from excitable metastable dark states involved in photodegradation. The imaging of dense nanoparticles and of the microtubular network of mammalian cells evidences a spatial resolution of 30-50 nm in the focal plane, i.e. by a factor of 8-9 beyond the diffraction barrier."],["dc.identifier.doi","10.1364/OE.16.009614"],["dc.identifier.gro","3143277"],["dc.identifier.isi","000257563900041"],["dc.identifier.pmid","18575529"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/773"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1094-4087"],["dc.title","STED microscopy with a supercontinuum laser source"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]