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Priebe, Marius
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Priebe, Marius
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Priebe, Marius
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Priebe, M.
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2014Journal Article Research Paper [["dc.bibliographiccitation.artnumber","088102"],["dc.bibliographiccitation.firstpage","3"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Physical Review Letters"],["dc.bibliographiccitation.lastpage","7"],["dc.bibliographiccitation.volume","112"],["dc.contributor.author","Weinhausen, Britta"],["dc.contributor.author","Saldanha, Oliva"],["dc.contributor.author","Wilke, Robin N."],["dc.contributor.author","Dammann, Christian"],["dc.contributor.author","Priebe, Marius"],["dc.contributor.author","Burghammer, Manfred"],["dc.contributor.author","Sprung, Michael"],["dc.contributor.author","Köster, Sarah"],["dc.date.accessioned","2017-09-07T11:46:29Z"],["dc.date.available","2017-09-07T11:46:29Z"],["dc.date.issued","2014"],["dc.description.abstract","High-resolution x-ray imaging techniques offer a variety of possibilities for studying the nanoscale structure of biological cells. A challenging task remains the study of cells by x rays in their natural, aqueous environment. Here, we overcome this limitation by presenting scanning x-ray diffraction measurements with beam sizes in the range of a few hundred nm on living and fixed-hydrated eukaryotic cells in microfluidic devices which mimic a native environment. The direct comparison between fixed-hydrated and living cells shows distinct differences in the scattering signal, pointing to structural changes on the order of 30 to 50 nm."],["dc.identifier.doi","10.1103/PhysRevLett.112.088102"],["dc.identifier.gro","3142182"],["dc.identifier.isi","000331957600012"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/5443"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1079-7114"],["dc.relation.issn","0031-9007"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Köster (Cellular Biophysics)"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","x-ray scattering"],["dc.subject.gro","cellular biophysics"],["dc.subject.gro","microfluidics"],["dc.title","Scanning X-Ray Nanodiffraction on Living Eukaryotic Cells in Microfluidic Environments"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]Details DOI WOS2001Journal Article [["dc.bibliographiccitation.firstpage","76"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","PSYCHOPHARMAKOTHERAPIE"],["dc.bibliographiccitation.lastpage","80"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Holzinger, A."],["dc.contributor.author","Loffler, W."],["dc.contributor.author","Matschinger, H."],["dc.contributor.author","Mueller, P."],["dc.contributor.author","Priebe, S."],["dc.contributor.author","Angermeyer, M. C."],["dc.date.accessioned","2018-11-07T09:08:53Z"],["dc.date.available","2018-11-07T09:08:53Z"],["dc.date.issued","2001"],["dc.description.abstract","Results of representative survey in Germany have shown that the lay public takes a critical stance towards the treatment of mental disorders with psychotropic medication. This raises the question as to whether this view is shared by the schizophrenic patients. Method: Using a questionnaire consisting of ten 5-point Likert-scaled items. 104 schizophrenic patients, who were treated with clozapine. were asked to assess the positive and negative effects of psychotropic drugs. Follow-up?, were carried out 3 and 6 months later. Data from a representative survey of the German adult population a ere consulted fur comparison. Results: As compared with the general public. schizophrenic patients were more frequently prepared to acknowledge positive effects of psychotropic drugs. On the other hand. they less frequently subscribed to negative evaluations of the medication. Their assessment remained remarkably stable over the six-month period studied. Conclusion: While schizophrenic patients' evaluations of psychotropic drugs were less negative than those prevalent among the lay public. they also expressed a considerable amount of scepticism and insecurity."],["dc.identifier.isi","000168898000005"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/26135"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wissenschaftliche Verlag Mbh"],["dc.relation.issn","0944-6877"],["dc.title","Attitudes towards psychotropic drugs: schizophrenic patients vs. general public"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]Details WOS2002Journal Article [["dc.bibliographiccitation.firstpage","597"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","The Journal of Nervous and Mental Disease"],["dc.bibliographiccitation.lastpage","603"],["dc.bibliographiccitation.volume","190"],["dc.contributor.author","Holzinger, A."],["dc.contributor.author","Loffler, W."],["dc.contributor.author","Mueller, P."],["dc.contributor.author","Priebe, S."],["dc.contributor.author","Angermeyer, M. C."],["dc.date.accessioned","2018-11-07T10:06:30Z"],["dc.date.available","2018-11-07T10:06:30Z"],["dc.date.issued","2002"],["dc.description.abstract","This study investigates subjective illness theories of patients with schizophrenia, i.e., how they define their health problem, what they assume causes their illness and which course of illness they expect. The predictive value of those theories for patients' compliance with antipsychotic medication is tested. A problem-centered interview was conducted with 77 schizophrenic patients at discharge from inpatient or day hospital treatment. All patients were on clozapine treatment. Interviews were analyzed by means of computer-assisted content analysis. In addition, potential determinants of compliance were assessed using the 9th version of the Present State Examination, the UKU side effect rating scale, a checklist for patients' evaluations of the effect of psychotropic drugs, and a helping alliance scale. Compliance with medication was assessed by interviewing patients at discharge and three months later. Only slightly more than one half of the patients considered themselves mentally ill. They tended to endorse psychosocial causes more frequently as compared with biological causes. Slightly more than 25% of the patients each expected an improvement of the illness, a reoccurrence of the acute psychosis, or a chronic course. Whereas the quality of the helping alliance, delusion of grandiosity, and attitude toward psychotropic drugs proved to have an influence on patients' compliance with antipsychotic treatment, the three components of subjective illness theory (definition as mental illness, assumed etiology, and prognosis) did not have a statistically significant influence. Subjective illness theories vary in patients with schizophrenia. Although they might reflect different styles of coping with the illness, there is no evidence that they directly determine compliance with medication. Patients' views of the helping alliance and attitudes toward drugs should be considered in predicting compliance with antipsychotic medication."],["dc.identifier.doi","10.1097/00005053-200209000-00004"],["dc.identifier.isi","000178402600004"],["dc.identifier.pmid","12357093"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/39104"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Lippincott Williams & Wilkins"],["dc.relation.issn","0022-3018"],["dc.title","Subjective illness theory and antipsychotic medication compliance by patients with schizophrenia"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]Details DOI PMID PMC WOS2012Journal Article Research Paper [["dc.bibliographiccitation.firstpage","85"],["dc.bibliographiccitation.journal","Journal of Applied Crystallography"],["dc.bibliographiccitation.lastpage","92"],["dc.bibliographiccitation.volume","45"],["dc.contributor.author","Krywka, Christina"],["dc.contributor.author","Neubauer, Henrike"],["dc.contributor.author","Priebe, Marius"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","Keckes, Jozef"],["dc.contributor.author","Buffet, Adeline"],["dc.contributor.author","Roth, Stephan Volkher"],["dc.contributor.author","Doehrmann, Ralph"],["dc.contributor.author","Mueller, Martin"],["dc.date.accessioned","2017-09-07T11:49:00Z"],["dc.date.available","2017-09-07T11:49:00Z"],["dc.date.issued","2012"],["dc.description.abstract","The micro- and nanofocus X-ray scattering (MINAXS) beamline of PETRA III is equipped with two consecutively arranged endstations, the last of which is the nanofocus endstation. The first in-beam commissioning of the experimental equipment was successfully performed at the end of 2010, using two-dimensionally confining hard X-ray silicon waveguides with cross sections of 50 nm x 50 mu m to 50 nm x 2 mu m for nanobeam generation. A full characterization of the waveguide-generated beams was performed, giving values for the beam geometries, the transmission efficiencies of the waveguides and absolute fluxes. Along with these results a detailed description of the setup is presented in this paper. A first high-resolution nanodiffraction experiment on a nanocrystalline TiN hard coating was performed to verify the resolution of the nanodiffraction setup and to reveal the local gradients across the blasted TiN coating. In conclusion, the main concern is the availability of the nanobeam, how it was generated and the fact that a beam out of a two-dimensionally confining waveguide was used for diffraction experiments for the first time."],["dc.identifier.doi","10.1107/S0021889811049132"],["dc.identifier.gro","3142584"],["dc.identifier.isi","000299206400010"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/8951"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0021-8898"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray optics"],["dc.subject.gro","x-ray scattering"],["dc.title","A two-dimensional waveguide beam for X-ray nanodiffraction"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]Details DOI WOS2001Journal Article [["dc.bibliographiccitation.firstpage","139"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Psychiatrische Praxis"],["dc.bibliographiccitation.lastpage","143"],["dc.bibliographiccitation.volume","28"],["dc.contributor.author","Holzinger, A."],["dc.contributor.author","Mueller, P."],["dc.contributor.author","Priebe, S."],["dc.contributor.author","Angermeyer, M. C."],["dc.date.accessioned","2018-11-07T09:15:00Z"],["dc.date.available","2018-11-07T09:15:00Z"],["dc.date.issued","2001"],["dc.description.abstract","Objective: In a former quantitative study on relatives' beliefs about the causes of functional psychoses a preference for psychosocial explanations was found. There were also indications of an inclination towards a multicausal concept. The aim of this study is to try to replicate these findings using semi-qualitative methods. In addition, we are interested in knowing to what extent the causal beliefs of relatives are similar to those of the patients, Method: Problem-centered interviews were conducted with 31 relatives of schizophrenic patients. A computer-assisted qualitative content analysis was carried out with the transcripts of the interviews. Results: As in the previous study, relatives showed a strong propendency to endorse psychosocial explanations. However, among only one third a multicausal concept could be observed. The causal beliefs of relatives and patients were quite similar. Discussion: The findings are discussed with reference to the concept of subjective illness theory and the concepts social representations."],["dc.identifier.doi","10.1055/s-2001-12669"],["dc.identifier.isi","000168557000008"],["dc.identifier.pmid","11407258"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/27568"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Georg Thieme Verlag Kg"],["dc.relation.issn","0303-4259"],["dc.title","Causes of schizophrenia - Beliefs of patients' relatives"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]Details DOI PMID PMC WOS2001Journal Article [["dc.bibliographiccitation.firstpage","174"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Psychiatrische Praxis"],["dc.bibliographiccitation.lastpage","179"],["dc.bibliographiccitation.volume","28"],["dc.contributor.author","Holzinger, A."],["dc.contributor.author","Mueller, P."],["dc.contributor.author","Priebe, S."],["dc.contributor.author","Angermeyer, M. C."],["dc.date.accessioned","2018-11-07T09:08:46Z"],["dc.date.available","2018-11-07T09:08:46Z"],["dc.date.issued","2001"],["dc.description.abstract","Objective: According to a quantitative study on causal beliefs patients with functional psychoses favour psychosocial over biological explanations. They also tend to endorse a multicausal concept. The aim of this study is to try to replicate these findings using semi-qualitative methods. Methods: Problem-centered interviews were conducted with 78 schizophrenic patients consecutively discharged from inpatient or dayhospital treatment. Results: As in the previous study, patients provided more frequently psychosocial explanations. Biological causes were relatively rarely mentioned - mostly in response to suggestions of the interviewer. In addition, they were rather brief and not very elaborated. By contrast to the previous study, hardly any indications for a multicausal concept could be observed. Discussion: Patients' beliefs are contrasted with the results of psychiatric research on the causes of schizophrenia."],["dc.identifier.doi","10.1055/s-2001-13262"],["dc.identifier.isi","000168954400005"],["dc.identifier.pmid","11428303"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/26105"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Georg Thieme Verlag Kg"],["dc.relation.issn","0303-4259"],["dc.title","Causes of schizophrenia - Patients' viewpoint"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]Details DOI PMID PMC WOS2016Journal Article Research Paper [["dc.bibliographiccitation.firstpage","680"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","690"],["dc.bibliographiccitation.volume","110"],["dc.contributor.author","Bernhardt, Marten"],["dc.contributor.author","Priebe, Marius"],["dc.contributor.author","Osterhoff, Markus"],["dc.contributor.author","Wollnik, Bernd"],["dc.contributor.author","Diaz, Ana"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","Rehfeldt, Florian"],["dc.date.accessioned","2017-09-07T11:54:39Z"],["dc.date.available","2017-09-07T11:54:39Z"],["dc.date.issued","2016"],["dc.description.abstract","Adult human mesenchymal stem cells show structural rearrangements of their cytoskeletal network during mechanically induced differentiation toward various cell types. In particular, the alignment of acto-myosin fibers is cell fate-dependent and can serve as an early morphological marker of differentiation. Quantification of such nanostructures on a mesoscopic scale requires high-resolution imaging techniques. Here, we use small-angle x-ray scattering with a spot size in the micro- and submicrometer range as a high-resolution and label-free imaging technique to reveal structural details of stem cells and differentiated cell types. We include principal component analysis into an automated empirical analysis scheme that allows the local characterization of oriented structures. Results on freeze-dried samples lead to quantitative structural information for all cell lines tested: differentiated cells reveal pronounced structural orientation and a relatively intense overall diffraction signal, whereas naive human mesenchymal stem cells lack these features. Our data support the hypothesis of stem cells establishing ordered structures along their differentiation process."],["dc.identifier.doi","10.1016/j.bpj.2015.12.017"],["dc.identifier.gro","3141731"],["dc.identifier.isi","000369467800017"],["dc.identifier.pmid","26840732"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14077"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/446"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1542-0086"],["dc.relation.issn","0006-3495"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.orgunit","Fakultät für Physik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights","CC BY 4.0"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","x-ray scattering"],["dc.title","X-Ray Micro- and Nanodiffraction Imaging on Human Mesenchymal Stem Cells and Differentiated Cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]Details DOI PMID PMC WOS2014Journal Article Research Paper [["dc.bibliographiccitation.artnumber","e90884"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Gorniak, Thomas"],["dc.contributor.author","Haraszti, Tamas"],["dc.contributor.author","Garamus, Vasyl M."],["dc.contributor.author","Buck, Andreas R."],["dc.contributor.author","Senkbeil, Tobias"],["dc.contributor.author","Priebe, Marius"],["dc.contributor.author","Hedberg-Buenz, Adam"],["dc.contributor.author","Koehn, Demelza"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","Grunze, Michael"],["dc.contributor.author","Anderson, Michael G."],["dc.contributor.author","Rosenhahn, Axel"],["dc.date.accessioned","2017-09-07T11:46:25Z"],["dc.date.available","2017-09-07T11:46:25Z"],["dc.date.issued","2014"],["dc.description.abstract","Melanosomes are highly specialized organelles that produce and store the pigment melanin, thereby fulfilling essential functions within their host organism. Besides having obvious cosmetic consequences-determining the color of skin, hair and the iris-they contribute to photochemical protection from ultraviolet radiation, as well as to vision (by defining how much light enters the eye). Though melanosomes can be beneficial for health, abnormalities in their structure can lead to adverse effects. Knowledge of their ultrastructure will be crucial to gaining insight into the mechanisms that ultimately lead to melanosome-related diseases. However, due to their small size and electron-dense content, physiologically intact melanosomes are recalcitrant to study by common imaging techniques such as light and transmission electron microscopy. In contrast, X-ray-based methodologies offer both high spatial resolution and powerful penetrating capabilities, and thus are well suited to study the ultrastructure of electron-dense organelles in their natural, hydrated form. Here, we report on the application of small-angle X-ray scattering-a method effective in determining the three-dimensional structures of biomolecules - to whole, hydrated murine melanosomes. The use of complementary information from the scattering signal of a large ensemble of suspended organelles and from single, vitrified specimens revealed a melanosomal sub-structure whose surface and bulk properties differ in two commonly used inbred strains of laboratory mice. Whereas melanosomes in C57BL/6J mice have a well-defined surface and are densely packed with 40-nm units, their counterparts in DBA/2J mice feature a rough surface, are more granular and consist of 60-nm building blocks. The fact that these strains have different coat colors and distinct susceptibilities to pigment-related eye disease suggest that these differences in size and packing are of biological significance."],["dc.identifier.doi","10.1371/journal.pone.0090884"],["dc.identifier.fs","606505"],["dc.identifier.gro","3142172"],["dc.identifier.isi","000332845300034"],["dc.identifier.pmid","24621581"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/10014"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/5332"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1932-6203"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.orgunit","Fakultät für Physik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights","CC BY 4.0"],["dc.subject.gro","x-ray scattering"],["dc.title","Nano-Scale Morphology of Melanosomes Revealed by Small-Angle X-Ray Scattering"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]Details DOI PMID PMC WOS2002Journal Article [["dc.bibliographiccitation.firstpage","154"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Psychiatrische Praxis"],["dc.bibliographiccitation.lastpage","159"],["dc.bibliographiccitation.volume","29"],["dc.contributor.author","Holzinger, A."],["dc.contributor.author","Mueller, P."],["dc.contributor.author","Priebe, S."],["dc.contributor.author","Angermeyer, M. C."],["dc.date.accessioned","2018-11-07T10:31:03Z"],["dc.date.available","2018-11-07T10:31:03Z"],["dc.date.issued","2002"],["dc.description.abstract","Objective: This study is aimed at exploring what schizophrenic patients and their closest relatives expect the further course of the illness will be like and how it can be influenced. Method: Problem-centered interviews were conducted with 31 schizophrenic patients and their closest relatives at discharge from hospital treatment. The verbatim transcripts of the interviews were analyzed by means of structuring qualitative content analysis. Results: Most frequently, the respondents expected an improvement of the illness or they were unsure whether the actual recovery from psychosis will persist or whether a further relapse will occur. Less frequently, the respondents were sure that this will be the case or that the illness will take a chronic, eventually deteriorating course. The majority was convinced that the course of the illness can be influenced, by psychiatric treatment as well as by the patients themselves. As concerns the social situation in the near future, particularly changes of the occupational status were hoped for. Conclusion: The assessment of the prognosis of the schizophrenic illness by most patients and relatives appears quite realistic. Rather than considering themselves as helpless victims they see opportunities for influencing the further course of the illness. This should have a positive influence on the readiness to use mental health service and to Comply With treatment recommendations."],["dc.identifier.doi","10.1055/s-2002-25104"],["dc.identifier.isi","000175247300009"],["dc.identifier.pmid","11961717"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/44007"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Georg Thieme Verlag Kg"],["dc.relation.issn","0303-4259"],["dc.title","Prognosis of schizophrenia from the viewpoint of patients and relatives An explorative study"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]Details DOI PMID PMC WOS2014Journal Article Research Paper [["dc.bibliographiccitation.firstpage","2662"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","2673"],["dc.bibliographiccitation.volume","107"],["dc.contributor.author","Priebe, Marius"],["dc.contributor.author","Bernhardt, Marten"],["dc.contributor.author","Blum, Christoph"],["dc.contributor.author","Tarantola, Marco"],["dc.contributor.author","Bodenschatz, Eberhard"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2017-09-07T11:45:22Z"],["dc.date.available","2017-09-07T11:45:22Z"],["dc.date.issued","2014"],["dc.description.abstract","We have performed scanning x-ray nanobeam diffraction experiments on single cells of the amoeba Dictyostelium discoideum. Cells have been investigated in 1), freeze-dried, 2), frozen-hydrated (vitrified), and 3), initially alive states. The spatially resolved small-angle x-ray scattering signal shows characteristic streaklike patterns in reciprocal space, which we attribute to fiber bundles of the actomyosin network. From the intensity distributions, an anisotropy parameter can be derived that indicates pronounced local variations within the cell. In addition to nanobeam small-angle x-ray scattering, we have evaluated the x-ray differential phase contrast in view of the projected electron density. Different experimental aspects of the x-ray experiment, sample preparation, and data analysis are discussed. Finally, the x-ray results are correlated with optical microscopy (differential phase contrast and confocal microscopy of mutant strains with fluorescently labeled actin and myosin II), which have been carried out in live and fixed states, including optical microscopy under cryogenic conditions."],["dc.identifier.doi","10.1016/j.bpj.2014.10.027"],["dc.identifier.fs","606778"],["dc.identifier.gro","3142000"],["dc.identifier.isi","000345859500026"],["dc.identifier.pmid","25468345"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12127"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/3434"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: Deutsche Forschungsgemeinschaft [A11]; Sonderforschungsbereich [937]"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1542-0086"],["dc.relation.issn","0006-3495"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.orgunit","Fakultät für Physik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights","CC BY-NC-ND 3.0"],["dc.rights.uri","http://creativecommons.org/licenses/by-nc-nd/3.0/"],["dc.subject.gro","x-ray scattering"],["dc.subject.mesh","Anisotropy"],["dc.subject.mesh","Cell Survival"],["dc.subject.mesh","Dictyostelium"],["dc.subject.mesh","Nanoparticles"],["dc.subject.mesh","Scattering, Small Angle"],["dc.subject.mesh","Software"],["dc.subject.mesh","X-Ray Diffraction"],["dc.title","Scanning X-Ray Nanodiffraction on Dictyostelium discoideum"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]Details DOI PMID PMC WOS