Now showing 1 - 10 of 23
  • 2014Journal Article Research Paper
    [["dc.bibliographiccitation.artnumber","088102"],["dc.bibliographiccitation.firstpage","3"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Physical Review Letters"],["dc.bibliographiccitation.lastpage","7"],["dc.bibliographiccitation.volume","112"],["dc.contributor.author","Weinhausen, Britta"],["dc.contributor.author","Saldanha, Oliva"],["dc.contributor.author","Wilke, Robin N."],["dc.contributor.author","Dammann, Christian"],["dc.contributor.author","Priebe, Marius"],["dc.contributor.author","Burghammer, Manfred"],["dc.contributor.author","Sprung, Michael"],["dc.contributor.author","Köster, Sarah"],["dc.date.accessioned","2017-09-07T11:46:29Z"],["dc.date.available","2017-09-07T11:46:29Z"],["dc.date.issued","2014"],["dc.description.abstract","High-resolution x-ray imaging techniques offer a variety of possibilities for studying the nanoscale structure of biological cells. A challenging task remains the study of cells by x rays in their natural, aqueous environment. Here, we overcome this limitation by presenting scanning x-ray diffraction measurements with beam sizes in the range of a few hundred nm on living and fixed-hydrated eukaryotic cells in microfluidic devices which mimic a native environment. The direct comparison between fixed-hydrated and living cells shows distinct differences in the scattering signal, pointing to structural changes on the order of 30 to 50 nm."],["dc.identifier.doi","10.1103/PhysRevLett.112.088102"],["dc.identifier.gro","3142182"],["dc.identifier.isi","000331957600012"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/5443"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1079-7114"],["dc.relation.issn","0031-9007"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Köster (Cellular Biophysics)"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","x-ray scattering"],["dc.subject.gro","cellular biophysics"],["dc.subject.gro","microfluidics"],["dc.title","Scanning X-Ray Nanodiffraction on Living Eukaryotic Cells in Microfluidic Environments"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]
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  • 2019Journal Article
    [["dc.bibliographiccitation.artnumber","3034"],["dc.bibliographiccitation.firstpage","3034"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Holme, Hans Christian Martin"],["dc.contributor.author","Rosenzweig, Sebastian"],["dc.contributor.author","Ong, Frank"],["dc.contributor.author","Wilke, Robin Niklas"],["dc.contributor.author","Lustig, Michael"],["dc.contributor.author","Uecker, Martin"],["dc.date.accessioned","2019-07-09T11:50:05Z"],["dc.date.accessioned","2020-05-13T11:05:11Z"],["dc.date.available","2019-07-09T11:50:05Z"],["dc.date.available","2020-05-13T11:05:11Z"],["dc.date.issued","2019"],["dc.description.abstract","Robustness against data inconsistencies, imaging artifacts and acquisition speed are crucial factors limiting the possible range of applications for magnetic resonance imaging (MRI). Therefore, we report a novel calibrationless parallel imaging technique which simultaneously estimates coil profiles and image content in a relaxed forward model. Our method is robust against a wide class of data inconsistencies, minimizes imaging artifacts and is comparably fast, combining important advantages of many conceptually different state-of-the-art parallel imaging approaches. Depending on the experimental setting, data can be undersampled well below the Nyquist limit. Here, even high acceleration factors yield excellent imaging results while being robust to noise and the occurrence of phase singularities in the image domain, as we show on different data. Moreover, our method successfully reconstructs acquisitions with insufficient field-of-view. We further compare our approach to ESPIRiT and SAKE using spin-echo and gradient echo MRI data from the human head and knee. In addition, we show its applicability to non-Cartesian imaging on radial FLASH cardiac MRI data. Using theoretical considerations, we show that ENLIVE can be related to a low-rank formulation of blind multi-channel deconvolution, explaining why it inherently promotes low-rank solutions."],["dc.identifier.doi","10.1038/s41598-019-39888-7"],["dc.identifier.pmid","30816312"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15854"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59698"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/65304"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","2045-2322"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","610"],["dc.title","ENLIVE: An Efficient Nonlinear Method for Calibrationless and Robust Parallel Imaging"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]
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  • 2015Journal Article Research Paper
    [["dc.bibliographiccitation.firstpage","1986"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","1995"],["dc.bibliographiccitation.volume","109"],["dc.contributor.author","Giewekemeyer, Klaus"],["dc.contributor.author","Hackenberg, C."],["dc.contributor.author","Aquila, Andrew"],["dc.contributor.author","Wilke, Robin Niklas"],["dc.contributor.author","Groves, M. R."],["dc.contributor.author","Jordanova, R."],["dc.contributor.author","Lamzin, V. S."],["dc.contributor.author","Borchers, G."],["dc.contributor.author","Saksl, K."],["dc.contributor.author","Zozulya, Alla L."],["dc.contributor.author","Sprung, M."],["dc.contributor.author","Mancuso, A. P."],["dc.date.accessioned","2018-11-07T09:49:05Z"],["dc.date.available","2018-11-07T09:49:05Z"],["dc.date.issued","2015"],["dc.description.abstract","The structural investigation of noncrystalline, soft biological matter using x-rays is of rapidly increasing interest. Large-scale x-ray sources, such as synchrotrons and x-ray free electron lasers, are becoming ever brighter and make the study of such weakly scattering materials more feasible. Variants of coherent diffractive imaging (CDI) are particularly attractive, as the absence of an objective lens between sample and detector ensures that no x-ray photons scattered by a sample are lost in a limited-efficiency imaging system. Furthermore, the reconstructed complex image contains quantitative density information, most directly accessible through its phase, which is proportional to the projected electron density of the sample. If applied in three dimensions, CDI can thus recover the sample's electron density distribution. As the extension to three dimensions is accompanied by a considerable dose applied to the sample, cryogenic cooling is necessary to optimize the structural preservation of a unique sample in the beam. This, however, imposes considerable technical challenges on the experimental realization. Here, we show a route toward the solution of these challenges using ptychographic CDI (PCDI), a scanning variant of coherent imaging. We present an experimental demonstration of the combination of three-dimensional structure determination through PCDI with a cryogenically cooled biological sample-a budding yeast cell (Saccharomyces cerevisiae)-using hard (7.9 keV) synchrotron x-rays. This proof-of-principle demonstration in particular illustrates the potential of PCDI for highly sensitive, quantitative three-dimensional density determination of cryogenically cooled, hydrated, and unstained biological matter and paves the way to future studies of unique, nonreproducible biological cells at higher resolution."],["dc.identifier.doi","10.1016/j.bpj.2015.08.047"],["dc.identifier.isi","000364009200024"],["dc.identifier.pmid","26536275"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12679"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/35439"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation","SFB 755: Nanoscale Photonic Imaging"],["dc.relation.issn","1542-0086"],["dc.relation.issn","0006-3495"],["dc.relation.orgunit","Fakultät für Physik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights","CC BY-NC-ND 4.0"],["dc.rights.uri","http://creativecommons.org/licenses/by-nc-nd/4.0/"],["dc.subject.gro","x-ray optics and imaging"],["dc.title","Tomography of a Cryo-immobilized Yeast Cell Using Ptychographic Coherent X-Ray Diffractive Imaging"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]
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  • 2015Thesis
    [["dc.bibliographiccitation.seriesnr","14"],["dc.contributor.author","Wilke, Robin Niklas"],["dc.date.accessioned","2018-05-03T10:44:30Z"],["dc.date.available","2018-05-03T10:44:30Z"],["dc.date.issued","2015"],["dc.description.abstract","Since its first experimental demonstration in 1999, Coherent X-Ray Diffractive Imaging has become one of the most promising high resolution X-Ray imaging techniques using coherent radiation produced by brilliant synchrotron storage rings. The ability to directly invert diffraction data with the help of advanced algorithms has paved the way for microscopic investigations and wave-field analyses on the spatial scale of nanometres without the need for inefficient imaging lenses. X-Ray phase contrast which is a measure of the electron density is an important contrast mode of soft biological specimens. For the case of many dominant elements of soft biological matter, the electron density can be converted into an effective mass density offering a unique quantitative information channel which may shed light on important questions such as DNA compaction in the bacterial nucleoid through ‚weighing with light‘. In this work X-Ray phase contrast maps have been obtained from different biological samples by exploring different methods. In particular, the techniques Ptychography and Waveguide-Holographic-Imaging have been used to obtain twodimensional and three-dimensional mass density maps on the single-cell-level of freeze-dried cells of the bacteria Deinococcus radiodurans, Bacillus subtilis and Bacillus thuringiensis allowing, for instance, to estimate the dry weight of the bacterial genome in a near native state. On top of this, reciprocal space information from coherent small angle X-Ray scattering (cellular Nano-Diffraction) of the fine structure of the bacterial cells has been recorded in a synergistic manner and has been analysed down to a resolution of about 2.3/nm exceeding current limits of direct imaging approaches. Furthermore, the dynamic range of present detector technology being one of the major limiting factors of ptychographic phasing of farfield diffraction data has been significantly increased. Overcoming this problem for the case of the very intense X-Ray beam produced by Kirkpatrick-Baez mirrors has been explored by using semi-transparent central stops."],["dc.format.extent","VI, 238"],["dc.identifier.doi","10.17875/gup2015-792"],["dc.identifier.isbn","978-3-86395-190-0"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?isbn-978-3-86395-190-0"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/14386"],["dc.identifier.urn","urn:nbn:de:gbv:7-isbn-978-3-86395-190-0-2"],["dc.language.iso","en"],["dc.notes.intern","TASK GROB-550"],["dc.notes.status","Dissertation"],["dc.publisher","Universitätsverlag Göttingen"],["dc.publisher.place","Göttingen"],["dc.relation.crisseries","Göttingen Series in X-Ray Physics"],["dc.relation.ispartofseries","Göttingen Series in X-ray Physics; 14"],["dc.rights","CC BY-SA 4.0"],["dc.rights.uri","http://creativecommons.org/licenses/by-sa/4.0/"],["dc.title","Coherent X-ray diffractive imaging on the single-cell-level of microbial samples"],["dc.title.subtitle","ptychography, tomography, nano-diffraction and waveguide-imaging"],["dc.type","thesis"],["dc.type.internalPublication","unknown"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]
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  • 2015Conference Paper
    [["dc.contributor.author","Osterhoff, Markus"],["dc.contributor.author","Döring, Florian"],["dc.contributor.author","Eberl, Christian"],["dc.contributor.author","Wilke, Robin N."],["dc.contributor.author","Wallentin, Jesper"],["dc.contributor.author","Krebs, Hans-Ulrich"],["dc.contributor.author","Sprung, Michael"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2017-09-07T11:54:07Z"],["dc.date.available","2017-09-07T11:54:07Z"],["dc.date.issued","2015"],["dc.identifier.doi","10.1117/12.2187799"],["dc.identifier.gro","3145110"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2810"],["dc.notes.intern","Crossref Import"],["dc.notes.status","public"],["dc.publisher","SPIE"],["dc.publisher.place","Bellingham, Wash."],["dc.relation","SFB 755: Nanoscale Photonic Imaging"],["dc.relation.conference","X-ray nanoimaging: instruments and methods"],["dc.relation.eventend","2015-08-13"],["dc.relation.eventlocation","San Diego, Calif."],["dc.relation.eventstart","2015-08-12"],["dc.relation.isbn","978-1-62841-758-6"],["dc.relation.ispartof","X-ray nanoimaging: instruments and methods II: 12 - 13 August 2015, San Diego, California, United States"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray optics"],["dc.subject.gro","x-ray imaging"],["dc.title","Progress on multi-order hard x-ray imaging with multilayer zone plates"],["dc.type","conference_paper"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]
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  • 2015Journal Article Research Paper
    [["dc.bibliographiccitation.firstpage","116"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Journal of Applied Crystallography"],["dc.bibliographiccitation.lastpage","124"],["dc.bibliographiccitation.volume","48"],["dc.contributor.author","Osterhoff, Markus"],["dc.contributor.author","Eberl, Christian"],["dc.contributor.author","Döring, Florian"],["dc.contributor.author","Wilke, Robin N."],["dc.contributor.author","Wallentin, Jesper"],["dc.contributor.author","Krebs, Hans Ulrich"],["dc.contributor.author","Sprung, Michael"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2020-11-05T15:05:24Z"],["dc.date.available","2020-11-05T15:05:24Z"],["dc.date.issued","2015"],["dc.description.abstract","This article describes holographic imaging experiments using a hard X-ray multilayer zone plate (MZP) with an outermost zone width of 10nm at a photon energy of 18keV. An order-sorting aperture (OSA) is omitted and emulated during data analysis by a 'software OSA'. Scanning transmission X-ray microscopy usually carried out in the focal plane is generalized to the holographic regime. The MZP focus is characterized by a three-plane phase-retrieval algorithm to an FWHM of 10nm."],["dc.identifier.doi","10.1107/S1600576714026016"],["dc.identifier.gro","3141965"],["dc.identifier.isi","000349210700016"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13802"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/68460"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-352.6"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation","SFB 755: Nanoscale Photonic Imaging"],["dc.relation.eissn","1600-5767"],["dc.relation.issn","1600-5767"],["dc.relation.orgunit","Institut für Materialphysik"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights.uri","https://goedoc.uni-goettingen.de/licenses"],["dc.subject.gro","x-ray optics"],["dc.subject.gro","x-ray imaging"],["dc.title","Towards multi-order hard X-ray imaging with multilayer zone plates"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]
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  • 2017Journal Article
    [["dc.bibliographiccitation.issue","suppl_1"],["dc.bibliographiccitation.journal","European Heart Journal"],["dc.bibliographiccitation.volume","38"],["dc.contributor.author","Unterberg-Buchwald, Christina"],["dc.contributor.author","Ritter, Christian Oliver"],["dc.contributor.author","Reupke, V."],["dc.contributor.author","Wilke, Robin Niklas"],["dc.contributor.author","Steinmetz, Michael"],["dc.contributor.author","Schuster, Andreas"],["dc.contributor.author","Lotz, Joachim"],["dc.contributor.author","Uecker, Martin"],["dc.date.accessioned","2020-05-13T13:45:40Z"],["dc.date.available","2020-05-13T13:45:40Z"],["dc.date.issued","2017"],["dc.description.abstract","Background: Endomyocardial biopsies (EMB) are an important diagnostic tool for myocarditis. Despite procedural success, the large sampling error results in the necessity of multiple (>6) biopsies. In cardiac magnetic resonance (CMR) imaging late gadolinium enhancement (LGE) depicts areas of affected myocardium. Thus, targeted biopsy under real-time magnetic resonance image guidance might reduce sampling error. Methods: Seven minipigs (MP) of the Goettingen strain underwent radiofrequency (RF) (2x30s, max. 30 W, temperature 60–64 °C) ablation in the left ventricle. Two focal lesions were induced (lateral wall in five apex in two animals). Biopsies were taken immediately after lesion induction using a 7 F conventional bioptome under fluoroscopic guidance (FLG) at the ablation site. Afterwards the CMR and lesion visualization by LGE was performed on a 3T MRI scanner. The lesions were biopsied under CMR-guidance using a MR-compatible bioptome (fig.1) guided by a steerable catheter. Interactive real-time visualization of the intervention was based on radial FLASH with nonlinear inverse reconstruction (NLINV) (temporal resolution 42 ms). All samples underwent a standard histological evaluation. Results: RF-ablation was successful in all MP. FL- guided biopsies were performed succesfully in 6/6 MP. Detection of RF lesions by CMR detection was successful in 7/7 MP, i.e. at least one lesion was clearly visible. Localization and tracking of the catheters and the bioptome using interactive control of the imaging plane was achieved in 6/6 MP; however in the MP with a large PE after EMB under fluoroscopy no further EMB was attempted for safety reasons. Biopsies under CMR guidance were successfully performed in 5/6 animals, in one MP the bioptome reached the lesion, however the forceps did not cut out a sample. Specimens obtained under CMR guidance contained part of the lesion in 6/15 (40%) myocardial specimens and in 4/5 (80%) animals in which samples were achieved. Conventional biopsies revealed ablation lesions in 4/17 (23.5%) specimens in 3/6 MP (50%). Conclusion: RF-induced focal lesions are a useful tool for CMR-guided biopsy studies in minipigs. In contrast to fluoroscopy, CMR provides excellent visualization of lesions. Interactive real-time MRI allows excellent passive tracking of the instruments and EMB provides significantly superior sampling accuracy compared to FL-guided biopsies. Improvements of MR-compatible bioptomes and guiding catheters are essential before applying this method in a clinical setting."],["dc.identifier.doi","10.1093/eurheartj/ehx502.P1428"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/65373"],["dc.language.iso","en"],["dc.relation.eissn","1522-9645"],["dc.relation.issn","0195-668X"],["dc.title","P1428Real time guidance for targeted endomyocardial biopsy in a minipig model"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]
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  • 2015Journal Article Research Paper
    [["dc.bibliographiccitation.firstpage","464"],["dc.bibliographiccitation.journal","Journal of Applied Crystallography"],["dc.bibliographiccitation.lastpage","476"],["dc.bibliographiccitation.volume","48"],["dc.contributor.author","Wilke, Robin N."],["dc.contributor.author","Hoppert, Michael"],["dc.contributor.author","Krenkel, Martin"],["dc.contributor.author","Bartels, Matthias"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2017-09-07T11:44:29Z"],["dc.date.available","2017-09-07T11:44:29Z"],["dc.date.issued","2015"],["dc.description.abstract","Quantitative waveguide-based X-ray phase contrast imaging has been carried out on the level of single, unstained, unsliced and freeze-dried bacterial cells of Bacillus thuringiensis and Bacillus subtilis using hard X-rays of 7.9keV photon energy. The cells have been prepared in the metabolically dormant state of an endospore. The quantitative phase maps obtained by iterative phase retrieval using a modified hybrid input-output algorithm allow for mass and mass density determinations on the level of single individual endospores but include also large field of view investigations. Additionally, a direct reconstruction based on the contrast transfer function is investigated, and the two approaches are compared. Depending on the field of view and method, a resolution down to 65nm was achieved at a maximum applied dose of below 5 x 10(5)Gy. Masses in the range of about approximate to 110-190(20)fg for isolated endospores have been obtained."],["dc.identifier.doi","10.1107/S1600576715003593"],["dc.identifier.fs","615792"],["dc.identifier.gro","3141933"],["dc.identifier.isi","000352229100017"],["dc.identifier.pmid","25844079"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13672"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2691"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.intern","Merged from goescholar"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1600-5767"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.orgunit","Fakultät für Physik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.gro","x-ray optics"],["dc.subject.gro","x-ray imaging"],["dc.title","Quantitative X-ray phase contrast waveguide imaging of bacterial endospores"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]
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  • 2015Journal Article Research Paper
    [["dc.bibliographiccitation.firstpage","380"],["dc.bibliographiccitation.issue","3-4"],["dc.bibliographiccitation.journal","Geomicrobiology Journal"],["dc.bibliographiccitation.lastpage","393"],["dc.bibliographiccitation.volume","32"],["dc.contributor.author","Wilke, Robin N."],["dc.contributor.author","Quéric, Nadia Valérie"],["dc.contributor.author","Hoppert, Michael"],["dc.contributor.author","Heller, C."],["dc.contributor.author","Schropp, A."],["dc.contributor.author","Schroer, C. G."],["dc.contributor.author","Burghammer, Manfred"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","Reitner, Joachim"],["dc.date.accessioned","2017-09-07T11:44:30Z"],["dc.date.available","2017-09-07T11:44:30Z"],["dc.date.issued","2015"],["dc.description.abstract","Modern scanning X-ray microscopy can help to unravel the spatial context between biotic and abiotic compounds of geobiological assemblies with the aim to finally link chemical pathways to biological activities at the nanometre scale. This work presents some multi-modal imaging techniques provided by hard X-ray microscopes at synchrotron radiation sources to address analytical needs in geobiological research. Using the examples of 1\\) a calcified basal skeleton of the demosponge Astrosclera willeyana, 2\\) an anaerobic methane-oxidizing microbial mat and 3\\) a bacterial sulfur-oxidizing consortium, we illustrate the potential of scanning X-ray fluorescence and scanning transmission X-ray microscopy, and a novel quantitative approach of ptychographic imaging at single cell level."],["dc.identifier.doi","10.1080/01490451.2014.908982"],["dc.identifier.gro","3141938"],["dc.identifier.isi","000352349600016"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2746"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1521-0529"],["dc.relation.issn","0149-0451"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","x-ray scattering"],["dc.title","Scanning Hard X-ray Microscopy Imaging Modalities for Geobiological Samples"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]
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  • 2015Journal Article Research Paper
    [["dc.bibliographiccitation.firstpage","1818"],["dc.bibliographiccitation.journal","Journal of Applied Crystallography"],["dc.bibliographiccitation.lastpage","1826"],["dc.bibliographiccitation.volume","48"],["dc.contributor.author","Wallentin, Jesper"],["dc.contributor.author","Wilke, Robin N."],["dc.contributor.author","Osterhoff, Markus"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2017-09-07T11:54:51Z"],["dc.date.available","2017-09-07T11:54:51Z"],["dc.date.issued","2015"],["dc.description.abstract","Simultaneous scanning Bragg contrast and small-angle ptychographic imaging of a single solar cell nanowire are demonstrated, using a nanofocused hard X-ray beam and two detectors. The 2.5 mu m-long nanowire consists of a single-crystal InP core of 190 nm diameter, coated with amorphous SiO2 and polycrystalline indium tin oxide. The nanowire was selected and aligned in real space using the small-angle scattering of the 140 x 210 nm X-ray beam. The orientation of the nanowire, as observed in small-angle scattering, was used to find the correct rotation for the Bragg condition. After alignment in real space and rotation, high-resolution (50 nm step) raster scans were performed to simultaneously measure the distribution of small-angle scattering and Bragg diffraction in the nanowire. Ptychographic reconstruction of the coherent small-angle scattering was used to achieve sub-beam spatial resolution. The small-angle scattering images, which are sensitive to the shape and the electron density of all parts of the nanowire, showed a homogeneous profile along the nanowire axis except at the thicker head region. In contrast, the scanning Bragg diffraction microscopy, which probes only the single-crystal InP core, revealed bending and crystalline inhomogeneity. Both systematic and non-systematic real-space movement of the nanowire were observed as it was rotated, which would have been difficult to reveal only from the Bragg scattering. These results demonstrate the advantages of simultaneously collecting and analyzing the small-angle scattering in Bragg diffraction experiments."],["dc.identifier.doi","10.1107/S1600576715017975"],["dc.identifier.gro","3141777"],["dc.identifier.isi","000365774800026"],["dc.identifier.pmid","26664342"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12753"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/957"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: K.A. Wallenberg Foundation; Deutsche Forschungsgemeinschaft [SFB 755]"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1600-5767"],["dc.relation.orgunit","Fakultät für Physik"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","x-ray scattering"],["dc.title","Simultaneous high-resolution scanning Bragg contrast and ptychographic imaging of a single solar cell nanowire"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]
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